Optimization of Ultrasonic Extraction of Polysaccharides in Lingyun<italic/> Pekoe and Its Antioxidant Activity

XIAN Liqing; LI Shan; FENG Bin; LIANG Jian; LIU Xiaofeng

doi:10.13386/j.issn1002-0306.2021080117

Volume 43 Issue 9

Apr. 2022

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Science and Technology of Food Industry > 2022 > 43(9): 178-184. > DOI: 10.13386/j.issn1002-0306.2021080117

XIAN Liqing, LI Shan, FENG Bin, et al. Optimization of Ultrasonic Extraction of Polysaccharides in Lingyun Pekoe and Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2022, 43(9): 178−184. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021080117.

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Optimization of Ultrasonic Extraction of Polysaccharides in Lingyun Pekoe and Its Antioxidant Activity

XIAN Liqing^{1, 2,},
LI Shan^{1, 3, ,},
FENG Bin^{1, 3},
LIANG Jian^{1, 3},
LIU Xiaofeng^{1, 3}

1.
Key Laboratory of Regional Ecological Environment Analysis and Pollution Control of West Guangxi, Baise 533000, China
2.
School of Materials Science and Engineering, Baise University, Baise 533000, China
3.
School of Chemistry and Environment Engineering, Baise University, Baise 533000, China

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Received Date: August 10, 2021
Available Online: March 07, 2022

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Abstract

Abstract

Lingyun pekoe as starting material, studies were carried out to optimize the extracting technology of polysaccharides, and investigate its antioxidant activity. Extracting yield of polysaccharides as the index, the extraction conditions were optimized by Box-Bohnken method based on the results of single-factor experiments. The antioxidant activities were evaluated by the determination of hydroxyl radical (·OH), 1,1-Diphenyl-2-picrylhydrazyl radical (DPPH·) , and inhibition effect on auto-oxidation of grease. Experimental results showed that, the optimal extraction conditions were obtained as follows: liquid-material ratio 69:1 mL/g, temperature 52 ℃, ultrasonic time 21 min, ultrasonic power 180 W. Under the optimal conditions, an average extraction yield (4.84%±0.04%) was obtained, which was similar with the expected yield 5.06% (<5%) of regressive equation. The IC₅₀ of polysaccharides for eliminating ·OH, DPPH· were 0.262, 0.438 mg/mL, and a better clearance result would be obtained with higher concentration. Moreover, polysaccharides could prolong the storage time of grease in normal circumstance for inhibiting the auto-oxidation. The studies mentioned above indicated that polysaccharides were plentiful in Lingyun poeke, and had a good antioxidant activity, providing data support for promoting in home and abroad.
- Lingyun pekoe,
- polysaccharides,
- ultrasonic-assisted extraction,
- Box-Bohnken method,
- antioxidant activity

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