Optimization of Ultrafiltration Process of Yellow Serofluid by Response Surface Methodology and Its Metabolomics Analysis

WANG Minshen; LI Yong; FENG Jin; LI Chunyang; HOU Wanwei; OLUWOLE Oluwatoyin Bolanle; CHUON Mony Roth

doi:10.13386/j.issn1002-0306.2021080098

Volume 43 Issue 7

Mar. 2022

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Science and Technology of Food Industry > 2022 > 43(7): 224-232. > DOI: 10.13386/j.issn1002-0306.2021080098

WANG Minshen, LI Yong, FENG Jin, et al. Optimization of Ultrafiltration Process of Yellow Serofluid by Response Surface Methodology and Its Metabolomics Analysis[J]. Science and Technology of Food Industry, 2022, 43(7): 224−232. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021080098.

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Optimization of Ultrafiltration Process of Yellow Serofluid by Response Surface Methodology and Its Metabolomics Analysis

1.
College of Food Engineering, Harbin University of Commerce, Harbin 150076, China
2.
Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
3.
Institute of Agricultural Re-sources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
4.
Qinghai Academy of Agricultural and Forestry Sciences, Xining 810000, China
5.
Food Technology Department, Oshodi Federal Institute Industrial Research , Lagos 999062, Nigeria
6.
Cambodia Laboratory of Agricultural Products and Foods, Ministry of Agriculture Forestry and Fisheries of Cambodia, Phnom Penh 10103, Kingdom of Cambodia

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Received Date: August 10, 2021
Available Online: February 09, 2022

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Abstract

Abstract

Ultrafiltration separation technology was used to concentrate yellow serofluid in order to obtain protein efficiently and reduce the pollution of yellow serofluid and improve its comprehensive utilization. On the basis of single factor experiment, the ultrafiltration process of yellow serofluid was optimized by response surface. The contents of protein and total sugar, biochemical oxygen demand(BOD), chemical oxygen demand(COD) and metabolomics of yellow serofluid before and after ultrafiltration were determined. The results showed that the optimal parameters of ultrafiltration concentration of yellow serofluid were as follows: pH6.2, operating pressure 0.22 MPa, temperature 31 ℃. The membrane flux was (51.8±1.3) L/m²·h, the protein interception rate was 76.84%, the total sugar transmittance rate was 79.10%, and the removal rates of BOD and COD were 69.27% and 61.03% respectively. Metabolomics analysis of the concentrated and translucent solutions showed that there were significant differences in the contents of 114 compounds in the concentrated and translucent solutions. The results show that ultrafiltration can effectively separate proteins and sugars from yellow serofluid, and has a certain enrichment effect on small molecules, which provides a certain reference for the further development and utilization of yellow serofluid.
- yellow serofluid,
- ultrafiltration,
- process optimization,
- metabolomics analysis,
- proteins,
- carbohydrates

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