Protective Effect of <i>Polygonatum odoratum</i> Polysaccharides on A7r5 Cell Senescence Induced by D-Galactose

LI Minghui; HOU Junyu; HU Di; LIU Jing; ZHAO Mengdan; GE Junhong; SHEN Ye; LI Tan; PENG Yubo; LIU Peng; SUN Xin

doi:10.13386/j.issn1002-0306.2021070322

Volume 43 Issue 6

Mar. 2022

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Science and Technology of Food Industry > 2022 > 43(6): 380-388. > DOI: 10.13386/j.issn1002-0306.2021070322

LI Minghui, HOU Junyu, HU Di, et al. Protective Effect of Polygonatum odoratum Polysaccharides on A7r5 Cell Senescence Induced by D-Galactose[J]. Science and Technology of Food Industry, 2022, 43(6): 380−388. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021070322.

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Protective Effect of Polygonatum odoratum Polysaccharides on A7r5 Cell Senescence Induced by D-Galactose

LI Minghui^{1, 2,},
HOU Junyu^{1, 2},
HU Di^{1, 2},
LIU Jing^{1, 2},
ZHAO Mengdan^{1, 2},
GE Junhong³,
SHEN Ye⁴,
LI Tan⁴,
PENG Yubo²,
LIU Peng^5, ,,
SUN Xin^2, ,

1.
College of Basic Medicine, Beihua University, Jilin 132013, China
2.
College of Pharmacy, Jilin Medical College, Jilin 132013, China
3.
College of Pharmacy, Beihua University, Jilin 132013, China
4.
College of Public Health, Beihua University, Jilin 132013, China
5.
College of Stomatology, Beihua University, Jilin 132013, China

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Received Date: July 26, 2021
Available Online: January 11, 2022

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Abstract

Abstract

Objective: The protective effect of Polygonatum odoratum polysaccharides (POP) on D-galactose (D-gal) induced senescence in rat aortic smooth muscle cells (A7r5) was investigated. Methods: Firstly, the POP was extracted by the water extraction and alcohol precipitation methods. Next, the phenol concentrated sulfuric acid method was applied to further measure the total sugar content of POP. In addition, the A7r5-senescent cell model was established by the D-galactose treatment. Finally, the MTT assay, β-galactosidase staining, reactive oxygen species (ROS) staining as well as JC-1 staining were applied to further detect the protective effect of POP in A7r5-senescent cells induced by D-galactose. Results: This study found an yield of POP was 8.23% and its total sugar content was 76.48%±0.036%. As a result, the MTT assay showed the viability of the A7r5 cells was increased to 174.89%±3.30% after 24 h treatment by 400 μg/mL of POP, while it was decreased to 65.93%±1.63% after 48 h treatment by 40 mg/mL of D-galactose. Interestingly, when a concentration of POP was 100 μg/mL, this study had found the most significant protective effect in A7r5-senescent cells induced by D-galactose, which increased its cell viability to 226.87%±12.58% as compared with the control group (P<0.001). Additionally, POP decreased the total β-galactosidase, inhibited its ROS levels, increased the number of positive cells after JC-1 staining, as well as protected the stability of mitochondrial membrane potential in A7r5 cells induced by D-galactose. Conclusion: POP had a potential role in the suppression of oxidative stress and cellular senescence in A7r5 cells induced by D-galactose, which could further support its anti-aging effects in vitro.
- Polygonatum odoratum polysaccharide,
- cell senescence,
- β-galactosidase,
- reactive oxygen species,
- mitochondrial membrane potential

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References

[1]	KUČEROVÁ Z, RÁC M, MIKULÍK J, et al. The anti-senescence activity of cytokinin arabinosides in wheat and arabidopsis is negatively correlated with ethylene production[J]. Int J Mol Sci,2020,21(21):8109. doi: 10.3390/ijms21218109
[2]	CHO E G, CHOI S Y, KIM H, et al. Panax ginseng-derived extracellular vesicles facilitate anti-senescence effects in human skin cells: An eco-friendly and sustainable way to use ginseng substances[J]. Cells,2021,10(3):486. doi: 10.3390/cells10030486
[3]	GAMBINO V, DE M G, VENEZIA O, et al. Oxidative stress activates a specific p53 transcriptional response that regulates cellular senescence and aging[J]. Aging Cell,2013,12:435−445. doi: 10.1111/acel.12060
[4]	POROPATICH K, FONTANAROSA J, SAMANT S, et al. Cancer immunotherapies: Are they as effective in the elderly?[J]. Drugs Aging,2017,34(8):567−581. doi: 10.1007/s40266-017-0479-1
[5]	LARA J, SHERRATT M J, REES M. Aging and anti-aging[J]. Maturitas,2016,93:1−3. doi: 10.1016/j.maturitas.2016.08.020
[6]	HERNANDEZ S A, NEHME J, DEMNRIA M. Hallmarks of cellular senescence[J]. Trends Cell Biol,2018,28:436−453. doi: 10.1016/j.tcb.2018.02.001
[7]	GURAU F, BALDNOI S, PRATTICHIZZO F, et al. Anti-senescence compounds: A potential nutraceutical approach to healthy aging[J]. Ageing Res Rev,2018,46:14−31. doi: 10.1016/j.arr.2018.05.001
[8]	ZHAO P, ZHAO C C, LI X, et al. The genus Polygonatum: A review of ethnopharmacology, phytochemistry and pharmacology[J]. J Ethnopharmacol,2018,214:274−291. doi: 10.1016/j.jep.2017.12.006
[9]	赵容, 许亮, 谢明, 等. 中药玉竹的本草考证[J]. 中国实验方剂学杂志,2017,15:227−234. [ZHAO R, XU L, XIE M, et al. Textual research of Polygonati odorati rhizoma[J]. Chinese Journal of Experimental Traditional Medical Formulae,2017,15:227−234.
[10]	CUI X W, WANG S Y, CAO H, et al. A review: The bioactivities and pharmacological applications of Polygonatum sibiricum polysaccharides[J]. Molecules,2018,23(5):1170. doi: 10.3390/molecules23051170
[11]	桑野, 李晓光, 张昌浩, 等. 国内植物多糖的研究及发展趋势[J]. 吉林医药学院学报,2017,38(6):455−457. [SANG Y, LI X G, ZHANG C H, et al. Research and development trend of domestic plant polysaccharides[J]. Journal of Jilin Medical College,2017,38(6):455−457.
[12]	余江南, 姜慧妍, 徐遥, 等. 玉竹多糖的结构及其生物活性研究进展[J]. 现代食品科技,2018,34(8):273−282,144. [YU J N, JIANG H Y, XU Y, et al. Research progress on the structure and biological activity of Polygonatum odoratum polysaccharides[J]. Modern Food Science and Technology,2018,34(8):273−282,144.
[13]	李耀光, 曹珂, 罗灿选. 玉竹多糖的组成及其体外抗氧化活性研究[J]. 食品工业,2018,39(5):184−186. [LI Y G, CAO K, LUO C X. Study on the composition of Polygonatum odoratum polysaccharide and its in vitro antioxidant activity[J]. Food Industry,2018,39(5):184−186.
[14]	宁慧, 李会宁, 杨培君. 玉竹多糖的抗氧化作用研究[J]. 陕西理工学院学报(自然科学版),2013,29(6):59−65. [NING H, LI H N, YANG P J. Study on the antioxidant effect of Polygonatum odoratum polysaccharides[J]. Journal of Shaanxi Institute of Technology (Natural Science Edition),2013,29(6):59−65.
[15]	李化强, 吴菲菲, 龙艳珍, 等. 湘玉竹多糖的体外抗氧化活性研究[J]. 食品安全质量检测学报,2017,8(8):3062−3067. [LI H Q, WU F F, LONG Y Z, et al. Study on the in vitro antioxidant activity of the polysaccharides from Xiangyu bamboo[J]. Journal of Food Safety and Quality Inspection,2017,8(8):3062−3067. doi: 10.3969/j.issn.2095-0381.2017.08.037
[16]	彭壮, 李弘, 彭莎, 等. 玉竹多糖对D-半乳糖诱导的衰老小鼠认知障碍的改善[J]. 中华中医药学刊,2020,38(11):8. [PENG Z, LI H, PENG S, et al. Polygonatum odoratum polysaccharides improve cognitive impairment in D-galactose-induced aging mice[J]. Chinese Journal of Traditional Chinese Medicine,2020,38(11):8.
[17]	LI B, WU P P, FU W W, et al. Polygonatum sibiricum the role and mechanism of miRNA-1224 in the polysaccharide regulation of bone marrow-derived macrophages to osteoclast differentiation[J]. Rejuvenation Res,2019,22:420−430. doi: 10.1089/rej.2018.2126
[18]	仇宏伟. 超声波辅助酶法提取玉竹多糖工艺研究[J]. 青岛农业大学学报(自然科学版),2020,37(1):38−42,52. [CHOU H W. Study on the technology of ultrasonic-assisted enzymatic extraction of polysaccharides from Polygonatum odoratum[J]. Journal of Qingdao Agricultural University (Natural Science Edition),2020,37(1):38−42,52.
[19]	李素红, 王昭曦, 姜晓坤, 等. 超声波辅助法提取玉竹多糖工艺优化研究[J]. 东北农业科学,2018,43(3):61−64. [LI S H, WANG Z X, JIANG X K, et al. Study on the optimization of extraction technology of Polygonatum odoratum polysaccharides assisted by ultrasonic[J]. Northeast Agricultural Sciences,2018,43(3):61−64.
[20]	陈银霞. 玉竹粗多糖水提醇沉法提取工艺的优化[J]. 黑龙江畜牧兽医,2013(17):138−139. [CHEN Y X. Optimization of the extraction process of Polygonatum odoratum crude polysaccharide by water extraction and alcohol precipitation[J]. Heilongjiang Animal Science and Veterinary Medicine,2013(17):138−139.
[21]	刘玉凤, 李霞, 许丽丽, 等. 玉竹多糖含量测定及其生物活性研究[J]. 时珍国医国药,2015,26(11):2589−2591. [LIU Y F, LI X, XU L L, et al. Determination of polysaccharides of Polygonatum odoratum and its biological activity[J]. Lishizhen Medicine and Materia Medica,2015,26(11):2589−2591.
[22]	张倩倩, 刘丽骏, 徐盼, 等. D-半乳糖诱导正常人口腔黏膜角质形成细胞衰老的研究[J]. 中华老年口腔医学杂志,2020,18(6):337−341. [ZHANG Q Q, LIU L J, XU P, et al. D-galactose induces senescence of normal human oral mucosal keratinocytes[J]. Chinese Journal of Geriatric Stomatology,2020,18(6):337−341.
[23]	乔巨慧, 刘颖, 邢欣, 等. 人参多糖对D-半乳糖诱导的PC12细胞拟老化模型自噬和相关信号通路的影响[J]. 中药材,2020(10):2519−2525. [QIAO J H, LIU Y, XING X, et al. The effect of ginseng polysaccharides on autophagy and related signal pathways in PC12 cell aging model induced by D-galactose[J]. Chinese Medicinal Materials,2020(10):2519−2525.
[24]	曾立, 向荣. 基于D-半乳糖致衰老鼠模型的抗衰老药物研究进展[J]. 邵阳学院学报(自然科学版),2018,15(6):108−116. [ZENG L, XIANG R. Research progress of anti-aging drugs based on D-galactose-induced aging mouse model[J]. Journal of Shaoyang University (Natural Science Edition),2018,15(6):108−116.
[25]	WANG J, LIU L, DING Z J, et al. Exogenous NAD postpones the D-gal-induced senescence of bone marrow-derived mesenchymal stem cells via Sirt1 signaling[J]. Antioxidants (Basel),2021,10(2):254. doi: 10.3390/antiox10020254
[26]	AHMAD S, KHAN A, ALI W, et al. Fisetin rescues the mice brains against D-galactose-iduced oxidative stress, neuroinflammation and memory impairment[J]. Front Pharmacol,2021,12:612078. doi: 10.3389/fphar.2021.612078
[27]	LIU R Y, MENG J, LOU D F. Adiponectin inhibits D-gal-induced cardiomyocyte senescence via AdipoR1/APPL1[J]. Mol Med Rep,2021,24(4):719. doi: 10.3892/mmr.2021.12358
[28]	蒋春茂, 陈晓兰, 陆广富, 等. 不同中药多糖体外对鸡外周血和脾脏淋巴细胞增殖能力的比较[J]. 江苏农业学报,2015,31(1):106−111. [JIANG C M, CHEN X L, LU G F, et al. Comparison of the proliferation of chicken peripheral blood and spleen lymphocytes in vitro by polysaccharides from different Chinese medicines[J]. Jiangsu Journal of Agriculture,2015,31(1):106−111. doi: 10.3969/j.issn.1000-4440.2015.01.016
[29]	王迦琦, 许梦然, 高婧文, 等. 北虫草多糖提取工艺优化及其细胞氧化损伤保护作用[J]. 食品工业科技,2020,41(13):141−147. [WANG J Q, XU M R, GAO J W, et al. Optimization of the extraction process of Cordyceps militaris polysaccharides and its protective effect on cell oxidative damage[J]. Science and Technology of Food Industry,2020,41(13):141−147.
[30]	DONG R C, WANG X H, WANG L, et al. Yangonin inhibits ethanol-induced hepatocyte senescence via miR-194/FXR axis[J]. Eur J Pharmacol,2020,890:173653.
[31]	刘琪梦. 线粒体靶向的苯硫酚荧光探针、衰老相关β—半乳糖苷酶荧光探针的构建以及生物成像研究[D]. 西安: 西北大学, 2019. LIU Q M. The construction of thiophenol fluorescent probes targeting mitochondria and the construction of senescence-related β-galactosidase fluorescent probes and bioimaging research[D]. Xi’an: Northwest University, 2019.
[32]	吴爽, 李学锋, 王秋彤, 等. 发酵黄芪对人胚肺二倍体成纤维细胞增殖及衰老相关蛋白表达的影响[J]. 长春中医药大学学报,2020,36(4):676−679. [WU S, LI X F, WANG Q T, et al. Effects of fermented Astragalus on the proliferation and senescence-related protein expression of human embryonic lung diploid fibroblasts[J]. Journal of Changchun University of Traditional Chinese Medicine,2020,36(4):676−679.
[33]	邢玉芝. D-半乳糖诱导生物体衰老与细胞衰老模型的实验研究[D]. 成都: 四川大学, 2006. XING Y Z. Experimental study of D-galactose-induced biological senescence and cellular senescence model[D]. Chengdu: Sichuan University, 2006.
[34]	SUN K, YANG P, ZHAO R, et al. Matrine attenuates D-galactose-induced aging-related behavior in mice via inhibition of cellular senescence and oxidative stress[J]. Oxid Med Cell Longev,2018:7108604.
[35]	SCHERLINGER M, TSOKOS G. Reactive oxygen species: The Yin and Yang in (auto-) immunity[J]. Autoimmun Rev,2021:102869.
[36]	GREFTE S, KOOPMAN W J H. Live-cell assessment of reactive oxygen species levels using dihydroethidine[J]. Methods Mol Biol,2021,2275:291−299.
[37]	HE F L, LIU Q, JING M Y, et al. Toxic mechanism on phenanthrene-induced cytotoxicity, oxidative stress and activity changes of superoxide dismutase and catalase in earthworm (Eisenia foetida): A combined molecular and cellular study[J]. J Hazard Mater,2021,418:126302. doi: 10.1016/j.jhazmat.2021.126302
[38]	许梦然, 王迦琦, 高婧雯, 等. 北五味子多糖提取工艺优化及其对LPS刺激巨噬细胞线粒体膜电位的保护作用[J]. 食品工业科技,2020,41(20):33−40. [XU M R, WANG J A, GAO J W, et al. Optimization of the extraction process of Schisandra chinensis polysaccharide and its protective effect on LPS stimulated macrophage mitochondrial membrane potential[J]. Science and Technology of Food Industrial,2020,41(20):33−40.
[39]	官福新, 周露露, 张宸豪, 等. JC-1单染法检测CCCP对内皮细胞线粒体膜电位的影响[J]. 吉林医药学院学报,2014,35(5):324−326. [GUAN F X, ZHOU L L, ZHANG C H, et al. JC-1 single staining method to detect the effect of CCCP on endothelial cell mitochondrial membrane potential[J]. Journal of Jilin Medical College,2014,35(5):324−326.
[40]	宋兴瑰. 促排卵周期未成熟卵母细胞体外成熟的发育潜能研究及其线粒体膜电位检测[D]. 济南: 山东大学, 2015. SONG X G. Study on the developmental potential of immature oocytes in ovulation induction cycle and detection of mitochondrial membrane potential[D]. Jinan: Shandong University, 2015.