Antioxidant, Hypoglycemic Activities and Inhibitory Effect on Tyrosinase of Ethanol Extracts of Different Parts from <i>Psidium guajava</i> L.

ZHUANG Yuanbei; WEI Aihong; CHENG Kanbin; ZHANG Chao; LIU Fuwei; LIANG Shuhe; ZHANG Shengyuan

doi:10.13386/j.issn1002-0306.2021070263

Volume 43 Issue 8

Apr. 2022

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Science and Technology of Food Industry > 2022 > 43(8): 365-371. > DOI: 10.13386/j.issn1002-0306.2021070263

ZHUANG Yuanbei, WEI Aihong, CHENG Kanbin, et al. Antioxidant, Hypoglycemic Activities and Inhibitory Effect on Tyrosinase of Ethanol Extracts of Different Parts from Psidium guajava L. DOI: 10.13386/j.issn1002-0306.2021070263

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Antioxidant, Hypoglycemic Activities and Inhibitory Effect on Tyrosinase of Ethanol Extracts of Different Parts from Psidium guajava L.

1.
Guangdong Provincial Key Laboratory of Conservation and Precision Utilization of Characteristic Agricultural Resources in Mountainous Areas, Jiaying University, Meizhou 514015, China
2.
Institute of Hakka Medicinal Bio-resources, Medical College, Jiaying University, Meizhou 514031, China

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Received Date: July 22, 2021
Available Online: February 20, 2022

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Abstract

Abstract

Objective: The antioxidant, hypoglycemic and tyrosinase inhibitory activities of ethanol extracts of different parts (root, stem, leave and fruit) from Psidium guajava L. were compared. Methods: The free radical scavenging were determined by DPPH and ABTS method. The hypoglycemic activities were determined by pNPG and DNS method. The inhibition of tyrosinase activity was determined by L-DOPA method. Results: The ethanol extracts of different parts from Psidium guajava L. had certain antioxidant, hypoglycemic activities and inhibitory effect on tyrosinase, and there were dose effect relationship. The ethanol extracts of the root from Psidium guajava L. had the strongest activity, and its free radical scavenging of ABTS⁺ and DPPH was close to V_C, its IC₅₀ values of the scavenging ability of ABTS⁺ and DPPH free radicals were (8.45±0.12), (0.09±0.002) mg/mL, and its inhibitory strength on yeast α-glucosidase and α-amylase was higher than that of positive control acarbose. And its IC₅₀ values of the inhibitory activity on α-amylase and yeast α-glucosidase were: (0.10±0.02), (8.74±0.25) μg/mL, and the inhibitory activity of tyrosinase was weaker than V_C. Conclusion: The ethanol extract of the root from Psidium guajava L. had the highest antioxidant, hypoglycemic activities and inhibitory effect on tyrosinase, which suggested that the root from Psidium guajava L. is a potential inhibitors resource of antioxidant, α-glucosidase, α-amylase and tyrosinase.
- Psidium guajava L.,
- antioxidant,
- hypoglycemic activities,
- tyrosinase

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References

[1]	李坤, 张伊晗, 郭宇萱, 等. 番石榴叶的抗二型糖尿病活性成分研究进展[J]. 当代化工研究,2018(5):163−164. [LI K, ZHANG Y H, GUO Y X, et al. Research progress in the active components of Guava leaf against type diabetes mellitus[J]. Chenmical Intermediate,2018(5):163−164. doi: 10.3969/j.issn.1672-8114.2018.05.101
[2]	南京中医药大学. 中药大辞典[M]. 上海: 上海科学技术出版社, 2006: 3371-3373. Nanjing University of Chinese Medicine. Dictionary of traditional Chinese medicine [M]. Shanghai: Shanghai Science and Technology Press, 2006.
[3]	彭财英, 黄应正, 刘建群, 等. 番石榴根中一个新的三萜类成分[J]. 药学学报,2017,52(11):1731−1736. [PENG C Y, HUANG Y Z, LIU J Q, et al. A new triterpenoid from roots of Psidium guajava[J]. Acta Pharmaceutica Sinica,2017,52(11):1731−1736.
[4]	方皓, 熊素琴, 燕娜娜, 等. 一测多评法测定番石榴叶中6种黄酮类成分的含量[J]. 天然产物研究与开发,2019,31(9):1559−1566. [FANG H, XIONG S Q, YAN N N, et al. Simultaneous determination of six flavonoids in Psidium guajava Linn. by QAMS[J]. Natural Product Research and Development,2019,31(9):1559−1566.
[5]	汪梅花, 魏文浩, 吴振强. 番石榴叶的生物活性研究进展[J]. 中药材,2015,38(10):2215−2219. [WANG H H, WEI W H, WU Z Q. Research progress on bioactivity of Guava leaves[J]. Journal of Chinese Medicinal Materials,2015,38(10):2215−2219.
[6]	郭莹, 熊阳, 宋忠诚, 等. 番石榴叶挥发油的提取、成分分析及抑菌活性研究[J]. 中华中医药杂志,2015,30(10):3754−3757. [GUO Y, XIONG Y, SONG Z C, et al. Study on extraction technology, components analysis and antibacterial activity of Psidium guajava L[J]. China Journal of Traditional Chinese Medicine and Pharmacy,2015,30(10):3754−3757.
[7]	符春丽, 彭燕, 黎诗敏, 等. 番石榴叶乙酸乙酯萃取物的体外抗氧化活性及化学成分的分离鉴定[J]. 现代食品科技,2017,33(10):52−57. [FU C L, PENG Y, LI S M, et al. Isolation and identification of chemical constituents and in vitro antioxidant activity of ethyl acetate extracts from Psidium guajava Linn leaves[J]. Modern Food Science and Technology,2017,33(10):52−57.
[8]	吕冰冰, 谢笔钧, 孙智达. 红肉番石榴果胶的理化特性及其体外降脂作用[J/OL]. 食品工业科技: 1−16 [2021-05-31]. https://doi.org/10.13386/j.issn1002-0306.2021010091. LV B B, XIE B J, SUN Z D. Physical and chemical properties of red-flesh guava pectin and its lipid-lowering effect in vitro[J/OL]. Science and Technology of Food Industry: 1−16 [2021-05-31].https://doi.org/10.13386/j.issn1002-0306.2021010091.
[9]	周浓, 莫日坚, 黄秋艳, 等. 番石榴多酚的提取纯化及其抑菌活性研究[J]. 食品与发酵工业,2020,46(14):182−188. [ZHOU N, MO R J, HUANGY Q, et al. Study on extraction and purification of guava polyphenols and its antibacterial activity[J]. Food and Fermentation Industries,2020,46(14):182−188.
[10]	刘晓聪, 林冬梅, 刘敏, 等. 番石榴的化学成分及其抗肿瘤与抗真菌活性[J/OL]. 中国中药杂志: 1−10 [2021-05-31]. https://doi.org/10.19540/j.cnki.cjcmm.20210517.202. LIU X C, LIN D M, LIU M, et al. Chemical constituents of Psidium guajava and their antitumor and antifungal activities[J/OL]. China Journal of Chinese Materia Medica: 1−10[2021-05-31]. https://doi.org/10.19540/j.cnki.cjcmm.20210517.202.
[11]	世界卫生组织国际癌症研究机构[EB/OL]. https: //www. iarc. fr/faq/latest-global-cancer-data-2020-qa/. -data-2020-qa/. International Agency for Research on Cancer[EB/OL]. https: //www. iarc. fr/faq/latest-global-cancer-data-2020-qa/. -data-2020-qa/.
[12]	International Diabetes Federation. IDF diabetes atlas, 9thed[EB/OL]. [2020-04-03]. http://www.diabetesatlas.org.
[13]	CDC. Centers for Disease Cotrol and Prevention, CancerPrevention and Control, State of the Science on MelanomaPrevention and Screening[EB/OL].https://www.cdc.gov/cancer/dcpc/re- search/articles/melanoma-risehtm.
[14]	刘冉, 程霜, 王雷, 等. 不同品种甘薯叶提取物抗氧化及对 α-葡萄糖苷酶抑制活性的研究[J]. 食品工业科技, 2019, 40(23): 283−289. LIU R, CHENG S, WANG L, et al. Antioxidant activity and α-glucosidase inhibitory activity of sweet potato (Ipomoea batatas L. ) leaf extracts from different varieties[J]. Science and Technology of Food Industry, 2019, 40(23): 283−289.
[15]	毕云枫, 宋凤瑞, 刘志强. 天然酪氨酸酶抑制剂的种类及其对酪氨酸酶抑制作用的研究进展[J]. 吉林大学学报(医学版),2014,40(2):454−459. [BI Y F, SONG F R, LIU Z Q. Research progress on types of natural tyrosinase inhibitors and their inhibitory effects on tyrosinase[J]. Journal of Jilin University (Medicine Edition),2014,40(2):454−459.
[16]	IGHODARO O M. Molecular pathways associated with oxidative stress in diabetes mellitus[J]. Biomedicine & Pharmacotherapy,2018,108:656−662.
[17]	姚志仁, 李豫, 朱开梅, 等. 黄花倒水莲不同极性部位抗氧化和降血糖活性研究[J]. 食品工业科技,2020,41(7):55−59. [YAO Z R, LI Y, ZHU K M, et al. Antioxidant and hypoglycemic activities of different parts partitioned from the ethanol extract of Polygala fallax Hemsl[J]. Science and Technology of Food Industry,2020,41(7):55−59.
[18]	成秋桂, 高丽群, 邓峰云, 等. 抗氧化在化妆品行业的应用进展[J]. 日用化学品科学,2019,42(2):32−38. [CHEN Q J, GAO L Q, DENG F Y, et al. Application of antioxidant in cosmetics industry[J]. Detergent & Cosmetics,2019,42(2):32−38. doi: 10.3969/j.issn.1006-7264.2019.02.010
[19]	李榕娣, 庄远杯, 魏爱红, 等. 不同蕨菜制品醇提取物体外抗氧化及降血糖活性研究[J]. 食品工业科技,2015,11(3):383−391. [LI R D, ZHUANG Y B, WEI A H, et al. Antioxidant and hypoglycemic activities of different processing products from ethanol extracts of Blechnum orientale L. in vitro[J]. Science and Technology of Food Industry,2015,11(3):383−391.
[20]	METROUH-AMIR H, DUARTE C M M, MAIZA F. Solvent effect on total phenolic contents, antioxidant, and antibacterial activities of Matricaria pubescens[J]. Industrial Crops and Products,2015,67:249−256. doi: 10.1016/j.indcrop.2015.01.049
[21]	LI J, ZHANG Q, CUI J, et al. Characterization and antioxidant activity of flash-assistedextracted dihydroquercetin from wood sawdust of Larix gmelinii using a response surface methodology[J] . International Journal of Food Engineering, 2016, 12(6): 587-597.
[22]	丘建媚, 李菲菲, 温正辉, 等. 琴叶榕根提取物对α-葡萄糖苷酶、α-淀粉酶的抑制作用[J]. 中国民族民间医药,2019,28(14):26−29. [QIU J M, LI F F, WEN Z H, et al. Study on the on α-glycosidase and α-amylase inhibitory activities of extract from the Ficus pandurata root[J]. Chinese Journal of Ethnomedicine and Ethnopharmacy,2019,28(14):26−29.
[23]	朱文佳, 寇自农, 张曦, 等. α-葡萄糖苷酶抑制剂体外筛选方法的研究[J]. 食品研究与开发,2012,33(8):171−175. [ZHU W J, KOU Z N, ZHANG X, et al. Study of screening method for α-glucosidase inhibitors in vitro[J]. Food Research and Development,2012,33(8):171−175. doi: 10.3969/j.issn.1005-6521.2012.08.052
[24]	张丙云, 苏丹, 郭涛, 等. 竹叶椒乙醇提取物对α-葡萄糖苷酶的抑制作用及其机理研究[J]. 食品工业科技,2015,36(21):345−348. [ZHANG B Y, SU D, GUO T, et al. Study on the inhibitory effect and inhibition mechanism on α-glycosidase of ethanol extract from Zanthoxylum armatum DC J]. Science and Technology of Food Industry,2015,36(21):345−348.
[25]	温正辉, 凌梅娣, 余思萍, 等. 蒲桃不同药用部位乙醇提取物对α-葡萄糖苷酶和α-淀粉酶活性的抑制作用研究[J]. 中国药房,2019,30(23):3246−3251. [WEN Z H, LIN M D, YU S P, et al. Study on inhibitory effects of ethanol extract of different medicinal parts from Syzygium jambos on the activities of α-glycosidase and α-amylase[J]. China Pharmacy,2019,30(23):3246−3251.
[26]	丁华杰, 叶云, 安欢, 等. 虎杖白藜芦醇的提取及抑制α-淀粉酶和α-葡萄糖苷酶活性的研究[J]. 中国调味品,2020,45(2):176−181. [DING H J, YE Y, AN H, et al. Extractio of resveratrol from Polygoum cuspidatum and its inhibition of α-amylase and α-glucosidase activity[J]. CHINA Condiment,2020,45(2):176−181. doi: 10.3969/j.issn.1000-9973.2020.02.040
[27]	MENG Y, SU A, YUAN S, et al. Evaluation of total flavonoids, myricetin, and quercetin from Hovenia dulcis Thunb. as inhibitors of α-amylase and α-glucosidase[J]. Plant Foods for Human Nutrition,2016,71(4):444−449. doi: 10.1007/s11130-016-0581-2
[28]	袁堂丰. 罗汉松化学成分分离及抑制酪氨酸酶活性研究[D]. 吉首: 吉首大学, 2019. YUAN T F. Isolation on chemical constituents from Podocarpus macrophllus and their tyrosinase inhibition activity[D]. Jishou: Jishou University, 2019.
[29]	孙熙浛, 齐欣, 崔承弼. 辐照红景天乙醇提取物的抗氧化作用及美白作用研究[J]. 食品工业科技,2020,41(23):325−331. [SUN X H, QI X, CUI C B. Antioxidant and whitening effects of ethanol extract from irradiated Rhodiola rosea[J]. Science and Technology of Food Industry,2020,41(23):325−331.
[30]	陈圣加, 黄应正, 卢健, 等. 番石榴根中酚酸类化学成分分离鉴定[J]. 中国实验方剂学杂志,2019,25(2):169−174. [CHEN S J, HUANG Y Z, LU J, et al. Phenolic components from roots of Psidium guajava[J]. Chinese Journal of Experimental Traditional Medical Formulae,2019,25(2):169−174.
[31]	SIRIVIBULKOVIT K, NOUANTHAVONG S, SAMEENOI Y. Paper-based DPPH assay for antioxidant activity analysis[J]. Analytical Sciences, 2018, 34(7): 795−800.
[32]	张婉君, 冯彬, 谢笔钧, 等. 白肉番石榴总黄酮提取工艺优化及体外抗氧化活性分析[J]. 食品工业科技,2019,40(8):196−201. [ZHANG W J, FENG B, XIE B J, et al. Optimization of extraction process and in vitro antioxidant activity analysis of total flavonoids from white-flesh Guava (Psidium guajava L. cv. Pearl)[J]. Science and Technology of Food Industry,2019,40(8):196−201.
[33]	戴卫波, 李乐愚, 梅全喜. 复方番石榴制剂对HepG2细胞胰岛素抵抗的改善及对α-淀粉酶和α-葡萄糖苷酶抑制作用[J]. 中药新药与临床药理,2016,27(5):655−660. [DAI W B, LI L Y, MEI Q X, et al. Improvement of insulin resistance in hepG2 cells and inhibition of α-amylase and α-glucosidase by compound Fructus psidii guajavae preparation[J]. Traditional Chinese Drug Research & Clinical Pharmacology,2016,27(5):655−660.
[34]	叶伟. 番石榴叶有机溶剂提取物清除过氧自由基能力及抑制酪氨酸酶活力研究[J]. 安徽农业科学,2020,48(2):186−188. [YE W. Study on scavenging peroxy radical activity and inhibitory effect on tyrosinase of Guava leaves extract[J]. Journal of Anhui Agricultural Sciences,2020,48(2):186−188. doi: 10.3969/j.issn.0517-6611.2020.02.054
[35]	洪佳敏, 林宝妹, 张朝坤, 等. 番石榴醇提物抗氧化能力及α-葡萄糖苷酶抑制活性研究[J]. 西北农林科技大学学报(自然科学版),2019,47(1):139−146. [HONG J M, LIN B M, ZHANG C K, et al. Antioxidant capacity and α-glucosidase inhibitory activity of ethanol extracts from guava fruits[J]. Journal of Northwest A& F University(Nat. Sci. Ed),2019,47(1):139−146.
[36]	欧阳文, 朱晓艾, 刘润南, 等. 番石榴叶具有α-葡萄糖苷酶抑制活性的倍半萜杂二醛分离与鉴定[J]. 湖南中医药大学学报,2014,34(8):17−20. [OU Y W, ZHU X A, LIU R N, et al. Sesquiterpenoids with α-glucosidase inhibitory activities from the leaves of Psidium guajava Linn[J]. Journal of Hunan University of Chinese Medicine,2014,34(8):17−20. doi: 10.3969/j.issn.1674-070X.2014.08.005.017.04