Production of <i>Bacillus subtilis</i> Microbioecologics by Fermentation of Hongqu Residue

ZHANG Chen; WANG Yang; YANG Xin; PAN Dandan; HU Wenlin; SHI Xiangzhu; CHEN Bingtian; LUO Chunlian; NI Li

doi:10.13386/j.issn1002-0306.2021070218

Volume 43 Issue 10

May 2022

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Science and Technology of Food Industry > 2022 > 43(10): 140-148. > DOI: 10.13386/j.issn1002-0306.2021070218

ZHANG Chen, WANG Yang, YANG Xin, et al. Production of Bacillus subtilis Microbioecologics by Fermentation of Hongqu Residue[J]. Science and Technology of Food Industry, 2022, 43(10): 140−148. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021070218.

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Production of Bacillus subtilis Microbioecologics by Fermentation of Hongqu Residue

ZHANG Chen^{1, 2, 3,},
WANG Yang^{1, 2},
YANG Xin^{1, 2},
PAN Dandan^{1, 2},
HU Wenlin⁴,
SHI Xiangzhu⁵,
CHEN Bingtian⁶,
LUO Chunlian⁷,
NI Li^{1, 2, 3, ,}

1.
Institute of Food Science and Technology, Fuzhou University, Fuzhou 350108, China
2.
Fujian Food Biotechnology Innovation Engineering Technology Research Center, Fuzhou 350108, China
3.
Food Nutrition and Health Research Center, Fuzhou University, Jinjiang 362251, China
4.
Guangdong Tianyi Biological Technology Co., Ltd., Zhanjiang 524300, China
5.
Fujian Xinminke Biological Technology Development Co., Ltd., Fuzhou 350008, China
6.
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350012, China
7.
Fujian Industrial Products Production License Review Technology Center, Fuzhou 350013, China

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Received Date: July 19, 2021
Available Online: March 20, 2022

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Abstract

Abstract

In this study, single factor and response surface analysis were used to optimize the conditions for the production of Bacillus subtilis using Hongqu residue, the content of soluble sugar and soluble protein components during the fermentation were determined to analyze the limiting factors of bacterial growth, and the storage stability and the in vitro antioxidant activity of the microbioecologics were determined. The results showed that the highest yield of Bacillus subtilis in microbioecologics was (9.3±0.3) lg CFU/mL that was produced at 37 ℃ for 48 h with the conditions of 100 g/L Hongqu residue, and shaking speed of 220 r/min with 10% inoculation volume of 5 lg CFU/mL Bacillus subtilis. The content of soluble glucose in Hongqu residue was the main factor limiting the growth of Bacillus subtilis. When soluble sugar contents were low, the growth of Bacillus subtilis basically stopped. According to the accelerated preservation experiment, the viable count of probiotics stored at 20 ℃ for three months was 8.7 lg CFU/mL, which was higher than the criteria of microbioecologics application. The microbioecologics had strong antioxidant activity that scavenging capacity of DPPH free radical and hydroxyl free radical were 0.25 mg/mL (IC₅₀) and 0.69 mg/mL (IC₅₀) , respectively. This study proved that Hongqu residue could be used as a raw material to prepare Bacillus subtilis probiotics, which provided a practical basis for the sustainable green processing of microbial fermentation residue.
- Hongqu residue,
- Bacillus subtilis,
- preparation of probiotics,
- storage stability,
- antioxidant activity

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References

[1]	陈旭峰. 红曲霉壳聚糖的制备[D]. 杭州: 浙江工业大学, 2004. CHEN X F. The preparation of chitosan by fermenting Monascus spp[D]. Hangzhou: Zhejiang University of Technology, 2004.
[2]	董晔, 杨姿, 贾梦琦, 等. 红曲废菌渣中有效成分的综合提取工艺[J]. 食品工业科技,2018,39(3):158−166. [DONG Y, YANG Z, JIA M Q, et al. The integrated extraction process of effective ingredients from Monascus mycelium[J]. Science and Technology of Food Industry,2018,39(3):158−166. DONG Y, YANG Z, JIA M Q, et al. The integrated extraction process of effective ingredients from Monascus mycelium[J]. Science and Technology of Food Industry, 2018, 39(3): 158-166.
[3]	邓毛程, 李静, 顾宗珠, 等. 红曲霉菌体蛋白复合酶解的研究[J]. 中国酿造,2011,30(12):148−151. [DENG M C, LI J, GU Z Z, et al. Enzymatic hydrolysis of cell protein of Mononscus[J]. China Brewing,2011,30(12):148−151. doi: 10.3969/j.issn.0254-5071.2011.12.042 DENG M C, LI J, GU Z Z, et al. Enzymatic hydrolysis of cell protein of Mononscus[J]. China Brewing, 2011, 30(12): 148-151. doi: 10.3969/j.issn.0254-5071.2011.12.042
[4]	CHEN W P, FENG Y L, ISTVAN M, et al. Nature and nurture: Confluence of pathway determinism with metabolic and chemical serendipity diversifies Monascus azaphilone pigments[J]. Natural Product Reports,2019,36(4):561−572. doi: 10.1039/C8NP00060C
[5]	黄颖颖, 陈慎, 杨成龙, 等. 红曲色素和桔霉素代谢调控方法的研究进展[J]. 中国调味品,2018,43(2):188−194. [HUANG Y Y, CHEN S, YANG C L, et al. A review on metabolism regulation methods of pigment and citrinin in Monascus[J]. Chinese Condiment,2018,43(2):188−194. doi: 10.3969/j.issn.1000-9973.2018.02.045 HUANG Y Y, CHEN S, YANG C L, et al. A review on metabolism regulation methods of pigment and citrinin in Monascus[J]. Chinese Condiment, 2018, 43(2): 188-194. doi: 10.3969/j.issn.1000-9973.2018.02.045
[6]	戴秉扬. 红曲霉发酵优化及色素提取工艺研究[J]. 广东化工,2019,46(24):50−56. [DAI B Y. Monascus fermentation process optimization and pigment extraction[J]. Guangdong Chemical Industry,2019,46(24):50−56. DAI B Y. Monascus Fermentation process optimization and pigment extraction[J]. Guangdong Chemical Industry, 2019, 46(24): 50-56.
[7]	杨晓辉, 刘利军, 马玉龙. 红曲红色素生产中发酵废渣的综合利用[J]. 粮食与饲料工业,2007(8):30−31. [YANG X H, LIU L J, MA Y L. Comprehensive utilization of fermentation waste residue in production of Monascus red-pigment[J]. Cereal & Feed Industry,2007(8):30−31. doi: 10.3969/j.issn.1003-6202.2007.08.013 YANG X H, LIU L J, MA Y L. Comprehensive utilization of fermentation waste residue in production of Monascus red-pigment[J]. Cereal & Feed Industry, 2007 (8): 30-31. doi: 10.3969/j.issn.1003-6202.2007.08.013
[8]	邢宏博, 曾李, 许赣荣, 等. 红曲菌主要的活性物质及红曲产品在饲料行业应用前景[J]. 广东饲料,2020,29(3):28−31. [XING H B, ZENG L, XU G R, et al. Main active substances of Monascus and application prospect of Monascus products in feed industry[J]. Guangdong Feed,2020,29(3):28−31. doi: 10.3969/j.issn.1005-8613.2020.03.008 XING H B, ZENG L, XU G R, et al. Main active substances of Monascus and application prospect of Monascus products in feed industry[J]. Guangdong Feed, 2020, 29(3): 28-31. doi: 10.3969/j.issn.1005-8613.2020.03.008
[9]	周永红. 饲料原料中霉菌及霉菌毒素对饲料产品适口性的影响[J]. 饲料工业,2005,26(5):51−55. [ZHOU Y H. Effects of molds and mycotoxins in feed materials on palatability of feed products[J]. Feed Industry,2005,26(5):51−55. doi: 10.3969/j.issn.1001-991X.2005.05.015 ZHOU Y H. Effects of molds and mycotoxins in feed materials on palatability of feed products[J]. Feed Industry, 2005, 26(5): 51-55. doi: 10.3969/j.issn.1001-991X.2005.05.015
[10]	吕锋. 袋装混菌发酵全价饲料的研究[D]. 合肥: 安徽农业大学, 2010. LÜ F. The study of mixed fermentation of feed bags full price[D]. Hefei: Anhui Agricultural University, 2010.
[11]	宋立立, 张晨旭, 张连水. 枯草芽孢杆菌在发酵饲料中的应用研究进展[J]. 中国饲料,2021(9):17−21. [SONG L L, ZHANG C X, ZHANG L S. Research progress on application of Bacillus subtilis in fermented feed[J]. China Feed,2021(9):17−21. SONG L L, ZHANG C X, ZHANG L S. Research progress on application of Bacillus subtilis in fermented feed[J]. China Feed, 2021, 00(9): 17-21.
[12]	MARIANA B M, JORL M D, JASONC W, et al. Effect of Bacillus subtilis C-3102 on nursing piglet fecal microflora, fecal consistency and growth performance[J]. Journal of Animal Science,2018,96:112−113.
[13]	LAN R X, KIM I H. Effects of Bacillus licheniformis and Bacillus subtilis complex on growth performance and faecal noxious gas emissions in growing-finishing pigs[J]. Journal of The Science of Food and Agriculture,2019,99(4):1554−1560. doi: 10.1002/jsfa.9333
[14]	ZAINELDIN A I, HEGAZI S, KOSHIO S, et al. Bacillus subtilisas probiotic candidate for red sea bream: Growth performance, oxidative status, and immune response traits[J]. Fish and Shellfish Immunology,2018,79:303−312. doi: 10.1016/j.fsi.2018.05.035
[15]	JEONG J S, KIM I H. Effect of Bacillus subtilis C-3102 spores as a probiotic feed supplement on growth performance, noxious gas emission, and intestinal microflora in broilers[J]. Poultry Science,2014,93(12):3097−3103. doi: 10.3382/ps.2014-04086
[16]	杜晓雨, 赵恺, 赵志敏, 等. 枯草芽孢杆菌微生态制剂发酵研究进展[J]. 微生物学通报,2020,47(3):903−914. [DU X Y, ZHAO K, ZHAO Z M, et al. Progress on Bacillus subtilis microbial ecological agents by fermentation[J]. Microbiology China,2020,47(3):903−914. DU X Y, ZHAO K, ZHAO Z M, et al. Progress on Bacillus subtilis microbial ecological agents by fermentation[J]. Microbiology China, 2020, 47(3): 903-914.
[17]	DI J, CHU Z P, ZHANG S H, et al. Evaluation of the potential probiotic Bacillus subtilis isolated from two ancient sturgeons on growth performance, serum immunity and disease resistance of Acipenser dabryanus[J]. Fish and Shellfish Immunology,2019,93:711−719. doi: 10.1016/j.fsi.2019.08.020
[18]	LECLERE V, BECHET M, ADAM A, et al. Mycosubtilin overproduction by Bacillus subtilis BBG100 enhances the organism’s antagonistic and biocontrol activities[J]. Applied and Environmental Microbiology,2005,71(8):4577−4584. doi: 10.1128/AEM.71.8.4577-4584.2005
[19]	MIN Y N, YANG H L, XU Y X, et al. Effects of dietary supplementation of synbiotics on growth performance, intestinal morphology, sIgA content and antioxidant capacities of broilers[J]. Journal of Animal Physiology and Animal Nutrition,2016,100(6):1073−1080. doi: 10.1111/jpn.12479
[20]	江敏, 胡小军, 王标诗, 等. 发酵法制备马氏珠母贝抗氧化多肽工艺及清除自由基的研究[J]. 食品与发酵科技,2017,53(4):32−38. [JIANG M, HU X J, WANG B S, et al. Fermentation technology and free radical scavenging of pinctada martensii antioxidant polypeptides[J]. Food and Fermentation Sciences & Technology,2017,53(4):32−38. JIANG M, HU X J, WANG B S, et al. Fermentation technology and free radical scavenging of pinctada martensii antioxidant polypeptides[J]. Food and Fermentation Sciences & Technology, 2017, 53(4): 32-38.
[21]	郭利娜, 朱玉, 刁明明, 等. 枯草芽孢杆菌发酵小米糠对其抗氧化肽含量与抗氧化活性的影响[J]. 食品科学,2015,36(13):196−201. [GUO L N, ZHU Y, DIAO M M, et al. Effect of fermentation by Bacillus subtilis on antioxidant peptide content and antioxidant activity of millet bran[J]. Food Science,2015,36(13):196−201. doi: 10.7506/spkx1002-6630-201513036 GUO L N, ZHU Y, DIAO M M, et al. Effect of fermentation by Bacillus subtilis on antioxidant peptide content and antioxidant activity of millet bran[J]. Food Science, 2015, 36(13): 196-201. doi: 10.7506/spkx1002-6630-201513036
[22]	ZHANG Y W, LIU J, LU X, et al. Isolation and identification of an antioxidant peptide prepared from fermented peanut meal using Bacillus subtilis fermentation[J]. International Journal of Food Properties,2014,17(6):1237−1253. doi: 10.1080/10942912.2012.675605
[23]	朱征宇, 汪恩浩, 王媛. 应用统计分析和化学动力学参数评价生物制品的稳定性[J]. 中国生物制品学杂志,2008,21(2):126−130. [ZHU Z Y, WANG E H, WANG Y. Evaluation of stability of biologics by statistic analysis and chemical kinetic parameters[J]. Chinese Journal of Biologicals,2008,21(2):126−130. doi: 10.3969/j.issn.1004-5503.2008.02.012 ZHU Z Y, WANG E H, WANG Y. Evaluation of stability of biologics by statistic analysis and chemical kinetic parameters[J]. Chinese Journal of Biologicals, 2008, 21(2): 126-130. doi: 10.3969/j.issn.1004-5503.2008.02.012
[24]	黄梦姣. 茶渣深加工综合利用及咀嚼片的工艺研究[D]. 西安: 陕西科技大学, 2018. HUANG M J. Comprehensive utilization of tea residue deep processing and process research of chewable tablets[D]. Xi'an: Shaanxi University of Science & Technology, 2018.
[25]	梁红雁, 蒲万霞, 吴润, 等. 活菌制剂的质量控制指标及其研究现状[J]. 黑龙江畜牧兽医,2015(13):79−81. [LIANG H Y, PU W X, WU R, et al. Quality control index of live bacteria preparation and its research status[J]. Heilongjiang Animal Science and Veterinary Medicine,2015(13):79−81. LIANG H Y, PU W X, WU R, et al. Quality control index of live bacteria preparation and its research status[J]. Heilongjiang Animal Science and Veterinary Medicine, 2015, 00(13): 79-81.
[26]	张日俊. 微生态制剂的质量鉴别与选择、检测指标和标准[J]. 饲料工业,2010,31(20):1−4. [ZHANG R J. Quality identification, selection, detection index and standard of microecologics[J]. Feed Industry,2010,31(20):1−4. doi: 10.3969/j.issn.1001-991X.2010.20.001 ZHANG R J. Quality identification, selection, detection index and standard of microecologics[J]. Feed Industry, 2010, 31(20): 1-4. doi: 10.3969/j.issn.1001-991X.2010.20.001
[27]	张亚茹, 张伟涛, 王硕, 等. 枯草芽孢杆菌N-2-10体外益生特性评价[J]. 饲料工业,2020,41(24):12−17. [ZHANG Y R, ZHANG W S, WANG S, et al. Evaluation of in vitro probiotic properties of Bacillus subtilis N2-10[J]. Feed Industry,2020,41(24):12−17. ZHANG Y R, ZHANG W S, Wang S, et al. Evaluation of in vitro probiotic properties of Bacillus subtilis N2-10[J]. Feed Industry, 2020, 41(24): 12-17.
[28]	李燚. 红曲霉废弃物成分分析及应用的研究[D]. 天津: 天津科技大学, 2009. LI Y. The study on the determation of ingredients and the application of Monascus residue[D]. Tianjin: Tianjin University of Science and Technology, 2009.
[29]	陈敏. 虎斑乌贼生殖腺多肽的制备、纯化及其抗氧化活性的研究[D]. 广州: 华南理工大学, 2019. CHEN M. Study on the preparation, purification and antioxidant activity of peptides from cuttlefish (Sepia pharaonis) [D]. Guangzhou: South China University of Technology, 2019.
[30]	李艳艳, 张剑. 表面活性肽的研究进展[J]. 化学通报,2017,80(10):918−924. [LI Y Y, ZHANG J. Research progress in surfactin[J]. Chemistry,2017,80(10):918−924. LI Y Y, ZHANG J. Research progress in surfactin[J]. Chemistry, 2017, 80(10): 918-924.