Composition, Antioxidant Activity and DNA Damage Protective Effect of Bound Phenolics from <i>Ottelia acuminata</i>

LIU Qing; ZHANG Tianyang; HONG Yue; LI Yuanli; LI Wang; GAO Chunyan; LU Yuehong

doi:10.13386/j.issn1002-0306.2021070217

Volume 43 Issue 6

Mar. 2022

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2022 > 43(6): 63-69. > DOI: 10.13386/j.issn1002-0306.2021070217

LIU Qing, ZHANG Tianyang, HONG Yue, et al. Composition, Antioxidant Activity and DNA Damage Protective Effect of Bound Phenolics from Ottelia acuminata[J]. Science and Technology of Food Industry, 2022, 43(6): 63−69. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021070217.

Citation:

PDF (2588 KB)

Composition, Antioxidant Activity and DNA Damage Protective Effect of Bound Phenolics from Ottelia acuminata

LIU Qing^1,,
ZHANG Tianyang¹,
HONG Yue¹,
LI Yuanli¹,
LI Wang¹,
GAO Chunyan^{1, 2},
LU Yuehong^{1, 2, ,}

1.
College of Public Health, Dali University, Dali 671000, China
2.
College of Biological Science and Engineering, North Minzu University, Yinchuan 750021, China

More Information

Received Date: July 19, 2021
Available Online: February 11, 2022

Graphical Abstract

Abstract

Abstract

Folin-Ciocalteu method and sodium nitrite-aluminum chloride method were used to determine total phenolic content and flavonoid content of bound phenolics from the inflorescences, peduncles and leaves of Ottelia acuminata (O. acuminata), respectively. The composition of bound phenolics was analyzed by high performance liquid chromatography (HPLC). The antioxidant activity and protective effect against ROO· mediated DNA damage were also investigated. The results showed that total phenolic content of O. acuminata was in the range of 209.72~366.35 mg GAE/g Extract while total flavonoid content was in the range of 156.69~466.08 mg CAE/g Extract. Five phenolics, namely, caffeic acid, quercetin-3-O-glucoside, luteolin, chlorogenic acid and ferulic acid were identified in bound phenolics of O. acuminata. Trolox equivalent antioxidant activity (TEAC), 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) radical scavenging ability, iron ion reduction antioxidant ability (FRAP) and hydroxyl radical (·OH) scavenging rate IC₅₀ value of different plant parts were in the range of 1.19~1.25 μmol TE/g Extract, 0.74~0.94 mmol/g Extract, 3.61~4.75 mmol Fe²⁺/g Extract, 0.34~1.26 mg/mL, respectively, among which the leaves displayed the strongest antioxidant activity. In the concentration range of 12.5~500 mg/mL, the percentage of double helix DNA in bound phenolics of inflorescences, peduncles and leaves ranged from 22.74% to 50.07%, 18.25% to 53.28% and 25.93% to 53.60%, respectively, among which the leaves showed the strongest DNA damage protective effect, followed by the peduncles. The results showed that O. acuminata was rich in bound phenolics which possessed strong antioxidant activity and DNA damage protective effect.
- O. acuminata,
- bound phenolics,
- antioxidant activity,
- DNA damage

FullText(HTML)

References (41)

References

[1]	严东莹, 李艳, 孙文礼, 等. 基于流式细胞术的海菜花属植物基因组大小测定[J]. 植物科学学报,2020,38(2):173−180. [YAN D Y, LI Y, SUN W L, et al. Estimation of genome sizes of plants from Ottelia Pers. based on flow cytometry[J]. Journal of Plant Science,2020,38(2):173−180.
[2]	汪松, 解焱. 中国物种红色名录[M]. 北京: 高等教育出版社, 2004. WANG S, JIE Y. Red list of Chinese species[M]. Beijing: Higher Education Press, 2004.
[3]	CHEN J M, DU Z Y, LONG Z C, et al. Molecular divergence among varieties of Ottelia acuminata (Hydrocharitaceae) in the Yunnan-Guizhou Plateau[J]. Aquatic Botany,2017,140(5):62−68.
[4]	VYAS S C, DASR R. Protein content of some hydrophytes[J]. Aquatic Botany,1978,5:207−208. doi: 10.1016/0304-3770(78)90062-1
[5]	李原, 杨君兴, 崔桂华, 等. 海菜花营养成分初步分析[J]. 营养学报,2009,31(1):96−97. [LI Y, YANG J X, CUI G H, et al. Analysis of the nutritional composition of Ottelia acuminata(Gagnep.) dandy (hydrocharitaceae)[J]. Journal of Nutrition,2009,31(1):96−97.
[6]	朱静, 杨亚维, 郭爱伟, 等. 云南几大湖泊海菜花营养成分分析[J]. 安徽农业科学,2010,38(24):12952−12953. [ZHU J, YANG Y W, GUO A W, et al. Analysis on nutrient components in Ottelia acuminata several lakes of Yunnan[J]. Anhui Agricultural Sciences,2010,38(24):12952−12953.
[7]	LU Y H, TIAN C R, GAO C Y, et al. Phenolic profile, antioxidant and enzyme inhibitory activities of Ottelia acuminata, an endemic plant from southwestern China[J]. Industrial Crops & Products,2019:138.
[8]	国家中医药管理局. 中华本草[M]. 上海: 上海科学技术出版社, 1999. National Administration of Traditional Chinese Medicine. Chinese herbal medicine[M]. Shanghai: Shanghai Science and Technology Press, 1999.
[9]	LI D, YE B B, HOU Z Y, et al. Long-term performance and microbial distribution of a filed-scale storing multi-pond constructed wetland with Ottelia acuminata for the treatment of non-point source pollution[J]. Journal of Cleaner Production,2020,262:121367.1−121367.9.
[10]	FAN X R, NJERI H K, LI W, et al. Abundant historical gene flow within and among river systems for populations of Ottelia acuminata var. jingxiensis, an endangered macrophyte from southwest China[J]. Aquatic Botany,2019:157.
[11]	SHARIFA SOPLAH S A, ANISS N M. Quantification of polyphenols and antioxidant activity in several herbal and green tea products in Malaysia[C]. Materials Today: Proceedings, 2021, 31(3).
[12]	ALBERTO B E, LAURA M P, FELIPE J A, et al. Anti-inflammatory effect of polyphenols from Chilean currants(Ribes magellanicum and R. punctatum) after in vitro gastrointestinal digestion on Caco-2 cells: Anti-inflammatory activity of in vitro digested Chilean currants[J]. Journal of Functional Foods,2019,59:329−336. doi: 10.1016/j.jff.2019.06.007
[13]	ZHANG T, QIU F, CHEN L, et al. Identification and in vitro anti-inflammatory activity of different forms of phenolic compounds in Camellia oleifera oil[J]. Food Chemistry,2020,344(49):128660.
[14]	LU C R, LI C, CHEN B, et al. Composition and antioxidant, antibacterial, and anti-HepG2 cell activities of polyphenols from seed coat of Amygdalus pedunculata pall[J]. Food Chemistry,2018,265:111−119. doi: 10.1016/j.foodchem.2018.05.091
[15]	ABDELGAWAD A F, MOHAMED A E R, ABEER Y I, et al. A sulfated polyphenols-rich extract from Sabal yapa exhibits antitumor activities in ehrlich ascites carcinoma[J]. Saudi Journal of Biological Sciences,2021,28(5):3117−3125. doi: 10.1016/j.sjbs.2021.02.056
[16]	CHAITI M, SWATI C. Study of dietary polyphenols from natural herbal sources for providing protection against human degenerative disorders[J]. Biocatalysis and Agricultural Biotechnology,2021,33:101956. doi: 10.1016/j.bcab.2021.101956
[17]	MASOMEH M, NAFISEH K, FOROUGH A, et al. Stabilization of telomere by the antioxidant property of polyphenols: Anti-aging potential[J]. Life Sciences,2020,259:118341. doi: 10.1016/j.lfs.2020.118341
[18]	S MITHUL A, SANTAD W, RONG T, et al. Role of dietary polyphenols on gut microbiota, their metabolites and health benefits[J]. Food Research International,2021,142:110189. doi: 10.1016/j.foodres.2021.110189
[19]	詹瑰然, 卯玲, 金林, 等. 滇池金线鲃-海菜花共生生态养殖试验[J]. 农村实用技术,2021(3):54−55. [ZHAN M R, LING L, JIN L, et al. Sinocyclocheilus grahami-Ottelia acuminata (Gagnep.) dandy symbiosis ecological farming experiment[J]. Rural Practical Technology,2021(3):54−55.
[20]	LI Z Z, LEHTTONEN S, MARTINS K, et al. Phylogenomics of the aquatic plant genus Ottelia(Hydrocharitaceae): Implications for historical biogeography[J]. Molecular Phylogenetics and Evolution,2020,152:106939. doi: 10.1016/j.ympev.2020.106939
[21]	LI Z Z, LU M X, ANDREW W G, et al. Genetic diversity and population structure of Ottelia acuminata var. jingxiensis, an endangered endemic aquatic plant from southwest China[J]. Aquatic Botany,2018,152:20−26.
[22]	ZHAI S H, YIN G S, YANG X H. Population genetics of the endangered and wild edible plant Ottelia acuminata in southwestern China using novel SSR markers[J]. Biochemical Genetics,2018,56(3):235−254. doi: 10.1007/s10528-018-9840-2
[23]	ZHAO S T, ZHANG R F, LIU Y, et al. The effect of storage condition on seed germination of six Hydrocharitaceae and Potamogetonaceae species[J]. Aquatic Botany,2017,143:49−53. doi: 10.1016/j.aquabot.2017.09.005
[24]	ZHAO F B, ZHANG W, LIU Y H, et al. Responses of growth and photosynthetic fluorescent characteristics in Ottelia acuminata to a water-depth gradient[J]. Journal of Freshwater Ecology,2018,33(1):285−297. doi: 10.1080/02705060.2018.1443841
[25]	CHEN S Q, CHU Z S. Study on the ammonia tolerance threshold of Ottelia acuminata (Gagnep.) Dandy Seedling[J]. Vegetos-An International Journal of Plant Research,2015,28(2):72−81. doi: 10.5958/2229-4473.2015.00040.3
[26]	CHEN S Q, CHU Z S, ZHOU Y Q, et al. Screening optimal substrates from Erhai lakeside for Ottelia acuminata (Gagnep.) Dandy, an endangered submerged macrophyte in China[J]. Environmental Science and Pollution Research,2018,25(20):19887−19897. doi: 10.1007/s11356-018-2200-2
[27]	郭琦, 高春燕. 硬毛地笋酚类化合物对DNA损伤的保护作用[J]. 食品科学,2018,39(23):54−59. [GUO Q, GAO C Y. Protective effect of phenolic compounds from Lycopus lucidus Turcz. on DNA Damage[J]. Food Science,2018,39(23):54−59.
[28]	CAI R R, ZHOU Z D, CHAI Q Q. Study on the content of total flavonoids, total phenolic acid and antioxidant activity of extracts from Potamogeton crispus L[J]. Jiyinzuxue Yu Yingyong Shengwuxue (Genomics and Applied Biology),2020,39(3):1457−1462.
[29]	乌仁格格. 沙芥不同部位总黄酮含量比较研究[J]. 黑龙江科学,2020,11(14):7−8,11. [URENGUE. Comparative study on total flavonoids content in different parts of Pugionium cornutum[J]. Heilongjiang Science,2020,11(14):7−8,11.
[30]	TAN L, ZHENG X Y, WANG J S, et al. Antioxidant activity and inhibitory activity of ethanol extract from Ficus auriculata fruits against α-glucosidase and acetylcholine esterase[J]. Food Science,2016,37(13):77−81.
[31]	SONG W, YUAN Y, YU N X, et al. Antioxidant capacity of extract from Jiang Tang Xiao Zhi recipe in vitro[J]. Journal of Traditional Chinese Medicine,2020,40(3):393−400.
[32]	张天阳, 陈友霞, 刘珍珍, 等. 不同可食阶段蚕豆结合酚抗氧化活性的研究[J]. 食品工业科技,2021,42(4):39−43. [ZHANG T Y, CHEN Y X, LIU Z Z, et al. Antioxidant activity of bound phenolics from broad beans at different edible stages[J]. Science and Technology of Food Industry,2021,42(4):39−43.
[33]	卢跃红, 田呈瑞, 高春燕. 黄参籽可溶性酯结合酚和糖苷结合酚的组成及其抗氧化活性[J]. 食品工业科技,2016,37(8):158−162. [LU Y H, TIAN X R, GAO C Y. Phenolic composition and antioxidant activity of soluble ester-bound and glycoside-bound phenolics from Sphallerocarpus gracilis seeds[J]. Science and Technology of Food Industry,2016,37(8):158−162.
[34]	林琳, 卢跃红, 陈友霞, 等. 蚕豆多酚对过氧自由基介导的DNA损伤的保护作用[J]. 食品科学,2020,41(17):83−88. [LIN L, LU Y H, CHEN Y X, et al. Protective effect of polyphenols from broad bean on peroxy radical-induced DNA damage[J]. Food Science,2020,41(17):83−88.
[35]	WU S T, SHEN T Y, WANG R H, et al. Phenolic profiles and antioxidant activities of free, esterified and bound phenolic compounds in walnut kernel[J]. Food Chemistry,2021,350:129217. doi: 10.1016/j.foodchem.2021.129217
[36]	JAADAN H, MUSTAPHA A, ABDELMAJID M, et al. Phytochemical screening, polyphenols, flavonoids and tannin content, antioxidant activities and FTIR characterization of Marrubium vulgare L from 2 different localities of northeast of Morocco[J]. Heliyon,2020,6(11):5609.
[37]	K BAJPAII V, ALAM M B, JU M K, et al. Antioxidant mechanism of polyphenol-rich Nymphaea nouchali leaf extract protecting DNA damage and attenuating oxidative stress-induced cell death via Nrf2-mediated heme-oxygenase-1 induction coupled with ERK/p38 signaling pathway[J]. Biomedicine & Pharmacotherapy,2018,103:1397−1407.
[38]	GAO C Y, LU Y H, TIAN C R, et al. Main nutrients, phenolics, antioxidant activity, DNA damage protective effect and microstructure of Sphallerocarpus gracilis root at different harvest time[J]. Food Chemistry,2011,127(2):615−622. doi: 10.1016/j.foodchem.2011.01.053
[39]	ANDERSON R F, FISHER L J, HARA Y, et al. Green tea catechins partially protect DNA from ·OH radical-induced strand breaks and base damage through fast chemical repair of DNA radicals[J]. Carcinogenesis,2001,22(8):1189−1193. doi: 10.1093/carcin/22.8.1189
[40]	王长松, 田莉莉, 张俊刚, 等. 五种中药酚酸类化合物体外抗DNA损伤的作用[J]. 中国药理学与毒理学杂志,2012,26(4):529−533. [WANG C S, TIAN L L, ZHANG J G, et al. Protective effect of five natural phenolic acids against DNA oxidative damage in vitro[J]. Chinese Journal of Pharmacology and Toxicology,2012,26(4):529−533.
[41]	SHUNSUKE Y, HIROYUKI S, HITOMI T, et al. Quercetin-3-O-glucronide inhibits noradrenaline binding to α2-adrenergic receptor, thus suppressing DNA damage induced by treatment with 4-hydroxyestradiol and noradrenaline in MCF-10A cells[J]. The Journal of Steroid Biochemistry and Molecular Biology,2014,143:122−129. doi: 10.1016/j.jsbmb.2014.02.014