Study on Ultrasonic-Assisted Enzymatic Hydrolysis of Distiller’s Grains Cellulose

KE Qiaomei; ZENG Wei; SHUAI Yutong; JIN Jian

doi:10.13386/j.issn1002-0306.2021070206

Volume 43 Issue 8

Apr. 2022

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Science and Technology of Food Industry > 2022 > 43(8): 196-203. > DOI: 10.13386/j.issn1002-0306.2021070206

KE Qiaomei, ZENG Wei, SHUAI Yutong, et al. Study on Ultrasonic-Assisted Enzymatic Hydrolysis of Distiller’s Grains Cellulose[J]. Science and Technology of Food Industry, 2022, 43(8): 196−203. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021070206.

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Study on Ultrasonic-Assisted Enzymatic Hydrolysis of Distiller’s Grains Cellulose

KE Qiaomei^1,,
ZENG Wei¹,
SHUAI Yutong¹,
JIN Jian^{1, 2, ,}

1.
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
2.
Solid-state Fermentation Resource Utilization Key Laboratory of Sichuan Province, Yibin 644000, China

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Received Date: July 18, 2021
Available Online: February 16, 2022

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Abstract

Abstract

In order to promote the development and utilization of distiller’s grain resources in China, the present study explored the enzymatic hydrolysis conditions of distiller’s grains cellulose based on ultrasonic pretreatment. Firstly, the effects of ultrasound parameters (time, temperature, power) and enzymatic hydrolysis process parameters (time, pH, temperature, enzyme addition, substrate concentration) on the enzymatic hydrolysis of vinasse cellulose were studied through single-factor experiments. The Plackett-Burman test was carried out to screen out the key factors which affected the enzymatic hydrolysis reaction, and then the Box-Behnken test was used to optimize the enzymatic hydrolysis process. The Plackett-Burman test results showed that the key factors affecting the enzymatic hydrolysis of vinasse cellulose were ultrasound time, enzymatic hydrolysis time, enzymatic hydrolysis pH, and enzyme addition. The Box-Behnken results showed that the optimal enzymolysis process conditions were ultrasonic time 21 min, enzymolysis time 140 min, enzymatic hydrolysis pH5.41, enzyme addition 990 U/g, ultrasonic temperature 60 ℃, ultrasonic power 200 W, enzymolysis temperature 50 ℃, and the concentration of enzymatic hydrolysis substrate 1:20 g/mL. Under these conditions, the cellulose conversion rate of distiller’s grains were 5.62%, which was 35.4% higher than that of the control group. The results showed that ultrasonic pretreatment was an effective way to improve the cellulose conversion rate of distiller’s grains.
- distiller's grains,
- cellulose,
- enzymatic hydrolysis,
- ultrasound,
- response surface

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