DU Jinjie, WU Xinhui, DAI Yuqing, et al. Quality Component Analysis and Antioxidant Function of Theabrownins from Liupao Tea during Fermentation Process[J]. Science and Technology of Food Industry, 2022, 43(6): 50−55. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021070198.
Citation: DU Jinjie, WU Xinhui, DAI Yuqing, et al. Quality Component Analysis and Antioxidant Function of Theabrownins from Liupao Tea during Fermentation Process[J]. Science and Technology of Food Industry, 2022, 43(6): 50−55. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021070198.

Quality Component Analysis and Antioxidant Function of Theabrownins from Liupao Tea during Fermentation Process

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  • Received Date: July 18, 2021
  • Available Online: January 06, 2022
  • This study investigated the changes of the sensory qualities of appearance, color, aroma, taste, leaf bottom and the chemical components of tea polyphenols, free amino acids, soluble sugar, caffeine, flavonoids, theabrownin (TB), theaflavin (TF) and thearubigin (TR) using the Liupao tea during processing and was produced by group species Zhongcha Tea Factory in Cangwu County, Wuzhou City, Guangxi, China. In addition, the chemical composition, and the antioxidant of the theabrownin (TB) that extracted from the Liupao tea were investigated. The results showed that the contents of water extract, tea polyphenols, thearubigin (TR), free amino acids and flavonoids were significantly decreased during the fermentation process (P<0.05), while the content of TB was significantly increased (P<0.05). The taste of tea had changed from strong and irritant to mellow and smooth. With the increasing of fermentation days, the contents of total sugar, total protein and total phenol in theabrownins all showed an increasing trend. In vitro antioxidant assays showed that the half maximal inhibitory concentration (IC50) of each group were 10.09 μg/mL (VC), 69.33 μg/mL (20 d), 49.95 μg/mL (40 d) and 51.85 μg/mL (60 d). The scavenging activity of DPPH free radical was as follows: VC>40 d>60 d>20 d. The results of this study would provide a theoretical basis for the production and processing of Liupao tea.
  • [1]
    石荣强, 温立香, 曾玉凤, 等. 六堡茶品质研究进展[J]. 中国茶叶加工,2020(2):43−47. [SHI R Q, WEN L X, ZENG Y F, et al. Research progress on quality of Liupao tea[J]. China Tea Processing,2020(2):43−47.
    [2]
    马婉君, 马士成, 刘春梅, 等. 六堡茶的化学成分及生物活性研究进展[J]. 茶叶科学,2020,40(3):289−304. [MA W J, MA S C, LIU C M, et al. Research progress on chemical composition and biological activity of Liupao tea[J]. Journal of Tea Science,2020,40(3):289−304. doi: 10.3969/j.issn.1000-369X.2020.03.001
    [3]
    曹中环, 邱瑞瑾, 王登良, 等. 六堡茶加工过程中主要生化成分的变化[J]. 广东茶业,2016(3):26−28. [CAO Z H, QIU R J, WANG D N, et al. Changes of main biochemical components in Liubao tea during processing[J]. Guangdong Tea Industry,2016(3):26−28. doi: 10.3969/j.issn.1672-7398.2016.03.008
    [4]
    刘家奇, 邵宛芳, 赵宝权, 等. 普洱茶(熟茶)茶粉、黑茶茶粉、六堡茶减肥作用的研究[J]. 中华中医药杂志,2014,29(1):108−112. [LIU J Q, SHAO W F, ZHAO B Q, et al. Study of fermented pu-erh tea powder, dark tea powder and liupu tea on losing weight in hyperlipidemia model rats[J]. China Journal of Traditional Chinese Medicine and Pharmacy,2014,29(1):108−112.
    [5]
    侯粲, 肖杰, 王黎明, 等. 六堡茶改善痰湿质功效评价及基于肠道菌群调节的祛湿机制研究[J]. 食品工业科技,2021:1−14. [HOU C, XIAO J, WANG L M, et al. Effect of Liupao tea in regulating phlegm-dampness constitution and mechanism on intestinal microbiota[J]. Science and Technology of Food Industry,2021:1−14.
    [6]
    ZHU J, WU M, ZHOU H, et al. Liubao brick tea activates the PI3K-Akt signaling pathway to lower blood glucose, metabolic disorders and insulin resistance via altering the intestinal flora[J]. Food Research International,2021,148:110594. doi: 10.1016/j.foodres.2021.110594
    [7]
    GONG Z P, OUYANG J, WU X L, et al. Dark tea extracts: Chemical constituents and modulatory effect on gastrointestinal function[J]. Biomed Pharmacother,2020,130:110514. doi: 10.1016/j.biopha.2020.110514
    [8]
    范艺凡, 彭春秀, 冯励, 等. 不同分子质量茶褐素对高脂饮食大鼠脂质代谢关键酶的影响[J]. 中国食品学报,2017,17(11):9−19. [FAN Y F, PENG C X, FENG L, et al. Effect of different TB frictions on the key-enzyme of lipid metabolite in high-lipid diet rats[J]. Journal of Chinese Institute of Food Science and Technology,2017,17(11):9−19.
    [9]
    龚受基, 滕翠琴, 梁东姨, 等. 六堡茶茶褐素体外降脂功效研究[J]. 茶叶科学,2020,40(4):536−543. [GONG S J, TENG C Q, LIANG D Y, et al. In vitro study on hypolipidemic effects of theabrownins in Liupao tea[J]. Journal of Tea Science,2020,40(4):536−543. doi: 10.3969/j.issn.1000-369X.2020.04.012
    [10]
    HUANG F, ZHENG X, MA X, et al. Theabrownin from Pu-erh tea attenuates hypercholesterolemia via modulation of gut microbiota and bile acid metabolism[J]. Nature Communications,2019,10(1):4971. doi: 10.1038/s41467-019-12896-x
    [11]
    王天禄. 普洱茶茶褐素的分离、抗氧化与体外抑菌研究[D]. 天津: 天津科技大学, 2016.

    WANG T L. Study on Isolation, antioxidant activity and antimicrobial activity of theabrownins from Pu-erh tea[D]. Tianjin: Tianjin Tianjin University of Science and Technology, 2016.
    [12]
    SHOUJI G. The influence of Liupao tea theabrownins on the profile of gut microbiota in mice[J]. Hans Journal of Food and Nutrition Science,2020,9(1):101−107. doi: 10.12677/HJFNS.2020.91013
    [13]
    何英姿, 逯钊琦, 陈艳芳, 等. 六堡茶茶褐素的提取工艺及其抗氧化活性研究[J]. 安徽农业科学,2012,40(35):17301−17303. [HE Y Z, LU Z Q, CHEN Y F, et al. Extraction technology of theabrownine from Liubao tea and its antioxidant activity research[J]. Journal of Anhui Agricultural Sciences,2012,40(35):17301−17303. doi: 10.3969/j.issn.0517-6611.2012.35.113
    [14]
    JIN W, ZHOU L, YAN B, et al. Theabrownin triggers DNA damage to suppress human osteosarcoma U2OS cells by activating p53 signalling pathway[J]. Journal of Cellular and Molecular Medicine,2018,22(9):4423−4436. doi: 10.1111/jcmm.13742
    [15]
    刘泽森, 温立香, 何梅珍, 等. 不同外形、年份六堡茶品质变化分析[J]. 热带农业科学,2016,36(11):81−86. [LIU Z S, WEN L X, HE M Z, et al. Quality change comparison of different shapes and years of Liupao tea[J]. Chinese Journal of Tropical Agriculture,2016,36(11):81−86.
    [16]
    刘泽森, 温立香, 何梅珍, 等. 不同发酵方式对六堡茶品质的影响研究[J]. 安徽农业科学,2016,44(18):91−92. [LIU Z S, WEN L X, HE M Z, et al. Effects of different fermentation modes on quality of Liupao tea[J]. Journal of Anhui Agricultural Sciences,2016,44(18):91−92. doi: 10.3969/j.issn.0517-6611.2016.18.029
    [17]
    JIASHUN GONG C P T C. Effects of theabrownin from Pu-erh tea on the metabolism of serum lipids in rats: Mechanism of action[J]. Journal of Food Science,2010,6(75):182.
    [18]
    甘甜, 邓岳, 聂远洋, 等. 雅安藏茶贮藏过程中滋味和风味成分的变化[J]. 中国测试,2017,43(1):50−54. [GAN T, DENG Y, NIE Y Y, et al. Changes of taste and flavor compounds in Ya'an Tibetan tea during storage[J]. China Measurement & Test,2017,43(1):50−54. doi: 10.11857/j.issn.1674-5124.2017.01.011
    [19]
    张云天, 姚晓玲, 鲁江, 等. 黑茶茶褐素的研究现状及进展[J]. 食品工业科技,2017,38(11):395−399. [ZHANG Y T, YAO X L, LU J, et al. Current research status and progress of the theabrownine in dark tea[J]. Science and Technology of Food Industry,2017,38(11):395−399.
    [20]
    曾贞, 罗军武, 杨阳, 等. 微波固样方法研究与应用[J]. 茶叶通讯,2007(1):4−6. [ZHEN C, LUO J W, YANG Y, et al. Study and application of the microwave technique to tea sample[J]. Journal of Tea Communication,2007(1):4−6. doi: 10.3969/j.issn.1009-525X.2007.01.005
    [21]
    黄意欢. 茶学实验技术[M]. 北京: 中国农业出版社, 1995.

    HUANG Y H. Experimental techniques of tea science[M]. Beijing: China Agriculture Press, 1995.
    [22]
    周婷, 黄文权, 谢加仕, 等. 六堡茶茶褐素的提取工艺优化及其理化性质[J]. 食品工业科技,2018,39(18):143−148. [ZHOU T, HUANG W Q, XIE J S, et al. Optimization of the extraction technology and the physiochemical properties of theabrownin from Liupao tea[J]. Science and Technology of Food Industry,2018,39(18):143−148.
    [23]
    田燕华. 液态发酵生产茶褐素及其生物活性研究[D]. 武汉: 华中科技大学, 2019.

    TIAN Y H. Study on the production of theabrownins by submerged fermentation and its biological activity[D]. Wuhan: Huazhong University of Science and Technology, 2019.
    [24]
    LIN F, WEI X, LIU H, et al. State-of-the-art review of dark tea: From chemistry to health benefits[J]. Trends in Food Science & Technology,2021,109:126−138.
    [25]
    郑淑娟, 盛耀, 欧小群, 等. 渥堆黑茶香气和主要功效研究进展[J]. 食品工业科技,2016,37(20):366−370. [ZHEN S J, SHENG Y, OU X Q, et al. Research progress in aroma and main effects of pile fermentation Chinese dark tea[J]. Science and Technology of Food Industry,2016,37(20):366−370.
    [26]
    张芬, 温立香, 彭靖茹, 等. 六堡茶渥堆过程中主要生化成分和茶汤色泽变化及其相关性研究[J]. 食品科技,2019,44(2):83−87. [ZHANG F, WEN L X, PENG J R, et al. The changes and correlations of main chemical components and color of tea infusion during pile-fermentation of Liupao tea[J]. Food Science and Technology,2019,44(2):83−87.
    [27]
    方开星, 姜晓辉, 秦丹丹, 等. 高氨基酸和高茶氨酸茶树资源筛选[J]. 核农学报,2019,33(9):1724−1733. [FANG K X, JIANG X H, QIN D D, et al. Selection of tea germplasm with high contents of amino acid and theanine[J]. Journal of Nuclear Agricultural Sciences,2019,33(9):1724−1733. doi: 10.11869/j.issn.100-8551.2019.09.1724
    [28]
    ZHU M, LI N, ZHOU F, et al. Microbial bioconversion of the chemical components in dark tea[J]. Food Chemistry,2020,312:126043. doi: 10.1016/j.foodchem.2019.126043
    [29]
    ZHAO M, SU X Q, NIAN B, et al. Integrated meta-omics approaches to understand the microbiome of spontaneous fermentation of traditional chinese Pu-erh tea[J]. mSystems,2019,4(6):e00680−19.
    [30]
    LIU S, HUANG H. Assessments of antioxidant effect of black tea extract and its rationals by erythrocyte haemolysis assay, plasma oxidation assay and cellular antioxidant activity (CAA) assay[J]. Journal of Functional Foods,2015,18:1095−1105. doi: 10.1016/j.jff.2014.08.023
    [31]
    孙海燕. 贺兰山东麓干红葡萄酒多酚组分与其抗氧化、抗癌活性的关联性研究[D]. 咸阳: 西北农林科技大学, 2019.

    SUN H Y. Study on antioxidant and anticancer activity of polyphenols in dry red wines from eastern foot of helan mountain in Ningxia[D]. Xianyang: Northwest A&F University, 2019.
    [32]
    陈荣义. 茶多酚的提取纯化及其改性的研究[D]. 成都: 四川大学, 2005.

    CHE R Y. Preparation and modification research of tea polyphenols[D]. Chengdu: Sichuan University, 2005.
    [33]
    SANDIP P D G C S. Total polyphenol content, antioxidant activity and lipid peroxidation inhibition efficacy of branded tea (Camellia sinensis) available in India[J]. International Journal of Tea Science,2012,3(8):13−20.
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