Ameliorative Effect and Mechanism of Probiotics Combined with Resveratrol on Chronic Alcoholic Liver Injury in Mice

WANG Yin; ZHANG Lanwei

doi:10.13386/j.issn1002-0306.2021070170

Volume 43 Issue 5

Feb. 2022

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2022 > 43(5): 374-380. > DOI: 10.13386/j.issn1002-0306.2021070170

WANG Yin, ZHANG Lanwei. Ameliorative Effect and Mechanism of Probiotics Combined with Resveratrol on Chronic Alcoholic Liver Injury in Mice[J]. Science and Technology of Food Industry, 2022, 43(5): 374−380. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021070170.

Citation:

PDF (3368 KB)

Ameliorative Effect and Mechanism of Probiotics Combined with Resveratrol on Chronic Alcoholic Liver Injury in Mice

WANG Yin,
ZHANG Lanwei^,

College of Food Science and Engineering, Ocean University of China, Qingdao 266000, China

More Information

Received Date: July 18, 2021
Accepted Date: December 21, 2021
Available Online: December 27, 2021

Graphical Abstract

Abstract

Abstract

To explore the protective effect and possible mechanism of probiotics combined with resveratrol on chronic alcoholic liver injury, C57BL/6J mice were used to construct chronic alcoholic liver injury model. Mice were randomly divided into the control group (Con group), themodel group (Mod group), three intervention groups of probiotics combined with resveratrol (Res) (Lactobacillus paracasei J5+Res (J5+Res), Lactobacillus casei YRL577+Res (YRL577+Res), Bifidobacterium animalis F1-7+Res (F1-7+Res)) groups, and the positive drug tiopronin group (LP group). After the experiment, the liver lipid content, alcohol metabolism enzyme activities, oxidative stress level and other indexes of mice were investigated. In order to further explore the mechanism of the combined action, the mRNA expressions of oxidative stress related genes CYP2E1, nuclear factor erythroid-2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) in liver were analyzed. The results showed that compared with Mod group, probiotics combined with resveratrol could significantly reduce the contents of triglyceride, total cholesterol, serum aspartate aminotransferase and alanine aminotransferase activites in liver of mice (P<0.05), increase the activities of alcohol metabolism enzymes alcohol dehydrogenase and acetaldehyde dehydrogenase in liver, inhibit the activity of CYP2E1 and its mRNA expression in liver. In addition, the contents of glutathione as well as the activities of superoxide dismutase and catalase in liver significantly increased (P<0.05), the Nrf2/HO-1 pathway was effectively activated compared with Mod group (P<0.05). Nrf2 mRNA expression was up-regulated by 2.6, 3.7 and 2.7 times in J5+Res, YRL577+Res and F1-7+Res groups, and HO-1 was up-regulated by 2.0, 6.2 and 4.0 times, respectively. Therefore, probiotics combined with resveratrol mayprevente chronic alcoholic liver injury through the Nrf2/HO-1 pathway.
- probiotics,
- resveratrol,
- alcoholic liver injury,
- oxidative stress

FullText(HTML)

References (30)

References

[1]	REHM J, MATHERS C, POPOVA S, et al. Alcohol and global health global burden of disease and injury and economic cost attributable to alcohol use and alcohol-use disorders[J]. Lancet,2009,373(9682):2223−2233. doi: 10.1016/S0140-6736(09)60746-7
[2]	LIEBER C S. Alcoholic fatty liver: Its pathogenesis and mechanism of progression to inflammation and fibrosis[J]. Alcohol,2004,34(1):9−19. doi: 10.1016/j.alcohol.2004.07.008
[3]	CHENG C F, PAN T M. Protective effect of monascus-fermented red mold rice against alcoholic liver disease by attenuating oxidative stress and inflammatory response[J]. Journal of Agricultural and Food Chemistry,2011,59(18):9950−9957. doi: 10.1021/jf202577t
[4]	LOUVET A, MATHURIN P. Alcoholic liver disease: Mechanisms of injury and targeted treatment[J]. Nature Reviews Gastroenterology & Hepatology,2015,12(4):231−242.
[5]	ZHU J J, REN T T, ZHOU M Y, et al. The combination of blueberry juice and probiotics reduces apoptosis of alcoholic fatty liver of mice by affecting SIRT1 pathway[J]. Drug Design Development and Therapy,2016,10:1649−1661.
[6]	TIAN F W, CHI F F, WANG G, et al. Lactobacillus rhamnosus CCFM1107 treatment ameliorates alcohol-induced liver injury in a mouse model of chronic alcohol feeding[J]. Journal of Microbiology,2015,53(12):856−863. doi: 10.1007/s12275-015-5239-5
[7]	KASDALLAH-GRISSA A, MORNAGUI B, AOUANI E, et al. Resveratrol, a red wine polyphenol, attenuates ethanol-induced oxidative stress in rat liver[J]. Life Sciences,2007,80(11):1033−1039. doi: 10.1016/j.lfs.2006.11.044
[8]	NAM Y, KIM J H, KONKIT M, et al. Hepatoprotective effects of Lactococcus chungangensis CAU 1447 in alcoholic liver disease[J]. Journal of Dairy Science,2019,102(12):10737−10747. doi: 10.3168/jds.2019-16891
[9]	PETRELLA C, CARITO V, CARERE C, et al. Oxidative stress inhibition by resveratrol in alcohol-dependent mice[J]. Nutrition,2020,79−80:110783. doi: 10.1016/j.nut.2020.110783
[10]	LIANG X, ZHANG Z, ZHOU X H, et al. Probiotics improved hyperlipidemia in mice induced by a high cholesterol diet via downregulating FXR[J]. Food & Function,2020,11(11):9903−9911.
[11]	ZHANG Z, ZHOU H, GUAN M Y, et al. Lactobacillus casei YRL577 combined with plant extracts reduce markers of non-alcoholic fatty liver disease in mice[J]. British Journal of Nutrition,2021,125(10):1081−1091. doi: 10.1017/S0007114520003013
[12]	ZHANG Z, ZHOU H, ZHOU X H, et al. Lactobacillus casei YRL577 ameliorates markers of non-alcoholic fatty liver and alters expression of genes within the intestinal bile acid pathway[J]. British Journal of Nutrition,2021,125(5):521−529. doi: 10.1017/S0007114520003001
[13]	GU Z, WU Y, WANG Y, et al. Lactobacillus rhamnosusgranules dose-dependently balance intestinal microbiome disorders and ameliorate chronic alcohol-induced liver injury[J]. Journal of Medicinal Food,2020,23(2):114−124. doi: 10.1089/jmf.2018.4357
[14]	ZHANG M, WANG C, WANG C, et al. Enhanced AMPK phosphorylation contributes to the beneficial effects of Lactobacillus rhamnosus GG supernatant on chronic-alcohol-induced fatty liver disease[J]. Journal of Nutritional Biochemistry,2015,26(4):337−44. doi: 10.1016/j.jnutbio.2014.10.016
[15]	YANG J L, ZHU A, XIAO S, et al. Anthraquinones in the aqueous extract of cassiae semen cause liver injury in rats through lipid metabolism disorder[J]. Phytomedicine,2019,64:153059. doi: 10.1016/j.phymed.2019.153059
[16]	GUO Q H, ZHANG M, ZHOU G Y, et al. Highly sensitive simultaneous electrochemical detection of hydroquinone and catechol with three-dimensional N-doping carbon nanotube film electrode[J]. Journal of Electroanalytical Chemistry,2016,760:15−23. doi: 10.1016/j.jelechem.2015.11.034
[17]	SUN H N, MU T H, LIU X L, et al. Purple sweet potato (Ipomoea batatas L. ) anthocyanins: Preventive effect on acute and subacute alcoholic liver damage and dealcoholic effect[J]. Journal of Agricultural and Food Chemistry,2014,62(11):2364−2373. doi: 10.1021/jf405032f
[18]	LEE H I, MCGREGOR R A, CHOI M S, et al. Low doses of curcumin protect alcohol-induced liver damage by modulation of the alcohol metabolic pathway, CYP2E1 and AMPK[J]. Life Sciences,2013,93(18-19):693−699. doi: 10.1016/j.lfs.2013.09.014
[19]	BAJAJ J S. Alcohol, liver disease and the gut microbiota[J]. Nature Reviews Gastroenterology & Hepatology,2019,16(4):235−246.
[20]	YANG L L, WU D F, WANG X D, et al. Cytochrome P4502E1, oxidative stress, JNK and autophagy in acute alcohol-induced fatty liver[J]. Free Radical Biology and Medicine,2012,53(5):1170−1180. doi: 10.1016/j.freeradbiomed.2012.06.029
[21]	LEUNG T M, NIETO N. CYP2E1 and oxidant stress in alcoholic and non-alcoholic fatty liver disease[J]. Journal of Hepatology,2013,58(2):395−398. doi: 10.1016/j.jhep.2012.08.018
[22]	DAI C, LI D, GONG L, et al. Curcumin ameliorates furazolidone-induced DNA damage and apoptosis in human hepatocyte L02 Cells by inhibiting ROS production and mitochondrial pathway[J]. Molecules,2016,21(8):1061. doi: 10.3390/molecules21081061
[23]	SUN X, WANG P, YAO L P, et al. Paeonol alleviated acute alcohol-induced liver injury via SIRT1/Nrf2/NF-B signaling pathway[J]. 2018, 60: 110−117.
[24]	HE M, PAN H, CHANG R C, et al. Activation of the Nrf2/HO-1 antioxidant pathway contributes to the protective effects of Lycium barbarum polysaccharides in the rodent retina after ischemia-reperfusion-induced damage[J]. PloS One,2014,9(1):e84800. doi: 10.1371/journal.pone.0084800
[25]	NGUYEN T, NIOI P, PICKETT C B. The Nrf2-antioxidant response element signaling pathway and its activation by oxidative stress[J]. Journal of Biological Chemistry,2009,284(20):13291−13295. doi: 10.1074/jbc.R900010200
[26]	CAO M, WANG H, GUO L, et al. Dibenzoylmethane protects against CCl₄-induced acute liver injury by activating Nrf2 via JNK, AMPK, and calcium signaling[J]. AAPS Journal,2017,19(6):1703−1714. doi: 10.1208/s12248-017-0133-1
[27]	沈艳艳, 任婷婷. 蓝莓及蓝莓联合益生菌对酒精性脂肪性肝病的保护作用[J]. 贵阳医学院学报,2018,43(11):1241−1246,1251. [SHEN Y Y, REN T T. Effects of dietary blueberry and blueberry combined with probiotics on alcoholic fatty liver disease[J]. Journal of Guiyang Medical College,2018,43(11):1241−1246,1251.
[28]	CHENG M, ZHU J, REN T, et al. The combination of blueberry juice and probiotics reduces apoptosis of alcoholic fatty liver of mice by affecting SIRT1 pathway[J]. Drug Design Development & Therapy,2016,10:1649−1661.
[29]	DAI W B, CHEN C, FENG H T, et al. Protection of ficus pandurata hance against acute alcohol-induced liver damage in mice via suppressing oxidative stress, inflammation, and apoptosis[J]. Journal of Ethnopharmacology,2021,275:114140. doi: 10.1016/j.jep.2021.114140
[30]	HONG S, KIM Y, SUNG J, et al. Jujube (Ziziphus jujuba Mill.) protects hepatocytes against alcohol-induced damage through Nrf2 activation[J]. Evid Based Complement Alternat Med,2020,2020(2):1−8.

Supplements (0)

Cited By

Cited by

Periodical cited type(4)

1.	柳富杰，黄释慧，潘莉莉. 纳米碳酸钙表面改性沸石对模拟蔗汁中酚酸的吸附性能. 食品与机械. 2024(01): 33-39 .
2.	玉澜，时倩倩，文胜，陈燕萌，兰兴先，蒋才云. 白云石/三聚氰胺复合材料制备及吸附结晶紫性能研究. 化学研究与应用. 2024(08): 1718-1724 .
3.	陈燕萌，陈广成，蒋才云. 沃柑皮基活性炭的制备及其对结晶紫的吸附研究. 化学研究与应用. 2023(06): 1387-1395 .
4.	王未君，杨博，李文林，马旋，刘昌盛. 不同种类二氧化硅的表征及其在浓香菜籽油低温吸附精炼中的应用. 食品科学. 2023(16): 1-7 .