Simultaneous Determination of Six Sugars in Beef Hydrolysates by Ion Chromatography with Pulsed Amperometric Detection

CHEN Xiuhong; JI Peng; HE Guoliang; XIA Ran; LI Zuming; LIU Jia

doi:10.13386/j.issn1002-0306.2021070122

Volume 43 Issue 11

May 2022

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Science and Technology of Food Industry > 2022 > 43(11): 267-275. > DOI: 10.13386/j.issn1002-0306.2021070122

CHEN Xiuhong, JI Peng, HE Guoliang, et al. Simultaneous Determination of Six Sugars in Beef Hydrolysates by Ion Chromatography with Pulsed Amperometric Detection[J]. Science and Technology of Food Industry, 2022, 43(11): 267−275. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021070122.

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Simultaneous Determination of Six Sugars in Beef Hydrolysates by Ion Chromatography with Pulsed Amperometric Detection

CHEN Xiuhong^{1, 2,},
JI Peng³,
HE Guoliang¹,
XIA Ran¹,
LI Zuming³,
LIU Jia^{1, 2, ,}

1.
Internal Trade Food Science Research Institute Co., Ltd., National Non-staple Food Quality Supervision and Inspection Center, Beijing 102209, China
2.
COFCO Nutrition and Health Research Institute Co., Ltd., Beijing Key Laboratory of Nutrition Health and Food Safety, Beijing 102209, China
3.
College of Biochemical Engineering, Beijing Union University, Beijing 100023, China

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Received Date: July 08, 2021
Available Online: April 06, 2022

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Abstract

Abstract

The aim of this study is to establish an ion chromatography method for simultaneous determination of glucose, fructose, arabinose, galactose, mannose and ribose in beef hydrolysates. The pretreatment and chromatography conditions were optimized. Finally, the protein was precipitated by 1% trichloroacetic acid and polysaccharide was hydrolyzed by 4 mol/L sulfuric acid. The CarboPac^TM PA20 column was selected for separation. 5 mmol/L sodium hydroxide solution was used as the eluent for gradient elution, with the detection of pulse amperometric detector(PAD). The results showed that the six sugars could be separated completely under the above conditions, the linear relationship of glucose and other sugars within the range of 0.25~2.50 μg/mL and 0.025~0.25 μg/mL, respectively, was good. And the correlation coefficient was more than 0.998. The RSD of method was within 2.3%~5.5%, and the recovery was within 79.6%~116.5%. The method has the advantages of simple operation, high sensitivity and good resolution, and can be used to determine the content of sugars in beef hydrolysates accurately and rapidly.
- ion chromatography,
- pulsed amperometric detection,
- beef,
- hydrolysates,
- monosaccharides

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[1]	麻海峰, 常征, 杨光辉. 牛肉的营养价值及排酸、速冻工艺研究[J]. 农业科技与装备,2010(7):34−36. [MA Haifeng, CHANG Zheng, YANG Guanghui. Value of beef and its acid discharge and quick-freeze techniques[J]. Agricultural Science & Technology and Equipment,2010(7):34−36. doi: 10.3969/j.issn.1674-1161.2010.07.011
[2]	董建国. 超高压及谷氨酰胺转胺酶对牛肉肠品质的影响[D]. 洛阳: 河南科技学院, 2013. DONG Jianguo. Effects of high hydrostatic pressure and microbial transglutaminase treatment on the qualities of beef sausage[D]. Luoyang: Henan Institute of Science and Technology, 2013.
[3]	金萍, 丁洪流, 李培, 等. 2013年苏州地区肉及其制品掺假情况调查[J]. 中国食品卫生杂志,2014,26(2):168−172. [JIN Ping, DING Hongliu, LI Pei, et al. Analysis of meat products adulterated in Suzhou area in 2013[J]. Chinese Journal of Food Hygiene,2014,26(2):168−172.
[4]	程灵豪, 焦永亮. 白肌肉、注水肉、注胶肉的鉴别检验方法[J]. 中国动物检疫,2015,32(4):28−31. [CHENG Linghao, JIAO Yongliang. Inspection methods of the PSE meat, water-injected meat and gel-injected meat[J]. China Animal Health Inspection,2015,32(4):28−31. doi: 10.3969/j.issn.1005-944X.2015.04.009
[5]	赵改名, 孟子晴, 祝超智, 等. 不同食用胶与牛肉糜结合力及凝胶性能的比较[J]. 现代食品科技,2020,36(4):7. [ZHAO Gaiming, MENG Ziqing, ZHU Chaozhi, et al. Comparison of the binding and gel properties between different edible gums and beef paste[J]. Modern Food Science and Technology,2020,36(4):7.
[6]	徐宏蕾, 王亚珍, 谢晶, 等. 壳寡糖对壳聚糖—明胶复合膜的牛肉保鲜性能影响[J]. 食品与生物技术学报,2018,37(10):46−51. [XU Honglei, WANG Yazhen, XIE Jing, et al. Effect of chitosan oligosaccharide on preservation of beef of gelatin-chitosan composite films[J]. Journal of Food Science and Biotechnology,2018,37(10):46−51.
[7]	白明, 尹金兰. 肉制品中常见食用胶及其应用[J]. 食品安全导刊,2010(1):50−52. [BAI Ming, YIN Jinlan. Common edible glue in meat products and its application[J]. China Food Safety Magazine,2010(1):50−52.
[8]	张奇, 冯峰, 杨淑玲, 等. 不同产地霸王花粗多糖的单糖组成及体外抗氧化活性[J]. 上海交通大学学报(农业科学版),2019,37(2):83−89. [ZHANG Qi, FENG Feng, YANG Shuling, et al. Studies on monosaccharide composition and antioxidant activity of crude polysaccharide from Hylocereus undatus (Haw.) Urilt. et. Rose of different regions[J]. Journal of Shanghai Jiaotong University (Agricultural Science),2019,37(2):83−89.
[9]	张文平. 植物乳杆菌LPC-1胞外多糖发酵, 结构解析及诱导水稻抗逆性研究[D]. 南昌, 江西农业大学, 2019. ZHANG Wenping. Fermentation, structural characterization and induction of stress resistance in rice of exopolysaccharides from Lactobacillus plantarum LPC-1[D]. Nanchang: Jiangxi Agricultural University, 2019.
[10]	饶君凤, 吕伟德, 章卓梁, 等. 离子色谱-脉冲安培法分析西红花多糖的单糖组成[J]. 浙江大学学报(理学版),2015,42(3):330−333, 346. [RAO Junfeng, LYU Weide, ZHANG Zhuoliang, et al. Monosaccharide composition analysis of polysaccharides in saf flower by ion chromatography pulsed amperometric detection[J]. Journal of Zhejiang University (Science Edition),2015,42(3):330−333, 346.
[11]	柴银, 王虹, 刘雅萌, 等. 离子色谱测定多糖的单糖组成的方法学研究及其应用[J]. 药物生物技术,2016,23(4):332−336. [CHAI Yin, WANG Hong, LIU Yameng, et al. Methodology study of monosaccharide composition by HPAEC-PAD and its application[J]. Pharmaceutical Biotechnology,2016,23(4):332−336.
[12]	李国强, 尹平河, 赵玲, 等. 离子色谱-积分脉冲安培检测法测定大蒜多糖的单糖组成[J]. 分析测试学报,2007(3):401−403, 408. [LI Guoqiang, YIN Pinghe, ZHAO Ling, et al. Determination of monosaccharides in garlic polysaccharide by ion chromatography-integrated pulsed amperometric detection[J]. Journal of Instrumental Analysis,2007(3):401−403, 408. doi: 10.3969/j.issn.1004-4957.2007.03.026
[13]	徐佳佳, 刘玉峰, 孔凡华, 等. 离子色谱-脉冲安培法检测食品中功能性低聚异麦芽糖[J]. 食品工业科技,2021,42(13):262−267. [XU Jiajia, LIU Yufeng, KONG Fanhua, et al. Determination of isomalto oligosaccharide in foods by ion chromatography-pulsed amperometric detection[J]. Science and Technology of Food Industry,2021,42(13):262−267.
[14]	JASZCZAK E, KOZIOL K, KIELBRATOWSKA B, et al. Application of ion chromatography with pulsed amperometric detection for the determination of trace cyanide in biological samples, including breast milk[J]. Journal of Chromatography B,2019,1110-1111:36−42. doi: 10.1016/j.jchromb.2019.02.008
[15]	彭丽诗, 陈琼, 黄盼, 等. 离子色谱-脉冲安培法同时测定食品中9种糖和糖醇的含量[J]. 食品工业科技,2020,41(9):250−254, 259. [PENG Lishi, CHEN Qiong, HUANG Pan, et al. Simultaneous determination of 9 sugars and sugar alcohols in foods by Ion chromatography with pulsed amperometric detection[J]. Science and Technology of Food Industry,2020,41(9):250−254, 259.
[16]	陈越, 宋振康, 张海悦. 三氯乙酸法脱除龙葵果多糖中蛋白质的工艺优化[J]. 食品与发酵工业,2020,46(24):198−203. [CHEN Yue, SONG Zhenkang, ZHANG Haiyue, et al. Study on optimization of response surface for the removement of proteinfrom polysaccharide of Solanum nigrum fruit by chloroacetic acid method[J]. Food and Fermentation Industries,2020,46(24):198−203.
[17]	刘瑀, 方志强, 刘宇馨, 等. 虾夷扇贝多糖提取及纯化方法的优化[J]. 食品科学,2017,38(18):208−213. [LIU Yu, FANG Zhiqiang, LIU Yuxin, et al. Optimization of extraction and purification of polysaccharides from Patinopecten yessoensis[J]. Food Science,2017,38(18):208−213. doi: 10.7506/spkx1002-6630-201718033
[18]	市场监管总局. 市场监管总局关于发布《畜肉中卡拉胶的测定》食品补充检验方法的公告 [2018年第10号][J]. 中国食品卫生杂志,2018,30(4):1. [General Administration of Market Supervision. Announcement of General Administration of Market Supervision on Issuing supplementary food inspection methods for determination of carrageenan in animal meat [2018 No.10][J]. Chinese Journal of Food Hygiene,2018,30(4):1.
[19]	孔俊豪, 史劲松, 孙达峰, 等. 瓜尔胶及其衍生物最新研究进展[J]. 食品研究与开发,2009,30(4):167−170. [KONG Junhao, SHI Jinsong, SUN Dafeng, et al. Recent research progress in guar gum and its derivative[J]. Food Research and Development,2009,30(4):167−170. doi: 10.3969/j.issn.1005-6521.2009.04.050
[20]	ZHU Zuoyi, ZHANG Yu, WANG Junhong, et al. Characterization of sugar composition in Chinese royal jelly by ion chromatography with pulsed amperometric detection[J]. Journal of Food Composition and Analysis,2019,78(5):101−107.
[21]	VERONIQUE S, SEAN A, KOMMER B, et al. Determination of fructans in infant formula and adult/pediatric nutritional formula by anion-exchange chromatography with pulsed amperometric detection after enzymatic treatment: Collaborative study, final action 2016.14[J]. Journal of AOAC International,2020(5):1301−1317.
[22]	中华人民共和国国家卫生和计划生育委员会, 国家食品药品监督管理总局. GB 5009.89-2016 食品安全国家标准食品中烟酸和烟酰胺的测定[S]. 北京: 中国标准出版社, 2016. National Health and Family Planning Commission of the People’s Republic of China, China Food and Drug Administration. GB 5009.89-2016 National Standard of the People’s Republic of China Determination of niacin and nicotinamide in food[S]. Beijing: China Standards Press, 2016.
[23]	HAGERMAN A E, BUTLER L G. Protein precipitation method for the quantitative determination of tannins[J]. Journal of Agricultural Food Chemistry,1978,26(4):568−569.
[24]	李秀琴, 杨总, 张庆合. 测定肉制品中6种食品添加剂的样品前处理方法[J]. 理化检验(化学分册),2013,49(2):186−189. [LI Xiuqin, YANG Zong, ZHANG Qinghe. Pretreatment of samples of meat products for simultaneous determination of 6 food additives[J]. Physical Testing and Chemical Analysis (Part B: Chemical Analysis),2013,49(2):186−189.
[25]	阎智锋, 连洁, 赵舟, 等. 亚临界水中葡萄糖转化生成5-羟甲基糠醛的理论研究[J]. 燃料化学学报,2021,49(8):1122−1131. [YAN Zhifeng, LIAN Jie, ZHAO Zhou, et al. Theoretical insight into the conversion of glucose to 5-hydroxymethylfurfural in subcritical water[J]. Journal of Fuel Chemistry and Technology,2021,49(8):1122−1131. doi: 10.1016/S1872-5813(21)60124-X
[26]	孙欣, 张承明, 时川, 等. 红枣黑变前后感官特性及功能和香气成分的比较[J]. 中国食物与营养,2019,25(1):4. [SUN Xin, ZHANG Chengming, SHI Chuan, et al. Comparison of sensory characteristics, function and aroma components between red jujube and black jujube[J]. Food and Nutrition in China,2019,25(1):4.
[27]	路鹏, 江滔, 李国学. 木质纤维素乙醇发酵研究中的关键点及解决方案[J]. 农业工程学报,2006,22(9):237−240. [LU Peng, JIANG Tao, LI Guoxue. Key points of ethanol fermentation of lignocellulose and resolving methods[J]. Transactions of the CSAE,2006,22(9):237−240. doi: 10.3321/j.issn:1002-6819.2006.09.050
[28]	MARTIN C, JONSSON L J. Comparison of the resistance of industrial and laboratory strains of Saccharomyces and Zygosaccharomyces to lignocellulose-derived fermentation inhibitors[J]. Enzyme and Microbial Technology,2003,32(3):855−858.
[29]	宋卫得, 苏征, 惠希东, 等. 离子色谱-积分脉冲安培检测法同时测定酱油中20种氨基酸和5种糖[J]. 色谱,2019,37(9):996−1003. [SONG Weide, SU Zheng, HUI Xidong, et al. Simultaneous determination of twenty amino acids and six carbohydrates in soy sauce by ion chromatography with integrated pulsed amperometric detection[J]. Chinese Journal of Chromatography,2019,37(9):996−1003. doi: 10.3724/SP.J.1123.2019.03030
[30]	朱伟, 宁啸骏, 杜茹芸. 高效离子色谱法检测婴幼儿配方奶粉中的功能性低聚糖[J]. 食品科学,2017,38(24):224−228. [ZHU Wei, NING Xiaojun, DU Ruyun. Determination of functional oligosaccharides in infant formula milk powder by high performance ion chromatography[J]. Food Science,2017,38(24):224−228. doi: 10.7506/spkx1002-6630-201724036
[31]	黎小兰, 陈彩云, 蔡伟江, 等. 液相色谱-质谱法测定保健食品乳清蛋白粉类中叶酸含量的前处理条件的优化[J]. 食品安全质量检测学报,2017,8(8):3085−3091. [LI Xiaolan, CHEN Caiyun, CAI Weijiang, et al. Optimization of pretreatment methods for determination of folic acid content in health food of whey protein powder by liquid chromatography-mass chromatography[J]. Journal of Food Safety and Quality,2017,8(8):3085−3091. doi: 10.3969/j.issn.2095-0381.2017.08.041
[32]	KOUTSIDIS G, ELMORE J S, ORUNA-CONCHA M J, et al. Water-soluble precursors of beef flavour: I. Effect of diet and breed[J]. Meat Science,2008,79(1):124−130. doi: 10.1016/j.meatsci.2007.08.008
[33]	MACY R L, NAUMANN H D, BAILEYM E. Water-soluble flavor and odor precursors of meat. (I) Qualitative study of certain amino acids, carbohydrates, non amino-acid nitrogen compounds and phosphoric acid esters of beef, pork, and lamb[J]. Journal of Food Science,1964,29:136−141. doi: 10.1111/j.1365-2621.1964.tb01708.x
[34]	MACY R L, NAUMANN H D, BAILEY M E. Water-soluble flavor and odor precursors of meat. (II) Effects of heating on amino nitrogen constituents and carbohydrates in lyophilized diffusates from aqueous extracts of beef, pork and lamb[J]. Journal of Food Science,1964,29:142−148. doi: 10.1111/j.1365-2621.1964.tb01709.x
[35]	MACY R L, NAUMANN H D, BAILEY M E. Water-soluble flavor and odor precursors of meat. 5. Influence of heating on acidextractable non-nucleotide chemical constituents of beef, lamb and pork[J]. Journal of Food Science,1970,35:83−87. doi: 10.1111/j.1365-2621.1970.tb12375.x
[36]	范田慧, 孙延平, 乔威杰, 等. 半乳甘露聚糖的提取纯化, 结构特征以及应用研究进展[J]. 化学工程师,2021,35(5):5. [FAN Tianhui, SUN Yanping, QIAO Weijie, et al. Progress in extraction, purification, structural characteristics and application of galactomannan[J]. Chemical Engineer,2021,35(5):5.

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