Optimization of Microwave-assisted Extraction of Total Glycosides from <i>Moringa oleifera</i> Seeds and Its Antioxidant Activity

TANG Xingnan; LUAN Kaiwen; WANG Rongxiang; REN Shuai; ZHANG Wanzhong; ZHU Jianxing

doi:10.13386/j.issn1002-0306.2021070078

Volume 43 Issue 4

Feb. 2022

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Science and Technology of Food Industry > 2022 > 43(4): 246-253. > DOI: 10.13386/j.issn1002-0306.2021070078

TANG Xingnan, LUAN Kaiwen, WANG Rongxiang, et al. Optimization of Microwave-assisted Extraction of Total Glycosides from Moringa oleifera Seeds and Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2022, 43(4): 246−253. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021070078.

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Optimization of Microwave-assisted Extraction of Total Glycosides from Moringa oleifera Seeds and Its Antioxidant Activity

College of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology, Shenyang 110142, China

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Received Date: July 06, 2021
Available Online: December 12, 2021

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Abstract

Abstract

In order to effectively utilize and develop Moringa oleifera seeds, the glycosides in Moringa oleifera seeds were extracted by microwave-assisted method with ethanol as solvent. Based on the single factor experiment, the extraction process was optimized by response surface methodology. The antioxidant activities in vitro of each polar part of the optimized Moringa oleifera seeds extract were determined by salicylic acid method, ABTS method and FRAP method. The results showed that the optimal extraction conditions were as follows: Ethanol volume fraction 81%, solid-liquid ratio 1:30 g/mL, extraction time 20 min, microwave power 600 W. The order of antioxidant activity in vitro was ethyl acetate>petroleum ether > n-butanol > water. The results indicated that the microwave-assisted extraction of glycosides from Moringa oleifera seeds and the optimization of the extraction process of total glycosides from Moringa oleifera seeds by response surface methodology was feasible. In vitro, antioxidant activity tests showed that the polar parts of ethanol extracts of Moringa oleifera seeds had free radical scavenging ability, which laid a foundation for the further development of natural antioxidants.

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