Rapid Determination of 21 Mycotoxins in Milk and Dairy Products by Ultra Performance Liquid Chromatography-quadrupole/orbitrap High Resolution Mass Spectrometry

HE Zhuolin; MU Lei; WANG Tao; WANG Liang; XIE Yunfeng

doi:10.13386/j.issn1002-0306.2021070077

Volume 43 Issue 6

Mar. 2022

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Science and Technology of Food Industry > 2022 > 43(6): 302-310. > DOI: 10.13386/j.issn1002-0306.2021070077

HE Zhuolin, MU Lei, WANG Tao, et al. Rapid Determination of 21 Mycotoxins in Milk and Dairy Products by Ultra Performance Liquid Chromatography-quadrupole/orbitrap High Resolution Mass Spectrometry[J]. Science and Technology of Food Industry, 2022, 43(6): 302−310. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021070077.

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Rapid Determination of 21 Mycotoxins in Milk and Dairy Products by Ultra Performance Liquid Chromatography-quadrupole/orbitrap High Resolution Mass Spectrometry

1.
College of Life Science and Technology, Xinjiang University, Urmuqi 830046, China
2.
Beijing Key Laboratory of Nutrition Health and Food Safety, COFCO Nutrition & Health Research Institute, Beijing 102209, China

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Received Date: July 06, 2021
Available Online: January 16, 2022

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Abstract

Abstract

A method for the simultaneous determination of 21 mycotoxins in milk and dairy products using ultra performance liquid chromatography-quadrupole/orbitrap high-resolution mass spectrometry technology was established. After the milk and dairy products were extracted with acidified acetonitrile, they were purified by a Captiva-EMR Lipid purification column and chromatographically separated by a C₁₈ column. The mass spectrometry adopt full scan data-dependent MS/MS acquisition mode (Full MS/ddMS²) detected in positive and negative ion modes, and quantified by external standard method. The 21 mycotoxins all had a good linear relationship within a certain concentration range, the correlation coefficients (r) were greater than 0.99, the limits of quantification were 1.0~93.8 μg/kg, and the average recoveries of all mycotoxins were 60.3%~107.0% at three different levels, and the relative standard deviations (RSD) ranged between 0.4%~14.4%. This method was used to detect commercially available powdered and liquid milk and dairy products. Aflatoxin B₂ was detected in a fruit-added yogurt sample. The method was fast, accurate, suitable for the rapid determination of multiple mycotoxins in milk and dairy products, which could be used for efficient screening of mycotoxins risks.

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