Effects of Storage Temperature on Sugar Metabolism and Related Enzyme Activities of Postharvest Longan Fruits

GUAN Boyang; YIN Feilong; LIU Yunfen; LIAO Lingyan; CHEN Zhenlin; SHUAI Liang

doi:10.13386/j.issn1002-0306.2021060261

Volume 43 Issue 5

Feb. 2022

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Science and Technology of Food Industry > 2022 > 43(5): 348-355. > DOI: 10.13386/j.issn1002-0306.2021060261

GUAN Boyang, YIN Feilong, LIU Yunfen, et al. Effects of Storage Temperature on Sugar Metabolism and Related Enzyme Activities of Postharvest Longan Fruits[J]. Science and Technology of Food Industry, 2022, 43(5): 348−355. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021060261.

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Effects of Storage Temperature on Sugar Metabolism and Related Enzyme Activities of Postharvest Longan Fruits

1.
School of Food Science, Dalian Polytechnic University, Dalian 116034, China
2.
School of Food and Biological Engineering/Institute of Food Science and Engineering Technology, Hezhou University, Hezhou 542899, China

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Received Date: June 28, 2021
Available Online: December 29, 2021

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Abstract

Abstract

To study the effects of different storage temperatures (25, 15, 4 ℃) after harvest on the sugar components and related enzyme activities of "Shixia" longan fruits. The longan pulp soluble solid content (SSC), the content of three main sugars and the changes in the activities of sugar metabolism-related enzymes were measured. Principal component analysis and load factor analysis were performed on samples at different storage temperatures and various periods, and the correlation analysis of each index was performed. The results showed that the SSC of longan stored at 25 ℃ decreased the fastest, from 23.5% to 22.0% after 8 days of storage, and the sucrose content decreased the fastest, but the glucose and fructose content showed an upward trend; both 15℃ and 4 ℃ could effectively delay SSC. The decrease in SSC of longan stored at 4 ℃ was slower and the sucrose and glucose content decreased the slowest, but the decrease in fructose content was faster than 15 ℃. The acid invertase (AI), neutral invertase (NI) and sucrose synthase (SS) activities of longan pulp stored at different temperatures all increase with the storage time, the amplitude and speed of the increase was the fastest at 25 °C, at 15 °C was secondly, and the 4 ℃ rise was the most gentle, indicating that low temperature significantly inhibited the increase in the activity of sucrose metabolizing enzymes. Correlation analysis showed that the sucrose content of longan stored at different temperatures had a very significant positive correlation with SSC, indicating that SSC could reflect the sucrose content to a certain extent; the sucrose content had a significant positive correlation with the glucose content, and it also had significant negative correlation with AI, NI, and SS. It was inferred that glucose and fructose were degraded from sucrose; glucose content and fructose content were extremely significantly positively correlated, but the two decline patterns in 15 ℃ and 4 ℃ storage fruits were inconsistent, suggesting that the consumption of the two was different. The above results indicate that low temperature could significantly inhibit the increase of sugar metabolism and related enzyme activities in longan fruit, thereby slowing down the decomposition of sugar during storage and prolonging the storage time. Among them, the storage effect at 4 ℃ was the best.
- longan,
- sugar metabolism,
- enzyme activity,
- storage temperature,
- correlation analysis

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References

[1]	张容鹄, 冯建成, 邓浩, 等. “储良”和“石硖”龙眼果实采后品质变化和贮藏性比较[J]. 保鲜与加工,2017,17(5):7−13. [ZHANG R H, FENG J C, DENG H, et al. Comparison of postharvest quality changes and storability between ‘Shixia’ and ‘Chuliang’ longan fruits[J]. Storage and Process,2017,17(5):7−13. doi: 10.3969/j.issn.1009-6221.2017.05.002
[2]	郑少泉, 曾黎辉, 张积森, 等. 新中国果树科学研究70年——龙眼[J]. 果树学报,2019,36(10):1414−1420. [ZHENG S Q, ZENG L H, ZHANG J S, et al. Fruit scientific research in new china in the past 70 years: Longan[J]. Joumal of Fruit Science,2019,36(10):1414−1420.
[3]	LUO T, SHUAI L, LAI T, et al. Up-regulated glycolysis, TCA, fermentation and energy metabolism promoted the sugar receding in 'Shixia' longan (Dimocarpus longan Lour.) pulp[J]. Scientia Horticulturae,2021,281:109998. doi: 10.1016/j.scienta.2021.109998
[4]	TAO L, LIANG S, LIAO L, et al. Soluble acid invertases act as key factors influencing the sucrose/hexose ratio and sugar receding in longan pulp[J]. Journal of Agricultural and Food Chemistry,2018,67(1):352−363.
[5]	林思思, 蒲小秋, 费光雪, 等. 冬枣和骏枣果实发育过程中糖组分及代谢酶活性变化的研究[J]. 黑龙江农业科学,2018(7):8−11. [LIN S S, PU X Q, FEI G X, et al. Study on changes of sugar components and metabolic enzyme activities during fruit development of dongzao and junzao[J]. Heilongjiang Agricultural Sciences,2018(7):8−11.
[6]	周慧娟, 叶正文, 苏明申, 等. 4个品种黄桃果实硬核期后和采后糖酸代谢规律[J]. 上海农业学报,2017,33(1):50−55. [ZHOU H J, YE Z W, SU M S, et al. Sugar and acid metabolism regularity with four varieties of yellow-peach after flull blooming and during shelf[J]. Acta Agricultural Shanghai,2017,33(1):50−55.
[7]	潘俨, 孟新涛, 车凤斌, 等. 库尔勒香梨果实发育成熟的糖代谢和呼吸代谢响应特征[J]. 中国农业科学,2016,49(17):3391−3412. [PAN Y, MENG X T, CHE F B, et al. Metabolic profiles of sugar metabolism andrespiratory metabolism of korla pear (Pyrus sinkiangensis Yu) throughout fruit development and ripening[J]. Scientia Agricultura Sinica,2016,49(17):3391−3412. doi: 10.3864/j.issn.0578-1752.2016.17.013
[8]	YATIV M, HARARY I, WOLF S. Sucrose accumulation in watermelon fruits: Genetic variation and biochemical analysis[J]. Journal of Plant Physiology,2009,167(8):589−596.
[9]	赵云峰, 林河通, 林娇芬, 等. 龙眼果实采后呼吸强度、细胞膜透性和品质的变化[J]. 福建农业大学学报,2005(2):263−268. [ZHANG Y F, LIN H T, LIN J F, et al. Changes of respiration rate, cell membranepermeability and quality in post-harvest longan fruit[J]. Journal of Fujian Agriculture and Forestry University,2005(2):263−268.
[10]	马凤凰. 龙眼果实发育过程中糖代谢的研究[D]. 福州: 福建农林大学, 2009. MA F H. Study on the mechanisms of sucrose-metabolizing during the development of longan fruit[D]. Fuzhou: Fujian Agriculture and Forestry University, 2009.
[11]	陈锦, 林毅雄, 林育钊, 等. 低温贮藏对‘松风本’龙眼果实品质和耐贮性的影响[J]. 热带作物学报,2020,41(11):2314−2321. [CHEN J, LIN Y X, LIN Y Z, et al. Effects of low temperature storage on quality and storability of ‘Songfengben’ longan fruit[J]. Chinese Journal of Tropical Crops,2020,41(11):2314−2321. doi: 10.3969/j.issn.1000-2561.2020.11.024
[12]	帅良, 谷李桃, 刘文浩, 等. 不同龙眼品种果实退糖特性分析[J]. 热带作物学报,2016,37(10):1900−1907. [SHUAI L, GU L T, LIU W H, et al. Characteristic analysis of sugar returning in the fruit of different longan cultivars[J]. Chinese Journal of Tropical Crops,2016,37(10):1900−1907. doi: 10.3969/j.issn.1000-2561.2016.10.009
[13]	赵云峰, 林河通, 王静, 等. 热处理对龙眼果实采后生理和贮藏品质的影响[J]. 中国食品学报,2014,14(5):124−133. [ZHAO Y F, LIN H T, WANG J, et al. Effects of heat treatment on postharvest physiology and storage quality of longan fruits[J]. Journal of Chinese Institute of Food Science and Technology,2014,14(5):124−133.
[14]	帅良, 薛晓清, 牛佳佳, 等. 龙眼果实发育过程中果糖激酶活性及其基因表达分析[J]. 华南农业大学学报,2015,36(5):99−104. [SHUAI L, XUE X Q, NIU J J, et al. Analyses of the fructokinase activity and its gene expression during the development of longan fruits[J]. Journal of South China Agricultural University,2015,36(5):99−104. doi: 10.7671/j.issn.1001-411X.2015.05.017
[15]	AKIRA K, YUKO T, TAKAYA M, et al. Differential expression of three sucrose-phosphate synthase isoforms during sucrose accumulation in citrus fruits (Citrus unshiu Marc.)[J]. Plant Science,1999,140(2):169−178. doi: 10.1016/S0168-9452(98)00217-9
[16]	HUBBARD N L, PHARR D M, HUBER S C. Sucrose phosphate synthase and other sucrose metabolizing enzymes in fruits of various species[J]. Physiologia Plantarum,1991,82(2):191−196. doi: 10.1111/j.1399-3054.1991.tb00080.x
[17]	LOWELL C A, TOMLINSON P T, KOCH K E. Sucrose-metabolizing enzymes in transport tissues and adjacent sink structures in developing citrus fruit[J]. Plant Physiology,1989,90(4):1394−1402. doi: 10.1104/pp.90.4.1394
[18]	廖光联, 钟敏, 黄春辉, 等. 果实糖代谢及其相关酶基因研究进展[J]. 江西农业大学学报,2020,42(1):187−195. [LIAO G L, ZHONG M, HUANG C H, et al. Progress in research on sugar metabolism and related enzyme genes in fruit[J]. Acta Agriculturae Universitatis Jiangxiensis,2020,42(1):187−195.
[19]	LI D, CHENG Y, DONG Y, et al. Effects of low temperature conditioning on fruit quality and peel browning spot in ‘Huangguan’ pears during cold storage[J]. Postharvest Biology and Technology,2017,131:68−73. doi: 10.1016/j.postharvbio.2017.05.005
[20]	梁琪琪, 张润光, 刘皓涵, 等. 低温结合薄膜包装对石榴果实采后生理及贮藏品质的影响[J]. 食品与发酵工业,2020,46(8):187−191. [LIANG Q Q, ZHANG R G, LIU H H, et al. Effects of low temperature combined with film packaging on postharvest physiology and storage quality of pomegranate fruit[J]. Food and Fermentation Industries,2020,46(8):187−191.
[21]	李丽, 李杰民, 孙健, 等. 仲丁醇对采后龙眼低温贮藏特性的影响[J]. 南方农业学报,2017,48(12):2247−2252. [LI L, LI J M, SUN J, et al. Effects of 2-butanol on characteristics of postharvest longan fruits stored under low temperature[J]. Journal of Southern Agriculture,2017,48(12):2247−2252. doi: 10.3969/j.issn.2095-1191.2017.12.21
[22]	周慧娟, 王忠, 叶正文, 等. 不同类型桃果实采后糖酸代谢的差异性分析[J]. 经济林研究,2016,34(3):30−41. [ZHOU H J, WANG Z, YE Z W, et al. Difference analysis on postharvest sugar and acid metabolism in different types of peach fruits[J]. Nonwood Forest Research,2016,34(3):30−41.
[23]	李昌宝, 孙健, 李丽, 等. 贮藏温度对香蕉糖酸组分的影响[J]. 食品工业科技,2017,38(7):305−310. [LI C B, SUN J, LI L, et al. Effect of storing temperatures on the sugar and acid compositions in banana fruits[J]. Science and Teghnology of Food Industry,2017,38(7):305−310.
[24]	王贵元, 夏仁学, 曾祥国, 等. 不同温度贮藏红肉脐橙果肉主要色素和糖含量的变化及其相关性[J]. 西北农业学报,2007(3):180−183. [WANG G Y, XIA R X, ZENG X G, et al. Changes of main pigments and sugar concentration in flesh of cara cara navel orange and their correlation in different temperature storage[J]. Acta Agriculturae Boreali-occidentalis Sinica,2007(3):180−183. doi: 10.3969/j.issn.1004-1389.2007.03.044
[25]	SWEETMAN C, DELUC L G, CRAMER G R, et al. Regulation of malate metabolism in grape berry and other developing fruits[J]. Phytochemistry,2009,70(11/12):1329−1344.
[26]	帅良. 龙眼果实发育和贮藏期间糖代谢及其相关基因研究[D]. 广州: 华南农业大学, 2012. SHUAI L. Study on sugar metabolism and related genes during longan fruit development and storage[D]. Guangzhou: South China Agricultural University, 2012.
[27]	张强, 代文婷, 李冀新, 等. 糖代谢对甜瓜果实后熟软化的影响[J]. 食品与发酵工业,2020,46(1):112−117. [ZHANG Q, DAI W T, LI J X, et al. Effects of sugar metabolism on post-ripening and softening of melon fruits[J]. Food and Fermentation Industries,2020,46(1):112−117.
[28]	BARRATT D, DERBYSHIRE P, FINDLAY K, et al. Normal growth of Arabidopsis requires cytosolic invertase but not sucrose synthase[J]. Proceedings of the National Academy of Sciences of the United States of America,2009,106(31):13124−13129. doi: 10.1073/pnas.0900689106
[29]	袁扬静, 胡玉林, 谢江辉. 温度对采后香蕉果实糖代谢及其酶活性的影响[J]. 热带作物学报,2011,32(1):66−70. [YUAN Y J, HU Y L, XIE J H, et al. Effect of temperature on sugar metabolism and enzyme activities in postharvest ripening banana fruit[J]. Chinese Journal of Tropical Crops,2011,32(1):66−70. doi: 10.3969/j.issn.1000-2561.2011.01.013
[30]	李芋萱, 曾凯芳, 王宝刚, 等. 不同处理对采后果实糖代谢过程影响研究进展[J]. 食品科学,2015,36(5):283−288. [LI Y X, ZENG K F, WANG B G, et al. Advances in regulation of sugar metabolism in postharvest fruits by different treatments[J]. Food Science,2015,36(5):283−288. doi: 10.7506/spkx1002-6630-201505050
[31]	KAREN K. Sucrose metabolism: Regulatory mechanisms and pivotal roles in sugar sensing and plant development[J]. Current Opinion in Plant Biology,2004,7(3):235−246. doi: 10.1016/j.pbi.2004.03.014
[32]	魏长宾, 武红霞, 马蔚红, 等. 芒果成熟阶段蔗糖代谢及其相关酶类研究[J]. 西南农业学报,2008(4):972−974. [WEI C B, WU H X, MA W H, et al. Sucrose metabolism in Irwin mango (Mangifera indica L.) during maturation[J]. Southwest China Journal of Acultural Sciences,2008(4):972−974. doi: 10.3969/j.issn.1001-4829.2008.04.019
[33]	JIANG Y M, ZHANG Z Q, JOYCE D C, et al. Postharvest biology and handling of longan fruit (Dimocarpus longan Lour.)[J]. Postharvest Biology and Technology,2002,26(3):241−252. doi: 10.1016/S0925-5214(02)00047-9
[34]	高浩祥, 陈南, 徐乾达, 等. 不同保藏方式对龙眼贮藏过程中感官与理化品质的影响[J]. 食品工业,2018,39(10):137−141. [GAO H X, CHEN N, XU Q D, et al. The effect of different preservation methods on the sensory and chemical properties of longan during storage[J]. The Food Industry,2018,39(10):137−141.
[35]	孔祥佳, 林瑜, 林河通, 等. 低温贮藏对晚熟龙眼“立冬本”果实采后生理和品质的影响[J]. 包装与食品机械,2008(1):1−6. [KONG X J, LIN Y, LIN H T, et al. Effects of low temperature storage on post-harvest physiology and quality of late-matured 'Lidongben' longan fruit[J]. Packaging and Food Machinery,2008(1):1−6. doi: 10.3969/j.issn.1005-1295.2008.01.002

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