Effects of Carnosine Combined with Ultra High Pressure on the Lipid Oxidation and Myofibrillary Protein of Snakehead Fish

SUN Liangge; XU Jiamei; LV Jingxiu; ZHANG Xinxiao; XU Weimin; LI Pengpeng; WANG Daoying

doi:10.13386/j.issn1002-0306.2021060258

Volume 43 Issue 4

Feb. 2022

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Science and Technology of Food Industry > 2022 > 43(4): 114-122. > DOI: 10.13386/j.issn1002-0306.2021060258

SUN Liangge, XU Jiamei, LV Jingxiu, et al. Effects of Carnosine Combined with Ultra High Pressure on the Lipid Oxidation and Myofibrillary Protein of Snakehead Fish[J]. Science and Technology of Food Industry, 2022, 43(4): 114−122. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021060258.

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Effects of Carnosine Combined with Ultra High Pressure on the Lipid Oxidation and Myofibrillary Protein of Snakehead Fish

SUN Liangge^1,,
XU Jiamei^{1, 2},
LV Jingxiu^{1, 2},
ZHANG Xinxiao¹,
XU Weimin^{1, 2},
LI Pengpeng^{1, 2, ,},
WANG Daoying^{1, 2}

1.
Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Science, Nanjing 210014, China
2.
College of Food and Bioengineering, Jiangsu University, Zhenjiang 212013, China

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Received Date: June 28, 2021
Available Online: December 15, 2021

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Abstract

Abstract

The study aimed to investigate the effect of carnosine combined with ultra-high pressure (CUH) on the lipid oxidation and myofibrillar protein (MP) of snakehead fish meat during refrigerated storage. Studies on lipid and protein oxidation, protein degradation and protein structure were used to analyze the effect of CUH treatment (carnosine concentration 25 mmol/L, ultra-high pressure pressure 300 MPa, pressure holding time 900 s) on snakehead fish. The lipid oxidation of fish meat was analyzed by measuring the lipoxygenase activity and the change of MDA content in CUH-treated fish. The change in the carbonyl content of MP during refrigerated storage was used to characterize the degree of fish protein oxidation. SDS-PAGE, TCA-soluble peptides and total protease activity were used to determine the effect of CUH treatment on fish protein degradation. In order to further understand the effect of CUH treatment on the structure of snakehead fish meat and MP during refrigerated storage, SEM and CD tracking measurements were carried out on fish. The results showed that the lipoxygenase activity of fish meat treated with ultra-high pressure decreased by 64.46%. The inhibition rates of lipid oxidation and protein oxidation of fish treated with CUH were 51.25% and 3.63% on the 9th day of the refrigerated storage period, respectively. The total protease activity of fish after UHP treatment decreased by 25.75%. CUH treatment significantly reduced the degradation of MP during refrigerated storage (P<0.05). The protein degradation inhibition rate was 63.41% on the 9th day. The CUH-treated fish had complete and tight fibers during refrigerated storage. New hydrogen bonds were formed in the MP and the structure of proteins was more orderly arranged. In summary, CUH treatment could not only inhibit the lipid and protein oxidation and protein degradation of snakehead fish, but also maintain the structure of fish meat and MP. CUH treatment significantly improved the quality of snakehead fish and would provid a theoretical basis for improving the storage quality of black fish meat.
- snakehead fish,
- carnosine,
- ultra-high pressure,
- lipid oxidation,
- myofibrillar protein

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[1]	任丽琨, 李婷婷, 赫彬彬, 等. 黑鱼鱼片复合保鲜剂的选择及其保鲜效果[J]. 中国食品学报,2019,19(9):210−220. [REN Likun, LI Tingting, HE Binbin, et al. Selection of compound fresh-keeping agent for black fish fillet and its fresh-keeping effect[J]. Chinese Journal of Food Science,2019,19(9):210−220.
[2]	周蓓蓓, 胡王, 陈小雷, 等. 鱼制品腥味物质检测分析及去除技术研究进展[J]. 食品研究与开发,2016,37(14):185−192. [ZHOU Beibei, HU Wang, CHEN Xiaolei, et al. Research progress in detection, analysis and removal of fishy substances in fish products[J]. Food Research and Development,2016,37(14):185−192. doi: 10.3969/j.issn.1005-6521.2016.14.046
[3]	WANG Z M, FEI Z. Effects of malondialdehyde as a byproduct of lipid oxidation on protein oxidation in rabbit meat[J]. Food Chemistry,2019,288:405−412. doi: 10.1016/j.foodchem.2019.02.126
[4]	陈冲, 赵谋明, 孙为正. 含酪氨酸二肽在脂质体体系中的抗氧化活性与供电子能力[J]. 食品科学,2021,42(11):1−7. [CHEN Chong, ZHAO Mouming, SUN Weizheng. Antioxidant activity and electron donating ability of dipeptide containing tyrosine in liposome system[J]. Food Science,2021,42(11):1−7. doi: 10.7506/spkx1002-6630-20200617-232
[5]	邢子鑫, 朱秋劲. 肌肽对猪肉的氧化抑制和保鲜作用[J]. 肉类研究,2009(10):8−12. [XING Zixin, ZHU Qiujin. Oxidation inhibition and fresh-keeping effect of carnosine on pork[J]. Meat Research,2009(10):8−12. doi: 10.3969/j.issn.1001-8123.2009.10.005
[6]	王雨洁. 超高压处理鱼肉制品品质的影响综述[J]. 食品安全导刊,2020(33):182−182. [WANG Yujie. A review of the effects of ultra-high pressure treatment on the quality of fish products[J]. Food Safety Guide,2020(33):182−182.
[7]	BRUSCHI C, KOMORA N, CASTRO S M, et al. High hydrostatic pressure effects on Listeria monocytogenes andL. innocua: Evidence for variability in inactivation behaviour and in resistance to pediocin bac HA-6111-2[J]. Food Microbiology,2017,64:226−231. doi: 10.1016/j.fm.2017.01.011
[8]	SEVENICHR R C, KNORR D. A scientific and in-terdisciplinary approach for high pressure processing as a future toolbox for safe and high quality products: A review[J]. Innovative Food Science and Emerging Technologies,2016,38:65−75. doi: 10.1016/j.ifset.2016.09.013
[9]	BAJOVIC B, BOLUMAR T, HEINZ V. Quality considerations with high pressure processing of fresh and value added meat products[J]. Meat Science,2012,92(3):280−289. doi: 10.1016/j.meatsci.2012.04.024
[10]	GATA J L, PINTO M C, MACÍAS P. Lipoxygenase activity in pig muscle: purification and partial characterization[J]. Journal of Agricultural and Food Chemistry,1996,44(9):2573−2577. doi: 10.1021/jf960149n
[11]	SHI H B, ZHOU T, WANG X, et al. Effects of the structure and gel properties of myofibrillar protein on chicken breast quality treated with ultrasound-assisted potassium alginate[J]. Food Chemistry,2021,358(15):129873.
[12]	ZOU Y, BIAN H, LI P, et al. Optimization and physicochemical properties of nutritional protein isolatefrom pork liver with ultrasound-assisted alkaline extraction[J]. Animal Science Journal,2017,89(2):456−466.
[13]	WG A, XR A, YLB C, et al. The beneficial effects of grape seed, sage and oregano extracts on the quality and volatile flavor component of hairtail fish balls during cold storage at 4 ℃[J]. LWT,2019,101:25−31. doi: 10.1016/j.lwt.2018.11.024
[14]	GAVAN E, GOVERS S K, AERTSEN A. Impact of high hydrostatic pressure on bacterial proteostasis[J]. Biophysical Chemistry,2017,231:3−9. doi: 10.1016/j.bpc.2017.03.005
[15]	王帮国, 林琳, 余振宇, 等. 白鲢鱼肌肉脂肪氧合酶提取条件优化及酶学性质研究[J]. 食品工业科技,2017,38(9):113−118. [WANG Bangguo, LIN Lin, YU Zhenyu, et al. Optimization of extraction conditions of silver carp muscle lipoxygenase and study on enzymatic properties[J]. Science and Technology of Food Industry,2017,38(9):113−118.
[16]	GOPALAKRISHNAN J, DECKER E A, MEANS W J. Antioxidant activity of mechanically separated pork extracts[J]. Meat Science,1999,52(1):101−110. doi: 10.1016/S0309-1740(98)00154-5
[17]	李林. 微生物在发酵酸鱼中对脂质变化和风味组成的影响[D]. 无锡: 江南大学. LI Lin. Effects of microorganisms on lipid changes and flavor composition in fermented sour fish[D]. Wuxi: Jiangnan University, 2019.
[18]	MARCOS B, AYMERICH T, GARRIGA M, et al. Active packaging containing nisin and high pressure processing as post-processing listericidal treatments for convenience fermented sausages[J]. Food Control,2013,30(1):325−330. doi: 10.1016/j.foodcont.2012.07.019
[19]	SIMONIN H, DURANTON F, DE L M. New insights into the high-pressure processing of meat and meat products[J]. Comprehensive Reviews in Food Science and Food Safety,2012,11(3):285−306. doi: 10.1111/j.1541-4337.2012.00184.x
[20]	朱文慧, 宦海珍, 李月, 等. 蛋白氧化对秘鲁鱿鱼肌肉品质及其结构的影响[J]. 食品工业科技,2019,40(17):25−29,37. [ZHU Wenhui, HUAN Haizhen, LI Yue, et al. The effect of protein oxidation on the muscle quality and structure of Peruvian squid[J]. Food Industry Science and Technology,2019,40(17):25−29,37.
[21]	赵冰, 张顺亮, 李素, 等. 脂肪氧化对肌原纤维蛋白氧化及其结构和功能性质的影响[J]. 食品科学,2018,39(5):40−46. [ZHAO Bing, ZHANG Shunliang, LI Su, et al. The effect of fat oxidation on myofibril protein oxidation and its structural and functional properties[J]. Food Science,2018,39(5):40−46. doi: 10.7506/spkx1002-6630-201805007
[22]	HIPKISS A R, BROWNSON C, CARRIER M J. Carnosine, the anti-ageing, anti-oxidant dipeptide may react with carbonyl groups[J]. Mech Ageing Dev,2001,122(13):1431−1445. doi: 10.1016/S0047-6374(01)00272-X
[23]	马海建. 超高压处理对草鱼鱼肉和鱼糜制品品质的影响[D]. 上海: 上海海洋大学, 2016. MA Haijian. The effect of ultra-high pressure treatment on the quality of grass carp meat and surimi products[D]. Shanghai: Shanghai Ocean University, 2016.
[24]	叶凤凌, 周敏之, 池玉闽, 等. 蛋白氧化对肉品品质影响及其控制方法研究进展[J]. 食品工业科技,2020,41(15):315−322. [YE Fengling, ZHOU Minzhi, CHI Yumin, et al. The effect of protein oxidation on meat quality and its control methods[J]. Science and Technology of Food Industry,2020,41(15):315−322.
[25]	葛黎红. 内源蛋白酶在低温保鲜草鱼质构劣化中的作用与控制研究[D]. 无锡: 江南大学, 2017. GE Lihong. Study on the effect and control of endogenous protease in low-temperature preservation of grass carp texture degradation[D]. Wuxi: Jiangnan University, 2017.
[26]	闫春子. 超高压处理对淡水鱼冷藏保鲜效果的影响[D]. 无锡: 江南大学, 2016. YAN Chunzi. The effect of ultra-high pressure treatment on the freshwater preservation of freshwater fish[D]. Wuxi: Jiangnan University, 2016.
[27]	王敏, 李梦璐, 葛庆丰. 微生物外源酶对发酵肉制品品质的影响[J]. 食品与发酵工业,2012,38(10):134−139. [WANG Min, LI Menglu, GE Qingfeng. The effect of microbial exogenous enzymes on the quality of fermented meat products[J]. Food and Fermentation Industries,2012,38(10):134−139.
[28]	LAMETSCH R, ROEPSTORFF P, MØLLER H S, et al. Identification of myofibrillar substrates for μ-calpain[J]. Meat Science,2004,68(4):515−521. doi: 10.1016/j.meatsci.2004.03.018
[29]	GREENFIELD N J. Methods to estimate the conformation of proteins and polypeptides from circular dichroism data[J]. Analytical Biochemistry,1996,235(1):1−10. doi: 10.1006/abio.1996.0084
[30]	SREERAMA N, VENYAMINOV S Y, WOODY R W. Estimation of the number of Α helical and Β strand segments in proteins using circular dichroism spectroscopy[J]. Protein Science,1999,8(2):370−380.
[31]	叶凤凌, 池玉闽, 周敏之. 氧化对兔肉肌原纤维蛋白结构, 乳化性和凝胶性的影响研究[J]. 食品工业科技,2020,41(24):30−38. [YE Fengling, CHI Yumin, ZHOU Minzhi. The effect of oxidation on the structure, emulsification and gelation of rabbit muscle myofibril protein[J]. Science and Technology of Food Industry,2020,41(24):30−38.
[32]	ZWA B, JT C, HUI Z, et al. A comprehensive insight into the effects of microbial spoilage, myoglobin autoxidation, lipid oxidation, and protein oxidation on the discoloration of rabbit meat during retail display-ScienceDirect[J]. Meat Science,2020:172.
[33]	王玮, 葛毅强, 陈颖, 等. 超高压对高铁肌红蛋白还原酶活性及二级结构的影响[J]. 中国食品学报,2015,15(10):134−140. [WANG Wei, GE Yiqiang, CHEN Ying, et al. Effects of ultra-high pressure on the activity and secondary structure of metmyoglobin reductase[J]. Chinese Journal of Food Science,2015,15(10):134−140. doi: 10.16429/j.1009-7848.2015.10.018
[34]	TAN F J, LAI K M, HSU K C. A comparative study on physical properties and chemical interactions of gels from tilapia meat pastes induced by heat and pressure[J]. Journal of Texture Studies,2010,41(2):153−170. doi: 10.1111/j.1745-4603.2010.00219.x

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