Establishment of the Method of Immunoenrichment Combined with MALDI-TOF MS to Detect <i>Cronobacter sakazakii</i> in Milk Powder

ZHAO Hong; YANG Liu; ZHAO Liangjuan; PANG Lu; ZHANG Junzhe; ZHU Shaoying; ZHAO Yuling; TAN Youlan; ZHAO Huabing; LIU Pei; LIU Guohong; WU Lan; DONG Zhizhen

doi:10.13386/j.issn1002-0306.2021060253

Volume 43 Issue 5

Feb. 2022

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Science and Technology of Food Industry > 2022 > 43(5): 286-293. > DOI: 10.13386/j.issn1002-0306.2021060253

ZHAO Hong, YANG Liu, ZHAO Liangjuan, et al. Establishment of the Method of Immunoenrichment Combined with MALDI-TOF MS to Detect Cronobacter sakazakii in Milk Powder[J]. Science and Technology of Food Industry, 2022, 43(5): 286−293. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021060253.

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Establishment of the Method of Immunoenrichment Combined with MALDI-TOF MS to Detect Cronobacter sakazakii in Milk Powder

1.
Animal, Plant and Food Stuffs Inspection Center of Tianjin Customs, Tianjin 300461, China
2.
College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China
3.
Hohhot Customs District People’s Republic of China, Hohhot 010090, China
4.
Key Laboratory of Non-Traditional Security Risk Prevention and Control of Tianjin Port, Tianjin 300461, China

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Received Date: June 28, 2021
Available Online: January 04, 2022

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Abstract

Abstract

Objective: To prepare an efficient and specific antibody against Cronobacter sakazakii and its immunomagnetic beads, and establish a method of immunoenrichment combined with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) for the detection of Cronobacter sakazakii in milk powder. Methods: The antibody and its immune beads of Cronobacter sakazakii were prepared, the capture rate of immune magnetic beads was studied in pure culture and milk powder matrix. The specific of the beads were identified and verified by using MALDI-TOF MS. Results: The capture rate of four strains of Cronobacter sakazakii and its mixed bacteria in the pure culture and milk powder matrix were all over 80%, and the results of MALDI-TOF MS showed that the identification were better under different contamination conditions of different impurities in the milk powder matrix, even under the high proportion of (1:100) of the bacteria, it could be used to identify Cronobacter sakazakii in milk powder accurately, at this time, the concentration of Cronobacter sakazakii in the sample was only 20 CFU/mL. Conclusion: A simple and accurate method for the detection of Cronobacter sakazakii in milk powder by immunoenrichment and MALDI-TOF MS was established, which provided a new method reference for the rapid and accurate identification of Cronobacter sakazakii in milk powder.
- milk power,
- Cronobacter sakazakii,
- immunoenrichment,
- MALDI-TOF MS,
- detection

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