Effects of Microwave-assisted L-phenylalanine Treatment on the Flavonoids of Tartary Buckwheat during Germination

XU Xianmeng; BIAN Zixiu; WANG Shunmin; LU Ning; ZHANG Huimin; WANG Junzhen

doi:10.13386/j.issn1002-0306.2021060227

Volume 43 Issue 5

Feb. 2022

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Science and Technology of Food Industry > 2022 > 43(5): 191-198. > DOI: 10.13386/j.issn1002-0306.2021060227

XU Xianmeng, BIAN Zixiu, WANG Shunmin, et al. Effects of Microwave-assisted L-phenylalanine Treatment on the Flavonoids of Tartary Buckwheat during Germination[J]. Science and Technology of Food Industry, 2022, 43(5): 191−198. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021060227.

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Effects of Microwave-assisted L-phenylalanine Treatment on the Flavonoids of Tartary Buckwheat during Germination

1.
Department of Biotechnology and Food Engineering, Bozhou University, Bozhou 236800, China
2.
Bozhou Key Laboratory of Medicinal and Edible Homology Functional Food, Bozhou 236800, China
3.
College of Biological and Food Engineering, Anhui Polytechnic University, Wuhu 241000, China
4.
Xichang Agricultural Institute of Liangshan, Xichang 615000, China

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Received Date: June 28, 2021
Available Online: December 26, 2021

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Abstract

Abstract

In the study, tartary buckwheat sprouts of Chuanqiao 1 cultivar were investigated, and the effects of microwave-assisted L-phenylalanine (L-Phe) treatment on the flavonoids of tartary buckwheat during germination were studied. The content of flavonoids of tartary buckwheat sprouts was used as the standard, the single factor test and CCD response surface design methods were used to optimize the process of flavonoid enrichment by microwave-assisted L-Phe treatment of tartary buckwheat during germination. High performance liquid chromatography (HPLC) was used to analyze the flavonoid in tartary buckwheat sprouts. The results showed that the tartary buckwheat seeds were disinfected and germinated, then the seeds were treated by microwave with the power of 250 W for 90 s, the microwave temperature was (40±2)℃. Then, the tartary buckwheat seeds were cultured in the dark for 7 days at the condition of the temperature of 25 ℃ and the humidity of 75%±5% RH, and 2.90 mmol/L L-Phe solution was put into the germination tray as a nutrient solution. The result by detection showed the flavonoid content of tartary buckwheat sprouts on the 7th day was 6.26 g/100 g. Simultaneously, three flavonoids, chlorogenic acid, vitexin and rutin, were separated and determined by HPLC, and the content was 3.2764, 0.5644 and 31.8962 mg/g, respectively.
- tartary buckwheat sprouts,
- flavonoids enrichment,
- microwave,
- L-Phe,
- HPLC

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References

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