Effects of Microwave-assisted L-phenylalanine Treatment on the Flavonoids of Tartary Buckwheat during Germination

XU Xianmeng; BIAN Zixiu; WANG Shunmin; LU Ning; ZHANG Huimin; WANG Junzhen

doi:10.13386/j.issn1002-0306.2021060227

Volume 43 Issue 5

Feb. 2022

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Science and Technology of Food Industry > 2022 > 43(5): 191-198. > DOI: 10.13386/j.issn1002-0306.2021060227

XU Xianmeng, BIAN Zixiu, WANG Shunmin, et al. Effects of Microwave-assisted L-phenylalanine Treatment on the Flavonoids of Tartary Buckwheat during Germination[J]. Science and Technology of Food Industry, 2022, 43(5): 191−198. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021060227.

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Effects of Microwave-assisted L-phenylalanine Treatment on the Flavonoids of Tartary Buckwheat during Germination

1.
Department of Biotechnology and Food Engineering, Bozhou University, Bozhou 236800, China
2.
Bozhou Key Laboratory of Medicinal and Edible Homology Functional Food, Bozhou 236800, China
3.
College of Biological and Food Engineering, Anhui Polytechnic University, Wuhu 241000, China
4.
Xichang Agricultural Institute of Liangshan, Xichang 615000, China

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Received Date: June 28, 2021
Available Online: December 26, 2021

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Abstract

Abstract

In the study, tartary buckwheat sprouts of Chuanqiao 1 cultivar were investigated, and the effects of microwave-assisted L-phenylalanine (L-Phe) treatment on the flavonoids of tartary buckwheat during germination were studied. The content of flavonoids of tartary buckwheat sprouts was used as the standard, the single factor test and CCD response surface design methods were used to optimize the process of flavonoid enrichment by microwave-assisted L-Phe treatment of tartary buckwheat during germination. High performance liquid chromatography (HPLC) was used to analyze the flavonoid in tartary buckwheat sprouts. The results showed that the tartary buckwheat seeds were disinfected and germinated, then the seeds were treated by microwave with the power of 250 W for 90 s, the microwave temperature was (40±2)℃. Then, the tartary buckwheat seeds were cultured in the dark for 7 days at the condition of the temperature of 25 ℃ and the humidity of 75%±5% RH, and 2.90 mmol/L L-Phe solution was put into the germination tray as a nutrient solution. The result by detection showed the flavonoid content of tartary buckwheat sprouts on the 7th day was 6.26 g/100 g. Simultaneously, three flavonoids, chlorogenic acid, vitexin and rutin, were separated and determined by HPLC, and the content was 3.2764, 0.5644 and 31.8962 mg/g, respectively.
- tartary buckwheat sprouts,
- flavonoids enrichment,
- microwave,
- L-Phe,
- HPLC

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References

[1]	LEE L S, CHOI E J, KIM C H, et al. Contribution of flavonoids to the antioxidant properties of common and tartary buckwheat[J]. Journal of Cereal Science,2016,68(1):181−186.
[2]	江兰, 赵江林, 何小慧, 等. 海藻酸钠对苦荞芽生长和黄酮类物质富集的影响[J]. 食品研究与开发,2021,41(20):40−44. [JIANG L, ZHAO J L, HE X H, et al. Effects of sodium alginate on the sprout growth and the flavonoids enrichment of tartary buckwheat[J]. Food Research and Development,2021,41(20):40−44.
[3]	ZHOU J, LI C L, GAO F, et al. Characterization of three glucosyltransferase genes in tartary buckwheat and their expression after cold stress[J]. Journal of Agricultural & Food Chemistry,2016,64(37):6930−6938.
[4]	曾俊, 李梅, 刘晓爽, 等. 黑苦荞黄酮类化合物的提纯及抑菌活性研究[J]. 重庆师范大学学报(自然科学版),2020,37(6):126−130. [ZENG J, LI M, LIU X S. Sdudy on the purification and bacteriostatic activity of flavonoids from black tartary buckwheat[J]. Journal of Chongqing Normal University (Natural Science),2020,37(6):126−130.
[5]	FAN Z. Chemical composition and health effects of tartary buckwheat[J]. Food Chemistry,2016,203:231−245. doi: 10.1016/j.foodchem.2016.02.050
[6]	ZHOU Y, WANG H, CUI L, et al. Evolution of nutrient ingredients in tartary buckwheat seeds during germination[J]. Food Chemistry,2015,186(1):244−248.
[7]	THWE A A, KIM Y, LI X, et al. Accumulation of phenylpropanoids and correlated gene expression in hairy roots of tartary buckwheat under light and dark conditions[J]. Applied Biochemistry Biotechnology,2014,174(7):2537−2547. doi: 10.1007/s12010-014-1203-9
[8]	周小理, 方向, 周一鸣, 等. 磁场对苦荞种子萌发过程中黄酮类物质的诱导效应[J]. 食品科学,2012,33(21):20−23. [ZHOU X L, FANG X, ZHOU Y M, et al. Effect of magnetic field stimulation on flavonoid synthesis in tartary buckwheat (Fagopyrum tataricum Gaertn) sprouts[J]. Food Science,2012,33(21):20−23.
[9]	田月娥, 车志平, 刘圣明, 等. 微波处理对8种瓜类种子萌发的影响[J]. 贵州农业科学,2018,46(7):126−130. [TIAN Y E, CHE Z P, LIU S M, et al. Effect of microwave treatment on seed germination of eight kinds of gourds[J]. Guizhou Agricultural Sciences,2018,46(7):126−130. doi: 10.3969/j.issn.1001-3601.2018.07.032
[10]	黎兆安, 韦善富, 周祖富, 等. 微波辐射对水稻种子发芽势及生理生化特性的影响[J]. 中国种业,2010(1):46−48. [LI Z A, WEI S F, ZHOU Z F, et al. Microwave radiation on the germination and physiological biochemical characteristics of rice seed[J]. China Seed Industry,2010(1):46−48. doi: 10.3969/j.issn.1671-895X.2010.01.021
[11]	周丽艳, 马英华. 微波处理对几种蔬菜种子萌发的影响(简报)[J]. 河北农业技术师范学院学报,1998,12(1):66−67. [ZHOU L Y, MA Y H. The influence of microray on the germination of vegetable seeds (Bulletin)[J]. Journal of Hebei Agrotechnical Teachers,1998,12(1):66−67.
[12]	李波, 焦德志, 战春岩, 等. 微波处理苜蓿种子发芽率及其幼苗抗旱性的影响[J]. 种子,2006,25(12):28−30. [LI B, JIAO D Z, ZHAN C YL, etal. Influence of different microwave treatment on germination and seedling resistanced rought of alfalfa[J]. Seed,2006,25(12):28−30. doi: 10.3969/j.issn.1001-4705.2006.12.008
[13]	SHIVHARE U S, RAGHAVAN V. Microwave drying of soybean at 2.45 GHz[J]. Journal of Microwave Power and Electromagnetic Energy,1993,28(1):11−17. doi: 10.1080/08327823.1993.11688200
[14]	PAVE K, ALEXANDRA R, ANDREA H, et al. Processing of yellow pea by germination, microwave treatment and drying[J]. Innovative Food Science and Technologies,2001,2(2):133−177. doi: 10.1016/S1466-8564(01)00036-4
[15]	马辉, 卞紫秀, 陈雪怡, 等. 微波协同L-Phe处理对萌发苦荞中主要营养成分的影响[J]. 安徽工程大学学报,2019,34(6):1−7. [MA H, BIAN Z X, CHEN X Y, et al. Effects of microwave assisted L-Phe treatment on main nutrients of tartary buckwheat during germination[J]. Journal of Anhui Polytechnic University,2019,34(6):1−7. doi: 10.3969/j.issn.2095-0977.2019.06.001
[16]	PELLETIER M K, BURBULIS I E, WINKELSHIRLEY B, et al. Disruption of specific flavonoid genes enhances the accumulation of flavonoid enzymes and end-products in Arabidopsis seedlings[J]. Plant Molecular Biology,1999,40(1):45−54. doi: 10.1023/A:1026414301100
[17]	SEO J M, ARASU M V, KIM Y B, et al. Phenylalanine and LED lights enhance phenolic compound production in tartary buckwheat sprouts[J]. Food Chemistry,2015,177(1):204−213.
[18]	赵强, 李惠, 李文婕, 等. 干旱和盐胁迫对苦荞种子萌发及生长特性的影响[J]. 天水师范学院学报,2020,40(5):72−77. [ZHAO Q, LI H, LI W J, et al. The comparative study of heavy metal salt stress for tartary buckwheat at seed germination and growth characteristics[J]. Journal of Tianshui Normal University,2020,40(5):72−77. doi: 10.3969/j.issn.1671-1351.2020.05.019
[19]	JI H, WEN T, ZHOU X, et al. Combined effects of blue and ultraviolet lights on the accumulation of flavonoids in tartary buckwheat sprouts[J]. Polish Journal of Food & Nutrition Sciences,2016,66(2):93−98.
[20]	宋越冬, 陈晓庆, 张毓敏, 等. 荞麦叶黄酮的提取工艺优化及其抗氧化性[J]. 食品工业科技,2021,42(7):180−187. [SONG Y D, CHEN X Q, ZHANG Y M, et al. Optimization of extraction process of flavonoids from Fagopy rumesculentum Moench leaves and its antioxidant properties[J]. Science and Technology of Food Industry,2021,42(7):180−187.
[21]	MA H, XU X M, WANG S M, et al. Effects of microwave irradiation on the expression of key flavonoid biosynthetic enzyme genes and the accumulation of flavonoid products in Fagopyrum tataricum sprouts[J]. Journal of Cereal Science,2021,101:103275. doi: 10.1016/j.jcs.2021.103275
[22]	RANDHIR R, SHETTY K. Microwave-induced stimulation of L-DOPA, phenolics and antioxidant activity in fava bean (Vicia faba) for Parkinson's diet[J]. Process Biochemistry,2004,39(11):1775−1784. doi: 10.1016/j.procbio.2003.08.006
[23]	王顺民, 卞紫秀, 汪建飞, 等. 微波处理对豌豆种子萌发及芽苗中营养成分的影响[J]. 干旱地区农业研究,2018,36(5):40−46. [WANG S M, BIAN Z X, WANG J F, et al. Effects of microwave enhancement on pea seeds germination and production of nutrient in sprouts[J]. Agricultural Research in the Arid Areas,2018,36(5):40−46. doi: 10.7606/j.issn.1000-7601.2018.05.07
[24]	KWON Y, LEE K, YUN T, et al. Effect of heat pretreatment on the functional constituents of rice germ[J]. Preventive Nutrition and Food Science,2004,9(4):330−335. doi: 10.3746/jfn.2004.9.4.330
[25]	HITHAMANI G, SRINIVASAN K. Bioaccessibility of polyphenols from wheat (Triticum aestivum), sorghum (Sorghum bicolor), green gram (Vigna radiata), and chickpea (Cicer arietinum) as influenced by domestic food processing[J]. Journal of Agricultural and Food Chemistry,2014,62(46):11170−11179. doi: 10.1021/jf503450u
[26]	鲍雪纤, 郑书绿, 李莲芳, 等. 微波辐射和激素浸种对滇青冈种子发芽及幼苗生长的影响[J]. 种子,2015,34(7):12−15,19. [BAO X X, ZHENG S L, LI L F, et al. Effects of microwave radiation and hormone presoaking seed on germination and seedling growth of Cyclobalanopsis glaucoides[J]. Seed,2015,34(7):12−15,19.
[27]	胡燕月, 徐鹰, 潘金忠, 等. 微波处理对水稻种子萌发的影响(简报)[J]. 植物生理学通讯,1994,30(6):414−416. [HU Y Y, XU Y, PAN J Z, et al. Effect of microwave treatment on the germination of rice seeds[J]. Plant Physiology Communications,1994,30(6):414−416.
[28]	李一帆, 江莎, 杨俊红, 等. 微波处理对刺槐种子萌发特性的影响[J]. 内蒙古农业大学学报,2006,27(2):144−146. [LI Y F, JIANG S, YANG J H, et al. Effect of microwave on seed germination of Robinia pseudoacacia L J]. Journal of Inner Mongolia Agricultural University,2006,27(2):144−146.
[29]	陈梦洁, 吕长平, 孙田娇, 等. 微波辐射对‘凤丹’种子萌发及幼苗生长的影响[J]. 经济林研究,2021,39(2):238−243. [CHEN M J, LVU C P, SUN T J, et al. Effect of microwave radiation on seed germination and seedling growth of Peaonia ostii ‘Feng Dan’[J]. Non-wood Forest Research,2021,39(2):238−243.
[30]	张蕊思, 施宠, 安沙舟, 等. 微波辐射对冰草种子萌发及幼苗生理特性的影响[J]. 中国草地学报,2015,37(5):69−74. [ZHANG R S, SHI C, AN S Z, et al. Effect of microwave treatment on seed germination and seedling physiological characteristics of Agropyron cristatum[J]. Chinese Journal of Grassland,2015,37(5):69−74. doi: 10.3969/j.issn.1673-5021.2015.05.012