Study on Purification Process and Antioxidative Activity and <i>α</i>-Glucosidas Inhibitory Activity of Proanthocyanidins<italic/> from<i> Tetrastigma hemsleyanum</i>

HE Zhigui; XU Xianglin; CUI Yingying; WU Lan; ZHOU Xiaoyu; HUANG Zongfang; DU Miying

doi:10.13386/j.issn1002-0306.2021060194

Volume 43 Issue 6

Mar. 2022

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Science and Technology of Food Industry > 2022 > 43(6): 178-185. > DOI: 10.13386/j.issn1002-0306.2021060194

HE Zhigui, XU Xianglin, CUI Yingying, et al. Study on Purification Process and Antioxidative Activity and α-Glucosidas Inhibitory Activity of Proanthocyanidins from Tetrastigma hemsleyanum[J]. Science and Technology of Food Industry, 2022, 43(6): 178−185. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021060194.

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Study on Purification Process and Antioxidative Activity and α-Glucosidas Inhibitory Activity of Proanthocyanidins from Tetrastigma hemsleyanum

School of Leisure and Health, Guilin Tourism Univercity, Guilin 541006, China

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Received Date: June 22, 2021
Available Online: January 16, 2022

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Abstract

Abstract

In this paper, the purification process and biological activity of proanthocyanidins isolated from Tetrastigma hemsleyanum were explored to prepare economical and efficient proanthocyanidins. The static adsorption and desorption capacity of proanthocyanidins on five kinds of resins were compared. The dynamic adsorption and desorption conditions of resins were optimized. The effects of ethanol elution on the purity and biological activity of proanthocyanidins were evaluated. The results showed that AB-8 resin was chosen as the better sorbent for the purification of proanthocyanidins from Tetrastigma hemsleyanum. The optimal conditions for purifying proanthocyanidins were as follows: Sample concentration 6.00 mg/mL, loading rate 2 BV/h, loading amount 11 BV, eluent flow rate 1.5 BV/h, and eluent volume 2 BV. 70% ethnol had the best effect for the purification of proanthocyanidins, and the purification of proanthocyanidins were 97.31%±0.96%, 2.2 times the purification of crude extract. The DPPH scavenging activity, ABTS⁺ scavenging activity and α-glucosidase inhibitory activity were also increased dramatically and there was a significant positive correlation between these activities and content of proanthocyanidins（P<0.01）. Therefore, the AB-8 resin purification method was simple and efficient, and the Tetrastigma hemsleyanum proanthocyanidin had strong in vitro antioxidant activity and α-glucosidase inhibitory activity. It would provide a scientific reference for the comprehensive development and utilization of proanthocyanidins in Tetrastigma hemsleyanum.
- T. hemsleyanum Diels et Gilg proanthocyanidins,
- AB-8 resins,
- antioxidant activity,
- α-glucosidase suppression

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[1]	范世明, 徐惠龙, 谢心月, 等. 三叶青叶指纹图谱研究及8种酚类成分含量测定[J]. 中国中药杂志,2016,41(21):3975−3981. [FAN S M, XU H L, XIE X Y, et al. Study on UHPLC fingerprint and determination of eight phenolic components of Tetrastigma hemsleyanum leaves[J]. Chinese Journal of Chinese Materia Media,2016,41(21):3975−3981.
[2]	王满生, 杨晶, 王延周. 莲房原花青素提取纯化及其抗氧化活性研究进展[J]. 食品科技,2020,45(8):174−179. [WANG M S, YANG J, WANG Y Z. Research progress on extraction, purification and antioxidant activities of lotus seedpod procyanidins[J]. Food Science and Technology,2020,45(8):174−179.
[3]	DOWNING L E, FERGUSON B S, RODRIGUEZ K, et al. A grape seed procyanidin extract inhibits HDAC activity leading to increased Pparα phosphorylation and target-gene expression[J]. Molecular Nutrition & Food Research,2017,61(2):1600347.
[4]	郝小燕, 牟春堂, 乔栋, 等. 葡萄籽原花青素对羔羊瘤胃发酵、血清炎症及抗氧化指标的影响[J]. 中国农业科学,2021,54(10):2239−2248. [HAO X Y, MU C T, QIAO D, et al. Effects of high-concentrate diet supplemented with grape seed proanthocyanidins on rumen fermentation, inflammatory and antioxidant indicators of rumen and serum in lambs[J]. Scientia Agricultura Sinica,2021,54(10):2239−2248. doi: 10.3864/j.issn.0578-1752.2021.10.019
[5]	VALLS R M, LLAURADÓ E, FERNÁNDEZ-CASTILLEJO S, et al. Effects of low molecular weight procyanidin rich extract from french maritime pine bark on cardiovascular disease risk factors in stage-1 hypertensive subjects: Randomized, double-blind, crossover, placebo-controlled intervention trial[J]. Phytomedicine: International Journal of Phytotherapy and Phytopharmacology,2016,23(12):1451−1461. doi: 10.1016/j.phymed.2016.08.007
[6]	RONG S, HU X, ZHAO S, et al. Procyanidins extracted from the litchi pericarp ameliorate atherosclerosis in ApoE knockout mice: their effects on nitric oxide bioavailability and oxidative stress[J]. Food & Function, 2017, 8(11):4210-4216.
[7]	谢海, 战同霞, 钟明强. 原花青素对老年痴呆患者脑功能的影响[J]. 中国误诊学杂志,2008,8(24):5866−5867. [XIE H, ZHAN T X, ZHONG M Q. The effect of proanthocyanidins on brain function of patients with senile dementia[J]. Chinese Journal of Misdiagnostics,2008,8(24):5866−5867. doi: 10.3969/j.issn.1009-6647.2008.24.061
[8]	HELLSTROM J K, TORRONEN A R, MATTILA P H. Proanthocyanidins in common food products of plant origin[J]. Journal of Agricultural & Food Chemistry,2009,57(17):7899−7906.
[9]	张朝, 唐志书, 刘红波. 沙棘原花青素的提取、纯化及药理学研究进展[J]. 中南药学,2017,15(7):943−946. [ZHANG Z, TANG Z S, LIU H B. Extraction, purification and pharmacology progress of proanthocyanidins from Hippophae rhamnoidos Linn[J]. Central South Pharmacy,2017,15(7):943−946. doi: 10.7539/j.issn.1672-2981.2017.07.020
[10]	ZHANG B B, YANG H F, CHEN M S, et al. Microwave and enzyme-assisted extraction of grape seed procyanidins and study of their antibacterial activity[J]. Modern Food ence and Technology,2017,33(8):146−154.
[11]	ZHOU H C, TAN N F, LIN Y M, et al. Relationships between degree of polymerization and antioxidant activities: A Study on proanthocyanidins from the leaves of a medicinal mangrove plant Ceriops tagal[J]. PLoS One,2014,9(10):e107606. doi: 10.1371/journal.pone.0107606
[12]	BODOIRA R, ROSSI Y, MONTENEGRO M, et al. Extraction of antioxidant polyphenolic compounds from peanut skin using water-ethanol at high pressure and temperature conditions[J]. Journal of Supercritical Fluids,2017:57−65.
[13]	Cyboran-Mikołajczyk S, Csonka Ákos, Molnar J, et al. In vitro studies of anti-hemolytic and cytotoxic activity of procyanidin-rich extract from the leaves of Actinidia arguta[J]. Polish Journal of Food and Nutrition Sciences,2017,68(2):171−177.
[14]	LIU D D, CAO G, HAN L K, et al. Flavonoids from Radix Tetrastigmae inhibit TLR4/MD-2 mediated JNK and NF-kappaB pathway with anti-inflammatory properties[J]. Cytokine,2016,84:29−36. doi: 10.1016/j.cyto.2015.08.003
[15]	SUN Y, LI H Y, HU J, et al. Qualitative and quantitative analysis of phenolics in Tetrastigma hemsleyanum and their antioxidant and antiproliferative activities[J]. Journal of Agricultural Food Chemistry,2013,61(44):10507−10515. doi: 10.1021/jf4037547
[16]	许文, 傅志勤, 林婧, 等. HPLC-Q-TOF-MS和UPLC-QqQ-MS的三叶青主要成分定性与定量研究[J]. 中国中药杂志,2014,39(22):4365. [XU W, BO Z Q, LIN J, et al. Qualitative and quantitative analysis of major constituents in Tetrastigma hemsleyanum by HPLC-Q-TOF-MS and UPLC-QqQ-MS[J]. Chinese Journal of Chinese Materia Media,2014,39(22):4365.
[17]	李鑫, 刘景玲, 李彦, 等. 三叶青原花青素结构分析[J]. 食品科学,2018,39(16):247−255. [LI X, LIU J L, LI Y, et al. Structural analysis of proanthocyanidins from Tetrastigma hemsleyanum Diels et Gilg[J]. Food Science,2018,39(16):247−255. doi: 10.7506/spkx1002-6630-201816036
[18]	赵文娟, 宋扬, 杨洪江. 大孔吸附树脂纯化黑果枸杞中的原花青素[J]. 食品工业科技,2017,38(22):189−194. [ZHAO W J, SONG Y, YANG H J. Purification of the proanthocyanidins from Lycium ruthenicum Murr. by macroporous resin[J]. Science and Technology of Food Industry,2017,38(22):189−194.
[19]	褚仕超, 张宏, 邓莉, 等. 蚕豆皮原花青素纯化工艺优化[J]. 食品科技,2013,38(9):174−178,184. [CHU S C, ZHANG H, DENG L, et al. Optimization purifi cation of proanthocyanidins from Faba bean episperm[J]. Food Science and Technology,2013,38(9):174−178,184.
[20]	滕飞, 李丽, 皮子凤. 黑果腺肋花楸原花青素分离纯化工艺研究[J]. 长春师范大学学报,2019,38(4):67−72. [TENG F, LI L, PI Z F. Study on extraction process and purification conditions of proanthocyanidins from Aronia melanocarpa Elliot[J]. Journal of Changchun Normal University,2019,38(4):67−72.
[21]	何志贵, 聂相珍, 惠小涵, 等. 超声波辅助法提取三叶青原花青素工艺的研究[J]. 西北林学院学报,2019,34(3):207−211. [HE Z G, NIE X Z, HUI X H, et al. Ultrasonic-assisted extraction of proanthocyanidins from Tetrastigma hemsleyanum[J]. Journal of Northwest Forestry University,2019,34(3):207−211. doi: 10.3969/j.issn.1001-7461.2019.03.33
[22]	DONG Y, ZHAO M, SUN-WATERHOUSE D, et al. Absorption and desorption behaviour of the flavonoids from Glycyrrhiza glabra L. leaf on macroporous adsorption resins[J]. Food Chemistry,2015,168:538−545. doi: 10.1016/j.foodchem.2014.07.109
[23]	李彦, 李鑫, 刘景玲, 等. 鸡血藤原花青素的纯化及活性评价[J]. 食品科学,2016,37(17):45−51. [LI Y, LI X, LIU J L, et al. Purification and bioactivity evaluation of proanthocyanidins from Spatholobi Caulis[J]. Food Science,2016,37(17):45−51. doi: 10.7506/spkx1002-6630-201617008
[24]	LÓPEZ-ANGULO G, MONTES-AVILA J, SÁNCHEZ-XIMELLO L, et al. Anthocyanins of Pithecellobium dulce (Roxb.) Benth. fruit associated with high antioxidant and α-glucosidase inhibitory activities[J]. Plant Foods for Human Nutrition,2018(73):308−313.
[25]	赵平, 张月萍, 任鹏. AB-8大孔树脂对葡萄籽原花青素的吸附过程[J]. 化工学报,2013,64(3):980−985. [ZHAO P, ZHANG Y P, REN P. Adsorption process of proanthocyanidins in AB-8 macroporous resin[J]. CIESC Journa,2013,64(3):980−985. doi: 10.3969/j.issn.0438-1157.2013.03.027
[26]	马小琴, 王晓敏, 周凡. 大孔吸附树脂法分离纯化葡萄籽中原花青素[J]. 食品研究与开发,2017,38(16):54−57. [MA X Q, WANG X M, ZHOU F. Separation and purification of procyanidins in grape seed with macroporous adsorption resin[J]. Food Research and Development,2017,38(16):54−57. doi: 10.3969/j.issn.1005-6521.2017.16.012
[27]	蒋德旗, 李慧斌, 蓝雪叶, 等. AB-8树脂吸附分离苹果原花青素的研究[J]. 饲料工业,2014,35(3):18−20. [JIANG D Q, LI H B, LAN X Y, et al. Study on the adsorption and separation of procyanidins from apple with macroporous resin AB-8[J]. Feed Industry,2014,35(3):18−20.
[28]	刘景玲, 李匡元, 陈惜燕, 等. 密蒙花黄酮的纯化及抗氧化活性研究[J]. 食品研究与开发,2018,39(7):52−59. [LIU J L, LI K Y, CHEN X Y, et al. Purification and antioxidant ability evaluation of flavonoids from Buddleiae flos[J]. Food Research and Development,2018,39(7):52−59. doi: 10.3969/j.issn.1005-6521.2018.07.009
[29]	杨志娟, 曾真, 吴晓萍. 火龙果皮原花青素提取纯化及定性分析[J]. 食品科学,2015(2):75−79. [YANG Z J, ZENG Z, WU X P. Extraction, purification and qualitative analysis of proanthocyanidins from pitaya peel[J]. Food Science,2015(2):75−79. doi: 10.7506/spkx1002-6630-201502014
[30]	熊双丽, 卢飞, 史敏娟, 等. DPPH自由基清除活性评价方法在抗氧化剂筛选中的研究进展[J]. 食品工业科技,2012,33(8):380−383. [XIONG S L, LU F, SHI M J, et al. Advanement of evaluation methods about DPPH radical scavenging activity in screening antioxidant[J]. Science and Technology of Food Industry,2012,33(8):380−383.
[31]	李三省, 许丹丹, 张富粟, 等. AB-8大孔树脂纯化黑果枸杞原花青素及其稳定性研究[J]. 中国食品添加剂,2018(3):106−114. [LI S X, X D D, ZHANG F L, et al. Purification of proanthocyanidins from Lycium ruthenicum Murr. by AB-8 macroporous resin and its stability[J]. China Food Additives,2018(3):106−114. doi: 10.3969/j.issn.1006-2513.2018.03.009
[32]	浦娜娜. 白刺果原花青素的提取、纯化及抗氧化活性研究[D]. 济南: 齐鲁工业大学, 2019. PU N N. Study of Extraction, purification and antioxidant activity of proanthocyanidins from Nitraria[D]. Jinan: Qilu University of Technology, 2019.
[33]	DONG H Q, LI M, ZHU F, et al. Inhibitory potential of trilobatin from Lithocarpus polystachyus Rehd against α-glucosidase and α-amylase linked to type 2 diabetes[J]. Food Chemistry,2012,130(2):261−266. doi: 10.1016/j.foodchem.2011.07.030
[34]	SCHFER A, HGGER P. Oligomeric procyanidins of French maritime pine bark extract (Pycnogenol) effectively inhibit α-glucosidase[J]. Diabetes Research and Clinical Practice,2007,77(1):41−46. doi: 10.1016/j.diabres.2006.10.011

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