Identification of Characteristic Peptides of Different Gelatins in Donkey-hide Gelatin Lump by High Performance Liquid Chromatography-High Resolution Mass Spectrometry

SHA Xiaomei; ZHANG Lijun; JIANG Wenli; WANG Guangyao; XIE Zuohua; HUANG Guotai; TU Zongcai

doi:10.13386/j.issn1002-0306.2021060180

Volume 43 Issue 4

Feb. 2022

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Science and Technology of Food Industry > 2022 > 43(4): 97-104. > DOI: 10.13386/j.issn1002-0306.2021060180

SHA Xiaomei, ZHANG Lijun, JIANG Wenli, et al. Identification of Characteristic Peptides of Different Gelatins in Donkey-hide Gelatin Lump by High Performance Liquid Chromatography-High Resolution Mass Spectrometry[J]. Science and Technology of Food Industry, 2022, 43(4): 97−104. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021060180.

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Identification of Characteristic Peptides of Different Gelatins in Donkey-hide Gelatin Lump by High Performance Liquid Chromatography-High Resolution Mass Spectrometry

1.
National R & D Center for Freshwater Fish Processing, College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China
2.
Jiangxi Deshang Pharmaceutical Co., Ltd., Zhangshu 331208, China
3.
Jiangxi Deshang Pharmaceutical Research Institute Co., Ltd., Zhangshu 331208, China
4.
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China

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Received Date: June 20, 2021
Available Online: December 17, 2021

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Abstract

Abstract

To study the source of different gelatins of donkey-hide gelatin lumps, four kinds of donkey-hide gelatin lumps were added with low content (1 mg/mL) of bovine gelatin and porcine gelatin respectively. High-performance liquid chromatography-mass spectrometry (HPLC-MS/MS) were chosen to identify the characteristic peptides of donkey-hide gelatin, bovine gelatin and porcine gelatin. The results showed that 18, 3 and 5 common characteristic peptides were accurately detected in the four kinds of donkey-hide gelatin lump for donkey-hide gelatin, bovine gelatin and porcine gelatin, respectively, with 10, 1 and 1 from the α₁ chain and 8, 2 and 4 from the α₂ chain. Meanwhile, the number of common characteristic peptides with hydroxylated modifications was 15, 2 and 4 respectively, while those without any modification groups were 3, 1 and 1. These common characteristic peptides would provide a basis for the HPLC-MS/MS traceable identification of the gelatin source in donkey-hide gelatin lump, which is of reference significance for the quality assurance of donkey-hide gelatin lump products.

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References

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