SUN Meiling, QIU Xuezhi, ZHOU Jing, et al. Optimization of Extraction Process, Structural Characterization and Component Analysis of Total Flavonoids from Mesembryanthemum crystallinum[J]. Science and Technology of Food Industry, 2022, 43(4): 196−204. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021060123.
Citation: SUN Meiling, QIU Xuezhi, ZHOU Jing, et al. Optimization of Extraction Process, Structural Characterization and Component Analysis of Total Flavonoids from Mesembryanthemum crystallinum[J]. Science and Technology of Food Industry, 2022, 43(4): 196−204. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021060123.

Optimization of Extraction Process, Structural Characterization and Component Analysis of Total Flavonoids from Mesembryanthemum crystallinum

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  • Received Date: June 16, 2021
  • Available Online: December 17, 2021
  • In this study, the ultrasound-assisted extraction conditions of total flavonoids were optimized, and the structure and composition of total flavonoids were further analyzed. On the basis of single factor experiments, orthogonal experiments was carried out on four factors, including solid-liquid ratio, extraction time, temperature and power to obtain the optimal extraction conditions based on extraction yield. Under these conditions, total flavonoids were purified by D101 macroporous resin. The structure of total flavonoids before and after purification was characterized by UV-Vis and Fourier transform infrared spectroscopy (FT-IR). Finally, the constituents of the purified compounds were analyzed by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). The results showed that the optimal extraction conditions were as follows: Ethanol concentration of 60%, solid-liquid ratio of 1:25 mg/mL, ultrasonic temperature of 45 ℃, time of 120 min, power of 250 W, and extraction yield of 2.776%. The characteristic structure of total flavonoids was analyzed by UV-Vis spectroscopy (UV-Vis). Fourier transform infrared spectroscopy (FT-IR) also showed that the total flavonoids contained O-H, C-H, C=O, C=C, phenolic hydroxyl groups and other characteristic functional groups, which was consistent with the typical structure of flavonoids. Thirty flavonoids were detected in the purified products by UPLC-MS/MS, including citrinin, hesperetin, azalea, protocatechualdehyde, diosmin, naringin chalcone, sweet orange flavone, naringin and rutin. The relative content of citrinin was the highest, which was 50.854%±0.089% . This study would provide a theoretical basis for the functional development and utilization of crystal Mesembryanthemum crystallinum.
  • [1]
    张洪磊, 刘孟霞. 冰菜特征特性及控盐高产栽培技术[J]. 陕西农业科学,2015(3):122. [ZHANG H L, LIU M X. Characteristics and cultivation techniques of salt control and high yield of Mesembryanthemum crystallinum[J]. Shaanxi Journal of Agricultural Sciences,2015(3):122. doi: 10.3969/j.issn.0488-5368.2015.03.041
    [2]
    祁永琼, 王莉丽, 彭健. 冰菜大棚栽培技术[J]. 吉林农业,2016(21):98. [QI Y Q, WANG L L, PENG J. Cultivation technology of Mesembryanthemum crystallinum[J]. Agriculture of Jilin,2016(21):98.
    [3]
    于丽艳. 非洲冰菜高效栽培技术[J]. 北方园艺,2016(17):62−63. [YU L Y. The high-yield cultivation technique of African Mesembryanthemum crystallinum L[J]. Northern Horticulture,2016(17):62−63.
    [4]
    练冬梅, 姚运法, 赖正锋, 等. 冰菜主要营养成分及抗氧化活性分析[J]. 农业科学, 2020, 10(5): 225−230.

    LIAN D M, YAO Y F, LAI Z F, et al. Analysis of main nutrients and antioxidant activity of Mesembryanthemum crystallinum Linn. Hans Journal of Agricultural Sciences, 2020, 10(5): 225−230.
    [5]
    YOU X W, YIN S J, SUO F Y, et al. Biochar and fertilizer improved the growth and quality of the ice plant (Mesembryanthemum crystallinum L. ) shoots in a coastal soil of Yellow River Delta, China[J]. Science of the Total Environment,2021:775.
    [6]
    KONG W W, ZHU D, NOBLE J D, et al. Molecular changes in Mesembryanthemum crystallinum guard cells underlying the C3 to CAM transition. [J]. Plant Molecular Biology, 2020, 103(6): doi: 10.1007/s11103-020-01016-9.
    [7]
    王远, 郑雯, 袁田青, 等. 辣木叶总黄酮微波辅助提取工艺优化及其抑制α-葡萄糖苷酶活性研究[J]. 核农学报,2018,32(1):84−94. [WANG Y, ZHANG W, YUAN T Q, et al. Optimization of microwave-assisted extraction process of total flavonoids from Moringa oleifera Lam. leaves and its inhibition on α-glucosidase activity[J]. Journal of Nuclear Agricultural Sciences,2018,32(1):84−94. doi: 10.11869/j.issn.100-8551.2018.01.0084
    [8]
    李晓雪. 紫苏黄酮的提取工艺优化、含量分析及降糖活性研究[D]. 哈尔滨: 东北林业大学, 2020.

    LI X X. Study on extraction technology optimization, content analysis and hypoglycemic activity of flavonoids from Perilla[D]. Harbin: Northeast Forestry University, 2020.
    [9]
    冯艺飞. 茵陈黄酮提取纯化及特性研究[D]. 天津: 天津科技大学, 2019.

    FENG Y F. Study on extraction, purification and characteristics of flavonoids from Artemisia capillaris[D]. Tianjin: Tianjin University of Science and Technology, 2019.
    [10]
    刘亚倩, 魏华, 李贵, 等. 荠菜总黄酮质量分数测定及超声提取工艺优化[J]. 吉首大学学报(自然科学版),2016,37(1):74−79. [LIU Y Q, WEI H, LI G, et al. Content determination and ultrasonic extraction techniques of total flavonoids from Capsellae bursa-pastoris[J]. Journal of Jishou University(Natural Sciences Edition),2016,37(1):74−79.
    [11]
    荆常亮. 紫花苜蓿总黄酮的提取、纯化及其抗氧化活性研究[D]. 北京: 中国农业科学院, 2016.

    JING C L. Extraction, purification and antioxidant activity of total flavonoids from alfalfa [D]. Beijing: Chinese Academy of Agricultural Sciences, 2016.
    [12]
    孙美玲, 冯晓光, 侯雪飞, 等. 水晶冰菜总黄酮大孔树脂纯化工艺及体外降糖活性研究[J/OL]. 河南农业大学学报: 1−12 [2021-05-30]. https: //doi. org/10.16445/j. cnki. 1000-2340.20210521. 001.

    SUN M L, FENG X G, HOU X F, et al. Purification technology and in vitro hypoglycemic activity of total flavonoids macroporous resins from Mesembryanthemum crystallinum[J/OL]. Journal of Henan Agricultural University: 1−12 [2021-05-30].https://doi.org/10.16445/j.cnki. 000-2340. 20210521. 001.
    [13]
    李侠, 臧学丽, 徐祎博, 等. AB-8大孔树脂纯化绿豆皮黄酮工艺优化及纯化前后抗氧化能力比较[J]. 食品科学,2018,39(10):283−290. [LI X, ZANG X L, XU Y B, et al. Optimization of purification of flavonoids from Mung bean coat by AB-8 macroporous resin and comparison of antioxidant capacity of crude and purified flavonoids[J]. Food Science,2018,39(10):283−290. doi: 10.7506/spkx1002-6630-201810043
    [14]
    陈代秀, 夏伟丽, 胡思前, 等. 刺梨中活性物质的提取与性能探讨[J]. 化肥设计,2020,58(2):13−17. [CHEN D X, XIA W L, HU S Q, et al. Extraction of active substances from roxburgh rose and discussion on its properties[J]. Chemical Fertilizer Design,2020,58(2):13−17. doi: 10.3969/j.issn.1004-8901.2020.02.004
    [15]
    许玲玉, 陆兰宁, 陈冉, 等. 超声辅助提取牛角瓜中多酚工艺的研究[J]. 中国食品添加剂,2020,31(9):28−32. [XU L Y, LU L N, CHEN R, et al. Ultrasound-assisted extraction of polyphenols fromCalotropis gigantea[J]. China Food Additives,2020,31(9):28−32.
    [16]
    千春录, 侯顺超, 殷健东, 等. 响应面试验优化水芹黄酮超声波辅助提取工艺及其抗氧化性[J]. 食品科学,2016,37(10):76−81. [QIAN C L, HOU S C, YIN J D, et al. Optimization of ultrasonic-assisted extraction of flavonoids from Oenanthe javanica and there antioxidant activity[J]. Food Science,2016,37(10):76−81.
    [17]
    秦晶晶, 钱慧琴, 赵媛, 等. 柿叶总黄酮提取工艺优化及其抗氧化活性[J]. 食品工业科技,2020,41(13):32−38,45. [QIN J J, QIAN H Q, ZHAO Y, et al. Optimization of the extraction technology of total flavonoids and in vitro antioxidant activity of extract from persimmon leaves[J]. Science and Technology of Food Industry,2020,41(13):32−38,45.
    [18]
    陈红梅, 谢翎. 响应面法优化半枝莲黄酮提取工艺及体外抗氧化性分析[J]. 食品科学,2016,37(2):45−50. [CHEN H M, XIE L. Optimization of extraction process for flavonoid from Soutellaria barbata by response surface methodology and evaluation of its antioxidant activity[J]. Food Science,2016,37(2):45−50. doi: 10.7506/spkx1002-6630-201602008
    [19]
    吴迪, 赵贝贝, 耿曼璐, 等. 正交试验优化超声提取辣木叶总黄酮及其抗氧化活性研究[J]. 粮食与油脂,2017,30(8):96−99. [WU D, ZHAO B B, GENG M L, et al. Optimization of utrasonic extraction of total flavonoids fromMoringa oleifera leaves and their antioxidant activities by orthogonal test[J]. Cereals & Oils,2017,30(8):96−99. doi: 10.3969/j.issn.1008-9578.2017.08.025
    [20]
    王珊珊. 芫荽黄酮的提取、分离纯化及生物活性研究[D]. 哈尔滨: 哈尔滨商业大学, 2014.

    WANG S S. Study on extraction, isolation and purification biological activity of flavonoids in Coriandrum sativum[D]. Harbin: Harbin University of Commerce, 2014.
    [21]
    GAO C, DAVIN G C, LIU H Y, et al. Development of a thiolysis HPLC method for the analysis of procyanidins in cranberry products[J]. Journal of agricultural and food chemistry, 2018, 66(9):2159-2167.
    [22]
    陈瑾, 陈晶华, 刘利平, 等. 枸杞叶黄酮提取物的纯化及组成结构分析[J]. 食品工业科技,2019,40(18):28−34,40. [CHEN J, CHEN J H, LIU L P. Purification and structural analysis of flavonoids from Lycium barbarum leaves[J]. Science and Technology of Food Industry,2019,40(18):28−34,40.
    [23]
    王维维, 王亦军, 侯圣龙, 等. 昆仑雪菊中黄酮类化合物提取与纯化[J]. 广州化学,2014,39(4):22−27. [WANG W W, WANG Y J, HOU S L, et al. Extraction and isolation for flavonoids in Coreopsis tinctoria[J]. Guangzhou Chemistry,2014,39(4):22−27. doi: 10.3969/j.issn.1009-220X.2014.04.005
    [24]
    赵光远, 许艳华, 敬思群, 等. 高压微射流对葡聚糖理化性质和结构的影响[J]. 食品科技,2017,42(12):240−244. [ZHAO G Y, XU Y H, JING S Q, et al. Effect of high pressure microjet on physicochemical properties and structure of dextran[J]. Food Science and Technology,2017,42(12):240−244.
    [25]
    宋站立. 爪瓣山柑黄酮的优化提取、纯化、鉴定与生物活性研究[D]. 天津: 天津科技大学, 2019.

    SONG Z L. Optimization of extraction, purification, identification and biological activities of flavonoids from Cappairs himalayensis[D]. Tianjin: Tianjin University of Science and Technology, 2019.
    [26]
    杨小慧, 石光波, 拜晓彬, 等. 文冠果落果黄酮成分分析及抑菌性评价[J]. 食品科学,2018,39(10):53−58. [YANG X H, SHI G B, BAI X B, et al. Flavonoid content and antibacterial activity ofXanthoceras sorbifolia Bunge fruit drop[J]. Food Science,2018,39(10):53−58. doi: 10.7506/spkx1002-6630-201810009
    [27]
    WANG Y J , YAO S J, GUANG Y X, et al. A novel process for preparation of (1→3)-β-D-glucan sulphate by a heterogeneous reaction and its structural elucidation[J]. Carbohydrate Polymers, 2005, 59(1):93-99.
    [28]
    ZHANG Y L, XIA Y. Study on different extracts of Chrysanthemum indicum by fourier transform infrared spectroscopy[J]. Spectroscopy and Spectral Analysis,2012,32(12):3225−3228.
    [29]
    蔡冬宝, 施月, 马娇, 等. 高钙菜黄酮的分级分离各级分的抗氧化活性[J]. 食品工业科技,2019,40(5):108−113,123. [CAI D B, SHI Y, MA J, et al. Antioxidant activity of flavonoids fractionation extracts from Sedum aizoon L[J]. Science and Technology of Food Industry,2019,40(5):108−113,123.
    [30]
    ZHAO R M, ZHAO J, DUAN H X, et al. Green and efficient extraction of four bioactive flavonoids from pollen typhae by ultrasound-assisted deep eutectic solvents extraction[J]. Journal of Pharmaceutical and Biomedical Analysis,2018,161:246−253. doi: 10.1016/j.jpba.2018.08.048
    [31]
    何书美, 乔兰侠, 刘敬兰. 红外光谱法测定芹菜叶提取物中总黄酮的含量[J]. 分析科学学报,2008(2):201−204. [HE S M, QIAO L X, LIU J L. Quantitative determination of total flavonoids in the extraction of celery leaves by IR[J]. Journal of Analytical Science,2008(2):201−204.
    [32]
    LEI Z T, SUMMER B W, BHATIA A, et al. UHPLC-MS analyses of plant flavonoids[J]. Current Protocols in Plant Biology, 2018.
    [33]
    王雯皓, 梁雪, 焦莹莹, 等. 黄芫花黄酮提取物的制备、结构鉴定、含量测定和抗氧化活性研究[J/OL]. 山西大学学报(自然科学版): 1-9[2021-05-29].https://doi.org/10.13451/j.sxu.ns. 2020099.

    WANG W H, LIANG X, JIAO Y Y, et al. Preparation, structure identification, content determination and antioxidant activity of the flavonoid extracts from buds of Wikstroemia chamaedaphne[J/OL]. Journal of Shanxi University(Natural Science Edition): 1-9[2021-05-29].https://doi.org/10.13451/j.sxu.ns. 2020099.
    [34]
    LEE J I, NARAYAN M, BARRETT J S. Analysis and comparison of active constituents in commercial standardized silymarin extracts by liquid chromatography-electrospray ionization mass spectrometry[J]. Journal of Chromatography B, 2006, 845(1):95-103.
    [35]
    符群, 李卉, 王振宇, 等. 减压-超声辅助醇法提取薇菜黄酮及其对抗氧化活性的影响[J]. 现代食品科技,2018,34(3):113−120, 130. [FU Q, LI H, WANG Z Y, et al. The effect of decompression-ultrasonic assisted alcoholic extraction on the flavonoids from Osmunda japonica Thunb and its antioxidant activity[J]. Modern Food Science and Technology,2018,34(3):113−120, 130.
    [36]
    陈建福, 陈健旋, 林媛, 等. 芥菜总黄酮超声辅助提取工艺优化及其脂质抗氧化研究[J]. 食品研究与开发,2021,42(8):93−100. [CHEN J F, CHEN J X, LIN Y, et al. Optimization of ultrasound-assisted total flavonoid extraction from Brassica juncea and lipid antioxidant activity of the extract[J]. Food Research and Development,2021,42(8):93−100.
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