Enzymatic Characterization of Xylanase TgXyn2

GE Yuhang; CHEN Meixi; SUN Bo; WU Songna; WAN Qun

doi:10.13386/j.issn1002-0306.2021060100

Volume 43 Issue 4

Feb. 2022

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2022 > 43(4): 138-144. > DOI: 10.13386/j.issn1002-0306.2021060100

GE Yuhang, CHEN Meixi, SUN Bo, et al. Enzymatic Characterization of Xylanase TgXyn2[J]. Science and Technology of Food Industry, 2022, 43(4): 138−144. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021060100.

Citation:

PDF (2786 KB)

Enzymatic Characterization of Xylanase TgXyn2

1.
School of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
2.
College of Science, Nanjing Agricultural University, Nanjing 210095, China

More Information

Received Date: June 16, 2021
Available Online: December 19, 2021

Graphical Abstract

Abstract

Abstract

Objectives: This study aimed to discover a acidic xylanase TgXyn2 with high enzymatic activities at low temperatures, which might have potential application in food industry. Method: TgXyn2 was heterologously expressed in E.coli with the expression vector pCold-TF. Results: The optimum reaction condition of TgXyn2 was 35 ℃ and pH5.0, respectively. Its K_m was 1.287 μmol L⁻¹ and V_max was 2.083 μmol min⁻¹ mg⁻¹. Homology modeling showed that TgXyn2 had two antiparallel β-sheets and an α-helix, which was typical for the GH11 family. Conclusions: TgXyn2 had good enzymatic activities, which has potential application in food industry.
- Trichoderma guizhouense,
- xylanase,
- enzymatic activity,
- GH11,
- food industry

FullText(HTML)

References (32)

References

[1]	黄坤龙, 苏小运, 姚斌. 生长抑素和耐热木聚糖酶的融合表达及性质研究[J]. 生物技术通报,2020,36(9):235−243. [HUANG K L, SU X Y, YAO B. Fusion expression and characterization of somatostatin and thermostable xylanase[J]. Biotechnology Bulletin,2020,36(9):235−243.
[2]	王梦竹, 方颖, 李勍, 等. 植物细胞壁纳米结构与纳米纤维素的化学纯化处理结合机械解纤法制备[J]. 高分子学报,2020,51(6):586−597. [WANG M Z, FANG Y, LI Q, et al. Preparation of plant cell wall nanostructures and nano cellulose by chemical purification combined with mechanical fibrillation[J]. Acta Polymer Sinica,2020,51(6):586−597. doi: 10.11777/j.issn1000-3304.2020.20037
[3]	汤勇, 蔡俊. β-木糖苷酶的研究进展[J]. 中国酿造,2018,37(10):14−19. [TANG Y, CAI J. Research progress of β-xylosidase[J]. Brewing in China,2018,37(10):14−19. doi: 10.11882/j.issn.0254-5071.2018.10.004
[4]	CRISTINA V, JAVIER PASTOR F I, TERESA V, et al. Antioxidant activity of xylooligosaccharides produced from glucuronoxylan by Xyn10A and Xyn30D xylanases and eucalyptus autohydrolysates[J]. Carbohydrate Polymers,2018,194:43−50. doi: 10.1016/j.carbpol.2018.04.028
[5]	温博婷, 孙丽超, 王凤忠, 等. 微生物木聚糖酶的研究进展及其在食品领域的应用[J]. 生物产业技术,2017(5):81−86. [WEN B T, SUN L C, WANG F Z, et al. Research progress of microbial xylanase and its application in food field[J]. Biotechnology,2017(5):81−86.
[6]	刘凯, 肖付才. 木聚糖酶对面团特性及全麦面包品质影响[J]. 粮食与油脂,2021,34(4):23−26. [LIU K, XIAO F C. Effect of xylanase on dough characteristics and whole wheat bread quality[J]. Food and Oil,2021,34(4):23−26. doi: 10.3969/j.issn.1008-9578.2021.04.007
[7]	LOUW C, GRANGE D L, PRETORIUS I S, et al. The effect of polysaccharide-degrading wine yeast transformants on the efficiency of wine processing and wine flavour[J]. Journal of Biotechnology,2006,125(4):447−461. doi: 10.1016/j.jbiotec.2006.03.029
[8]	尚庆辉, 朴香淑, 王玉璘. 木聚糖酶在畜禽饲料中应用效果及其机理的研究进展[J]. 饲料工业,2017,38(2):17−24. [SHANG Q H, PU X S, WANG Y L. Research progress on application effect and mechanism of xylanase in animal feed[J]. Feed Industry,2017,38(2):17−24.
[9]	VAN Gool M P, VAN Muiswinkel G C J, HINZ S W A, et al. Two GH10 endo-xylanases from Myceliophthora thermophila C1 with and without cellulose binding module act differently towards soluble and insoluble xylans[J]. Bioresource Technology,2012,119(1):123−132.
[10]	BRADFORD MARION M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Academic Press,1976,72(1-2):248−254.
[11]	李治宏. 11家族木聚糖酶的热稳定性分子改造[D]. 南京: 南京农业大学, 2018: 3−34. LI Z H. Molecular modification of thermostability of 11 family xylanases[D]. Nanjing: Nanjing Agricultural University, 2018: 3−34.
[12]	BAILEY M J, BIELY P POUTANEN K. Interlaboratory testing of methods for assay of xylanase activity[J]. Elsevier,1992,23(3):257−270.
[13]	BIASINI M, BIENERT S, WATERHOUSE A, et al. SWISS-MODEL: Modelling protein tertiary and quaternary structure using evolutionary information[J]. Nucleic Acids Research, 2014, 42(Web Server issue).
[14]	李宁. 基于噬菌体展示的多肽预处理以及表位预测研究[D]. 成都: 电子科技大学, 2019: 13−75. LI N. Peptide pretreatment and epitope prediction based on phage display[D]. Chengdu: University of Electronic Science and Technology, 2019: 13−75.
[15]	马腾飞, 杨江科, 韩正刚, 等. 木聚糖酶基因XynB在毕赤酵母中的表达及酶学性质研究[J]. 食品科技,2018,43(5):1−9. [MA T F, YANG J K, HAN Z G, et al. Expression and characterization of xylanase gene XynB in Pichia pastoris[J]. Food science and technology,2018,43(5):1−9.
[16]	张伟, 刘秀霞, 杨艳坤, 等. 利用内源元件在谷氨酸棒杆菌中分泌表达木聚糖酶[J]. 生物工程学报,2019,35(3):425−434. [ZHANG W, LIU X X, YANG Y K, et al. Secretory expression of xylanase in Corynebacterium glutamicum using endogenous elements[J]. Acta Bioengineering,2019,35(3):425−434.
[17]	曹慧, 张会会, 张腾月, 等. 产低温纤维素酶和木聚糖酶真菌的筛选鉴定及酶学性质[J/OL]. 饲料研究: 1−8[2021-07-11]. http://kns.cnki.net/kcms/detail/11.2114.S.20201224.1754.002.html. CAO H, ZHANG H H, ZHANG T Y, et al. Screening, identification and enzymatic properties of fungi producing low temperature cellulase and xylanase[J/OL]. Feed Research: 1−8[2021-07-11]. http://kns.cnki.net/kcms/detail/11.2114.S.20201224.1754.002.html.
[18]	杨颖, 玉王宁, 金一, 等. 常压室温等离子体快速诱变绿色糖单孢菌筛选木聚糖酶高产菌株及其酶学性质研究[J]. 微生物学通报,2013,40(5):905−915. [YANG Y, YU W N, JIN Y, et al. Screening of high xylanase producing strain by atmospheric pressure and room temperature plasma rapid mutagenesis and its enzymatic properties[J]. Microbiology Bulletin,2013,40(5):905−915.
[19]	胡沂淮, 邵蔚蓝. 木聚糖酶[J]. 生命的化学, 2002, 22(3): 281−285. HU Y H, SHAO W L. Chemistry of xylanase[J]. Life, 2002, 22(3): 281−285.
[20]	WANG H, WANG K, GUAN Z, et al. Computational study of non-catalytic T-loop pocket on CDK proteins for drug development[J]. 中国物理B,2017,26(12):128702−128702.
[21]	冼亮, 秦艳, 朱婧, 等. 内切木聚糖酶的应用及其酶制剂开发技术[J]. 广西科学院学报,2020,36(3):300−308. [XIAN L, QIN Y, ZHU J, et al. Application of endoxylanase and development technology of enzyme preparation[J]. Journal of Guangxi Academy of Sciences,2020,36(3):300−308.
[22]	XING Hongguan, ZOU Gen, LIU Chunyan, et al. Improving the thermostability of a GH11 xylanase by directed evolution and rational design guided by B-factor analysis[J]. Enzyme and Microbial Technology,2021,143:109720−109720. doi: 10.1016/j.enzmictec.2020.109720
[23]	宋文芳, 胡国全, 徐彦胜. 一株低温厌氧纤维素降解细菌的分离、鉴定及其酶学性质的研究[J]. 西南农业学报,2011,24(4):1317−1322. [SONG W F, HU G Q, XU Y S. Isolation, identification and enzymatic properties of a low temperature anaerobic cellulose degrading bacterium[J]. Journal of Southwest Agriculture,2011,24(4):1317−1322. doi: 10.3969/j.issn.1001-4829.2011.04.018
[24]	郑亚伦, 夏瑛, 李良, 等. 源于解淀粉芽孢杆菌酸性木聚糖酶酶学性质的研究[J]. 食品与发酵工业,2020,46(24):58−65. [ZHENG Y L, XIA Y, LI L, et al. Study on the enzymatic properties of acid xylanase from Bacillus amylopectin[J]. Food and Fermentation Industry,2020,46(24):58−65.
[25]	怀文辉, 何秀萍, 郭文洁, 等. 微生物木聚糖降解酶研究进展及应用前景[J]. 微生物学通报,2000(2):137−139. [HUAI W H, HE X P, GUO T J, et al. Research progress and application prospect of microbial xylanase[J]. Bulletin of Microbiology,2000(2):137−139. doi: 10.3969/j.issn.0253-2654.2000.02.017
[26]	陈红歌, 朱静, 梁改芹, 等. 酸性木聚糖酶产生菌的筛选及产酶条件[J]. 微生物学报,1999(4):66−70. [CHEN H G, ZHU J, LIANG G Q, et al. Screening and enzyme producing conditions of acid xylanase producing strain[J]. Acta microbiologica Sinica,1999(4):66−70.
[27]	王剑, 尚庆辉, 张连华, 等. 木聚糖酶对肉鸡生长性能和肠道健康的影响[J/OL]. 动物营养学报: 1−13[2021-06-09]. http://kns.cnki.net/kcms/detail/11.5461.S.20210419.1452.092.html. WANG J, SHANG Q H, ZHANG L H, et al. . Effects of xylanase on growth performance and intestinal health of broilers[J/OL]. Acta Zoologica Sinica: 1−13[2021-06-09]. http://kns.cnki.net/kcms/detail/11.5461.S.20210419.1452.092.html.
[28]	ERI T, AKINORI K, SATOSHI W, et al. Gastric and intestinal proteases resistance of chicken acidic chitinase nominates chitin-containing organisms for alternative whole edible diets for poultry[J]. Scientific Reports,2017,7(1):20878−20881.
[29]	朱崇淼, 毛胜勇, 孙云章, 等. 厌氧真菌发酵液中木聚糖酶活力的初步研究[J]. 南京农业大学学报,2004(1):120−123. [ZHU C M, MAO S Y, SUN Y Z, et al. Preliminary study on xylanase activity in anaerobic fungal fermentation broth[J]. Journal of Nanjing Agricultural University,2004(1):120−123.
[30]	LIU Xueqiang, LIU Yu, JIANG Zhengqiang, et al. Biochemical characterization of a novel xylanase from Paenibacillus barengoltzii and its application in xylooligosaccharides production from corncobs[J]. Food Chemistry,2018,264:310−318. doi: 10.1016/j.foodchem.2018.05.023
[31]	关莹, 薛敏, 王伟. 饲用酶制剂在水产动物中应用的最新研究进展[J]. 中国渔业质量与标准,2021,11(1):61−67. [GUAN Y, XUE M, WANG W. The latest research progress on the application of feed enzymes in aquatic animals[J]. China Fisheries Quality and Standard,2021,11(1):61−67.
[32]	ZHOU Yan, YUAN Zhongyao, HAO Weiwei, et al. Advances in alfalfa transgenic technology and its application[J]. Agricultural Biotechnology,2013,2(Z1):7−12.

Supplements (0)

Cited By

Cited by

Periodical cited type(9)

1.	刘学艳，吴成远，徐婷，罗正飞，刘艳红，王绍梅. 不同品种工夫红茶挥发性物质研究. 中国茶叶. 2025(04): 62-67 .
2.	徐旭华，黄文洁，陈旭峰，陈园园，吴绍文，李红建，晏石娟. ‘丹霞2号’红茶加工过程中品质特征成分的动态变化研究. 园艺学报. 2024(01): 145-161 .
3.	孙宏，姚晓红，吴逸飞，王新，沈琦，汤江武. 粉绿狐尾藻固态发酵饲料的营养成分及其代谢物分析. 中国畜牧杂志. 2023(04): 186-192 .
4.	唐佳代，冉光耀，陈诺，王芙蓉，赵腾飞，冷枝，王怡，王士超. 基于非靶向代谢组学分析不同陈化时间老鹰茶代谢产物的差异. 中国酿造. 2023(09): 115-119 .
5.	杨云飞，王玉婉，林家正，涂政，蓝天梦，陈琳，叶阳. 发酵中氧对红茶品质的影响及富氧发酵工艺优化. 食品工业科技. 2023(19): 199-207 . 本站查看
6.	夏长杙，冉乾松，蒲璐璐，刘亚兵. 不同发酵方式对古茶树鲜叶制备工夫红茶品质的影响. 中国酿造. 2023(10): 127-135 .
7.	陈霞，唐晨阳，马昕怡，邵童，李晓兵，顾瑞霞. 超微茶粉对益生菌慕斯品质及抗氧化活性的影响. 食品与发酵工业. 2022(10): 194-198 .
8.	刘亚芹，王辉，杨霁虹，周汉琛，黄建琴，雷攀登. 基于滋味成分变化的祁门红茶发酵程度差异研究. 茶叶通讯. 2022(02): 193-201 .
9.	曾议霆，吴雪莉，杨春梅，刘高杰，高丙德，唐克纯. 基于非靶向代谢组学比较不同发酵方式红茶滋味物质差异. 食品安全质量检测学报. 2022(16): 5288-5296 .