Inhibitory Effect of <i>Tremella fuciformis</i> Polysaccharide on Starch Digestive Enzymes and Its Action Mechanism

DU Qinling; YANG Fang; XU Wen; MIAO Ting; CAO Junjie; JIA Dongying

doi:10.13386/j.issn1002-0306.2021060084

Volume 43 Issue 2

Jan. 2022

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Science and Technology of Food Industry > 2022 > 43(2): 120-125. > DOI: 10.13386/j.issn1002-0306.2021060084

DU Qinling, YANG Fang, XU Wen, et al. Inhibitory Effect of Tremella fuciformis Polysaccharide on Starch Digestive Enzymes and Its Action Mechanism[J]. Science and Technology of Food Industry, 2022, 43(2): 120−125. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021060084.

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Inhibitory Effect of Tremella fuciformis Polysaccharide on Starch Digestive Enzymes and Its Action Mechanism

1.
College of Biomass Science & Engineering, Sichuan University, Chengdu 610065, China
2.
PepsiCo Foods (China) Co., Ltd., Shanghai 200023, China
3.
Chengdu Julong Biotechnology Co., Ltd., Chengdu 611131, China

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Received Date: June 09, 2021
Available Online: November 20, 2021

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Abstract

Abstract

Objective: To investigate the inhibitory effect of Tremella fuciformis polysaccharide (TP) on pancreatic α-amylase and α-glucosidase and its action mechanism. Methods: TP with a total sugar content of 92.45% was obtained by alkaline extraction from dried Tremella fuciformis, enzymatic deproteinization and column chromatography separation in turn. The inhibitory effect of TP on pancreatic α-amylase and α-glucosidase was measured by visible spectrophotometry, and its action on the structures of these two enzymes was characterized by fluorescence spectrometry and circular dichroism. Results: TP could inhibit the activities of these two enzymes, and its inhibition on pancreatic α-amylase was significantly higher than that on α-glucosidase, and its half inhibitory concentrations (IC₅₀) on these two enzymes were 7.6835 and 16.9306 mg/mL, respectively. TP inhibited the activity of the enzymes by interacting with them. TP interacted strongly with pancreatic α-amylase. It could statically quench pancreatic α-amylase and change its secondary structure. However, TP interacted weakly with α-glucosidase and could not change its secondary structure. Conclusion: TP inhibited the activity of starch-digestive enzymes by interacting with them.
- Tremella fuciformis polysaccharide,
- pancreatic α-amylase,
- α-glucosidase,
- inhibition,
- mechanism

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References (30)

References

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