Comparison between Three Different Origins of <i>Dendrobium officinale</i> Polysaccharides and Their Initial Pharmacological Activity Evaluations

HUANG Junbin; DING Jie; ZHU Haimei; LI Yunrong; HUANG Dandan; WEI Gang

doi:10.13386/j.issn1002-0306.2021050262

Volume 43 Issue 5

Feb. 2022

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Science and Technology of Food Industry > 2022 > 43(5): 71-78. > DOI: 10.13386/j.issn1002-0306.2021050262

HUANG Junbin, DING Jie, ZHU Haimei, et al. Comparison between Three Different Origins of Dendrobium officinale Polysaccharides and Their Initial Pharmacological Activity Evaluations[J]. Science and Technology of Food Industry, 2022, 43(5): 71−78. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021050262.

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Comparison between Three Different Origins of Dendrobium officinale Polysaccharides and Their Initial Pharmacological Activity Evaluations

1.
Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510120, China
2.
Guangzhou University of Chinese Medicine, Guangzhou 510006, China

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Received Date: May 30, 2021
Available Online: January 04, 2022

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Abstract

Abstract

In this paper, comparisons of the the molecular weight, monosaccharide composition, infrared spectrum were made between Dendrobium officinale polysaccharides from three different provenances, namely Yunnan, Danxia and Zhejiang. Together with the evaluation of preliminary pharmacological activity, the differences of Dendrobium candidum from different areas were discussed. High performance gel permeation chromatography (HPGPC) was used to determine the molecular weight of the purified polysaccharide fragments, high performance liquid chromatography (HPLC) was used to determine the composition of monosaccharides, infrared spectroscopy (IR) was used to determine the structure of polysaccharides, and MTT method was used to observe the effect of polysaccharide fragments on the proliferation of two kinds of tumor cells (Hela cells and HT-29 cells). The results showed that the molecular weight of the polysaccharide fragment of Dendrobium officinale in Yunnan was 757623 u, the molecular weight of polysaccharide fragment of Dendrobium officinale in Zhejiang was 605958 u, and the molecular weight of Dendrobium officinale polysaccharide fragment in Danxia was 663240 u. The monosaccharide composition of Dendrobium officinale polysaccharide was mainly mannose and glucose. In terms of the ratio of mannose to glucose, Yunnan species>Danxia species>Zhejiang species. Moreover, the ratio of polysaccharides would further increase after purification. Infrared spectrum absorption and characteristic peaks suggested that Dendrobium officinale polysaccharides mainly contained mannose and glucose, and the sugars contained were β-type. Purification of Dendrobium officinale polysaccharides could change the composition ratio of monosaccharides and increase the content of mannose. There were certain differences in molecular weight, monosaccharide composition and infrared spectroscopy of Dendrobium officinale polysaccharides from different provenances, and they had certain inhibitory effect on HT-29 cells. Particularly, the polysaccharide fragments of Dendrobium officinale from Danxia species had a relatively good inhibitory effect on the proliferation of the two tumor cells under the 48 h experimental determination. The specific correlation needs to be further studied.

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[1]	焦连魁, 曾燕, 张继聪, 等. 石斛属优质道地药材生产技术概述[J]. 中国现代中药,2021,23(4):734−740. [JIAO L K, ZENG Y, ZHANG J C, et al. A summary of cultivation technology of high quality and daodi Dendrobium medicinal materials[J]. Modern Chinese Medicine,2021,23(4):734−740.
[2]	罗迪, 庞璐, 赵兴, 等. 浅析《本草纲目》中铁皮石斛的功能主治[J]. 湖南中医杂志,2013,29(3):108−109. [LUO D, PANG L, ZHAO X, et al. Analysis on the functions and indications of Dendrobium officinale in "Compendium of Materia Medica"[J]. Hunan Journal of Traditional Chinese Medicine,2013,29(3):108−109.
[3]	XING L, MIAO Y, LI N, et al. Molecular structure features and lactic acid fermentation behaviors of water- and alkali-soluble polysaccharides from Dendrobium officinale[J]. Journal of Food Science and Technology-Mysore,2021,58(2):532−540. doi: 10.1007/s13197-020-04564-6
[4]	奚航献, 刘晨, 刘京晶, 等. 铁皮石斛化学成分、药理作用及其质量标志物(Q-marker)的预测分析[J]. 中草药,2020,51(11):3097−3109. [XI H X, LIU C, LIU J J, et al. Chemical components and pharmacological action for Dendrobium officinale and its prediction analysis on Q-marker[J]. Chinese Traditional and Herbal Drugs,2020,51(11):3097−3109.
[5]	ZUO S, YU H, ZHANG W, et al. Comparative metabolomic analysis of Dendrobium officinale under different cultivation substrates[J]. Metabolites,2020,10(8):1−14.
[6]	REN Z, QIU F, WANG Y, et al. Network analysis of transcriptome and LC-MS reveals a possible biosynthesis pathway of anthocyanins in Dendrobium officinale[J]. Biomed Research International,2020,2020:6512895.
[7]	LI M, YUE H, WANG Y, et al. Intestinal microbes derived butyrate is related to the immunomodulatory activities of Dendrobium officinale polysaccharide[J]. International Journal of Biological Macromolecules,2020,149:717−723. doi: 10.1016/j.ijbiomac.2020.01.305
[8]	HU J, HUANG W, ZHANG F, et al. Variability of volatile compounds in the medicinal plant Dendrobium officinale from different regions[J]. Molecules,2020,25(21):5046−5055. doi: 10.3390/molecules25215046
[9]	HUANG S, CHEN F, CHENG H, et al. Modification and application of polysaccharide from traditional Chinese medicine such as Dendrobium officinale[J]. International Journal of Biological Macromolecules,2020,157:385−393. doi: 10.1016/j.ijbiomac.2020.04.141
[10]	TAO S, HUANG C, TAN Z, et al. Effect of the polysaccharides derived from Dendrobium officinale stems on human HT-29 colorectal cancer cells and a zebrafish model[J]. Food Bioscience,2021,41(5):100995.
[11]	FU Y, ZHANG J, CHEN K, et al. An in vitro fermentation study on the effects of Dendrobium officinale polysaccharides on human intestinal microbiota from fecal microbiota transplantation donors[J]. J Funct Food,2019,53:44−53. doi: 10.1016/j.jff.2018.12.005
[12]	LI L, YAO H, LI X, et al. Destiny of Dendrobium officinale polysaccharide after oral administration: Indigestible and nonabsorbing, ends in modulating gut microbiota[J]. Journal of Agricultural and Food Chemistry,2019,67(21):5968−5977. doi: 10.1021/acs.jafc.9b01489
[13]	CHEN H, NIE Q, HU J, et al. Metabolism amelioration of Dendrobium officinale polysaccharide on type II diabetic rats[J]. Food Hydrocolloids,2020,102:105582.
[14]	LIANG J, LI H, CHEN J, et al. Dendrobium officinale polysaccharides alleviate colon tumorigenesis via restoring intestinal barrier function and enhancing anti-tumor immune response[J]. Pharmacological Research,2020,148:104417.
[15]	CHEN Y, WANG Y, LYU P, et al. Comparative transcriptomic analysis reveal the regulation mechanism underlying MeJA-induced accumulation of alkaloids in Dendrobium officinale[J]. Journal of Plant Research,2019,132(3):419−429. doi: 10.1007/s10265-019-01099-6
[16]	谢唐贵, 陈敬民, 李燕婧. 不同产地铁皮石斛水提物的抗疲劳作用研究[J]. 云南中医中药杂志,2018,39(8):66−67. [XIE T G, CHEN J M, LI Y J. Study on anti-fatigue effect of aqueous extracts from Dendrobium candidum in different producing areas[J]. Yunnan Journal of Traditional Chinese Medicine,2018,39(8):66−67. doi: 10.3969/j.issn.1007-2349.2018.08.030
[17]	陈燕兰, 钟淳菲, 徐雅囡, 等. 不同地区铁皮石斛的品质差异研究[J]. 食品与发酵工业,2020,46(8):123−130. [CHEN Y L, ZHONG C F, XU Y N, et al. Study on quality differences of Dendrobium officinale in different areas[J]. Food and Fermentation Industries,2020,46(8):123−130.
[18]	MAI Y, YANG Z, JI X, et al. Comparative analysis of transcriptome and metabolome uncovers the metabolic differences between Dendrobium officinale protocorms and mature stems[J]. All Life,2020,13(1):346−359. doi: 10.1080/26895293.2020.1781699
[19]	韩邦兴, 陈凌霄, 邓勇, 等. 糖谱法结合多元色谱分析比较铁皮石斛功能性多糖结构特征[J]. 药物分析杂志,2018,38(1):41−49. [HAN B X, CHEN L X, DENG Y, et al. Characterization and comparison of specific polysaccharides in Dendrobium officinale by using saccharide mapping and chromatographic methods[J]. Chin J Pharm Anal,2018,38(1):41−49.
[20]	LUO Y, REN Z, DU B, et al. Structure identification of vicenin II extracted from Dendrobium officinale and the reversal of TGF-1-induced epithelial-mesenchymal transition in lung adenocarcinoma cells through TGF-/Smad and PI3K/Akt/mTOR signaling pathways[J]. Molecules,2019,24(1):144−160. doi: 10.3390/molecules24010144
[21]	黄丽, 文凤娟, 李桂琼, 等. 铁皮石斛多糖提取工艺及优化研究[J]. 云南农业大学学报(自然科学),2017,32(5):884−888. [HUANG L, WEN F J, LI G Q, et al. Research on optimizing extraction technology of polysaccharides from Dendrobium officinale[J]. Journal of Yunnan Agricultural University (Natural Science),2017,32(5):884−888.
[22]	ZHANG X, LUO Y, WEI G, et al. Physicochemical and antioxidant properties of the degradations of polysaccharides from Dendrobium officinale and their suitable molecular weight range on inducing Hela cell apoptosis[J]. Evidence-Based Complementary and Alternative Medicine,2019,2019:4127360.
[23]	TAO S, LEI Z, HUANG K, et al. Structural characterization and immunomodulatory activity of two novel polysaccharides derived from the stem of Dendrobium officinale Kimura et Migo[J]. J Funct Food,2019,57:121−134. doi: 10.1016/j.jff.2019.04.013
[24]	YANG K, LU T, ZHAN L, et al. Physicochemical characterization of polysaccharide from the leaf of Dendrobium officinale and effect on LPS induced damage in GES-1 cell[J]. International Journal of Biological Macromolecules,2020,149:320−330. doi: 10.1016/j.ijbiomac.2020.01.026
[25]	KE Y, ZHAN L, LU T, et al. Polysaccharides of Dendrobium officinale Kimura & Migo leaves protect against ethanol-induced gastric mucosal injury via the AMPK/mTOR signaling pathway in vitro and vivo[J]. Frontiers in Pharmacology, 2020,5263:49.
[26]	YU Z, ZHANG G, DA SILVA J A T, et al. The beta-1, 3-galactosetransferase gene DoGALT2 is essential for stigmatic mucilage production in Dendrobium officinale[J]. Plant Science,2019,287:110179.
[27]	KUANG M, LI J, YANG X, et al. Structural characterization and hypoglycemic effect via stimulating glucagon-like peptide-1 secretion of two polysaccharides from Dendrobium officinale[J]. Carbohydrate Polymers,2020,241:116326.
[28]	黄俊彬, 黄丹丹, 陈欢欢, 等. 铁皮石斛多糖分子量测定及其影响因素分析[J]. 食品工业科技,2017,38(7):81−85. [HUANG J B, HUANG D D, CHEN H H, et al. The effects of heating and ultrasonic degradation on the molecular weight of Dendrobium officinale polysaccharide[J]. Science and Technology of Food Industry,2017,38(7):81−85.
[29]	国家药典委员会. 中华人民共和国药典. 2015版一部[M]. 北京: 中国医药科技出版社, 2015: 282. National Pharmacopoeia Committee. Pharmacopoeia of the People's Republic of China. 2015 Edition One[M]. Beijing: China Medical Science and Technology Press, 2015: 282.
[30]	鲍素华, 查学强, 郝杰, 等. 不同分子量铁皮石斛多糖体外抗氧化活性研究[J]. 食品科学,2009,30(21):123−127. [BAO S H, ZHA X Q, HAO J, et al. In vitro antioxidant activity of polysaccharides with different molecular weights from Dendrobium candidum[J]. Food Science,2009,30(21):123−127. doi: 10.3321/j.issn:1002-6630.2009.21.029
[31]	陶盛昌. 铁皮石斛水溶性多糖分离纯化、结构特征及免疫活性研究[D]. 广州: 广州中医药大学, 2016. TAO S C. Study on structural idenfication and immune activity of water-soluble polysaccharides from the stems of Dendrobium officinale Kimum et Migo[D]. Guangzhou: Guangzhou University of Chinese Medicine, 2016.

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