LU Xintong, QI Xin, GAO Xuefeng, et al. Effect of Irradiated Process on Anti-fatigue Activity of Inonotus obliquus Polysaccharide[J]. Science and Technology of Food Industry, 2022, 43(3): 351−357. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021050192.
Citation: LU Xintong, QI Xin, GAO Xuefeng, et al. Effect of Irradiated Process on Anti-fatigue Activity of Inonotus obliquus Polysaccharide[J]. Science and Technology of Food Industry, 2022, 43(3): 351−357. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021050192.

Effect of Irradiated Process on Anti-fatigue Activity of Inonotus obliquus Polysaccharide

More Information
  • Received Date: May 20, 2021
  • Available Online: December 03, 2021
  • Objective: To study the anti-fatigue effect of irradiated Inonotus obliquus polysaccharide on mice. Method: 60Co-γ rays were irradiated to obtain 5, 10, 20, 30 kGy irradiation doses and unirradiated Inonotus obliquus bacteria powder to produce polysaccharides. Divided into a control group, an irradiated high-medium-low-dose group, and a non-irradiated high-dose group. Male Kunming mice were given four weeks continuous gavage to determine the rod climbing, exhausted swimming time, changes in liver glycogen(LG), muscle glycogen(MG), blood lactate(BLA), lactate dehydrogenase(LDH), serum urea nitrogen(SUN), superoxide dismutase(SOD) and malondialdehyde(MDA) in skeletal muscle. Result: The optimal radiation dose measured in the experiment was 20 kGy. Each dose group had no significant effect on the body weight of the mice(P>0.05). The high and medium dose groups and the non-irradiated groups significantly prolonged the rod climbing time and exhausted swimming time of the mice(P<0.05). Irradiation of Inonotus obliquus polysaccharides increased the contents of liver glycogen and muscle glycogen in mice to the highest levels of 28.90 mg/g and 10.58 mg/g, reduced the contents of BLA, SUN and MDA in mice, and increased LDH and SOD activities in mice. The highest activity was 5308.80 U/L and 11.44 nU/mg. Moreover, LG, MG, LDH, SUN, SOD, MDA in the high-dose irradiated group were significantly different from those in the non-irradiated group(P<0.05), and there was no significant difference in BLA between the two groups(P>0.05). Conclusion: This test proves that the anti-fatigue effect of the irradiated Inonotus obliquus polysaccharide is further improved compared with the non-irradiated group, and that the irradiated Inonotus obliquus polysaccharide can relieve fatigue symptoms to a certain extent.
  • [1]
    刘睿, 康家伟, 珠娜, 等. 核桃低聚肽对亚健康大鼠氧化应激和炎症反应的影响[J]. 食品工业科技,2020,41(4):316−321. [LIU R, KANG J W, ZHU N, et al. Effects of walnut oligopeptides on oxidative stress and inflammation in sub-healthy rats[J]. Food Industry Science and Technology,2020,41(4):316−321.
    [2]
    黄桂丹. 60Co-γ射线辐射育种研究进展[J]. 林业与环境科学,2016,32(2):107−111. [HUANG G D. Research progress of 60Co-γ-ray radiation breeding[J]. Forestry and Environmental Science,2016,32(2):107−111. doi: 10.3969/j.issn.1006-4427.2016.02.020
    [3]
    PEDERSEN T H, NIELSEN O B, LAMB G D, et al. Stephenson intracellular acidosis enhances the excitability of working muscle[J]. Science, 305(2004): 1144−1147.
    [4]
    闫立珠, 刘光斌. 金锁二仙口服液提高肾虚小鼠体能及抗疲劳的实验研究[J]. 临床合理用药杂志,2017,10(1):100−101. [YAN L Z, LIU G B. Experimental study of Jinsuo Erxian oral liquid on improving physical performance and anti-fatigue in mice with kidney deficiency[J]. Journal of Clinical Rational Use,2017,10(1):100−101.
    [5]
    朴成浩. 辐照处理长白山红景天抗氧化和抗疲劳作用研究[D]. 延吉: 延边大学, 2020.

    PIAO C H. Research on the anti-oxidation and anti-fatigue effects of Rhodiola radiata in Changbai Mountain[D]. Yanji: Yanbian University, 2020.
    [6]
    李欣. 黑灵芝多糖缓解运动性疲劳的效果[J]. 食品研究与开发,2021,42(4):59−65. [LI X. The effect of black Ganoderma polysaccharide on relieving exercise fatigue[J]. Food Research and Development,2021,42(4):59−65.
    [7]
    黄年来. 俄罗斯神秘的民间药用真菌—桦褐孔菌[J]. 中国食用菌,2002,21(4):7−8. [HUANG N L. The mysterious folk medicinal fungus in Russia—Inonotus obliquus[J]. Edible Fungus in China,2002,21(4):7−8. doi: 10.3969/j.issn.1003-8310.2002.04.002
    [8]
    李艳青, 齐婷娟, 郭婷婷, 等. 桦褐孔菌乙酸乙酯提取物抗食管癌作用的研究[J]. 生物技术通讯,2019,30(1):15−19. [LI Y Q, QI T J, GUO T T, et al. Anti-esophageal cancer effect of ethyl acetate extract of Inonotus obliquus[J]. Biotechnology Communications,2019,30(1):15−19. doi: 10.3969/j.issn.1009-0002.2019.01.003
    [9]
    马丹丹, 汪雯翰, 薛蓓, 等. 桦褐孔菌降糖活性成分及治疗糖尿病机制研究进展[J]. 南京师大学报(自然科学版),2019,42(4):111−117. [MA D D, WANG W H, XUE B, et al. Research progress on the hypoglycemic active ingredients of Inonotus obliquus and the mechanism of treatment of diabetes[J]. Journal of Nanjing Normal University(Natural Science Edition),2019,42(4):111−117.
    [10]
    GLAMO C LIJA J, C IRIC A, NIKOLIC M, et al. Chemical characterization and biological activity of chaga(Inonotus obliquus), a medicinal "mushroom"[J]. Journal of Ethnopharmacology,2015,162:323−332. doi: 10.1016/j.jep.2014.12.069
    [11]
    DU X J, MU H M, ZHOU S, et al. Chemical analysis and antioxidant activity of polysaccharides extracted from Inonotus obliquus sclerotia[J]. International Journal of Biological Macromolecules,2013(62):691−696.
    [12]
    王梦雅, 赵喆禛, 薛娇, 等. 桦褐孔菌纯化多糖体外降血糖活性研究[J]. 食品工业科技,2020,41(10):316−320, 326. [WANG M Y, ZHAO Z Z, XUE J, et al. In vitro hypoglycemic activity of purified polysaccharide from Inonotus obliquus[J]. Food Industry Science and Technology,2020,41(10):316−320, 326.
    [13]
    魏振华, 陈妮, 范秋领, 等. 桦褐孔菌多糖对糖尿病大鼠糖脂代谢水平的影响[J]. 菌物研究,2020,18(3):195−200. [WEI Z H, CHEN N, FAN Q L, et al. Effects ofInonotus obliquus polysaccharides on the level of glucose and lipid metabolism in diabetic rats[J]. Mycology Research,2020,18(3):195−200.
    [14]
    崔杰, 于新, 王立勋, 等. 桦褐孔菌化学成分和药理作用研究进展[J]. 河北中医药学报,2017,32(3):48−53. [CUI J, YU X, WANG L X, et al. Research progress on the chemical constituents and pharmacological effects of Inonotus obliquus[J]. Hebei Journal of Traditional Chinese Medicine,2017,32(3):48−53.
    [15]
    BALANDAYKIN M E, ZMITROVICH I V. Review on chaga medicinal mushroom, Inonotus obliquus(higher basidiomycetes): Realm of medicinal applications and approaches on estimating its resource potential[J]. Int J Med Mushrooms,2015,17(2):95−104. doi: 10.1615/IntJMedMushrooms.v17.i2.10
    [16]
    SHIBNEV V A, GARAEV T M, FINOGENOVA M P, et al. Antiviral activity of aqueous extracts of the birch fungusInonotus obliquus on the human immunodeficiency virus[J]. Voprosy Virusologii,2015,60(2):35.
    [17]
    孔广红, 柳觐, 倪书邦. 澳洲坚果接穗60Co-γ射线辐射诱变育种适宜剂量的研究[J]. 西南农业学报,2016,29(1):39−43. [KONG G H, LIU G, NI S B. Study on the appropriate dose of 60Co-γ ray radiation mutation breeding for macadamia scion[J]. Southwest Agricultural Journal,2016,29(1):39−43.
    [18]
    陈湘霞. 辐照对食品中营养成分的影响分析[J]. 技术与市场,2017,24(9):67−68. [CHEN X X. Analysis of the influence of irradiation on the nutritional components of food[J]. Technology and Market,2017,24(9):67−68. doi: 10.3969/j.issn.1006-8554.2017.09.029
    [19]
    李斌, 杨秦, 肖洪, 等. 辐照对食品品质的影响及辐照食品的研究进展[J]. 粮食与油脂,2019,32(4):4−6. [LI B, YANG Q, XIAO H, et al. The effect of irradiation on food quality and the research progress of irradiated food[J]. Cereals and Oils,2019,32(4):4−6. doi: 10.3969/j.issn.1008-9578.2019.04.002
    [20]
    SONG HY, KIM HM, KIM WS, et al. Effect of γ-irradiation on the anti-oxidant and anti-melanogenic activity of black ginseng extract in B16F10 melanoma cells[J]. Radiat Phys Chem,2018,149:33−40. doi: 10.1016/j.radphyschem.2018.03.008
    [21]
    王润. 食品辐照保藏技术对食品品质的影响以及安全性讨论[J]. 现代食品,2015(13):56−58. [WANG R. The influence of food radiation preservation technology on food quality and safety discussion[J]. Modern Food,2015(13):56−58. doi: 10.3969/j.issn.1007-3582.2015.13.028
    [22]
    PATHAK B, OMRE P K, BISHT B, et al. Effect of thermal and non-thermal processing methods on food allergens[J]. Progressive Research–An International Journal,2018(13):314−319.
    [23]
    陈浙娅, 殷朝敏, 史德芳, 等. 辐照处理对裂褶菌多糖理化性质及生物活性的影响[A]. 中国菌物学会, 2019: 1.

    CHEN Z Y, YIN C M, SHI D F, et al. The effect of irradiation treatment on the physicochemical properties and biological activity of Schizophyllum polysaccharide[A]. Chinese Society of Mycology, 2019: 1.
    [24]
    高洋, 邬丹, 付雯雯, 等. 桦褐孔菌不同乙醇浓度提取物中有效成分含量测定[J]. 长春中医药大学学报,2020,36(6):1157−1159. [GAO Y, WU D, FU W W, et al. Determination of active ingredients in different ethanol concentrations of Inonotus obliquus extracts[J]. Journal of Changchun University of Traditional Chinese Medicine,2020,36(6):1157−1159.
    [25]
    张淑萍, 王亚军, 胡春荣, 等. 刺五加提取物对睡眠剥夺小鼠抗氧化抗疲劳作用的研究[J]. 黑龙江医药科学,2015,38(6):1−3. [ZHANG S P, WANG Y J, HU C R, et al. Study on the antioxidant and anti-fatigue effects of Acanthopanax senticosus extract on sleep deprived mice[J]. Black Longjiang Medical Science,2015,38(6):1−3.
    [26]
    中华人民共和国卫生部. 保健食品检验与评价技术规范[S]. 北京: 清华同方电子出版社, 2003: 87−93.

    Ministry of Health of the People's Republic of China. Technical specifications for health food inspection and evaluation[S]. Beijing: Tsinghua Tongfang Electronic Press, 2003: 87−93.
    [27]
    林伟龙, 李兆安, 蔡金艳. 复方赤灵芝营养粉对小鼠抗疲劳作用和氧化损伤模型小鼠的影响[J]. 广东药科大学学报,2019,35(2):242−245. [LIN W L, LI Z A, CAI J Y. The effect of compound red Ganoderma lucidum nutritional powder on the anti-fatigue effect of mice and oxidative damage model mice[J]. Journal of Guangdong Pharmaceutical University,2019,35(2):242−245.
    [28]
    ZENG Y, ZHANG J, ZHANG Y, et al. Prebiotic, immunomodulating and antifatigue effects of konjac oligosaccharide[J]. Food Sci,2018,83(12):3110−3117. doi: 10.1111/1750-3841.14376
    [29]
    MATSUOKA R, KIMURA M, UNO S, et al. Egg white hydrolysate improves fatigue due to short-term swimming load test in mice[J]. Food Sci,2018,6(8):2314−2320.
    [30]
    席晓志, 郭弘, 郭莎莎. 洋参蒺藜多糖片的制备及其抗疲劳功能研究[J]. 中国食品添加剂,2018,10(2):67−72. [XI X Z, GUO H, GUO S S. Preparation of Tribulus terrestris polysaccharide tablets and its anti-fatigue function[J]. China Food Additives,2018,10(2):67−72. doi: 10.3969/j.issn.1006-2513.2018.02.004
    [31]
    史得君, 黄柏申, 崔清美. 黑参对昆明种小鼠的抗疲劳作用研究[J]. 延边大学农学学报,2016,38(3):204−208. [SHI D J, HUANG B S, CUI Q M. Study on the anti-fatigue effect of black ginseng on Kunming mice[J]. Journal of Agricultural Science Yanbian University,2016,38(3):204−208.
    [32]
    龚志华. 茯苓辐照降解及其产物生物活性研究[D]. 长沙: 湖南农业大学, 2010.

    GONG Z H. Radiation degradation of Poria and the biological activity of its products[D]. Changsha: Hunan Agricultural University, 2010.
    [33]
    曹利丹. 桦褐孔菌多糖对小鼠抗运动疲劳的影响[J]. 赤峰学院学报,2017,33(22):149−150. [CAO L D. The effect of Inonotus obliquus polysaccharides on resistance to exercise fatigue in mice[J]. Journal of Chifeng University,2017,33(22):149−150.
    [34]
    赵小宁. 牛大力抗疲劳药效筛选及其作用机理研究[D]. 广州: 广州中医药大学, 2015.

    ZHAO X N. Anti-fatigue efficacy screening of Niu Dali and its mechanism of action[D]. Guangzhou: Guangzhou University of Traditional Chinese Medicine, 2015.
    [35]
    黄铃. 桦褐孔菌液体发酵工艺及其多糖抗疲劳的研究[D]. 福州: 福建农林大学, 2011.

    HUANG L. Study on the liquid fermentation process of Inonotus obliquus and the anti-fatigue of its polysaccharides[D]. Fuzhou: Fujian Agriculture and Forestry University, 2011.
    [36]
    ZHONG X H, ZHONG Y, YANG S Y. Effect of In­onotus obliquus polysaccharides on physical fatigue in mice[J]. Tradit Chin Med,2015,35(4):468−472. doi: 10.1016/S0254-6272(15)30126-6
    [37]
    焦迎春, 旷慧, 吴嘉南. 柴达木大肥菇多糖对小鼠的抗疲劳作用[J]. 现代食品科技,2018,34(8):24−30. [JIAO Y C, KUANG H, WU J N. Anti-fatigue effect of Chaidamu big fat mushroom polysaccharide on mice[J]. Modern Food Science and Technology,2018,34(8):24−30.
    [38]
    郭瑞. 白藜芦醇抗疲劳作用及其机理研究[J]. 食品研究与开发,2018,39(24):174−179. [GUO R. Research on the anti-fatigue effect of resveratrol and its mechanism[J]. Food Research and Development,2018,39(24):174−179. doi: 10.3969/j.issn.1005-6521.2018.24.030
    [39]
    侯登勇, 张建, 何颖, 等. 核桃分心木水提物抗疲劳作用研究[J]. 中国食物与营养,2021,27(9):73−77. [HOU D Y, ZHANG J, HE Y, et al. Study on the anti-fatigue effect of the water extract of walnut distracted wood[J]. Chinese Food and Nutrition,2021,27(9):73−77.
    [40]
    Prinsen H, DIJK J P V, ZWARTS M J , et al. The role of central and peripheral muscle fatigue in postcancer fatigue: A randomized controlled trial[J]. Pain Symptom Manag,2015,49:173−182. doi: 10.1016/j.jpainsymman.2014.06.020
    [41]
    LUO C H, XU X R, WEI X C, et al. Natural medicines for the treatment of fatigue: Bioactive components, pharmacology and mechanisms[J]. Pharmacol Res,2019,148:1−22.
    [42]
    WANG P X, ZENG H L, LIN S L, et al. Anti-fatigue activities of hairtail(Trichiurus lepturus) hydrolysate in an endurance swimming mice model[J]. Funct Foods,2020:74.
    [43]
    YANG D K, LEE S J, ADAM G O, et al. Aralia continentalis kitagawa extract attenuates the fatigue induced by exhaustive exercise through inhibition of oxidative stress[J]. Antioxidants,2020,9:379. doi: 10.3390/antiox9050379
    [44]
    ZHONG L, MA N, ZHENG H H, et al. Tuber indicum polysaccharide relieves fatigue by regulating gut microbiota in mice[J]. Funct Foods,2019,63:103580. doi: 10.1016/j.jff.2019.103580
    [45]
    WAN J J, QIN Z, LEI H, et al. Erythromycin has therapeutic efficacy on muscle fatigue acting specifically on orosomucoid to increase muscle bioenergetics and physiological parameters of endurance[J]. Pharmacol Res,2020,161:105118. doi: 10.1016/j.phrs.2020.105118
    [46]
    孙熙浛, 齐欣, 崔承弼. 辐照红景天乙醇提取物的抗氧化作用及美白作用研究[J]. 食品工业科技,2020,41(23):325−331. [SUN X H, QI X, CUI C B. Study on the antioxidant and whitening effects of irradiated ethanol extract of Rhodiola rosea[J]. Food Industry Science and Technology,2020,41(23):325−331.
    [47]
    ZHANG C J, GUO J Y, CHENG H, et al. Spatial structure and anti-fatigue of polysaccharide from Inonotus obliquus[J]. International Journal of Biological Macromolecules,2020,151:855−860. doi: 10.1016/j.ijbiomac.2020.02.147
  • Cited by

    Periodical cited type(6)

    1. 余厚美,林立铭,王琴飞,姚庆群,杜培旭,张金泉,张振文. 不同酶解工艺对鲜木薯块根粗多糖得率的影响. 热带作物学报. 2025(02): 407-419 .
    2. 王焕燃,朱轶龙,孙萍. 白块菌多糖理化特性分析及对运动疲劳小鼠肝脏氧化损伤的保护作用. 现代食品科技. 2024(03): 56-64 .
    3. 陈盛宇,张淑梅,王玉霞,姜威,潘钰,马俊秀,叶阳,韩增华. 桦褐孔菌菌丝体多糖除杂工艺及对α-葡萄糖苷酶抑制作用的影响. 菌物研究. 2024(03): 268-275 .
    4. 刘艺珠,宋冠洁,郭苗苗,易帆,李丽. 桦树茸活性成分及药理活性研究进展. 中医药导报. 2023(01): 96-104 .
    5. 张锐,白海军,刘佳. 松杉灵芝多糖结构分析及对小鼠运动性疲劳的缓解作用. 中国食品添加剂. 2023(04): 69-78 .
    6. 黄小倩,李佳琪,孙家会,夏光华. 多糖的修饰及其改善乳化性能的研究进展. 食品工业科技. 2023(09): 437-445 . 本站查看

    Other cited types(2)

Catalog

    Article Metrics

    Article views (239) PDF downloads (20) Cited by(8)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return