Establishment of an Indirect Competitive ELISA for Zearalenone

TANG Song; LI Yansong; SHANG Cuiling; HU Pan; LU Shiying; REN Honglin; LIU Zengshan; ZHOU Yu

doi:10.13386/j.issn1002-0306.2021050177

Volume 43 Issue 4

Feb. 2022

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Science and Technology of Food Industry > 2022 > 43(4): 300-304. > DOI: 10.13386/j.issn1002-0306.2021050177

TANG Song, LI Yansong, SHANG Cuiling, et al. Establishment of an Indirect Competitive ELISA for Zearalenone[J]. Science and Technology of Food Industry, 2022, 43(4): 300−304. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021050177.

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Establishment of an Indirect Competitive ELISA for Zearalenone

1.
Institute of Zoonosis, Jilin University, Changchun 130062, China
2.
College of Animal Science, Yangtze University, Jingzhou 434023, China

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Received Date: May 20, 2021
Accepted Date: December 07, 2021
Available Online: December 16, 2021

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Abstract

Abstract

In this study, an indirect competitive ELISA (ic-ELISA) was developed for the detection of zearalenone in corn flour. The optimal working concentration of antigen and monoclonal antibody was determined by chessboard method. Antigen 37 ℃ coated for 1 h, unsealed and enzyme catalyzed substrate action time of 15 min were determined the optimal conditions. The results showed that the detection range (IC₂₀~IC₈₀) was 11~292 pg/mL, and the minimum detection limit (IC₁₀) was 6 pg/mL. The recoveries of the zearalenone from corn flour were 81.29%~105.80%. There was no cross reaction with ochratoxin A, aflatoxin B₁, deoxynivalenol, fumonisin B₁, T-2 toxin. The ic-ELISA could be used for detection of zearalenon in corn flour.
- zearalenone,
- toxin,
- detection,
- indirect competitive enzyme linked immunosorbent assay (ic-ELISA)

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References (22)

References

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