Optimization of Liquid Fermentation Process for Preparation of Silkworm Pupa Peptide by <i>Bacillus natto</i> and Its Anti-inflammatory Activity

SHEN Yuanyuan; YU Futian; QIN Yali; CHEN Shangli; DONG Shiyu; ZHAO Xiaoying; LIU Xiaoling

doi:10.13386/j.issn1002-0306.2021050156

Volume 43 Issue 3

Jan. 2022

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Science and Technology of Food Industry > 2022 > 43(3): 162-171. > DOI: 10.13386/j.issn1002-0306.2021050156

SHEN Yuanyuan, YU Futian, QIN Yali, et al. Optimization of Liquid Fermentation Process for Preparation of Silkworm Pupa Peptide by Bacillus natto and Its Anti-inflammatory Activity[J]. Science and Technology of Food Industry, 2022, 43(3): 162−171. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021050156.

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Optimization of Liquid Fermentation Process for Preparation of Silkworm Pupa Peptide by Bacillus natto and Its Anti-inflammatory Activity

College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, China

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Received Date: May 19, 2021
Available Online: December 02, 2021

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Abstract

Abstract

In order to improve the utilization rate of silkworm pupa resources and the added value of products, silkworm pupa peptides were prepared from silkworm pupa proteins by liquid fermentation of natto. The optimum fermentation conditions were determined by single-factor experiment and response surface methodology. The inflammatory model of RAW264.7 macrophages was established by lipopolysaccharide, and the anti-inflammatory activity of silkworm pupa peptides were studied in vitro. The results showed that the optimum fermentation conditions of silkworm pupa peptide were inoculation amount 5.0 mL, silkworm pupa protein addition amount 2.6 g, initial pH7.0, fermentation temperature 37 ℃ and fermentation time 35.4 h. Under these conditions, the yield of silkworm pupa peptide was 14.58%. Silkworm pupa peptides were nontoxic to RAW264.7 macrophages and had significant proliferation effect on cells (P<0.05). Silkworm pupa peptides could significantly inhibit the secretion of NO, IL-1β and IL-6 in the supernatant of RAW264.7 cells induced by LPS in a dose-dependent manner (P<0.05). This study provides a theoretical basis for the high value utilization of silkworm pupa and the research and development of food-derived anti-inflammatory peptides.
- silkworm pupa,
- Bacillus natto,
- liquid fermentation,
- peptide,
- anti-inflammatory activity

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