Synergistic Moderating Effects of L-Theanine and EGCG for the Prevention of Obesity and Hypercholesterolemia

LIU Baogui; CHEN Zhiyin; ZHANG Yangling; LIU Enshuo; HAN Shumin; XIAO Wenjun

doi:10.13386/j.issn1002-0306.2021050056

Volume 43 Issue 3

Jan. 2022

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Science and Technology of Food Industry > 2022 > 43(3): 341-350. > DOI: 10.13386/j.issn1002-0306.2021050056

LIU Baogui, CHEN Zhiyin, ZHANG Yangling, et al. Synergistic Moderating Effects of L-Theanine and EGCG for the Prevention of Obesity and Hypercholesterolemia[J]. Science and Technology of Food Industry, 2022, 43(3): 341−350. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021050056.

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Synergistic Moderating Effects of L-Theanine and EGCG for the Prevention of Obesity and Hypercholesterolemia

Key Lab of Tea Science, Ministry of Education, National Research Center of Engineering Technology for Utilization Ingredients from Botanicals,Co-innovation Center of Education Ministry for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, China

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Received Date: May 09, 2021
Available Online: November 30, 2021

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Abstract

Abstract

Based on the high-fat diet of successfully modeled obese and hypercholesterolemic rats, using simvastatin as a positive control, the results of epigallocatechin gallate (EGCG, 83.3 mg·kg⁻¹·d⁻¹) + L-Theanine (16.7 mg·kg⁻¹·d⁻¹), EGCG (100 mg·kg⁻¹·d⁻¹) and L-theanine (100 mg·kg⁻¹·d⁻¹) continuously fed by gavage to high-fat diet SPF-grade SD rats for 56 d were compared to investigate the synergistic moderating effects of L-theanine and EGCG for the prevention of obesity and hypercholesterolemia. The results showed that compared with the normal group, there were significant differences (P<0.05) in body weight and blood lipid level in the high-fat diet group, and the body weight had exceeded 21.2% of the weight of the normal group, and the total serum cholesterol exceeded 79.9% of the normal group, indicating that the obesity and hypercholesterolemia model rats were successfully modeled. Compared with the model group, all intervention groups could effectively inhibit the increase of body weight and blood lipid level in rats on long-term high-fat diet; compared with the L-theanine group, the combined EGCG+L-theanine intervention group significantly improved body weight, blood lipid, liver lipid and liver redox status (P<0.05). The combined EGCG+L-theanine intervention group showed better effects on body weight, abdominal fat mass, liver coefficient, organ index, hepatic malondialdehyde content, hepatic peroxidase activity, serum Apo B100 content, Apo B100/A1 ratio, and hepatic lipid accumulation compared to the EGCG group. Compared with the simvastatin group, only the combined EGCG+L-theanine intervention group showed a significant reduction in body weight, abdominal fat mass, and liver TG content (P<0.05); meanwhile, the pathological sections showed a large accumulation of lipids in tissues and organs of rats with long-term high-fat diet-induced obesity and hypercholesterolemia, which was significantly improved in the combined EGCG+L-theanine intervention group. This suggests that L-theanine and EGCG have synergistic regulatory effects in the prevention of obesity and hypercholesterolemia.
- L-theanine,
- epigallocatechin gallate,
- nutritional intervention,
- obesity,
- hypercholesterolemia,
- synergistic regulation

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References

[1]	ZHOU J, MAO L, XU P, et al. Effects of (-)-epigallocatechin gallate(EGCG) on energy expenditure and microglia-mediated hypothalamic inflammation in mice fed a high-fat diet[J]. Nutrients,2018,10(11):1681. doi: 10.3390/nu10111681
[2]	THOMPSON P D, PANZA G, ZALESKI A, et al. Statin-associated side effects[J]. J Am Coll Cardiol,2016,67(20):2395−2410. doi: 10.1016/j.jacc.2016.02.071
[3]	LANDINI L, REBELOS E, HONKA M-J. Green tea from the far east to the drug store: Focus on the beneficial cardiovascular effects[J]. Curr Pharm Des,2021,27(16):1931−1940. doi: 10.2174/1381612826666201102104902
[4]	KAROLCZAK D, SEGET M, BAJERSKA J, et al. Green tea extract prevents the development of nonalcoholic liver steatosis in rats fed a high-fat diet[J]. Pol J Pathol,2019,70(4):295−303. doi: 10.5114/pjp.2019.93132
[5]	ROBERTS J D, WILLMOTT A G B, BEASLEY L, et al. The impact of decaffeinated green tea extract on fat oxidation, body composition and cardio-metabolic health in overweight, recreationally active individuals[J]. Nutrients,2021,13(3):764. doi: 10.3390/nu13030764
[6]	BAGHERI R, RASHIDLAMIR A, ASHTARY-LARKY D, et al. Does green tea extract enhance the anti-inflammatory effects of exercise on fat loss?[J]. Br J Clin Pharmacol,2020,86(4):753−762. doi: 10.1111/bcp.14176
[7]	PENG W Q, XIAO G, LI B Y, et al. L-theanine activates the browning of white adipose tissue through the AMPK/α-Ketoglutarate/Prdm16 axis and ameliorates diet-induced obesity in mice[J]. Diabetes,2021,70(7):1458−1472. doi: 10.2337/db20-1210
[8]	ZHENG G, SAYAMA K, OKUBO T, et al. Anti-obesity effects of three major components of green tea, catechins, caffeine and theanine, in mice[J]. In Vivo,2004,18(1):55−62.
[9]	BASU A, SANCHEZ K, LEYVA M J, et al. Green tea supplementation affects body weight, lipids, and lipid peroxidation in obese subjects with metabolic syndrome[J]. J Am Coll Nutr,2010,29(1):31−40. doi: 10.1080/07315724.2010.10719814
[10]	YAN Q, TONG H, TANG S, et al. L-theanine administration modulates the absorption of dietary nutrients and expression of transporters and receptors in the intestinal mucosa of rats[J]. Biomed Res Int,2017:2017. doi: 10.1155/2017/9747256
[11]	KAO Y H, CHANG H H, LEE M J, et al. Tea, obesity, and diabetes[J]. Molecular Nutrition & Food Research,2006,50(2):188−210.
[12]	BOSE M, LAMBERT J D, JU J, et al. The major green tea polyphenol, (-)-epigallocatechin-3-gallate, inhibits obesity, metabolic syndrome, and fatty liver disease in high-fat-fed mice[J]. J Nutr,2008,138(9):1677−1683. doi: 10.1093/jn/138.9.1677
[13]	FRIEDRICH M, PETZKE K J, RAEDERSTORFF D, et al. Acute effects of epigallocatechin gallate from green tea on oxidation and tissue incorporation of dietary lipids in mice fed a high-fat diet[J]. Int J Obes(Lond),2012,36(5):735−743. doi: 10.1038/ijo.2011.136
[14]	SAE-TAN S, GROVE K A, KENNETT M J, et al. (-)-Epigallocatechin-3-gallate increases the expression of genes related to fat oxidation in the skeletal muscle of high fat-fed mice[J]. Food Funct,2011,2(2):111−116. doi: 10.1039/c0fo00155d
[15]	彭影琦, 袁冬寅, 林玲, 等. 表没食子儿茶素没食子酸酯对L-茶氨酸调节小鼠血清及肠道游离氨基酸的影响[J]. 食品科学,2020,41(19):154−160. [PENG Y Q, YUAN D Y, LIN L, et al. Effects of epigallocatechin gallate on the regulation of serum and intestinal free amino acids by L-theanine in mice[J]. Food Science,2020,41(19):154−160. doi: 10.7506/spkx1002-6630-20190829-325
[16]	刘晓慧, 揭国良, 林康, 等. EGCG和茶氨酸对细胞氧化损伤的协同保护和修复作用研究[J]. 茶叶科学,2014,34(3):239−247. [LIU X H, JIE G L, LIN K, et al. Study on the synergistic protective and repair effects of EGCG and theanine on cellular oxidative damage[J]. Tea Science,2014,34(3):239−247. doi: 10.3969/j.issn.1000-369X.2014.03.007
[17]	LIN L, ZENG L, LIU A, et al. L-theanine regulates glucose, lipid, and protein metabolism via insulin and AMP-activated protein kinase signaling pathways[J]. Food Funct,2020,11(2):1798−1809. doi: 10.1039/C9FO02451D
[18]	CHEN C, LIU Q, LIU L, et al. Potential biological effects of (-)-epigallocatechin-3-gallate on the treatment of nonalcoholic fatty liver disease[J]. Molecular Nutrition & Food Research,2018,62(1):1700483.
[19]	LI F, GAO C, YAN P, et al. EGCG reduces obesity and white adipose tissue gain partly through AMPK activation in mice[J]. Front Pharmacol,2018:9. doi: 10.3389/fphar.2018.01366
[20]	李秀平, 欧阳建, 唐静怡, 等. 茯砖茶通过调节肠道菌群和胆汁酸代谢预防肥胖及高胆固醇血症作用[J/OL]. 食品科学: 1−18 [2021-08-04]. http://kns.cnki.net/kcms/detail/11.2206.TS.20210207.1704.012.html. LI X P, OUYANG J, TANG J Y, et al. Preventing obesity and hypercholesterolemia by regulating intestinal flora and bile acid metabolism with Porphyra tea[J/OL]. Food Science: 1−18 [2021-08-04]. http://kns.cnki.net/kcms/detail/11.2206.TS.20210207.1704.012.html.
[21]	CHANDLER P C, VIANA J B, OSWALD K D, et al. Feeding response to melanocortin agonist predicts preference for and obesity from a high-fat diet[J]. Physiology & Behavior,2005,85(2):221−230.
[22]	诸骏仁, 高润霖, 赵水平, 等. 中国成人血脂异常防治指南(2016年修订版)[J]. 中国循环杂志,2016,31(10):937−953. [ZHU J R, GAO R L, ZHAO S P, et al. Guidelines for the prevention and treatment of dyslipidemia in Chinese adults (2016 revised edition)[J]. Chinese Journal of Circulation,2016,31(10):937−953. doi: 10.3969/j.issn.1000-3614.2016.10.001
[23]	李秀平, 欧阳建, 周方, 等. 茶叶功能成分预防心血管疾病研究进展[J]. 食品科学,2020,41(21):311−320. [LI X P, OUYANG J, ZHOU F, et al. Advances in the study of functional components of tea for the prevention of cardiovascular diseases[J]. Food Science,2020,41(21):311−320. doi: 10.7506/spkx1002-6630-20191015-137
[24]	王静, 黄庆辰, 高敏玥, 等. 连翘叶连翘苷提取物对高脂饮食诱导大鼠肥胖的预防作用[J]. 食品科学,2021,42(9):85−90. [WANG J, HUANG Q C, GAO M Y, et al. Preventive effect of forsythia leaf coniferin extract on high-fat diet-induced obesity in rats[J]. Food Science,2021,42(9):85−90. doi: 10.7506/spkx1002-6630-20201216-198
[25]	SCHEJA L, HEEREN J. Metabolic interplay between white, beige, brown adipocytes and the liver[J]. J Hepatol,2016,64(5):1176−1186. doi: 10.1016/j.jhep.2016.01.025
[26]	SCHNABL K, LI Y G, KLINGENSPOR M. The gut hormone secretin triggers a gut-brown fat-brain axis in the control of food intake[J]. Experimental Physiology,2020,105(8):1206−1213. doi: 10.1113/EP087878
[27]	汤磊乐, 黄卓山, 郑坚锐, 等. 载脂蛋白A1和载脂蛋白B100在冠脉病变进展中的作用[J]. 中山大学学报(医学科学版),2017,38(3):395−400. [TANG L L, HUANG Z S, ZHENG J R, et al. Role of apolipoprotein A1 and apolipoprotein B100 in the progression of coronary lesions[J]. Journal of Sun Yat-sen University (Medical Science Edition),2017,38(3):395−400.
[28]	YANG Z, ZHU M-Z, ZHANG Y-B, et al. Coadministration of epigallocatechin-3-gallate(EGCG) and caffeine in low dose ameliorates obesity and nonalcoholic fatty liver disease in obese rats[J]. Phytother Res,2019,33(4):1019−1026. doi: 10.1002/ptr.6295
[29]	PAIVA L, LIMA E, MOTTA M, et al. Variability of antioxidant properties, catechins, caffeine, l-theanine and other amino acids in different plant parts of Azorean[J]. Curr Res Food Sci,2020,3:227−234. doi: 10.1016/j.crfs.2020.07.004
[30]	SUGIYAMA T, SADZUKA Y. Enhancing effects of green tea components on the antitumor activity of adriamycin against M5076 ovarian sarcoma[J]. Cancer Letters,1998,133(1):19−26. doi: 10.1016/S0304-3835(98)00185-2
[31]	SUGIYAMA T, SADZUKA Y. Combination of theanine with doxorubicin inhibits hepatic metastasis of M5076 ovarian sarcoma[J]. Clinical Cancer Research,1999,5(2):413−416.
[32]	SUN L, TIAN X, GOU L, et al. Beneficial synergistic effects of concurrent treatment with theanine and caffeine against cerebral ischemia-reperfusion injury in rats[J]. Canadian Journal of Physiology & Pharmacology,2013,91(7):562−569.
[33]	LEE H, BAE S, YOON Y. The anti-adipogenic effects of (-)epigallocatechin gallate are dependent on the WNT/β-catenin pathway[J]. J Nutr Biochem,2013,24(7):1232−1240. doi: 10.1016/j.jnutbio.2012.09.007

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