Extraction, Purification, Identification of A-type Procyanidine from Litchi Fruitlet and Its Antioxidant Activity

XIE Chong; LIN Lin; WU Geyi; WANG Kai; HU Zhuoyan; ZHAO Lei

doi:10.13386/j.issn1002-0306.2021050048

Volume 43 Issue 3

Jan. 2022

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Science and Technology of Food Industry > 2022 > 43(3): 81-87. > DOI: 10.13386/j.issn1002-0306.2021050048

XIE Chong, LIN Lin, WU Geyi, et al. Extraction, Purification, Identification of A-type Procyanidine from Litchi Fruitlet and Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2022, 43(3): 81−87. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021050048.

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Extraction, Purification, Identification of A-type Procyanidine from Litchi Fruitlet and Its Antioxidant Activity

College of Food Science, South China Agricultural University, Guangzhou 510642, China

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Received Date: May 09, 2021
Available Online: November 29, 2021

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Abstract

Abstract

In this study, polyphenol fractions were extracted from litchi fruitlet which drop naturally, then purified by AB-8 macroporous resin and separated by Sephadex LH-20. After comparing the antioxidant activities of each sample in vitro, the polyphenol components were identified by LC-ESI-Q-TOF. The results showed that the content of total phenols and total procyanidines in litchi fruitlet were (49.96±2.66) mg GAE/g DW and (27.25±0.44) mg EPE/g DW, respectively. In vitro antioxidant activity text indicated that the fourth polyphenol component (P4) had strong ABTS⁺· and DPPH· scavenging ability, and its free radical scavenging rate could reach 92.52% and 46.98% in 100 μg/mL respectively. According to LC-ESI-Q-TOF analysis, PLFE contained 10 types of A procyanidines, which were much more than B procyanidines. P4 was composed of pure A-type procyanidine, which were procyanidine A₄, procyanidine A₂, A-type procyanidine dimer and tetramer. Therefore, A-type procyanidine isolated and purified from litchi fruitlet have remarkable antioxidant activity, which can be used as an excellent source of new A-type procyanidine.
- litchi,
- A-type procyanidine,
- antioxidant activity,
- isolation and purification,
- structure identification

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