Effects of Free Phenolics from <i>Lycopus lucidus</i> Turcz. on Apoptosis, Cell Cycle and Inflammatory Factors in CCl<sub>4</sub> Induced Hepatocyte Injury

LI Yuanli; HONG Yue; KONG Xiao; LIN Lin; ZHANG Tianyang; LI Wang; LIU Qing; GAO Chunyan; LU Yuehong

doi:10.13386/j.issn1002-0306.2021050035

Volume 43 Issue 3

Jan. 2022

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Science and Technology of Food Industry > 2022 > 43(3): 333-340. > DOI: 10.13386/j.issn1002-0306.2021050035

LI Yuanli, HONG Yue, KONG Xiao, et al. Effects of Free Phenolics from Lycopus lucidus Turcz. on Apoptosis, Cell Cycle and Inflammatory Factors in CCl₄ Induced Hepatocyte Injury[J]. Science and Technology of Food Industry, 2022, 43(3): 333−340. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021050035.

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Effects of Free Phenolics from Lycopus lucidus Turcz. on Apoptosis, Cell Cycle and Inflammatory Factors in CCl₄ Induced Hepatocyte Injury

1.
College of Public Health, Dali University, Dali 671000, China
2.
Dali Bai Autonomous Prefecture Chinese Medicine Hospital, Dali 671000, China
3.
College of Biological Science and Engineering , North Minzu University, Yinchuan 750021, China

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Received Date: May 07, 2021
Available Online: December 06, 2021

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Abstract

Abstract

To investigate the effects of free phenolics from Lycopus lucidus Turcz. on apoptosis, cell cycle and inflammatory factors in CCl₄ induced hepatocyte injury by using a in vitro experiment. Apoptosis and the distribution of cell cycle was analyzed by flow cytometry. The contents of tumor necrosis factor-α (TNF-α), interleukin-8 (IL-8) and the activation of Caspase-3 were measured by kits. The protein expression levels of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and interleukin-6 (IL-6) were also detected by immunohistochemistry. The results showed that compared with the model group, the free phenolics from Lycopus lucidus Turcz. could significantly reduce the apoptosis induced by CCl₄ and affect the distribution of cell cycle. In addition, the free phenolics from Lycopus lucidus Turcz. decreased the contents of TNF-α and IL-8 and blocked the activation of Caspase-3 (P<0.05). The free phenolics from Lycopus lucidus Turcz. also decreased the expression of COX-2, IL-6 and iNOS (P<0.05). The free phenolics from Lycopus lucidus Turcz. could protect the CCl₄ induced hepatocyte injury through inhibiting apoptosis, affecting cell cycle distribution and inhibiting inflammatory reaction.
- Lycopus lucidus Turcz.,
- free phenolics,
- carbon tetrachloride,
- hepatocyte injury,
- cell cycle,
- inflammatory factors

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