Structure Characterization and Antioxidant Activity of Polysaccharides from <i>Amomum villosum</i> Extracted with Alkaline Solution

WEI Zhi; RUAN Xinmei; DAI Taotao; FU Min; YUAN Jiancheng; LI Ti; CHEN Jun

doi:10.13386/j.issn1002-0306.2021040255

Volume 42 Issue 24

Dec. 2021

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Science and Technology of Food Industry > 2021 > 42(24): 87-93. > DOI: 10.13386/j.issn1002-0306.2021040255

WEI Zhi, RUAN Xinmei, DAI Taotao, et al. Structure Characterization and Antioxidant Activity of Polysaccharides from Amomum villosum Extracted with Alkaline Solution[J]. Science and Technology of Food Industry, 2021, 42(24): 87−93. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021040255.

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Structure Characterization and Antioxidant Activity of Polysaccharides from Amomum villosum Extracted with Alkaline Solution

1.
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
2.
Guangxi Key Laboratory of Fruits and Vegetables Storage-processing Technology, Guangxi Academy of Agricultural Sciences, Nanning 530007, China

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Received Date: April 25, 2021
Available Online: October 13, 2021

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Abstract

Abstract

In this study, A. villosum was used as the raw material, and 0.1 mol/L NaOH alkaline solution was used as extraction solution to prepare A. villosum polysaccharides. The composition, structure and antioxidant activity in vitro were analyzed. The results showed that, the extraction rate of polysaccharides was 3.97%, the total sugar content was 37.10%, the uronic acid content was 65.66%, the protein content was 4.36%, and the molecular weight of polysaccharide was 95.46 kDa. The results of high-performance liquid chromatography showed that A. villosum polysaccharides were mainly composed of arabinose, xylose, galactose and galacturonic acid. Fourier transform infrared spectroscopy(FTIR), scanning electron microscopy(SEM), X-ray diffraction(XRD), thermogravimetric analysis(TGA) results showed that it was an amorphous structure, had a pyran ring, and thermally stable sheet-like acidity heteropolysaccharides. The results of in vitro antioxidant models(DPPH, ABTS⁺, hydroxyl free radicals) showed that the polysaccharides of A. villosum had good in vitro antioxidant capacity. This research aimed to provide a theoretical basis for the application andproduct development of polysaccharides from A. villosum.
- Amomum villosum,
- polysaccharide,
- alkaline extraction,
- chemical components,
- structural,
- antioxidant activity

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