WANG Yan, YUAN Hongbo, DONG Yingying, et al. Analysis on Research Status of Triterpenoids in Sporophore of Inonotus obliquus[J]. Science and Technology of Food Industry, 2022, 43(9): 416−422. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021040158.
Citation: WANG Yan, YUAN Hongbo, DONG Yingying, et al. Analysis on Research Status of Triterpenoids in Sporophore of Inonotus obliquus[J]. Science and Technology of Food Industry, 2022, 43(9): 416−422. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021040158.

Analysis on Research Status of Triterpenoids in Sporophore of Inonotus obliquus

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  • Received Date: April 14, 2021
  • Available Online: February 27, 2022
  • Inonotus obliquus is anatural plant fungal resource with a variety of biological activities. Triterpenoids are the main functional components. Because triterpenoids have excellent anticancer, hypoglycemic and antioxidant properties, the related research and development has attracted much attention in recent years. In this paper, the current research status of Inonotus obliquus triterpenoid compounds are summarized and analyzed from the aspects of induction fermentation production, separation and purification of sporophore triterpenoid compounds, chemical composition and structure analysis, functional characteristics and application development, among which the use of plant commensal microbial extracts as inducers in induction fermentation production is the most significant. The separation and purification techniques have respective advantages according to different purposes. The separation and purification technologies with high separation efficiency such as high-performance counter-current chromatography and high-performance liquid chromatography are more suitable for chemical composition and structure analysis, and macroporous resin has more advantages in large-scale application. Based on the above analysis, the future research direction is prospected, which provides a theoretical basis for subsequent development and research and application in that medicine and food industry.
  • [1]
    戴玉成, 李玉. 中国六种重要药用真菌名称的说明[J]. 菌物学报,2011,30(4):515−518. [DAI Yucheng, LI Yu. Notes on the nomenclature of six important medicinal fungi in China[J]. Mycosystema,2011,30(4):515−518.
    [2]
    李艳婷, 郭尚, 徐莉娜, 等. 桦褐孔菌资源分布及其地域环境条件分析[J]. 中国林副特产,2019(4):60−65. [LI Yanting, GUO Shang, XU Lina, et al. Distribution of Inonotus obliquus resources and analysis of its regional environmental conditions[J]. Forest By-Product and Speciality in China,2019(4):60−65.
    [3]
    齐亭娟, 周玉柏, 曾毅. 桦褐孔菌活性成分及药理作用的研究进展[J]. 智慧健康,2018,4(24):50−53. [QI Tingjuan, ZHOU Yubai, ZENG Yi. Research progress on active constituents and pharmacological action of betula brown-hole bacteria[J]. Smart Healthcare,2018,4(24):50−53.
    [4]
    梁清乐, 王秋颖, 樊锦燕, 等. 桦褐孔菌的研究概况[J]. 中草药,2005(4):623−625. [LIANG Qingle, WANG Qiuying, FAN Jinyan, et al. A survey of Inonotus obliquus[J]. Chinese Traditional and Herbal Drugs,2005(4):623−625. doi: 10.3321/j.issn:0253-2670.2005.04.053
    [5]
    马微, 谭阳阳, 魏书娟, 等. 俄罗斯桦褐孔菌中无机元素含量分析[J]. 食品与机械,2020,36(12):34−38,135. [MA Wei, TAN Yangyang, WEI Shujuan, et al. Analysis of inorganic elements in Russian Inonotus obliquus[J]. Food & Machinery,2020,36(12):34−38,135.
    [6]
    孙嵘. 桦褐孔菌的食用安全性评价[D]. 长春: 吉林农业大学, 2019.

    SUN Rong. Evaluation of edible safety of Inonotus obliquus[D]. Changchun: Jilin Agricultural University, 2019.
    [7]
    贺紫薇, 刘旭, 李东辉, 等. 桦褐孔菌研究进展[J]. 中医药信息,2020,37(2):119−123. [HE Ziwei, LIU Xu, LI Donghui, et al. Research progress of Inonotus obliquus[J]. Chinese Medicine Information,2020,37(2):119−123.
    [8]
    杨宏博, 韩增华, 杨红, 等. 诱导发酵桦褐孔菌三萜类化合物合成及其抗氧化功能的研究[J]. 食品工业科技,2020,41(13):105−111. [YANG Hongbo, HAN Zenghua, YANG Hong, et al. Study on the synthesis and antioxidant function of triterpenes from Inonotus obliquus induced by fermentation[J]. Science and Technology of Food Industry,2020,41(13):105−111.
    [9]
    管玉艳, 王琨, 左松, 等. 外源因子对桦褐孔菌发酵产桦褐孔菌醇的影响[J]. 菌物学报,2020,39(7):1368−1379. [GUAN Yuyan, WANG Kun, ZUO Song, et al. Effects of elicitors on the production of inotodiol by submerged fermentation of Inonotus obliquus[J]. Mycosystema,2020,39(7):1368−1379.
    [10]
    LIN P, YAN Z F, LI C T. Effects of exogenous elicitors on triterpenoids accumulation and expression of farnesyl diphosphate synthase gene in Inonotus obliquus[J]. Biotechnology and Bioprocess Engineering,2020,25(4):580−588. doi: 10.1007/s12257-019-0502-y
    [11]
    XU X, ZHANG X, CHEN C. Stimulated production of triterpenoids of Inonotus obliquus using methyl jasmonate and fatty acids[J]. Industrial Crops and Products,2016,85:49−57. doi: 10.1016/j.indcrop.2016.02.046
    [12]
    刘爽爽. 粗毛纤孔菌总三萜的诱导合成、纯化及降血脂作用研究[D]. 哈尔滨: 东北林业大学, 2020.

    LIU Shuangshuang. Synthesis, purification and hypolipidemic effect of total triterpenoids from Inonotus hispidus[D]. Harbin: Northeast Forestry University, 2020.
    [13]
    檀琪. 桦褐孔菌三萜类化合物的提取纯化及抗氧化活性研究[D]. 太原: 山西医科大学, 2020.

    TAN Qi. Extraction, purification and antioxidant activity of triterpenoids from Inonotus obliguus[D]. Taiyuan: Shanxi Medical University, 2020.
    [14]
    胡涛, 王亚亚, 路春桃, 等. 超声波辅助提取桦褐孔菌子实体中三萜类化合物的研究[J]. 安徽农业科学,2011,39(35):21634−21635, 21643. [HU Tao, WANG Yaya, LU Chuntao, et al. Study on the ultrasonic assisted extraction technology of triterpenoid from Inonotus obliquus[J]. Journal of Anhui Agricultural Sciences,2011,39(35):21634−21635, 21643. doi: 10.3969/j.issn.0517-6611.2011.35.028
    [15]
    崔彦如. 桦褐孔菌有效成分的PEF提取、化学修饰和抗癌活性的研究[D]. 吉林: 吉林大学, 2008

    CUI Yanru. Active components extraction from Inouotus Obliquus, PEF and its chemical modification and anticancer activities[D]. Jilin: Jilin University, 2008.
    [16]
    赵佳敏, 陈泓鑫, 林宇冲, 等. 灵芝三萜的提取工艺及其抗氧化研究进展[J]. 安徽化工,2021,47(1):11−12,16. [ZHAO Jiamin, CHEN Hongxin, LIN Yuchong, et al. Research progress on extraction technology and antioxidation of triterpenes from Ganoderma lucidum[J]. Anhui Chemical Industry,2021,47(1):11−12,16. doi: 10.3969/j.issn.1008-553X.2021.01.004
    [17]
    赵艳霞, 刘峰, 魏娟娟, 等. 双水相超声法提取桦褐孔菌三萜[J]. 菌物学报,2014,33(1):129−137. [ZHAO Yanxia, LIU Feng, WEI Juanjuan, et al. Extraction of triterpenoids from Inonotus obliquus by aqueous two-phase systems associated with ultrasonic extraction[J]. Mycosystema,2014,33(1):129−137.
    [18]
    戚月婷, 玄光善. 桦褐孔菌总三萜提取工艺的研究[J]. 应用化工,2015,44(5):924−926, 932. [QI yueting, XUAN guangshan. Study on extraction process of total triterpenoids from Inonotus obliquus[J]. Applied Chemical Industry,2015,44(5):924−926, 932.
    [19]
    王干珍, 易晓明, 周林宗, 等. 高效逆流色谱原理及其研究进展[J]. 工业技术创新,2017,4(1):179−182. [WANG Ganzhen, YI Xiaoming, ZHOU Linzong, et al. Principle and research progress of high performance countercurrent chromatography[J]. Industrial Technology Innovation,2017,4(1):179−182.
    [20]
    刘延杰, 陈弯弯, 向碧云, 等. 硅胶柱层析分离天然产物实验要点分析[J]. 生物学通报,2018,53(6):44−46. [LIU Yanjie, CHEN Wanwan, XIANG Biyun, et al. Analysis of the experimental points of separating natural products by silica gel column chromatography[J]. Biology Bulletin,2018,53(6):44−46. doi: 10.3969/j.issn.0006-3193.2018.06.015
    [21]
    李广林. 桦褐孔菌三萜提取分离、结构鉴定及生物活性研究[D]. 杭州: 浙江工业大学, 2014.

    LI Guanglin. Extraction, isolation, structural identification and biological activity of triterpenoid from Inonotus obliquus[D]. Hangzhou: Zhejiang University of Technology, 2014.
    [22]
    吴战海. 高效液相色谱法在药品质量分析中的应用进展[J]. 黑龙江科技信息,2017(4):118. [WU Zhanhai. Application progress of high performance liquid chromatography in drug quality analysis[J]. Heilongjiang Science and Technology Information,2017(4):118.
    [23]
    檀琪, 阮文辉, 杨官娥, 等. 大孔吸附树脂纯化桦褐孔菌三萜类化合物工艺优化[J]. 食品工业,2021,42(3):142−146. [TAN Qi, RUAN Wenhui, YANG Guan'e, et al. Optimization of purification process of triterpenoids from Inonotus obliquus by macroporous adsorption resin[J]. Food Industry,2021,42(3):142−146.
    [24]
    胡涛, 黄美, 刘萍. 大孔吸附树脂分离纯化桦褐孔菌三萜工艺研究[J]. 食品科技,2012,37(4):206−210. [HU Tao, HUANG Mei, LIU Ping. Isolation and purification of triterpenes in Inonotus obliquus by macroporous resins[J]. Food Science and Technology,2012,37(4):206−210.
    [25]
    陈程, 徐向群. 桦褐孔菌子实体三萜单体的分离及鉴定[J]. 浙江理工大学学报,2015,33(3):264−268. [CHEN Cheng, XU Xiangqun. Isolation and identification of triterpenoids in Inonotus obliquus[J]. Journal of Zhejiang Sci-Tech University,2015,33(3):264−268.
    [26]
    董红敬. 茯苓皮三萜提取物化学成分及抗肿瘤活性研究[D]. 北京: 中国中医科学院, 2015.

    DONG Hongjing. Antitumor activities of triterpenes isolated from theepidermis of Poria cvcvs (Schw). Wolf[D]. Beijing: Chinese Academy of Traditional Chinese Medicine, 2015.
    [27]
    赵卓卓, 冉棋, 丁辉, 等. 桦褐孔菌醇研究进展[J]. 食用菌学报,2018,25(4):121−129. [ZHAO Zhuozhuo, RAN Qi, DING Hui, et al. Advances in inotodiol research[J]. Acta Edulis Fungi,2018,25(4):121−129.
    [28]
    王健铭, 李晓雪, 陈俊磊, 等. 桦褐孔菌三萜类化合物的研究[J]. 齐齐哈尔大学学报(自然科学版),2017,33(4):62−64. [WANG Jianming, LI Xiaoxue, CHEN Junlei, et al. Study on triterpenes of Inonotus obliquus[J]. Journal of Qiqihar University (Natural Science Edition),2017,33(4):62−64.
    [29]
    张仕瑾, 谢运飞, 谭玉柱, 等. 桦褐孔菌三萜类化学成分研究[J]. 中草药,2015,46(16):2355−2360. [ZHANG Shijin, XIE Yunfei, TAN Yuzhu, et al. Studies on triterpenoids from Inonotus obliquus[J]. Chinese Herbal Medicine,2015,46(16):2355−2360.
    [30]
    KIM J, YANG S C, HWANG A Y, et al. Composition of triterpenoids in Inonotus obliquus and their anti-proliferative activity on cancer cell lines[J]. Molecules (Basel, Switzerland),2020,25(18):425−432.
    [31]
    ALZAND K I, NAL S, BOUFARIS M. Lanostane-type triterpenes and abietane-type diterpene from the sclerotia of Chaga medicinal mushroom, Inonotus obliquus (agaricomycetes), and their biological activities[J]. International Journal of Medicinal Mushrooms,2018,20(6):58−65.
    [32]
    DURU K C, KOVALEVA E G, DANILOVA I G, et al. The pharmacological potential and possible molecular mechanisms of action of Inonotus obliquus from preclinical studies[J]. Phytotherapy Research,2019,33(8):1966−1980. doi: 10.1002/ptr.6384
    [33]
    PFEFFER C M, SINGH A T K. Apoptosis: A target for anticancer therapy[J]. International Journal of Molecular Sciences,2018,19(2):448. doi: 10.3390/ijms19020448
    [34]
    KIM J, YANG S C, HWANG A Y. Composition of triterpenoids in Inonotus obliquus and their anti-proliferative activity on cancer cell lines[J]. Molecules, 2020, 25(18): 4066.
    [35]
    BAEK J, ROH H S, BAEK K H, et al. Bioactivity-based analysis and chemical characterization of cytotoxic constituents from Chaga mushroom (Inonotus obliquus) that induce apoptosis in human lung adenocarcinoma cells[J]. Journal of Ethnopharmacology,2018,224:63−75. doi: 10.1016/j.jep.2018.05.025
    [36]
    ZHAO F, MAI Q, MA J, et al. Triterpenoids from Inonotus obliquus and their antitumor activities[J]. Fitoterapia,2015,101:34−40. doi: 10.1016/j.fitote.2014.12.005
    [37]
    金黎达, 欧文斌, 徐向群. 液体发酵桦褐孔菌三萜类成分抑制肿瘤细胞活性研究[J]. 浙江理工大学学报(自然科学版),2019,41(1):98−105. [JIN Lida, OU Wenbin, XU Xiangqun. Preliminary study on tumor cell activity inhibition effect of triterpene from submerged fermentation of Inonotus obliquus[J]. Journal of Zhejiang Sci-Tech University,2019,41(1):98−105.
    [38]
    MA Q, LI Y, WANG M, et al. Progress in metabonomics of type 2 diabetes mellitus[J]. Molecules,2018,23(7):1834. doi: 10.3390/molecules23071834
    [39]
    王秋爽. 桦褐孔菌对不同造模方法建立的2型糖尿病大鼠降血糖作用初探[D]. 延吉: 延边大学, 2017.

    WANG Qiushuang. A preliminary study on the hypoglycemic effects of Inonotus pbliquus of type 2 diabetic rats made by different methods[D]. Yanji: Yanbian University, 2017.
    [40]
    KUMAR S R, SINGH R, KUMAR D A. Important aspects of post-prandial antidiabetic drug, acarbose[J]. Current Topics in Medicinal Chemistry,2016,16(23):2625−2633. doi: 10.2174/1568026616666160414123500
    [41]
    黄盼盼, 陈程, 徐向群. 桦褐孔菌三萜对α-葡萄糖苷酶和α-淀粉酶的抑制活性及其有效成分鉴定[J]. 浙江理工大学学报(自然科学版),2020,43(5):678−686. [HUANG Panpan, CHEN Cheng, XU Xiangqun. Inhibitory activity of triterpenoids from Inonotus obliquus on α-glucosidase and α-amylase and identification of their effective compounds[J]. Journal of Zhejiang Sci-Tech University,2020,43(5):678−686.
    [42]
    CUI Y, KIM D S, PARK K C. Antioxidant effect of Inonotus obliquus[J]. Journal of Ethnopharmacology,2005,96(1-2):79−85. doi: 10.1016/j.jep.2004.08.037
    [43]
    赵芬琴, 严琳, 崔仙红, 等. 桦褐孔菌三萜对CCl4致小鼠氧化应激损伤的保护作用[J]. 药学学报,2012,47(5):680−684. [ZHAO Fenqin, YAN Lin, CUI Xianhong, et al. Triterpenoids from Inonotus obliquus protect mice against oxidative damage induced by CCl4[J]. Acta Pharmaceutica Sinica,2012,47(5):680−684.
    [44]
    许丽璇, 蔡建秀, 岳枝玲. 桦褐孔菌乙醇提取物对小鼠体内外抗氧化作用研究[J]. 中国食品学报,2012,12(6):48−55. [XU Lixuan, CAI Jianxiu, YUE Zhiling. Study on the antioxidant activity of ethanol crude extracts from mycelia of Inonotus obliquus[J]. Journal of Chinese Institute of Food Science and Technology,2012,12(6):48−55. doi: 10.3969/j.issn.1009-7848.2012.06.008
    [45]
    高愿军, 王娟娟, 龙娇妍, 等. 桦褐孔菌多酚在小鼠血清中的抗氧化作用研究[J]. 食品工业科技,2010,31(9):331−333. [GAO Yuanjun, WANG Juanjuan, LONG Jiaoyan, et al. Study on the antioxidation effect of Inonotus obliquus polyphenol on mice serum[J]. Science and Technology of Food Industry,2010,31(9):331−333.
    [46]
    吴艳玲, 南极星. 桦褐孔菌多糖对小鼠抗氧化清除自由基作用的研究[J]. 亚太传统医药,2009,5(12):9−10. [WU Yanling, NAN Jixing. Study on antioxidant and free radical scavenging effects of Inonotus obliquus polysaccharide in mice[J]. Asia-Pacific Traditional Medicine,2009,5(12):9−10.
    [47]
    ZOU C X, DONG S H, HOU Z L, et al. Modified lanostane-type triterpenoids with neuroprotective effects from the fungus Inonotus obliquus[J]. Bioorganic Chemistry,2020,105:104438. doi: 10.1016/j.bioorg.2020.104438
    [48]
    SAGAYAMA K, TANAKA N, FUKUMOTO T, et al. Lanostane-type triterpenes from the sclerotium of Inonotus obliquus (Chaga mushrooms) as proproliferative agents on human follicle dermal papilla cells[J]. Journal of Natural Medicines,2019,73(3):597−601. doi: 10.1007/s11418-019-01280-0
    [49]
    PARK S, SHIN H, PARK D, et al. Structure elucidation of a new triterpene from Inonotus obliquus[J]. Magnetic Resonance in Chemistry,2021,59(4):489−494. doi: 10.1002/mrc.5102
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