Active Proteins and Phospholipids Prepared from Chicken Egg Yolk by Green and Efficient Sequential Separation Technology

LIU Cong; ZHOU Xin; LUO Jinxu; XIA Minquan; LI Xiaomeng; WANG Chen; CAI Zhaoxia

doi:10.13386/j.issn1002-0306.2021040149

Volume 43 Issue 1

Dec. 2021

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Science and Technology of Food Industry > 2022 > 43(1): 180-187. > DOI: 10.13386/j.issn1002-0306.2021040149

LIU Cong, ZHOU Xin, LUO Jinxu, et al. Active Proteins and Phospholipids Prepared from Chicken Egg Yolk by Green and Efficient Sequential Separation Technology[J]. Science and Technology of Food Industry, 2022, 43(1): 180−187. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021040149.

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Active Proteins and Phospholipids Prepared from Chicken Egg Yolk by Green and Efficient Sequential Separation Technology

1.
College of Food Science and Technology, Huazhong Agricultural University, Key Laboratory of Environment Correlative Dietology, Ministry of Education, National and Local Joint Engineering Research Center for Egg Processing Technology, Wuhan 430070, China
2.
Gaoyou Qinyou Egg Products Co., Ltd., Yangzhou 225000, China

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Received Date: April 14, 2021
Available Online: November 04, 2021

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Abstract

Abstract

In order to achieve the full utilization of the functional components of egg yolk, a green solvent was used to separate bioactive immunoglobulin Y(IgY), phosvitin(PV), and phospholipids(PL) from fresh chicken egg yolk efficiently and continuously. Firstly, the water-soluble fraction was separated from the lipoproteins by water dilution(dilution ratio of 1:10), and the supernatant was salted by 35% saturated (NH₄)₂SO₄ and 0.5% NaCl(w/v) and IgY purified by 8% PEG 6000. The PV in the lipid fraction was further purified in NaCl solution(w/v) at pH5.0, 90 ℃. All lipids in egg yolk were extracted using an ethanol/ethyl acetate ratio of 1:2(v/v), and PL in total egg yolk lipid was further purified using ethyl acetate at 0 ℃. After desalting and freeze-drying, the purity of IgY, PV and PL was 97.38%, 78.63% and 85.94%, respectively. The yield of IgY and PV was 6.15 mg/mL egg yolk and 10.15 mg/mL egg yolk, respectively, and the content of PL in lipids accounted for 35.18%. The PL product may be served as a good source of dietetic applications due to its favorable PUFA/SFA ratio(0.70) and comparable lower n-6/n-3 ratio(5.37). These results proved that IgY, PV and PL can be purified in sequence from egg yolk with high purity. Also, this method is very simple, environment friendly and can provide technical support for the comprehensive utilization of egg yolk.
- egg yolk,
- bioactive components,
- co-extraction,
- ethyl acetate extraction,
- fatty acids

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References

[1]	WANG L H, LI X Y, JIN L J, et al. Characterization of chicken egg yolk immunoglobulins(IgYs) specific for the most prevalent capsular serotypes of mastitis-causing Staphylococcus aureus[J]. Veterinary Microbiology,2013,84(3-4):264−271. doi: 10.1016/j.vetmic.2010.11.029
[2]	OLIVER C, VALENZUELA K, SILVA H, et al. Effectiveness of egg yolk immunoglobulin against the intracellular salmonid pathogen Piscirickettsia salmonis[J]. Journal of Applied Microbiology,2015,119(2):365−376. doi: 10.1111/jam.12857
[3]	PEREIRA E P V, TILBURG M F V, FLOREAN E O P T, et al. Egg yolk antibodies(IgY) and their applications in human and veterinary health: A review[J]. International Immunopharmacology,2019,73(3):293−303.
[4]	CUI Y, LI X, LU M, et al. Role of polysaccharide conjugation in physicochemical and emulsifying properties of egg phosvitin and the calcium binding capacity of its phosphopeptides[J]. Food & Function,2019,10(4):1808−1815.
[5]	DUAN X, ZHOU Y, LI M, et al. Postfertilization changes in conformation of egg yolk phosvitin and biological activities of phosphopeptides[J]. Food Research International,2014,62:1008−1014. doi: 10.1016/j.foodres.2014.05.027
[6]	DUFFULER P, GIARRATANO M, NADERI N, et al. High hydrostatic pressure induced extraction and selective transfer of β-phosvitin from the egg yolk granule to plasma fractions[J]. Food Chemistry,2020,321(12):66−74.
[7]	GUO D, WAN P, LIU J, et al. Use of egg yolk phospholipids to boost the generation of the key odorants as well as maintain a lower level of acrylamide for vacuum fried French fries[J]. Food Control,2020,121:107592.
[8]	HOOGEVEST P V, TIEMESSEN H, METSELAAR J M, et al. The use of phospholipids to make pharmaceutical form line extensions[J]. European Journal of Lipid Science and Technology,2021,123(4):1−38.
[9]	WANG H, YAO L, LEE S L, et al. Simultaneous texturization and extraction of phospholipids from liquid egg yolk using renewable solvents[J]. European Journal of Lipid Science & Technology, 2017, 119(2).
[10]	TONG C, GENG F, HE Z, et al. A simple method for isolating chicken egg yolk immunoglobulin using effective delipidation solution and ammonium sulfate[J]. Poultry science,2015,94(1):104−110. doi: 10.3382/ps/peu005
[11]	KONDRATOWICZ A, NEUNERT G, NIEZGODA N, et al. Egg yolk extracts as potential liposomes shell material: Composition compared with vesicles characteristics[J]. Journal of Food Science,2018,83(10-12):2527−2535.
[12]	BERNARDO J, VIDEIRA R A, VALENTAO P, et al. Extraction of phospholipid-rich fractions from egg yolk and development of liposomes entrapping a dietary polyphenol with neuroactive potential[J]. Food and Chemical Toxicology,2019,133:110749−110749. doi: 10.1016/j.fct.2019.110749
[13]	张元来, 张兆旺. 水稀释法与辛酸法提取鸡卵黄抗体的比较[J]. 畜牧兽医杂志,2017,36(1):28−33. [ZHANG Y L, ZHANG Z W. Comparison of water dilution method and caprylic acid method of extracting yolk antibodies[J]. Journal of Animal Science and Veterinary Medicine,2017,36(1):28−33. doi: 10.3969/j.issn.1004-6704.2017.01.008
[14]	ZHOU X, WANG Y R, AHN D U, et al. An easy and simple separation method for Fc and Fab fragments from chicken immunoglobulin Y(IgY)[J]. Journal of Chromatography B,2020:1141.
[15]	TAN S H, MOHAMEDALI A, KAPUR A, et al. A novel, cost-effective and efficient chicken egg IgY purification procedure[J]. Journal of Immunological Methods,2012,380(1-2):73−76. doi: 10.1016/j.jim.2012.03.003
[16]	REDWAN E M, ALJADAWI A A, UVERSKY V N. Simple and efficient protocol for immunoglobulin Y purification from Chicken egg yolk[J]. Poultry Science, 2021, 100(3).
[17]	AKITA E M, NAKAI S. Immunoglobulins from egg-yolk-isolation and purification[J]. Journal of Food Science,1992,57:629−634. doi: 10.1111/j.1365-2621.1992.tb08058.x
[18]	REN H, YANG W J, THIRUMALAI D, et al. A comparative evaluation of six principal IgY antibody extraction methods[J]. Alternatives to Laboratory Animals,2016,44:11−20. doi: 10.1177/026119291604400111
[19]	KO K Y, AHN D U. Preparation of immunoglobulin Y from egg yolk using ammonium sulfate precipitation and ion exchange chromatography[J]. Poultry Science,2007,86(2):400−407. doi: 10.1093/ps/86.2.400
[20]	JIANG X, DIRAVIVAM T, ZHANG X. Affinity purification of egg yolk immunoglobulins(IgY) using a human mycoplasma protein[J]. Journal of Chromatography B,2016,1012-1013:37−41. doi: 10.1016/j.jchromb.2016.01.012
[21]	BALARAMAN H B, RATHNASAMY S K. High selective purification of IgY from quail egg: Process design and quantification of deep eutectic solvent based ultrasound assisted liquid phase microextraction coupled with preparative chromatography-ScienceDirect[J]. International Journal of Biological Macromolecules,2020,146:253−262. doi: 10.1016/j.ijbiomac.2019.12.242
[22]	GE S, YANG Y, CHELLIAPPAN B, et al. Evaluation of different IgY preparation methods and storage stability as potential animal feed supplement[J]. Pakistan Journal of Zoology,2020,52(6):2305−2311.
[23]	LEE H Y, ABEVRATHNE E D N S, CHOI I, et al. Sequential separation of immunoglobulin Y and phosvitin from chicken egg yolk without using organic solvents[J]. Poultry science,2014,93:2668−2677. doi: 10.3382/ps.2014-04093
[24]	XU C N, YIN Y G, WANG E L, et al. Optimized process of extracting phosvitin from hen egg yolk based on genetic algorithm[J]. Journal of Jilin University(Engineering and Technology Edition),2011,41(3):876−881.
[25]	LOSSO J, NAKAI S. A simple procedure for the isolation of phosvitin from chicken egg yolk[J]. Egg Uses and Processing Technologies, 1994, 21(2): 150−157.
[26]	CASTELLANIO, MARTINET V, DAVID-BRIAND E, et al. Egg yolk phosvitin: Preparation of metal-free purified protein by fast protein liquid chromatography using aqueous solvents[J]. Journal of Chromatography B,2003,791(1-2):273−284. doi: 10.1016/S1570-0232(03)00230-7
[27]	SAMOOEL J, UK A D, KI CHANG N, et al. Separation of phosvitin from egg yolk without using organic solvents[J]. Asian Australasian Journal of Animal Sciences,2013,26(11):1622−1629. doi: 10.5713/ajas.2013.13263
[28]	ZHANG X, QIU N, GENG F, et al. Simply and effectively preparing high-purity phosvitin using polyethylene glycol and anion-exchange chromatography[J]. Journal of Separation Science,2011,34(22):3295−3301. doi: 10.1002/jssc.201100601
[29]	KO K Y, NAM K C, JO C, et al. A simple and efficient method for preparing partially purified phosvitin from egg yolk using ethanol and salts[J]. Poultry Science,2011,90(5):1096−1104. doi: 10.3382/ps.2010-01138
[30]	SPRICK B, LINGHU Z, AMA MCH ARLA J K, et al. Selective extraction of phospholipids from whey protein phospholipid concentrate using supercritical carbon dioxide and ethanol as a co-solvent[J]. Journal of Dairy Science,2019,102(12):10855−10866. doi: 10.3168/jds.2019-16419
[31]	LI B, LINGHU Z, HUSSAIN F, et al. 0536 Extraction of phospholipids from procream using supercritical carbon dioxide and ethanol as a modifier[J]. Journal of Animal Science,2016,94(5):256.
[32]	LI Y, OBADI M, QI Y, et al. Extraction of oat lipids and phospholipids using subcritical propane and dimethyl ether: Experimental data and modeling[J]. European Journal of Lipid Science and Technology, 2020, 123(1).
[33]	BOSELLI E, CABONI M F. Supercritical carbon dioxide extraction of phospholipids from dried egg yolk without organic modifier[J]. Journal of Supercritical Fluids,2000,19(1):45−50. doi: 10.1016/S0896-8446(00)00073-5
[34]	周欣. 鸡卵黄免疫球蛋白Fab片段的制备及其抗炎活性研究[D]. 武汉: 华中农业大学, 2020. ZHOU X. Preparation of Fab fragment of immunoglobulin of egg yolk and its anti-inflammatory activity[D]. Wuhan: Huazhong Agricultural University, 2020.
[35]	MINE Y. Egg bioscience and biotechnology[J]. Egg Bioscience and Biotechnology-Research and Markets,2008,3(5):48−55.
[36]	刘颖, 肖静静, 李硕绵, 等. 蛋黄卵磷脂提取方法的研究[J]. 河北农业大学学报,2013,36(6):105−108. [LIU Y, XIAO J J, LI S J, et al. Study on the extraction methods of egg yolk lecithin[J]. Journal of Agricultural University of Hebei,2013,36(6):105−108.
[37]	REN J, WU J. Thermal-aided phosvitin extraction from egg yolk[J]. Journal of the Science of Food & Agriculture,2015,95:2595−2600.
[38]	JIANG B, WANG L, WANG X, et al. Ultrasonic thermal-assisted extraction of phosvitin from egg yolk and evaluation of its properties[J]. Polymers, 2019, 11(8).
[39]	NASOPOULOU C, GOGAKI V, PANAGOPOULOU E, et al. Hen egg yolk lipid fractions with antiatherogenic properties[J]. Animal Science Journal,2013,84(3):264−271.
[40]	NIELSEN H, SHUKLA V K S. In situ solid phase extraction of lipids from spray-dried egg yolk by ethanol with subsequent removal of triacylglycerols by cold temperature crystallization[J]. LWT - Food Science and Technology,2004,37(6):613−618. doi: 10.1016/j.lwt.2003.12.007
[41]	KOVALCUKS A, DUMA M. Solvent extraction of egg oil from liquid egg yolk[C]. Baltic Conference on Food Science & Technology 2014.
[42]	BELHAJ K, MANSOURI F, SINDIC M, et al. Effect of rearing season on meat and intramuscular fat quality of beni-guil sheep[J]. Journal of Food Quality,2021,101(7):1−9.
[43]	HOWES N L, BEKHIT E, BURRITT D J, et al. Opportunities and implications of pasture-based lamb fattening to enhance the long-chain fatty acid composition in meat[J]. Comprehensive Reviews in Food Science & Food Safety,2015,14(1):22−36.

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