The Stability of Krill Oil Emulsion Based on Interaction of Endogenous Phospholipids with Whey Protein Isolate

ZHAO Dianbo; QIN Xiaopeng; ZHAN Haijie; DUAN Ziqiang; YU Xiao; DENG Qianchun; XIANG Qisen; ZHU Yingying

doi:10.13386/j.issn1002-0306.2021040132

Volume 42 Issue 24

Dec. 2021

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Science and Technology of Food Industry > 2021 > 42(24): 68-76. > DOI: 10.13386/j.issn1002-0306.2021040132

ZHAO Dianbo, QIN Xiaopeng, ZHAN Haijie, et al. The Stability of Krill Oil Emulsion Based on Interaction of Endogenous Phospholipids with Whey Protein Isolate[J]. Science and Technology of Food Industry, 2021, 42(24): 68−76. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021040132.

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The Stability of Krill Oil Emulsion Based on Interaction of Endogenous Phospholipids with Whey Protein Isolate

1.
College of Food and Bioengineering, Zhengzhou University of Light Industry, Henan Key Laboratory of Cold Chain Food Quality and Safety Control, Collaborative Innovation Center for Food Production and Safety,Zhengzhou 450001, China
2.
Hubei Key Laboratory of Lipid Chemistry and Nutrition, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China

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Received Date: April 12, 2021
Available Online: October 13, 2021

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Abstract

Abstract

The construction of emulsion system with high physical stability is pivotal to effectively increase the bioavailability of krill oil and broaden its application in health food. This study aimed to investigate the effects and potential mechanism of the interaction between endogenous phospholipids and whey protein isolate(WPI) on the physicochemical properties, microstructure and physical stability of krill oil emulsion. The results showed that when the oil load increased to 25%, the average particle size and zeta potential value of krill oil emulsion reached 35.03 nm and −27.3 mV, respectively, emulsion tended to be more unstable. The addition of WPI 0.5% (w/v) increased the average particle size and Zeta potential value of krill oil emulsion by 84.0% and 31.40%(P<0.05), respectively. Meanwhile, the apparent viscosity values at low shear rate of krill oil emulsion increased by 7-folds(P<0.05), and the turbiscan stability index(TSI) value decreased by 70.3%(P<0.05), thereby leading to stable emulsion. Further results showed that endogenous phospholipids could interact with WPI to improve its interfacial activity, reduce α-helix content in WPI by 1.60%(P<0.05) and evidently enhance its endogenous fluorescence intensity and surface hydrophobicity. The environmental stress stability demonstrated that the simulated pasteurization heat treatment improved the physical stability of krill oil emulsion. In addition, the krill oil emulsion exhibited relatively strong stability when the pH values migrated toward weak alkaline(pH6~9). Therefore, the interaction of endogenous phospholipids and WPI would provide the possibility to construct krill oil emulsion with high physical stability and broadened its application in health food.
- krill oil emulsion,
- physical stability,
- interaction,
- simulated pasteurization,
- pH migration

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