ZHAO Huijuan, XU Tengteng, ZHAO Nan, et al. Composition and Activity Analysis of Supercritical CO2 Extract from Zanthoxylum bungeanum Residue[J]. Science and Technology of Food Industry, 2021, 42(24): 256−262. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021040083.
Citation: ZHAO Huijuan, XU Tengteng, ZHAO Nan, et al. Composition and Activity Analysis of Supercritical CO2 Extract from Zanthoxylum bungeanum Residue[J]. Science and Technology of Food Industry, 2021, 42(24): 256−262. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021040083.

Composition and Activity Analysis of Supercritical CO2 Extract from Zanthoxylum bungeanum Residue

More Information
  • Received Date: April 08, 2021
  • Available Online: October 14, 2021
  • To explore the constituents of Zanthoxylum bungeanum supercritical CO2 extract and its antibacterial and antioxidant effects. The compound composition was analyzed by high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS), its antibacterial activity was analyzed by paper diffusion and double gradient dilution. And the free radical scavenging rate of DPPH and ABTS+ of the extract was determined to denote antioxidant activity. The results showed that the extract consisted of alkaloids, enols, amides, flavonoids, coumarins and some other components. The relative content of dendromine was the highest, reaching 38.28%. Zanthoxylum bungeanum extract had a broad antimicrobial spectrum. The minimum inhibitory concentration (MIC) against Vibrio parahaemolyticus, Aeromonas hydrophila and Shewanella was of Zanthoxylum bungeanum residue extract were 3.125 mg/mL. Compared with Zanthoxylum bungeanum extract, zanthoxylin and Zanthoxylum bungeanum essential oil, Zanthoxylum bungeanum residue extract had the strongest antioxidant activity, with a clearance rate of 100% of ABTS+ free fundamentals at 12.5 mg/mL and 91.35% of DPPH free fundamentals at 50 mg/mL. Therefore, the Zanthoxylum bungeanum residue extract had better antibacterial and antioxidant properties. It had a broad development and application potential, could achieve the high-value utilization of Zanthoxylum bungeanum.
  • [1]
    杨清山, 翟彦伟, 栗星, 等. 花椒及其提取物中花椒麻素的HPLC测定方法[J]. 食品工业,2019,40(8):328−332. [YANG Q S, ZHAI Y W, LI X, et al. Determination of sanshool from Zanthoxylum and its extracts by HPLC[J]. The Food Industry,2019,40(8):328−332.
    [2]
    张艳军, 杨途熙, 魏安智, 等. 超临界CO2萃取花椒果皮总黄酮的工艺[J]. 食品工业科技,2013,34(5):188−191. [ZHANG Y J, YANG T X, WEI A Z, et al. Extraction of total flavonoids from Zanthoxylum pericarp by supercritical CO2[J]. Science and Technology of Food Industry,2013,34(5):188−191.
    [3]
    朱妞. 花椒活性成分研究现状及发展前景[J]. 粮食与油脂,2020,33(4):4−6. [ZHU N. Research status and development prospect of active components of Zanthoxylum[J]. Cereals and Oils,2020,33(4):4−6. doi: 10.3969/j.issn.1008-9578.2020.04.002
    [4]
    李玄. 花椒果皮黄酮多酚类物质提取及抗氧化抑菌活性研究[D]. 咸阳: 西北农林科技大学, 2018.

    LI X. Study on extraction and antioxidant and antibacterial activity of flavonoid polyphenols from Zanthoxylum pericarp[D]. Xianyang: North West Agriculture and Forestry University, 2018.
    [5]
    吴静. 花椒精油的提取工艺、化学成分分析与抗菌活性研究[D]. 合肥: 合肥工业大学, 2017.

    WU J. Extraction technology, chemical composition analysis and antibacterial activity of Zanthoxylum bungeanum essential oil[D]. Hefei: Hefei University of Technology, 2017.
    [6]
    周孟焦, 史芳芳, 陈凯, 等. 花椒药用价值研究进展[J]. 农产品加工,2020(1):65−67,72. [ZHOU M J, SHI F F, CHEN K, et al. Research progress on medicinal value of Zanthoxylum[J]. Academic Periodical of Farm Products Processing,2020(1):65−67,72.
    [7]
    PANG W W, LIU S, HE F T, et al. Anticancer activities of Zanthoxylum bungeanum seed oil on malignant melanoma[J]. Journal of Ethnopharmacology,2019,229:180−189. doi: 10.1016/j.jep.2018.10.012
    [8]
    WANG Z F, HE Z F, ZHANG D, et al. Effect of pepper (Zanthoxylum bungeanum Maxim.) essential oil on quality changes in rabbit meat patty during chilled storage[J]. Journal of Food Science and Technology, 2021: 1-11.
    [9]
    张慧, 杨东元, 孙林涛, 等. 超临界CO2法制备食用调味花椒油的工艺[J]. 粮食科技与经济,2009,34(4):51−52. [ZHANG H, YANG D Y, SUN L T, et al. Preparation of edible flavoring Zanthoxylum bungeanum oil by supercritical CO2[J]. Grain Science and Technology and Economy,2009,34(4):51−52. doi: 10.3969/j.issn.1007-1458.2009.04.021
    [10]
    刘琳琪, 赵晨曦, 李佩娟, 等. 花椒挥发油超临界CO2萃取的工艺优化及GC-MS分析[J]. 现代食品科技,2020,36(5):73−80. [LIU L Q, ZHAO C X, LI P J, et al. Optimization of supercritical CO2 extraction and GC-MS analysis of volatile oil from Zanthoxylum bungeanum[J]. Modern Food Science and Technology,2020,36(5):73−80.
    [11]
    薛世开, 朱东英, 黄思宇, 等. 超临界CO2萃取西藏黄玛卡挥发油工艺优化及GC-MS分析[J]. 精细化工中间体,2020,50(4):24−29. [XUE S K, ZHU D Y, HUANG S Y, et al. Process optimization and GC-MS analysis of volatile oil from Tibet huangmaka by supercritical CO2 extraction[J]. Fine Chemical Intermediates,2020,50(4):24−29.
    [12]
    CARLOTTA C, FRANCESCO C, EMANUELE A, et al. Supercritical CO2 extraction of natural antibacterials from low value weeds and agro-waste[J]. Journal of CO2 Utilization,2020,40:101198. doi: 10.1016/j.jcou.2020.101198
    [13]
    CHEN W, GONG L, GUO Z, et al. A novel integrated method for large-scale detection, identification, and quantification of widely targeted metabolites: Application in the study of rice metabolomics[J]. Molecular Plant,2013,6(6):1769−1780. doi: 10.1093/mp/sst080
    [14]
    干信, 吴士筠, 高雯琪. 花椒挥发油抑菌作用研究[J]. 食品科学,2009,30(21):128−130. [GAN X, WU S J, GAO W Q. Study on the antibacterial effect of essential oil of Zanthoxylum bungeanum[J]. Food Science,2009,30(21):128−130. doi: 10.3321/j.issn:1002-6630.2009.21.030
    [15]
    叶磊. 山楂核干馏油抑菌及杀菌作用的研究[J]. 北京联合大学学报(自然科学版),2003(3):48−51. [YE L. Study on bacteriostasis and bactericidal effect of hawthorn kernel retort oil[J]. Journal of Beijing Union University (Natural Science Edition),2003(3):48−51.
    [16]
    万敏, 姚于飞, 付王威, 等. 黄金茶挥发油提取工艺优化、成分分析及抗氧化研究[J/OL]. 食品工业科技, 2021. https://doi.org/10.13386/j.issn1002-0306.

    WAN M, YAO Y F, FU W W, et al. Optimization of extraction process, component analysis and antioxidant activity of essential oil from Camellia chrysantha[J/OL]. Science and Technology of Food Industry, 2021. https://doi.org/10.13386/j.issn1002-0306.
    [17]
    伍燕, 周中秋, 汪伟, 等. 两种花椒精油成分GC-MS分析及活性研究[J]. 广州化工,2020,48(24):111−114,137. [WU Y, ZHOU Z Q, WANG W, et al. GC-MS analysis and activity of essential oil of two Zanthoxylum bungeanum[J]. Guangzhou Chemical Industry,2020,48(24):111−114,137. doi: 10.3969/j.issn.1001-9677.2020.24.037
    [18]
    王荣, 罗倩, 冯怡. DPPH、ABTS和FRAP微量法测定山奈酚的抗氧化能力[J]. 广州化工,2021,49(3):58−59, 63. [WANG R, LUO Q, FENG Y. Determination of antioxidant capacity of kaempferol by DPPH, ABTS and FRAP[J]. Guangzhou Chemical Industry,2021,49(3):58−59, 63. doi: 10.3969/j.issn.1001-9677.2021.03.019
    [19]
    柴丽琴, 原洪, 王立霞, 等. 花椒油树脂酶法提取工艺优化及GC-MS分析[J]. 食品与机械,2017,33(9):162−166, 172. [CHAI L Q, YUAN H, WANG L X, et al. Optimization of enzymatic extraction and GC-MS analysis of Zanthoxylum bungeanum oleoresin[J]. Food and Machinery,2017,33(9):162−166, 172.
    [20]
    WANG Y, YANG S H, ZHONG K Y, et al. Network pharmacology-based strategy for the investigation of the anti-obesity effects of an ethanolic extract of Zanthoxylum bungeanum Maxim[J]. Frontiers in Pharmacology,2020:572387.
    [21]
    王珊珊, 刘佳萌, 孙晶, 等. 药用石斛属植物石斛碱研究进展[J]. 中国食物与营养,2020,26(12):9−15. [WANG S S, LIU J M, SUN J, et al. Research progress of dendrobine in medicinal Dendrobium[J]. Food and Nutrition in China,2020,26(12):9−15. doi: 10.3969/j.issn.1006-9577.2020.12.003
    [22]
    YANG Q Q, MEI X F, WANG Z R, et al. Comprehensive identification of non-volatile bitter-tasting compounds in Zanthoxylum bungeanum Maxim. by untargeted metabolomics combined with sensory-guided fractionation technique[J]. Food Chemistry,2021:129085.
    [23]
    HUSEYIN D, HATICE E. Relationship between volatile components, antimicrobial and antioxidant properties of the essential oil, hydrosol and extracts of Citrus aurantium L. flowers[J]. Journal of Infection and Public Health,2020,13(1):58−67. doi: 10.1016/j.jiph.2019.06.017
    [24]
    YU L, WU W L, PAN Y Y, et al. Quality evaluation of different varieties of Zanthoxylum bungeanum Maxim. peels based on phenolic profiles, bioactivity, and HPLC fingerprint[J]. Journal of Food Science,2020,85(4):1090−1097. doi: 10.1111/1750-3841.15095
    [25]
    赖燕娜. 石斛花的功效研究[J]. 科技风,2020(18):277. [LAI Y N. Study on the efficacy of Dendrobium flower[J]. Technology Wind,2020(18):277.
    [26]
    赵强, 徐未芳, 王桂林, 等. 花椒油树脂提取工艺及其生物活性研究进展[J]. 粮食与油脂,2021,34(1):4−6, 15. [ZHAO Q, XU W F, WANG G L, et al. Research progress on extraction technology and biological activity of Zanthoxylum bungeanum oleoresin[J]. Cereals and Oils,2021,34(1):4−6, 15. doi: 10.3969/j.issn.1008-9578.2021.01.002
  • Cited by

    Periodical cited type(3)

    1. 杨丽凤,毛书灿,汪兰,李平,周志,熊光权,石柳. 宰杀方式对鳝鱼肌肉品质的影响. 食品与机械. 2023(01): 132-138 .
    2. 张震,邱爽,袁娟,夏琳琳,刘春艳,吕晓华. 稳定态二氧化氯对微冻贮藏水产品的防腐保鲜效果研究. 现代预防医学. 2023(08): 1468-1475 .
    3. 范铭良,郝淑贤,李来好,陈胜军,岑剑伟,吴燕燕,魏涯,相欢,黄卉. 真空冷藏条件下罗非鱼内源蛋白酶对鱼片质构劣化的作用. 南方农业学报. 2023(02): 555-563 .

    Other cited types(2)

Catalog

    Article Metrics

    Article views (337) PDF downloads (31) Cited by(5)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return