Study on the Potential Mechanism of Synsepalum dulcificum Interfering with Tumor Immunity

Objective: To study the material basis and possible mechanism of the pharmacodynamics of Synsepalum dulcificum's intervention in tumor immunity. Methods: The study used liquid phase-mass spectrometry analysis to clarify the composition of Synsepalum dulcificum, and then used the network pharmacology and bioinformatics methods to systematically study the specific impact of Synsepalum dulcificum on tumor immunity. At the same time, it was clear that Synsepalum dulcificum medicine and food were homologous.Results: The liquid-mass spectrometry experiment revealed that Synsepalum dulcificum may contain 360 components. After comparing with the tumor immunity database, 42 components related to tumor immunity and 55 related targets were obtained. After screening, key targets in the target network were obtained. These were TSHR, TP53, MAPK1, HIF1A, CA9. Further collection of experimental information in the literature and molecular docking experiments verified that the different components of Synsepalum dulcificum could affect the selection of targets and interfere with tumor immunity. Conclusion: Through the above experiments, it is found that the components of Synsepalum dulcificum may interact with tumor immune-related targets, paving the way for the research on the direct effect of Synsepalum dulcificum on tumors.