Optimization of Extraction Process and Component Analysis of Oleoresin from Smoked <i>Amomum tsaoko</i> by Response Surface Methodology

LIN Yi; HUANG Mei; WU Guiping; XU Fei; HE Juncai; YOU Shengjun; GAO Penghui; LIU Linna; HOU Meifang; GU Fenglin

doi:10.13386/j.issn1002-0306.2021040002

Volume 43 Issue 1

Dec. 2021

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Science and Technology of Food Industry > 2022 > 43(1): 163-171. > DOI: 10.13386/j.issn1002-0306.2021040002

LIN Yi, HUANG Mei, WU Guiping, et al. Optimization of Extraction Process and Component Analysis of Oleoresin from Smoked Amomum tsaoko by Response Surface Methodology[J]. Science and Technology of Food Industry, 2022, 43(1): 163−171. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021040002.

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Optimization of Extraction Process and Component Analysis of Oleoresin from Smoked Amomum tsaoko by Response Surface Methodology

LIN Yi^{1, 2, 3,},
HUANG Mei^{2, 3, 4},
WU Guiping^{2, 3, ,},
XU Fei^{2, 3},
HE Juncai⁵,
YOU Shengjun⁵,
GAO Penghui⁶,
LIU Linna⁶,
HOU Meifang^1, ,,
GU Fenglin^{2, 3}

1.
School of Ecological Technology and Engineering, Shanghai Institute of Technology, Shanghai 201418, China
2.
Spice and Beverage Research Institute, Chinese Academy of Tropical Agriculture Science, Wanning 571533, China
3.
Key Laboratory of Processing Suitability and Quality Control of the Special Tropical Crops of Hainan Province, Wanning 571533, China
4.
School of Food and Wine, Ningxia University, Yinchuan 750021,China
5.
Nujiang Green Spice Industry Research Institute, Nujiang 673200, China
6.
Nujiang Prefecture Rural Science and Technology Productivity Promotion Center, Nujiang 673200, China

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Received Date: April 01, 2021
Available Online: November 05, 2021

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Abstract

Abstract

In order to obtain the best extraction process of smoked Amomum tsaoko(SDAT) oleoresin, the extraction solvent, extraction temperature, solid-liquid ratio, extraction time and particle size were regarded as the influence factors. On the basis of single factor experiment, the eexperimental design of three factors and three levels was used by Box-Benhnken central composite method, according to the extraction rate of SDAT oleoresin as the response value, and the extraction conditions were further optimized by response surface methodology(RSM). The results showed that the optimum process conditions of SDAT oleoresin was as follows: Anhydrous methanol as solvent, extraction temperature was 75 ℃, solid-liquidratio was 1:20(g/mL), extraction time was 7 h and particle size was 80 mesh. The regression equation predicted that the theoretical extraction rate of SDAT could reach 9.57%, the average extraction rate of the three validation experiments was 9.48%, and the predicted value was close to the actual result. And 58 compounds were identified by GC-TOF-MS, including alcohols(31.59%), aldehydes(21.13%), alkenes(1.23%), ketones(1.56%), esters(2.38%) and phenols(0.79%), 1,8-eucalyptol(9.28%), α-terpineol(3.55%), gera-niol(8.4%), trans citral(5.78%), 2-methyl-3-ph-enylpropanal(6.33%), etc. Through the determination of the best process of SDAT oleoresin, the detected components are mainly concentrated in alcohols and aldehydes, which provides a theoretical basis for the nutritional components and biological activity of grass fruit oleoresin in the later stage.
- smoked Amomum tsaoko,
- heating reflux method,
- response surface method,
- GC-TOF-MS,
- component analysis

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