Effect of Combined Oyster Peptide and Ginseng Extracts on Improvement of Sexual Function and Anti-fatigue in Male Mice

LUO Xianliang; YAN Yongqiu; FENG Fengqin

doi:10.13386/j.issn1002-0306.2021030384

Volume 43 Issue 1

Dec. 2021

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Science and Technology of Food Industry > 2022 > 43(1): 366-374. > DOI: 10.13386/j.issn1002-0306.2021030384

LUO Xianliang, YAN Yongqiu, FENG Fengqin. Effect of Combined Oyster Peptide and Ginseng Extracts on Improvement of Sexual Function and Anti-fatigue in Male Mice[J]. Science and Technology of Food Industry, 2022, 43(1): 366−374. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021030384.

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Effect of Combined Oyster Peptide and Ginseng Extracts on Improvement of Sexual Function and Anti-fatigue in Male Mice

1.
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China
2.
Ningbo Yufangtang Biotechnology Co., Ltd., Ningbo 315012, China

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Received Date: March 30, 2021
Available Online: November 07, 2021

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Abstract

Abstract

This study aimed to explore the effects of the combination of oyster peptide and ginseng extracts (OPGE) on exercise fatigue and sexual function in normal male mice. In this experiment, ICR male mice were randomly divided into 6 groups, including control group, oyster peptide (0.2 mg/g bw, OP), ginseng extract (0.2 mg/g bw, GE), low dosage of OPGE (0.1 mg/g bw, OPGEL), medium dosage of OPGE (0.2 mg/gbw, OPGEM) and high dosage of OPGE (0.6 mg/g bw, OPGEH). All animals were treated continuously by oral gavage for 42 days, and then the swimming experiment and mating test were respectively performed on the last day of administration. Anti-fatigue and sexual function-related biochemical parameters were measured. The mating ability of mice was significantly enhanced in each treatment group compared with control group. The mating ability of OP and GE treated alone showed inferior to OPGEM administration, but without statistical difference. Compared with the control group, the serum levels of testosterone and follicle-stimulating hormone, the nitric oxide level in penis was increased by 20.02%, 19.92% and 17.19%, respectively, while the content of phosphodiesterases-5 in penis was decreased in OPGEM group. The results indicated that OPGEM administration presented the best effect on the promotion of sexual function among all groups (P<0.05). Besides, the expressions of steroid hormone synthesis acute regulatory protein (StAR) and 17β-hydroxysteroid dehydrogenase (HSD17B3) in the testis were up-regulated by OPGE administration, but without a statistical difference compared with the control group (P>0.05). Furthermore, the levels of lactic acid (P<0.01), urea nitrogen (P<0.05) and malondialdehyde (P<0.01) were decreased by the administration of OPGEM, while the content of glycogen was increased (P<0.01). In conclusion, OPGE presented a synergistic effect on anti-fatigue and improvement of sexual ability in normal male mice. The results are beneficial to promote the development of the functional food industry in the related field.
- oyster peptide,
- ginseng extracts,
- anti-fatigue,
- sexual function,
- synergistic effect

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References

[1]	KAYODE O T, YAKUBU M T. Parquetina nigrescens leaves: Chemical profile and influence on the physical and biochemical indices of sexual activity of male Wistar rats[J]. Journal of Integrative Medicine,2017,15(1):64−76. doi: 10.1016/S2095-4964(17)60318-2
[2]	TANG W H, ZHUANG X J, SHU R M, et al. The prevalence of erectile dysfunction among subjects with late-onset hypogonadism: A population-based study in China[J]. International Journal of Clinical and Experimental Medicine,2015,8(8):13901−13910.
[3]	ZHANG X, YANG B, LI N, et al. Prevalence and risk factors for erectile dysfunction in Chinese adult males[J]. Journal of Sexual Medicine,2017,14(10):1201−1208. doi: 10.1016/j.jsxm.2017.08.009
[4]	井宏颖, 吕克宁, 宋晓晨, 等. 地黄饮子对运动性疲劳小鼠运动能力的影响[J]. 湖南中医药大学学报,2021,41(1):34−38. [JING H Y, LV K, SONG X C, et al. Effect of radix rehmanniae decoction on exercise ability in exercise-induced fatigue mice[J]. Journal of Hunan University of Chinese Medicine,2021,41(1):34−38. doi: 10.3969/j.issn.1674-070X.2021.01.007
[5]	TADAYON N, BORNA J, MORTEZA G, et al. Vitamin E and ginseng combined supplement for treatment of male erectile dysfunction: A double-blind, placebo-controlled, randomized, clinical trial[J]. Advances in Integrative Medicine,2021,8(1):44−49. doi: 10.1016/j.aimed.2019.12.001
[6]	谢柏艳, 李文博, 杨玉平. 液相色谱-串联质谱法测定抗疲劳类保健食品中非法添加的西地那非含量的不确定度评定[J]. 食品安全质量检测学报,2021,12(12):1113−1118. [XIE B Y, LI W B, YANG Y P. Uncertainty evaluation for the determination of sildenafil illegally added in anti-fatigue health food by liquid chromatography-tandem mass spectrometry[J]. Journal of Food Safety and Quality,2021,12(12):1113−1118.
[7]	YANG Q Y, LAI X D, OUYANG J, et al. Effects of Ginsenoside Rg3 on fatigue resistance and SIRT1 in aged rats[J]. Toxicology,2018,409:144−151. doi: 10.1016/j.tox.2018.08.010
[8]	MA N, TAO H, DU H, et al. Antifatigue effect of functional cookies fortified with mushroom powder (Tricholoma matsutake) in mice[J]. Journal of Food Science,2020,85(12):4389−4395. doi: 10.1111/1750-3841.15510
[9]	孟超, 肖凯文, 郑双艳, 等. 蜂王浆主蛋白对小鼠的抗疲劳作用[J]. 中国食品学报,2017,10(7):23−29. [MENG C, XIAO K W, ZHENG S Y, et al. Anti-fatigue activity of MRJPs from fresh royal jelly in mice[J]. Journal of Chinese Institute of Food Science and Technology,2017,10(7):23−29.
[10]	COQUEIRO A Y, ROGERO M M, TIRAPEGUI J. Glutamine as an anti-fatigue amino acid in sports nutrition[J]. Nutrients,2019,11(4):1−19.
[11]	ZHANG X, JING S, LIN H, et al. Anti-fatigue effect of anwulignan via the NRF2 and PGC-1alpha signaling pathway in mice[J]. Food & Function,2019,10(12):7755−7766.
[12]	李旭龙, 陈洪鑫. 运动疲劳对人体姿势控制功能影响的研究综述[J]. 湖北体育科技,2017,36(11):971−974. [LI X L, CHEN H X. Influence of exercise fatigue on postural control: A review of literature[J]. Hubei Sports Science,2017,36(11):971−974. doi: 10.3969/j.issn.1003-983X.2017.11.009
[13]	XI X, GUO S, GUO H, et al. Anti-exercise-fatigue and promotion of sexual interest activity of total flavonoids from wasps drone-pupae in male mice[J]. Biomedicine & Pharmacotherapy,2018,107:254−261.
[14]	LUO C, XU X, WEI X, et al. Natural medicines for the treatment of fatigue: Bioactive components, pharmacology, and mechanisms[J]. Pharmacological Research,2019,148:104409. doi: 10.1016/j.phrs.2019.104409
[15]	CUI J, XIA P, ZHANG L, et al. A novel fermented soybean, inoculated with selected Bacillus, Lactobacillus and Hansenula strains, showed strong antioxidant and anti-fatigue potential activity[J]. Food Chemistry,2020,333:e127527. doi: 10.1016/j.foodchem.2020.127527
[16]	张亭, 李迪, 乌兰, 等. 牡蛎低聚肽配伍核桃低聚肽和山药多糖对雄性小鼠性功能的影响[J]. 现代预防医学,2018,45(12):2141−2153. [ZHANG T, LI D, WU L, et al. Effect of compatibilities of oyster oligopeptides, walnut oligopeptides and yam polysaccharide on sexual function in male mice[J]. Modern Preventive Medicine,2018,45(12):2141−2153.
[17]	ZHANG Z, SU G, ZHOU F, et al. Alcalase-hydrolyzed oyster (Crassostrea rivularis) meat enhances antioxidant and aphrodisiac activities in normal male mice[J]. Food Research International,2019,120:178−187. doi: 10.1016/j.foodres.2019.02.033
[18]	RIDZWAN B H, HANANI M, SITI NORSHUHADAA M P, et al. Screening for aphrodisiac property in local oyster of Crassostrea iredalei[J]. World Applied Sciences Journal,2013,26(12):1546−1551.
[19]	刘瑜, 张海欣, 盛卓娴, 等. 牡蛎低聚肽对CTX诱导的PADAM大鼠性功能及生殖功能的干预作用[J]. 食品工业科技,2020,41(17):302−307. [LIU Y, ZHANG H X, SHENG Z X, et al. Intervention of oyster oligopeptides on sexual and reproductive functions in PADAM rats induced by CTX[J]. Science and Technology of Food Industry,2020,41(17):302−307.
[20]	尹佳乐, 陈越, 张海悦. 蒲公英人参复合保健饮料研制及其抗疲劳功能研究[J]. 食品科技,2020,45(11):82−89. [YIN J L, CHEN Y, ZHANG H Y. Study on compound health beverage of dandelion ginseng and its anti-fatigue function[J]. Food Science and Technology,2020,45(11):82−89.
[21]	WANG C, LIU J, DENG J, et al. Advances in the chemistry, pharmacological diversity, and metabolism of 20(R)-ginseng saponins[J]. Journal of Ginseng Research,2020,44(1):14−23. doi: 10.1016/j.jgr.2019.01.005
[22]	鲍雷, 王军波, 张远, 等. 吉林人参低聚肽对雄性小鼠性功能影响的实验研究[J]. 中国预防医学杂志,2015,16(10):757−760. [BAO L, WANG J B, ZHANG Y, et al. Effects of Panax ginseng oligopeptide of Jilin on sexual function in male mice[J]. China Prevention medicine,2015,16(10):757−760.
[23]	SATTAYASAI J, SRISUWAN S, ARKARAVICHIEN T, et al. Effects of andrographolide on sexual functions, vascular reactivity and serum testosterone level in rodents[J]. Food Chemistry Toxicology,2010,48(7):1934−8. doi: 10.1016/j.fct.2010.04.037
[24]	LI Z Q, YU Y G, LI Y, et al. Taxifolin attenuates the developmental testicular toxicity induced by di-n-butyl phthalate in fetal male rats[J]. Food Chemistry Toxicology,2020,142:111482. doi: 10.1016/j.fct.2020.111482
[25]	中华人民共和国卫生部. 保健食品检验与评价技术规范[S]. 2003: 87. Ministry of Health of the People’s Republic of China. Technical specifications for health food inspection and evaluation[S]. 2003: 87.
[26]	CHINDE S, GROVER P. Toxicological assessment of nano and micron-sized tungsten oxide after 28 days repeated oral administration to Wistar rats[J]. Mutat Res Gen Tox En,2017,819:1−13. doi: 10.1016/j.mrgentox.2017.05.003
[27]	周兴, 李波男, 周海亮, 等. 雄蚕益肾方对LOH大鼠Leydig细胞胆固醇转运蛋白、睾酮合成酶和SF-1表达的影响[J]. 中华男科学杂志,2020,26(3):258−264. [ZHOU X, LI B N, ZHOU H L, et al. Xiongcan Yishen prescription up-regulates the expression of cholesterol transport proteins, steroidogenic enzymes and SF-1 in the Leydig cells of rats with late-onset hypogonadism[J]. National Journal of Andrology,2020,26(3):258−264.
[28]	REBOURCET D, MACKAY R, DARBEY A, et al. Ablation of the canonical testosterone production pathway via knockout of the steroidogenic enzyme HSD17B3, reveals a novel mechanism of testicular testosterone production[J]. The FASEB Journal,2020,34(8):10373−10386. doi: 10.1096/fj.202000361R
[29]	LI Z M, LIU N, JIANG Y P, et al. Vitexin alleviates streptozotocin-induced sexual dysfunction and fertility impairments in male mice via modulating the hypothalamus-pituitary-gonadal axis[J]. Chemico-Biological Interactions,2019,297:119−129. doi: 10.1016/j.cbi.2018.10.013
[30]	张姹, 魏易焓, 卢静怡, 等. 不同运动方式和运动时间对小鼠睾酮分泌和精子生成的影响[J]. 中国畜牧杂志,2021,57(2):96−100. [ZHANG C, WEI Y H, LU J Y. Effects of different exercise modes and exercise time on testosterone secretion and sperm production in mice[J]. Chinese Journal of Animal Science,2021,57(2):96−100.
[31]	NAM S Y, KIM H M, JEONG H J. Anti-fatigue effect by active dipeptides of fermented porcine placenta through inhibiting the inflammatory and oxidative reactions[J]. Biomedicine Pharmacother,2016,84:51−59. doi: 10.1016/j.biopha.2016.09.012
[32]	SGRÒ, PAOLO. Exercise, training, and the hypothalamic-pituitary-gonadal axis in men[J]. Current Opinion in Endocrine and Metabolic Research,2019,9:86−89. doi: 10.1016/j.coemr.2019.08.004
[33]	LIM P H C. Asian herbals and aphrodisiacs used for managing ED[J]. Translation Andrology Urology,2017,6(2):167−175. doi: 10.21037/tau.2017.04.04
[34]	CHEN J B, LI M J, CHEN L X, et al. Effects of Raphani Semen on anti-fatigue and pharmacokinetics of Panax ginseng[J]. Chinese Herbal Medicines,2019,11(3):308−313. doi: 10.1016/j.chmed.2019.05.001
[35]	GAO Y, ZHU P, XU S F, et al. Ginsenoside Re inhibits PDGF-BB-induced VSMC proliferation via the eNOS/NO/cGMP pathway[J]. Biomedicine & Pharmacotherapy,2019,115:108934.
[36]	ADEBAYO A A, OBOH G, ADEMOSUN A O. Almond-supplemented diet improves sexual functions beyond Phosphodiesterase-5 inhibition in diabetic male rats[J]. Heliyon,2019,5(12):e03035. doi: 10.1016/j.heliyon.2019.e03035
[37]	ZHANG Y Y, RYU B, CUI Y H, et al. A peptide isolated from Hippocampus abdominalis improves exercise performance and exerts anti-fatigue effects via AMPK/PGC-1α pathway in mice[J]. Journal of Functional Foods,2019,61:103489. doi: 10.1016/j.jff.2019.103489
[38]	陶雅浩, 金其贯, 徐昊然. 牡蛎肽补充和运动训练对小鼠运动耐力的影响[J]. 食品科技,2020,45(3):57−63. [TAO Y H, JIN Q G, XU H R. Effects of oyster peptide supplementation and exercise training on exercise endurance in mice[J]. Food Science and Technology,2020,45(3):57−63.
[39]	HU M, DU J, DU L D, et al. Anti-fatigue activity of purified anthocyanins prepared from purple passion fruit (P. edulis Sim) epicarp in mice[J]. Journal of Functional Foods,2020,65:103725. doi: 10.1016/j.jff.2019.103725
[40]	WANG P X, ZENG H L, LIN S L, et al. Anti-fatigue activities of hairtail (Trichiurus lepturus) hydrolysate in an endurance swimming mice model[J]. Journal of Functional Foods,2020,74:104207. doi: 10.1016/j.jff.2020.104207