Adsorption Mechanism and Process Optimization of <i>Inonotus obliquus</i> Polysaccharide Pigment by Macroporous Resin

XIN Chuanxin; YANG Jintao; DAI Xiaojing; SU Ling; WANG Qi

doi:10.13386/j.issn1002-0306.2021030304

Volume 42 Issue 22

Nov. 2021

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Science and Technology of Food Industry > 2021 > 42(22): 193-201. > DOI: 10.13386/j.issn1002-0306.2021030304

XIN Chuanxin, YANG Jintao, DAI Xiaojing, et al. Adsorption Mechanism and Process Optimization of Inonotus obliquus Polysaccharide Pigment by Macroporous Resin[J]. Science and Technology of Food Industry, 2021, 42(22): 193−201. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021030304.

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Adsorption Mechanism and Process Optimization of Inonotus obliquus Polysaccharide Pigment by Macroporous Resin

XIN Chuanxin^{1, 2,},
YANG Jintao^{1, 3},
DAI Xiaojing^{1, 2},
SU Ling^{1, 2, ,},
WANG Qi^{1, 2, ,}

1.
Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China
2.
College of Plant Protection, Jilin Agricultural University, Changchun 130118, China
3.
College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China

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Received Date: March 24, 2021
Available Online: September 06, 2021

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Abstract

Abstract

To explore the kinetic, thermodynamic characteristics and optimal conditions of the removal the inner pigment of the Inonotus obliquus polysaccharide by macroporous resin. In this study, the total Inonotus obliquus polysaccharide was prepared by water extraction and alcohol precipitation, Sevag method for protein removal, and dialysis. A preliminary screening of 17 macroporous resins were carried out by static adsorption method. And the score was calculated by pigment removal rate and polysaccharide retention rate. The parameters affecting the pigment inner Inonotus obliquus polysaccharide removal process, such as flow rate, adsorption time, ratio of diameter to height and sample concentration were studied and optimized along with evaluation of kinetic and thermodynamic parameters. The results showed that HPD-500 was the best suitable one among the 17 kinds of macroporous resins. The adsorption process followed pseudo-second-order model, which was affected by both intra particle diffusion and liquid film diffusion. The thermodynamic results showed that the adsorption was more in line with the Freundlich model, which was a multi-molecular layer adsorption. The rmodynamic parameters were also evaluated, enthalpy>0, Gibbs free energy<0, entropy>0, which showed that extraction was an endothermic, spontaneous and entropy increase reaction. Under the optimal conditions for removed pigment with elution ratio of diameter to height of 1:10, adsorption time of 1h, sample weight of 10 mg and flow rate of 1.5 mL/min, the pigment removal rate of Inonotus obliquus polysaccharide was 83.15%, the retention rate was 78.89%, with the purity promoted from 20.40% to 56.52%. The HPD-500 macroporous resin had the ability to remove the pigment and improve the purity of Inonotus obliquus polysaccharide. This article provided a theoretical and experimental basis for the efficient utilization of Inonotus obliquus resources.
- Inonotus obliquus polysaccharide,
- macroporous resin,
- pigment,
- kinetic,
- thermodynamic

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References (33)

References

[1]	李玉. 中国大型菌物资源图鉴[M]. 北京: 中国农业出版社, 2015: 474. LI Y. Atlas of Chinese macrofungai resources[M]. Beijing: China Agriculture Press, 2015: 474.
[2]	陈艳秋, 李玉. 桦褐孔菌的研究进展[J]. 微生物学通报,2005,32(2):124−127. [CHEN Y Q, LI Y. Development of the study in Inonotus obliquus[J]. Microbiology China,2005,32(2):124−127. doi: 10.3969/j.issn.0253-2654.2005.02.026
[3]	PARK Y M, WON J H, KIM Y H, et al. In vivo and in vitro anti-inflammatory and anti-nociceptive effects of the methanol extract of Inonotus obliquus[J]. Journal of Ethnopharmacol,2005,101:120−128. doi: 10.1016/j.jep.2005.04.003
[4]	SU B H, YAN X Z, LI Y Z, et al. Effects of Inonotus obliquus polysaccharides on proliferation, invasion, migration, and apoptosis of osteosarcoma cells[J]. Analytical Cellular Pathology (Amsterdam),2020,17(2020):4282036.
[5]	JIANG S P, SHI F L, LIN H, et al. Inonotus obliquus polysaccharides induces apoptosis of lung cancer cells and alters energy metabolism via the LKB1/AMPK axis[J]. International Journal of Biological Macromolecules,2019,151:1277−1286.
[6]	HAN Y Q, NAN S J, FAN J, et al. Inonotus obliquus polysaccharides protect against Alzheimer’s disease by regulating Nrf2 signaling and exerting antioxidative and antiapoptotic effects[J]. International Journal of Biological Macromolecules,2019,131:769−778. doi: 10.1016/j.ijbiomac.2019.03.033
[7]	XU X Q, LI J, HU Y. Polysaccharides from Inonotus obliquus sclerotia and cultured mycelia stimulate cytokine production of human peripheral blood mononuclear cells in vitro and their chemical characterization[J]. International Immunopharmacology,2014,21:269−278. doi: 10.1016/j.intimp.2014.05.015
[8]	YONG O K, SANG B H, HONG W L, et al. Immuno-stimulating effect of the endo-polysaccharide produced by submerged culture of Inonotus obliquus[J]. Life Sciences,2005,77(19):2438−2456. doi: 10.1016/j.lfs.2005.02.023
[9]	王耀晖, 马长治, 郭振宇, 等. 大孔树脂吸附梨果实中棒曲霉素的动力学模型研究[J]. 食品安全质量检测学报,2020,11(2):138−144. [WANG Y H, MA C Z, GUO Z Y, et al. Study on the kineticmodel of adsorption patulin in pear juice by macroporous resin[J]. Journal of Food Safety & Quality,2020,11(2):138−144.
[10]	黄明飞, 仝涛, 黄昆仑. 桦褐孔菌多糖的提取工艺、结构及功能活性研究进展[J]. 中国食用菌,2020,39(11):1−8, 13. [HUANG M F, TONG T, HHUANG K L. Research progress on extraction process, structure and functional activity of polysaccharides from Inonotus obliquus[J]. Edible Fungi of China,2020,39(11):1−8, 13.
[11]	刘伟, 刘倩楠, 张良, 等. 草莓多糖树脂法脱色及降血糖活性研究[J/OL]. 食品工业科技: 1−14[2021-02-24]. http://kns.cnki.net/kcms/detail/11.1759.TS.20191207.1208.032.html. LIU W, LIU Q N, ZHANG L, et al. Study on decoloration by macroporous resins and hypoglycemic activities of polysaccharides from strawberry[J/OL]. Science and Technology of Food Industry: 1−14[2021-02-24]. http://kns.cnki.net/kcms/detail/11.1759.TS.20191207.1208.032.html.
[12]	徐华容, 李丽琪, 刘阳, 等. AB-8大孔树脂纯化紫色马铃薯花色苷的工艺研究[J]. 中国药学杂志,2017,52(24):2141−2145. [XU H R, LI L Q, LIU Y, et al. Purification study of purple Solanum tuberosum anthocyanins by AB-8 macroporous[J]. Chinese Pharmaceutical Journal,2017,52(24):2141−2145.
[13]	陈卓尔, 李敏, 王小静, 等. 大孔吸附树脂对维药恰麻古粗多糖脱色纯化工艺研究[J]. 应用化工,2017,46(2):230−233, 239. [CHEN Z E, LI M, WANG X J, et al. Study on decolorization process of macroporous adsorption resin of crude polysaccharides from Xinjiang Brassica rapa L[J]. Applied Chemical Industry,2017,46(2):230−233, 239.
[14]	张清安, 范学辉, 张志琪. D301树脂对沙苑子酚类物质的吸附动力学研究[J]. 现代食品科技,2013,29(7):1471−1476. [ZHANG Q G, FAN X H, ZHANG Z Q. Adsorption properties of D301 resins on phenols in methanol extracts of Astragalus complanatus R. Br[J]. Modern Food Science and Technology,2013,29(7):1471−1476.
[15]	SHI Y Y, LIU T T, HAN Y, et al. An efﬁcient method for decoloration of polysaccharides from the sprouts of Toona sinensis (A. Juss.) Roem by anion exchange macroporous resins[J]. Food Chemistry,2017,217(15):461−468.
[16]	孙伟, 叶润, 蔡静, 等. 桑叶多糖的分离纯化及其抑菌活性研究[J]. 食品研究与开发,2021,42(4):149−155. [SUN W, YE R, CAI J, et al. Purification and antibacterial activity of polysaccharide from mulberry leaves[J]. Food Research and Development,2021,42(4):149−155.
[17]	李欣欣, 李文香. 桦褐孔菌多糖的分离纯化及其抗氧化活性测定[J/OL]. 食品工业科技: 1−13[2021-03-22]. https://doi.org/10.13386/j.issn1002-0306.2020040211. LI X X, LI W X. Isolation, purification and antioxidant activity of Inonotus obliquus polysaccharide[J/OL]. Science and Technology of Food Industry: 1−13[2021-03-22]. https://doi.org/10.13386/j.issn1002-0306.2020040211.
[18]	陈文灵. 采用D101树脂制备桦褐孔菌粗多糖的方法: 中国, 104045728[P]. 2014-06-25. CHEN W L. Method for preparing Inonotus obliquus crude polysaccharide by using D101 resin: China, 104045728[P]. 2014-06-25.
[19]	ZHANG L H, ZHAO P, MEN Q H, et al. Decolorization of Limonium bicolor Kunze (Bge.) polysaccharides by resins[J]. Advanced Materials Research,2014,978:48−51. doi: 10.4028/www.scientific.net/AMR.978.48
[20]	MEGHA S, NIHARIKA S V, REENA S. Highly efficient and rapid removal of a toxic dye: Adsorption kinetics, isotherm, and mechanism studies on functionalized multiwalled carbon nanotubes[J]. Surfaces and Interfaces,2020,21:100639. doi: 10.1016/j.surfin.2020.100639
[21]	刘富岗, 冯素香, 张杰, 等. 白屈菜多糖纯化工艺的优化[J]. 中成药,2018,40(2):475−477. [LIU F G, FENG S X, ZHANG J, et al. Optimization of the purification process of Chelidonium majus polysaccharide[J]. Chinese Traditional Patent Medicine,2018,40(2):475−477. doi: 10.3969/j.issn.1001-1528.2018.02.048
[22]	ANSHUMAN S, MINU S, KAJAL K, et al. Effective elimination of endocrine disrupting bisphenol A and S from drinking water using phenolic resin-based activated carbon fiber: Adsorption, thermodynamic and kinetic studies[J]. Environmental Nanotechnology Monitoring & Management,2020,14:100316.
[23]	欧阳玉祝, 张辞海, 魏燕. 大孔树脂对倍花单宁酸的吸附特性及其动力学模型[J]. 食品科学,2013,34(11):122−125. [OUYANG Y Z, ZHANG C H, WEI Y. A kinetic model for adsorption characteristics of tannic acid from Chinese gall “Beihua” on macroporous resin[J]. Food Science,2013,34(11):122−125. doi: 10.7506/spkx1002-6630-201311027
[24]	WANG J, LI A, XU L, et al. Adsorption of tannic and gallic acids on a new polymeric adsorbent and the effect of Cu(II) on their removal[J]. Journal of Hazardous Materials,2009,169(1/2/3):794−800.
[25]	GAO Z P, YU Z F, YUE T L, et al. Adsorption isotherm, thermodynamics and kinetics studies of polyphenols separation from kiwifruit juice using ad-sorbent resin[J]. Journal of Food Engineering,2013,116(1):195−201. doi: 10.1016/j.jfoodeng.2012.10.037
[26]	ESFANDIARI N, KASHEFI M, MIRJALILI M, et al. Role of silica mid-layer in thermal and chemical stability of hierarchical Fe₃O₄-SiO₂-TiO₂ nanoparticles for improvement of lead adsorption: Kinetics, thermodynamic and deep XPS investigation[J]. Materials Science and Engineering: B,2020,262:114690. doi: 10.1016/j.mseb.2020.114690
[27]	ANIRUDHAN T S, RADHAKRISHNAN P G. Kinetics, thermodynamics and surface heterogeneity assessment of uranium (VI) adsorption onto cation exchange resin derived from a lignocellulosic residue[J]. Applied Surface Science,2009,255(9):4983−4991. doi: 10.1016/j.apsusc.2008.12.047
[28]	何则强, 朱满香, 谢彪, 等. 大孔吸附树脂对湖南黑茶茶多酚的吸附热力学和动力学研究[J]. 离子交换与吸附,2020,36(2):117−124. [HE Z Q, ZHU M X, XIE B, et al. Study on kinetics and thermodynamics of tea polyphenols from hunan dark tea on macroporous adsorption resins[J]. Ion Exchange and Adsorption,2020,36(2):117−124.
[29]	范春雪, 何晨阳, 宗晨冉, 等. 大孔吸附树脂对淫羊藿苷的吸附热力学和动力学研究[J]. 离子交换与吸附,2020,36(3):221−230. [FAN C X, HE C Y, ZONG C R, et al. Study on adsorption thermodynamics and kinetics of icariin adsorbed on macroporous adsorption resins[J]. Ion Exchange and Adsorption,2020,36(3):221−230.
[30]	张斌, 伍亚华, 孙兰萍. 大孔树脂对花生壳总黄酮的吸附热力学研究[J]. 中国油脂,2018,43(7):36−40. [ZHANG B, WU Y H, SUN L P. Adsorption thermodynamics of total flavonoids from peanut hull by macroporous resin[J]. China Oils and Fats,2018,43(7):36−40. doi: 10.3969/j.issn.1003-7969.2018.07.010
[31]	陆敏, 马海乐, 朱莉萍, 等. D301-G大孔树脂吸附菊芋多糖色素机理探究[J]. 食品工业科技,2019,40(16):58−63, 70. [LU M, MA H L, ZHU L P, et al. Adsorption mechanism research on Jerusalem artichoke polysaccharide pigment with D301-G macroporous resin[J]. Science and Technology of Food Industry,2019,40(16):58−63, 70.
[32]	董施彬, 宁亚萍, 杨喆, 等. 藜麦总黄酮提取及大孔树脂纯化工艺的研究[J]. 食品工业科技,2015,36(16):272−278. [DONG S B, NING Y P, YANG Z, et al. Study on extraction process of quinoa total flavonoids and purification process of macroporous resin[J]. Science and Technology of Food Industry,2015,36(16):272−278.
[33]	玄光善, 郑晓, 孙钦勇, 等. 桦褐孔菌多糖的纯化及其单糖组成分析[J]. 中南药学,2014,12(10):981−984. [XUAN G S, ZHENG X, SUN Q Y, et al. Purification and monosaccharide composition of polysaccharides from Inonotus[J]. Central South Pharmacy,2014,12(10):981−984. doi: 10.7539/j.issn.1672-2981.2014.10.011

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