Evaluation of Uncertainty in Determination of Ethyl Carbamate Residues in Chinese Baijiu by UPLC-Q/Orbitrap HRMS

YANG Junlin; CHENG Pingyan; JIANG Lili; FENG Xiaobing; XIONG Xiaotong; YIN Yanyan; HU Feng; WANG Diqiang; ZHONG Fangda

doi:10.13386/j.issn1002-0306.2021030281

Volume 42 Issue 24

Dec. 2021

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Science and Technology of Food Industry > 2021 > 42(24): 239-246. > DOI: 10.13386/j.issn1002-0306.2021030281

YANG Junlin, CHENG Pingyan, JIANG Lili, et al. Evaluation of Uncertainty in Determination of Ethyl Carbamate Residues in Chinese Baijiu by UPLC-Q/Orbitrap HRMS[J]. Science and Technology of Food Industry, 2021, 42(24): 239−246. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021030281.

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Evaluation of Uncertainty in Determination of Ethyl Carbamate Residues in Chinese Baijiu by UPLC-Q/Orbitrap HRMS

Guizhou Maotai Distillery (Group) Xijiu Co., Ltd., Zunyi 564622, China

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Received Date: March 22, 2021
Available Online: October 19, 2021

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Abstract

Abstract

In order to improve the reliability of test results, the study aimed at evaluation of the measurement uncertainty in the determination of ethyl carbamate residues in Chinese Baijiu by UPLC-Q/Orbitrap HRMS. Based on evaluation and expression of uncertainty of measurement (GB/T 27418-2017), a mathematical model of uncertainty evaluation was established, and the sources of uncertainty were comprehensively analyzed, further each component was quantified and synthesized. The results showed that the main sources of uncertainty arose from the determination of the experimental accuracy/bias and its precision, the preparation of standard solution and curve fitting, and followed by other sources of uncertainty was relatively low. Furthermore, it was worth through our research when the expanded uncertainty was 8.00 μg/L for ethyl carbamate contents of 71.54 μg/L in Chinese Baijiu (k=2).
- Chinese baijiu,
- ethyl carbamate,
- uncertainty,
- the expanded uncertainty,
- UPLC-Q/Orbitrap HRMS

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References

[1]	LACHENMEIER D W, LIMA M CP, NÓBREGA I C C, et al. Cancer risk assessment of ethyl carbamate in alcoholic beverages from Brazil with special consideration to the spirits cachaça and tiquira[J]. BMC Cancer,2010,10(1):266. doi: 10.1186/1471-2407-10-266
[2]	GOWD V, SU H, KARLOVSKY P, et al. Ethyl carbamate: An emerging food and environmental toxicant[J]. Food Chemistry,2018,248:312−321. doi: 10.1016/j.foodchem.2017.12.072
[3]	刘爱东, 周萍萍, 刘飒娜, 等. 中国居民白酒中氨基甲酸乙酯健康风险概率评估[J]. 中国食品卫生杂志,2016,28(2):244−248. [LIU A D, ZHOU P P, LIU S N, et al. Probabilistic risk assessment of ethyl carbamate in spirits among Chinese population[J]. Chinese Journal of Food Hygiene,2016,28(2):244−248.
[4]	CARLSON G P. The effect of inducers and inhibitors of urethane metabolism on its in vitro and in vivo metabolism in rats[J]. Cancer Letters,1994,87(2):145−150. doi: 10.1016/0304-3835(94)90215-1
[5]	WEBER J V, SHARYPOV V I. Ethyl carbamate in foods and beverages: A review[J]. Environmental Chemistry Letters,2009,7(3):233−247. doi: 10.1007/s10311-008-0168-8
[6]	CHEN D W, REN Y P, ZHONG Q D, et al. Ethyl carbamate in alcoholic beverages from China: Levels, dietary intake, and risk assessment[J]. Food Control,2017,72:283−288. doi: 10.1016/j.foodcont.2015.10.047
[7]	林宜锦, 欧梦莹, 关统伟, 等. 酿造酒中氨基甲酸乙酯的研究进展[J]. 食品工业科技,2019,40(11):358−364. [LIN Y J, OU M Y, GUAN T W, et al. Research progress of ethyl carbamate in brewing wine[J]. Science and Technology of Food Industry,2019,40(11):358−364.
[8]	刘洋, 李运奎, 韩富亮, 等. 葡萄酒中氨基甲酸乙酯的研究进展[J]. 食品科学,2019,40(7):289−295. [LIU Y, LI Y K, HAN F L, et al. Ethyl carbamate in wine: A review[J]. Food Science,2019,40(7):289−295. doi: 10.7506/spkx1002-6630-20180211-131
[9]	舒志钢, 褚国良, 安康. 饮料酒中氨基甲酸乙酯检测方法研究进展[J]. 食品安全质量检测学报,2019,10(6):1594−1600. [SHU Z G, CHU G L, AN K. Development of analysis methods of ethyl carbamate in alcoholic beverages[J]. Journal of Food Safety & Quality,2019,10(6):1594−1600. doi: 10.3969/j.issn.2095-0381.2019.06.030
[10]	李小霞, 石潇璇, 周丹, 等. 气质联用仪测定白酒中氨基甲酸乙酯的不确定度的评定[J]. 酿酒,2018,45(2):111−115. [LI X X, SHI X X, ZHOU D, et al. The evaluation of the uncertainty in the determination of ethyl carbamate in liquor by GC-MS[J]. Liquor Making,2018,45(2):111−115. doi: 10.3969/j.issn.1002-8110.2018.02.034
[11]	ZHAO X R, JIANG C X. Determination of ethyl carbamate in fermented liquids by ultra high performance liquid chromatography coupled with a Q Exactive hybrid quadrupole-orbitrap mass spectrometer[J]. Food Chemistry,2015,177:66−71. doi: 10.1016/j.foodchem.2015.01.025
[12]	PHILIPPUS A, MARIA A S, ANDRÉ D V. Development of a novel solid-phase extraction, LC-MS/MS method for the analysis of ethyl carbamate in alcoholic beverages: Application to South African wine and spirits[J]. Food Additives & Contaminants: Part A,2011,28(7):826−839.
[13]	王丽娟, 柯润辉, 王冰, 等. 超高效液相色谱-电喷雾串联质谱法直接测定黄酒和葡萄酒中氨基甲酸乙酯[J]. 色谱,2012,30(9):903−907. [WANG L J, KE R H, WANG B, et al. Direct determination of ethyl carbamate in Chinese rice wine and grape wine by ultra performance liquid chromatography electrospray ionization tandem mass spectrometry[J]. Chinese Journal of Chromatography,2012,30(9):903−907.
[14]	柯润辉, 王丽娟, 安红梅, 等. 超高效液相色谱-串联质谱法同时测定白酒中氨基甲酸乙酯和8种甜味剂[J]. 食品与发酵工业,2016,42(4):174−178. [KE R H, WANG L J, AN H M, et al. Simultaneous determination of ethyl carbamate and eight sweeteners in Chinese distilled spirit by ultra performance liquid chromatography-tandem mass spectrometry[J]. Food and Fermentation Industries,2016,42(4):174−178.
[15]	黄秋婷, 王成龙, 王宇, 等. 基于QuEChERS-四级杆/静电场轨道阱高分辨质谱法测定酱油类调味品中的氨基甲酸乙酯[J]. 中国调味品,2019,44(6):168−171,176. [HUANG Q T, WANG C L, WANG Y, et al. Determination of ethyl carbamate in soy sauce seasonings by quadrupole/electrostatic field qrbitraphigh-resolution mass spectrometry[J]. China Condiment,2019,44(6):168−171,176. doi: 10.3969/j.issn.1000-9973.2019.06.039
[16]	熊晓通, 胡峰, 尤小龙, 等. 超高效液相色谱-高分辨质谱测定白酒中氨基甲酸乙酯含量[J]. 食品科学,2021,42(8):283−287. [XIONG X T, HU F, YOU X L, et al. Determination and quantitation of ethyl carbamate in liquor by UPLC-high resolution mass spectrometry[J]. Food Science,2021,42(8):283−287. doi: 10.7506/spkx1002-6630-20191030-333
[17]	翟洪稳, 范素芳, 王娟, 等. 测量不确定度在食品检验中的应用及进展[J]. 食品科学,2021,42(5):314−320. [ZHAI H W, FANG S F, WANG J, et al. Application and progress of measurement uncertainty in food inspection and detection[J]. Food Science,2021,42(5):314−320. doi: 10.7506/spkx1002-6630-20200226-287
[18]	商迎辉, 章丽娜, 李梦洁, 等. 微生物法测定婴幼儿配方食品中生物素的不确定度评估[J]. 中国食品学报,2019,19(10):221−226. [SHANG Y H, ZHANG L N, LI M J, et al. Uncertainty evaluation for microbiological assay of biotin in formula[J]. Journal of Chinese Institute of Food Science and Technology,2019,19(10):221−226.
[19]	张小刚, 王霞, 王卫东, 等. 超高效液相色谱-串联质谱法测定复合调味料中爱德万甜的不确定度评定[J]. 食品研究与开发,2020,41(2):156−159. [ZHANG X G, WANG X, WANG W D, et al. Uncertainty evaluation of the determination of advantame in compound seasoning by ultra highperformance liquid chromatography-tandem mass spectrometry[J]. Food Research and Development,2020,41(2):156−159.
[20]	黄旭, 贾宏新, 李辉. 荧光分光光度法测定营养素补充剂中硫胺素含量的测量不确定度评定[J]. 食品科学,2015,36(10):174−178. [HUANG X, JIA H X, LI H. Evaluation of the uncertainty of measurement for determination of thiamine content in nutritional supplements by fluorescence spectrophotometry[J]. Food Science,2015,36(10):174−178. doi: 10.7506/spkx1002-6630-201510035
[21]	李静, 张居舟, 余晓娟, 等. 超高效液相色谱-串联质谱法测定豆芽中植物生长调节剂残留量的不确定度评定[J]. 食品科学,2019,40(10):292−297. [LI J, ZHANG J Z, YU X J, et al. Evaluation of uncertainty in determination of plant growth regulator residues in bean sprouts by ultra performance liquid chromatography-tandem mass spectrometry[J]. Food Science,2019,40(10):292−297. doi: 10.7506/spkx1002-6630-20180323-304
[22]	宁霄, 金绍明, 梁瑞强, 等. 超高效液相色谱-串联质谱法测定猪肉中β-受体激动剂残留量的不确定度分析[J]. 食品科学,2016,38(6):315−320. [NING X, JIN S M, LIANG R Q, et al. Uncertainty evaluation for the determination of β-agonists in pork by ultra performance liquid chromatography-tandem mass spectrometry[J]. Food Science,2016,38(6):315−320.
[23]	胡贝贞, 蔡海江, 宋伟华. 茶叶中氟虫腈等8种农药残留的液相色谱-串联质谱法测定及不确定度评定[J]. 色谱,2012,30(9):889−895. [HU B Z, CAI H J, SONG W H. Determination of eight pesticide residues in tea by liquid chromatography-tandem mass spectrometry and its uncertainty evaluation[J]. Chinese Journal of Chromatography,2012,30(9):889−895.
[24]	李菊颖, 何健, 孔德洋, 等. 液相色谱串联质谱法测定大米中多菌灵残留量的不确定度分析[J]. 食品科学,2015,36(22):169−172. [LI J Y, HE J, KONG D Y, et al. Uncertainty evaluation for the determination of carbendazim in rice by liquid chromatography-tandem mass spectrometry[J]. Food Science,2015,36(22):169−172. doi: 10.7506/spkx1002-6630-201522032
[25]	潘城, 胡朝阳, 吴凌, 等. 高效液相色谱法测定蔬菜中异菌脲残留量的不确定度分析[J]. 食品科学,2017,38(24):235−240. [PAN C, HU ZY, WU L, et al. Uncertainty evaluation for the determination of iprodione residues in vegetables by high performance liquid chromatography[J]. Food Science,2017,38(24):235−240. doi: 10.7506/spkx1002-6630-201724038
[26]	毛永杨, 杨桐, 苏涛, 等. 高效液相色谱-原子荧光光谱联用法测定牛肝菌中无机汞、甲基汞、乙基汞的不确定度评估[J]. 食品科学,2017,38(24):272−277. [MAO Y Y, YANG T, SU T, et al. Uncertainty evaluation of for determining inorganic mercury, methylmercury and ethyl mercury inBoletus by high performance liquid chromatography combined with atomic fluorescence spectrometry[J]. Food Science,2017,38(24):272−277. doi: 10.7506/spkx1002-6630-201724044
[27]	FARRANCE I, FRENKEL R. Uncertainty of measurement: A review of the rules for calculating uncertainty components through functional relationships[J]. The Clinical Biochemist Reviews,2012,33(2):49−75.
[28]	BANERJEE K, OULKAR D P, DASGUPTA S, et al. Validation and uncertainty analysis of a multi-residue method for pesticides in grapes using ethyl acetate extraction and liquid chromatography tandem mass spectrometry[J]. Journal of Chromatography A,2007,1173(2):98−109.
[29]	MI, JIN, LEE, et al. Simultaneous analysis and measurement of uncertainty estimation of six isoflavones in Cheonggukjang by liquid chromatography-electrospray tandem mass spectrometry[J]. Food Chemistry,2019,289:139−144. doi: 10.1016/j.foodchem.2019.02.133
[30]	WALORCZYK S. Validation and use of a QuEChERS-based gas chromatographic-tandem mass spectrometric method for multiresidue pesticide analysis in blackcurrants including studies of matrix effects and estimation of measurement uncertainty[J]. Talanta,2014,120:106−113. doi: 10.1016/j.talanta.2013.11.087
[31]	中国合格评定国家认可委员会. CNAS-GL006: 2019 化学分析中不确定度的评估指南[S]. 北京: 中国计量出版社, 2019. China National Accreditation Service for Conformity Assessment. CNAS-GL006: 2019 Guidance on quantifying uncertainty in chemical analysis[S]. Beijing: China Metrology Press, 2019.
[32]	国家质量监督检验检疫总局. GB/T 27418-2017测量不确定度评定与表示 [S]. 北京: 中国标准出版社, 2017. Administration of Quality Supervision Inspection and Quarantine. GB/T 27418-2017 Evaluation and expression of uncertainty inmeasurement [S]. Beijing: China Standardsm Press, 2017.
[33]	国家质量监督检验检疫总局. JJF 1135-2005化学分析测量不确定度评定[S]. 北京: 中国计量出版社, 2005. Administration of Quality Supervision Inspection and Quarantine. JJF 1135-2005 Evaluation of uncertainty in chemical analysis measurement[S]. Beijing: China Metrology Press, 2005.
[34]	国家质量监督检验检疫总局. GB/T 12806-2011 实验室玻璃仪器单标线容量瓶 [S]. 北京: 中国标准出版社, 2011. Administration of Quality Supervision Inspection and Quarantine. GB/T 12806-2011 Laboratory glassware one-mark volumetric flasks[S]. Beijing: China Standards Press, 2011.
[35]	国家质量监督检验检疫总局. JJG 646-2006移液器检定规程 [S]. 北京: 中国计量出版社, 2006. Administration of Quality Supervision Inspection and Quarantine. JJG 646-2006 Verification regulation of locomotive pipette[S]. Beijing: China Metrology Press, 2006.

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