Detection of 7 <i>β</i>-agonists in Animal-derived Food based on Ionic Liquid and Pre-column Fluorescence Derivatization-High Performance Liquid Chromatography

ZHANG Nan; QIAN Yingwen; WU Lihua; WANG Yanjiao; WANG Lu; MA Yan; HONG Xia; XIONG Minhui

doi:10.13386/j.issn1002-0306.2021030237

Volume 42 Issue 23

Nov. 2021

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Science and Technology of Food Industry > 2021 > 42(23): 296-302. > DOI: 10.13386/j.issn1002-0306.2021030237

ZHANG Nan, QIAN Yingwen, WU Lihua, et al. Detection of 7 β-agonists in Animal-derived Food based on Ionic Liquid and Pre-column Fluorescence Derivatization-High Performance Liquid Chromatography[J]. Science and Technology of Food Industry, 2021, 42(23): 296−302. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021030237.

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Detection of 7 β-agonists in Animal-derived Food based on Ionic Liquid and Pre-column Fluorescence Derivatization-High Performance Liquid Chromatography

ZHANG Nan^{1, 2,},
QIAN Yingwen^{1, 2},
WU Lihua^{1, 2},
WANG Yanjiao^{1, 2},
WANG Lu^{1, 2},
MA Yan^{1, 2},
HONG Xia^{1, 2},
XIONG Minhui^{1, 2, ,}

1.
Gansu Institute of Business and Technology Co., Ltd., Lanzhou 730010, China
2.
Gansu Zhongshang Food Quality Test and Detection Co., Ltd., Lanzhou 730010, China

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Received Date: March 18, 2021
Available Online: September 21, 2021

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Abstract

Abstract

In order to enhance the fluorescence intensity of 7 β-agonists, the fluorescent derivatizer 2-(4-sulfoyl chloro-phenyl) benzimidazole reacted with β-agonists, a high performance liquid chromatography-fluorescence detection method for 7 β-agonists in animal-derived food was established. Effects of pH of carbonate buffer solution, reaction temperature, reaction time and concentration of 2-(4-sulfoyl chloro-phenyl) benzimidazole on the fluorescence derivatization were investigated. The results showed that 7 β-agonists had a good linearity in the range of 1.0~100.0 ng/mL and the correlation coefficient (R²) was higher than 0.999 under the optimal conditions. The limits of detection for 7 β-agonists were about 0.30 μg/kg, and the limits of quantitation were less than 1.0 μg/kg. The recoveries of blank pork, mutton and beef samples at three different concentrations of 1.0, 10.0 and 50.0 μg/kg were 81.3%~107.3%, 80.5%~110.7% and 80.2%~105.1%, the intra-day and the inter-day relative standard deviations were 1.48%~13.51%, 1.26%~12.33%, 1.23%~11.49% and 2.21%~11.54%, 1.82%~13.31%, 1.07%~13.21%, respectively. Therefore, the developed method is suitable for determination of 7 β-agonists in animal-derived food qualitatively and quantitatively with satisfactory sensitivity, selectivity, accuracy and precision.

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