ZHANG Yao, JIKE Chizuomo, FENG Zhiping, et al. Screening of Alkaline Tolerant Xylanase Strains in Rhizomyidae Intestines and Study on Enzymatic Properties[J]. Science and Technology of Food Industry, 2021, 42(23): 118−125. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021030231.
Citation: ZHANG Yao, JIKE Chizuomo, FENG Zhiping, et al. Screening of Alkaline Tolerant Xylanase Strains in Rhizomyidae Intestines and Study on Enzymatic Properties[J]. Science and Technology of Food Industry, 2021, 42(23): 118−125. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021030231.

Screening of Alkaline Tolerant Xylanase Strains in Rhizomyidae Intestines and Study on Enzymatic Properties

More Information
  • Received Date: March 18, 2021
  • Available Online: September 28, 2021
  • In order to obtain xylanase with good acid and alkali resistance, to solve the application problem of xylanase in the actual industry. In this study, xylan was used as the sole carbon source. From the intestines and feces of Yibin bamboo rats, the Congo red fading method was used to determine the xylanase activity for re-screening, and one strain with alkaline xylan was screened. Carbohydrase activity strain JZF, 16S rDNA sequence analysis, identified as Bacillus mycoides, the growth curve of Bacillus mycoides JZF, the enzyme production curve and the enzymatic properties of alkaline xylanase explore. The results showed that the enzyme activity of this strain reached 15.17 U/mL after cultured at 37 ℃ and 180 r/min for 48 h; the amount of bacteria reached the maximum after 28 h, the xylanase activity reached the maximum after 72 h, and the most suitable reaction temperature for the produced xylanase was 50 ℃, and the optimum pH was 9.0; the relative enzyme activity could be maintained above 60% under the conditions of 40~60 ℃ and pH8.0~9.0. The metal ions Mn2+ and Ca2+ had obvious effects on the xylanase of this strain. The xylanase produced by the strain obtained in this study has good activity under alkaline conditions, which provides a source and data basis for the subsequent practical application of alkaline xylanase.
  • [1]
    SUREN S, MADLALA A M, PRIOR B A. Thermomyces lanuginosus: Properties of strains and their hemicellulases[J]. FEMS Microbiology Reviews,2003,27(1).
    [2]
    曹要玲, 白晓婷, 刘辉, 等. 1株产碱性木聚糖酶的芽胞杆菌的分离鉴定及其相关研究[J]. 畜牧与兽医,2006(9):11−14. [CAO Yaoling, BAI Xiaoting, LIU Hui, et al. Isolation and identification of an alkali-tolerante Bacillus sp. strain producing xylanse[J]. Animal Husbandry & Veterinary Medicine,2006(9):11−14.
    [3]
    宋玉伟. 产碱性木聚糖酶菌株的选育[D]. 济南: 山东轻工业学院, 2012.

    SONG Yuwei. Screening of alkaline xylanase-producing strains[D]. Jinan: Shandong Polytechnic University, 2012
    [4]
    孙超, 陈卫平. 微生物木聚糖酶及其应用研究进展[J]. 中国酿造,2013,32(4):24−29. [SUN Chao, CHEN Weiping. Research progress in microorganism xylanase and its application[J]. China Brewing,2013,32(4):24−29.
    [5]
    LOUW C, GRANGE D L, PRETORIUS I S, et al. The effect of polysaccharide-degrading wine yeast transformants on the efficiency of wine processing and wine flavour[J]. Journal of Biotechnology,2006,125(4).
    [6]
    PANIAGO P A T, ZANDONADE V R, PICCOLO M A G, et al. Engineered GH11 xylanases from Orpinomyces sp. PC-2 improve techno-functional properties of bread dough[J]. Journal of the Science of Food and Agriculture, 2018.
    [7]
    滕超, 鹿发展, 范光森, 等. 木聚糖酶的研究进展及其在食品领域的应用[J]. 生物产业技术,2019(4):34−41. [TENG Chao, LU Fazhan, FAN Guangsen, et al. Research progress of xylanase and its application in food[J]. Biotechnology & Business,2019(4):34−41.
    [8]
    温博婷, 孙丽超, 王凤忠, 等. 微生物木聚糖酶的研究进展及其在食品领域的应用[J]. 生物产业技术,2017(5):81−86. [WEN Boting, SUN Lichao, WNAG Fengzhong, et al. Research progress of microbial xylanase and its application in food[J]. Biotechnology & Business,2017(5):81−86.
    [9]
    SUNNA A, ANTRANIKIAN G. Xylanolytic enzymes from fungi and bacteria[J]. Critical Reviews in Biotechnology,1997,17(1).
    [10]
    吴仁智, 黄俊, 芦志龙, 等. 酸性木聚糖酶产生菌XYW5的筛选及酶学性质[J]. 广西科学,2018,25(6):669−677. [WU Renzhi, HUANG Jun, LU Zhilong, et al. Screening of microbial strain XYW5 for acidic xylanase and enzymatic properties of crude acidic xylanase[J]. Guangxi Sciences,2018,25(6):669−677.
    [11]
    高志强, 李茜, 徐岳松, 等. 高产木聚糖酶细菌的筛选、鉴定及其部分酶学特性[J]. 应用与环境生物学报,2017,23(3):443−447. [GAO Zhiqiang, LI Xi, XU Yuesong, et al. Isolation and identification of a xylanase-producing bacteriunm and its enzymatic characteristics[J]. Chinese Journal of Applied and Environmental Biology,2017,23(3):443−447.
    [12]
    KALININA A N, BORSHCHEVSKAYA L N, GORDEEVA T L, et al. Screening and taxonomic characterization of xylanase bacterial producers[J]. Pleiades Publishing,2018,54(7).
    [13]
    郑虹, 李莉娟, 童秋霞, 等. 一株高产木聚糖酶菌株的筛选鉴定及酶学特性研究[J]. 食品工业科技,2018,39(6):126−129. [ZHENG Hong, LI Lijuan, TONG Qiuxia, et al. Screening, indetification and enzymatic properties of the high xylanase producing stain[J]. Science and Technology of Food Industry,2018,39(6):126−129.
    [14]
    高雅君, 丁长河. 木聚糖酶在食品工业中的应用研究进展[J]. 粮食与食品工业,2017,24(2):32−36. [GAO Yajun, DING Changhe. Research progress on the application of xylanase in food industry[J]. Cereal and Food Industry,2017,24(2):32−36.
    [15]
    王月琳, 吴玉婷, 石玉. 一株产木聚糖酶菌株的筛选鉴定及产酶条件优化[J]. 安徽农业科学,2019,47(8):168−170,173. [WANG Yuelin, WU Yuting, SHI Yu. Screening and identification of xylanase producing strains and condition optimization for xylanase production[J]. Journal of Anhui Agricultural Sciences,2019,47(8):168−170,173.
    [16]
    袁鸣一, 董佳莹, 陈爱玲, 等. 嗜碱细菌Cellulomonas bogoriensis 69B4~(T)木聚糖酶的表达纯化及性质研究 [J]. 微生物学报: 1−17[2021-09-16]. http://gffiya5911374163c4ac6suqpo5cvfqwf56x9p.fffb.suse.cwkeji.cn:999/10.13343/j.cnki.wsxb.20200748.

    YUAN Mingyi, DONG Jiayin, CHEN Ailin, et al. Expression, purification and properties of Alkaline bacteria Cellulomonas bogoriensis 69B4T xylanase[J]. Acta Microbiologica Sinica: 1−17[2021-09-16]. http://gffiya5911374163c4ac6suqpo5cvfqwf56x9p.fffb.suse.cwkeji.cn:999/10.13343/j.cnki.wsxb.20200748.
    [17]
    周宁. 产酸性木聚糖酶链霉菌的鉴定、产酶条件优化及其基因克隆与表达[D]. 杨凌: 西北农林科技大学, 2016.

    ZHOU Ning. Identification of acid xylanase-producing Streptomyces, optimization of enzyme-producing conditions, and gene cloning and expression[D]. Yangling: Northwest A&F University, 2016.
    [18]
    张世敏, 黄喜海, 徐淑霞, 等. 产酸性木聚糖酶细菌的筛选及产酶条件研究[J]. 安徽农业科学,2013,41(9):3772−3773, 3780. [ZHANG Shimin, HUANG Xihai, XU Suxia, et al. Study on screening of acidic xylanase producing bacteria and its fermentation conditions[J]. Journal of Anhui Agricultural Sciences,2013,41(9):3772−3773, 3780.
    [19]
    董桓. 纤维素酶和半纤维素酶高产菌株的筛选[D]. 保定: 河北大学, 2014.

    DONG Huan. Screening for cellulase and hemicellulase high-yield strains[D]. Baoding: Hebei University, 2014.
    [20]
    石乾乾. 纤维素酶和半纤维素酶高产菌株的筛选及其在生物质降解中的应用[D]. 上海: 华东理工大学, 2011.

    SHI Qianqian. Screening of high-yield cellulase and hemicellulase strains and their application in biomass degradation[D]. Shanghai: East China University of Science and Technology, 2011.
    [21]
    曹慧, 张会会, 张腾月, 等. 产低温纤维素酶和木聚糖酶真菌的筛选鉴定及酶学性质[J]. 饲料研究: 1−8. [2021-09-16]. http://gffiyc3066c973e9140a1huqpo5cvfqwf56x9p.fffb.suse.cwkeji.cn:999/kcms/detail/11.2114.S.20201224.1754.002.html.

    CAO Hui, ZHANG Huihui, ZHANG Tengyue, et al. Screening and enzymatic properties of cold-adapted cellulose-xylanase degradation fungus[J]. Feed Research: 1−8[2021-09-16]. http://gffiyc3066c973e9140a1huqpo5cvfqwf56x9p.fffb.suse.cwkeji.cn:999/kcms/detail/11.2114.S.20201224.1754.002.html.
    [22]
    王继莲, 陈芸, 李明源, 等. 产低温纤维素酶菌株的分离鉴定及产酶特征研究[J]. 江西农业大学学报,2019,41(2):356−364. [WANG Jilian, CHEN Yun, LI Mingyuan, et al. Screening, identification of a cold-active cellulase strain and characterization of its cellulose production[J]. Acta Agriculturae Universitatis Jiangxiensis,2019,41(2):356−364.
    [23]
    陈娜, 生吉萍, 申琳. 产木聚糖酶但无纤维素酶活性菌株的筛选及初步鉴定[J]. 食品科学,2011,32(17):273−277. [CHEN Na, SHENG Jiping, SHEN Lin. Isolation and preliminarily identification of cellulase-free xylanase-producing strain[J]. Food Science,2011,32(17):273−277.
    [24]
    董延娟. 产木聚糖酶菌株YS1069的鉴定、产酶条件优化和酶学性质的研究[D]. 上海: 上海海洋大学, 2015.

    DONG Yanjuan. Identification fermentation and characterization ofa strain YS1069 producing xylanase[D]. Shanghai: Shanghai Ocean University, 2015
    [25]
    张贝贝, 赵晓兵, 何丽丽, 等. 牛瘤胃细菌产木聚糖酶菌株的筛选及酶学性质研究[J]. 河南农业科学,2014,43(4):128−132. [ZHANG Beibei, ZHAO Xiaobing, HE Lili, et al. Screening of xylanase producing bacteria from bovinerumen and the enzyme properties[J]. Journal of Henan Agricultural Sciences,2014,43(4):128−132.
    [26]
    郑亚伦, 夏瑛, 李良, 等. 源于解淀粉芽孢杆菌酸性木聚糖酶酶学性质的研究[J]. 食品与发酵工业,2020,46(24):58−65. [ZHENG Yalun, XIA Ying, LI Liang, et al. Enzymatic properties of acid resistant xylanase from Bacillus amyloliquefaciens[J]. Food and Fermentation Industries,2020,46(24):58−65.
    [27]
    姜立春, 王成红, 刘羽, 等. 半纤维素降解菌J-25的筛选、鉴定及产酶特性研究[J]. 食品工业科技,2017,38(5):145−150. [JIANG Lichun, WANG Chenghong, LIU Yu, et al. Screening, identification and enzymatic ananlysis of hemicellulose-degrading strain J-25[J]. Science and Technology of Food Industry,2017,38(5):145−150.
    [28]
    徐有权, 顾文杰, 张发宝, 等. 酸性半纤维素降解细菌的筛选与鉴定[J]. 微生物学杂志,2012,32(2):36−40. [XU Youquan, GU Wenjie, ZHANG FAbao, et al. Screening and characterization of acidic hemicellulose-degrading bacteria[J]. Journal of Microbiology,2012,32(2):36−40.
    [29]
    刘维, 徐璐, 陈晓倩, 等. 一株产木聚糖酶菌株的筛选及产酶条件优化[J]. 湖南农业科学,2019(8):5−9. [LIU Wei, XU Lu, CHEN Xiaoqian, et al. Screening of a xylanase-producing strain and optimization of fermentation conditions[J]. Hunan Agricultural Sciences,2019(8):5−9.
    [30]
    易旭东, 潘虎, 田云, 等. 一株碱性木聚糖酶产生菌的筛选及发酵条件的优化[J]. 中国农学通报,2017,33(17):12−18. [YI Xudong, PAN Hu, TIAN Yun, et al. Screening of an alkaline xylanase-producing strain and optimization of fermentation conditions[J]. Chinese Agricultural Science Bulletin,2017,33(17):12−18.
    [31]
    冯波. 木聚糖酶产生菌的筛选、发酵条件优化及其酶学性质研究[D]. 长沙: 湖南农业大学, 2016.

    FENG Bo. Screening of xylanase producing strains, optimization of fermentation and studies on enzymatic property[D]. Changsha: Hunan Agricultural University, 2016.
    [32]
    何敏超, 刘云云, 陈小燕, 等. 木聚糖酶产生菌的筛选、发酵及酶学性质[J]. 中国酿造,2019,38(12):107−111. [HE Minchao, LIU Yunyun, CHEN Xiaoyan, et al. Screening, fermentation and enzymatic properties of xylanase-producing strain[J]. China Brewing,2019,38(12):107−111.
    [33]
    王华广. Clostridium clariflavum木聚糖酶的异源表达及分子改造[D]. 无锡: 江南大学, 2018.

    WANG Huaguang, Heterologous expression and molecular modification of Clostridium clariflavum xylanase[D]. Wuxi: Jiangnan University, 2018.
    [34]
    MUROWANIECKI O D, RAFAELA C B A, JULIANO K S. Diversification of nitrogen sources as a tool to improve endo-xylanase enzyme activity produced by Cryptococcus laurentii[J]. Biocatalysis and Agricultural Biotechnology,2021:32.
    [35]
    MANAMI S, TEISUKE T, KOHEI K, et al. Insight into the mechanism of thermostabilization of GH10 xylanase from Bacillus sp. strain TAR-1 by the mutation of S92 to E[J]. Bioscience, Biotechnology, and Biochemistry,2021,85(2).
  • Cited by

    Periodical cited type(35)

    1. 李秀清,何江龙,李盼盼,纪宝玉,裴莉昕,陈随清,董诚明. 麦冬不同部位红外光谱分析及成分含量的测定. 饲料工业. 2025(03): 112-117 .
    2. 罗欣,胡杨,倪兴婷,谈小飞,黄涅,赖富丽,李强,杨强. 九蒸九制对九华山多花黄精品质的影响. 中国酿造. 2025(02): 144-149 .
    3. 袁怡菁,王秋红. 黄精化学成分、药理作用研究进展及质量标志物预测分析. 中医药信息. 2024(02): 72-80+86 .
    4. 张倜培,李青,包鸿慧,沈正兴,石娟,唐前勇,程一方,周睿. 干燥方式对绿碎茶多酚提取物体外抗氧化和抗糖尿病及乙酰胆碱酯酶抑制活性的影响. 食品与发酵工业. 2024(04): 77-84 .
    5. 罗亚惠,宿珠琳,马永生,邹建. 超声辅助法提取柚子皮多酚的工艺优化. 现代食品. 2024(01): 51-56 .
    6. 黄素艳,崔柯鑫,周德庆,王珊珊,王明丽,王大军. 植物多酚改善肠道屏障及糖脂代谢的研究进展. 食品研究与开发. 2024(06): 218-224 .
    7. 关洋莹,唐守方,彭雨鑫,李梦媛,刘赢心,蒋璥远,冯淑婷,张新民,武毅. 桦褐孔菌抗糖尿病的研究进展. 长春中医药大学学报. 2024(04): 463-467 .
    8. 王爱灵,张梅,申娜娜,徐梦文,章骏伟,李瑞龙,兰伟. 黑果腺肋花楸果酒生产工艺研究进展. 滁州学院学报. 2024(02): 21-28 .
    9. 符兵,周东来,李庆荣,邢东旭. 桑叶及其活性物质对动物肠道微生物的调控作用研究进展. 中国畜牧兽医. 2024(06): 2460-2470 .
    10. 王菁,乔勇进,柳洪入,王晓,叶章颖,林思敏,王春芳. 超高压在植物活性物质提取中的应用研究进展. 农业工程学报. 2024(18): 272-282 .
    11. 陈睿,李佳轩,王旭,谢双龙,覃继肖,许一清,卢琦,田兴舟. 刺梨籽对肉兔消化代谢、血浆生化指标、屠宰性能及肉品质的影响. 动物营养学报. 2024(10): 6673-6684 .
    12. 郭子斌,魏静,范蓓,王凤忠,蔡如玉,孙晶. 辅助降血糖茄皮含片制备. 热带农业科学. 2024(08): 65-74 .
    13. 薛惠丹,张未希,邓诏隆,董瑞杰,陈瑞飞,安靖. 徐香猕猴桃果皮多酚提取工艺的优化及其单体抗氧化活性研究. 陕西科技大学学报. 2024(06): 31-39 .
    14. 那治国,余爽,贺书珍,初众. 低GI杂粮可可冲调粉辅助降血糖作用. 食品工业科技. 2023(01): 28-37 . 本站查看
    15. 陈燕萌,魏金兰,陈凤,黄宇扬,李珊珊,郭松. 假蒟叶多酚提取工艺及其抗氧化活性研究. 中国食品添加剂. 2023(02): 76-84 .
    16. 邱建强,黄文,王玉玺,彭凯. 单宁对动物肠道微生物调控的研究进展. 动物营养学报. 2023(02): 772-781 .
    17. 陈晓晨,杜希萍,周莉鹃,伍菱,李志朋,杨远帆. 坛紫菜多酚工艺优化及降血糖和抗氧化活性研究. 食品与机械. 2023(02): 140-146 .
    18. 周爽,黄莉,王彦美,张韩杰,赵莉,贺子阳,孙素洁. 植物多酚的生物利用度及其降血糖活性研究进展. 食品研究与开发. 2023(06): 211-217 .
    19. 沈晓静,袁文娟,邵俊文,文午,赵品材,洪金琪,顾春燕,姜薇薇. 云南小粒咖啡生豆的抗氧化及α-淀粉酶抑制活性研究. 中国食品添加剂. 2023(05): 96-102 .
    20. 师英春,廖森泰,杨琼,邹宇晓,李倩. 植物多酚和多糖经肠道微生态途径互作调节糖脂代谢的研究进展. 食品安全质量检测学报. 2023(08): 109-118 .
    21. 杨淑珺,胡睿智,贺建华,伍树松. 植物多酚对脂肪酸代谢的调控作用及潜在机制. 中国畜牧杂志. 2023(07): 14-20 .
    22. 史凡,杨红. 纳米结构脂质载体制备及在功能食品中的应用研究进展. 食品工业科技. 2023(15): 1-6 . 本站查看
    23. 陈鑫,王晨,王黎明,赵抒娜,赵芸,刘孟涛,孟庆佳. L-阿拉伯糖复配糖对小鼠血糖的影响. 食品工业科技. 2023(17): 392-398 . 本站查看
    24. 李琳琳,王乐,尹卫,代爽,刘晓军,梁健,王煜伟. 紫皮大蒜多酚分离纯化及体外降血糖活性研究. 中国调味品. 2023(09): 18-23 .
    25. 王露,黄立新,刘磊. 食源性多酚对α-淀粉酶作用大米淀粉活性的影响. 中国粮油学报. 2023(08): 131-137 .
    26. 唐双庆,陈禅友,王亚珍,王红波. 食用豆类资源中酚类化合物研究进展. 中国调味品. 2023(12): 200-205+212 .
    27. 徐小云,伍舒华,赖敏成,廖延智,吴文,欧纯宜. 高效液相色谱测定龙眼核中多酚含量方法优化. 广州化工. 2023(22): 61-64 .
    28. 彭言劼,魏雪莲,周勇,胡强,胡霞,宋春草. 不同果袋对红富士葡萄果实品质的影响. 中国南方果树. 2022(02): 134-138 .
    29. 陈旭,徐楚炎,范露,石玉. 山楂叶多酚提取工艺及其降糖降脂应用研究. 饲料研究. 2022(05): 78-83 .
    30. 滕欢欢,王仁中,吴德玲,金传山,柳春风,唐旭,黄圣卓,许凤清. 多花黄精炮制前后不同极性部位抗氧化与降血糖活性研究. 食品与发酵工业. 2022(08): 70-75 .
    31. 吴卫成,忻晓庭,张程程,刘大群,卢立志,胡宏海,章检明,张治国,郭阳. 番薯叶多酚提取工艺优化及其生物活性研究. 中国食品学报. 2022(05): 189-199 .
    32. 胡德胜,李健,张岩,钟丹苗,方雪飞,廖营忠,苏干光. 林下栽培的大球盖菇多酚提取工艺条件优化. 中国食用菌. 2022(08): 65-70 .
    33. 张杰,党斌,杨希娟. 植物多酚的生理活性、抑菌机理及其在食品保鲜中的应用研究进展. 食品工业科技. 2022(24): 460-468 . 本站查看
    34. 马雪,赵丹,张瑞,琚艳君,赵多勇. 多酚类化合物检测分析方法研究进展. 食品安全质量检测学报. 2021(11): 4575-4582 .
    35. 高若容,王钿烈,黄雪松. 固相萃取-高效液相色谱法同时测定蒜皮中六种酚类物质. 食品与发酵工业. 2021(22): 266-272 .

    Other cited types(23)

Catalog

    Article Metrics

    Article views PDF downloads Cited by(58)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return