Rapid Screening of 30 Plant Growth Regulators and Quinolone Antibiotics in Bean Sprout Samples

HUANG Yanhong; JIANG Xiang; PAN Zhenghua; SU Haiyan; JIANG Dingzhi; LIANG Feiyan

doi:10.13386/j.issn1002-0306.2021030198

Volume 42 Issue 23

Nov. 2021

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Science and Technology of Food Industry > 2021 > 42(23): 287-295. > DOI: 10.13386/j.issn1002-0306.2021030198

HUANG Yanhong, JIANG Xiang, PAN Zhenghua, et al. Rapid Screening of 30 Plant Growth Regulators and Quinolone Antibiotics in Bean Sprout Samples[J]. Science and Technology of Food Industry, 2021, 42(23): 287−295. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021030198.

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Rapid Screening of 30 Plant Growth Regulators and Quinolone Antibiotics in Bean Sprout Samples

Guangxi-Asean Food Inspection Center, Nanning 530021, China

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Received Date: March 15, 2021
Available Online: October 10, 2021

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Abstract

Abstract

A method was developed for the simultaneous determination of 30 plant growth regulators residues and quinolone antibiotics residues in bean sprouts by ultra-high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) with pass-through solid-phase extraction. The samples were extracted by 90% acetonitrile containing 0.1% formic acid and purified with PRiME HLB solid-phase extraction, gradient elution with acetonitrile and water (containing 0.1% formic acid). The target compounds were separated on an Agilent SB-Aq RRHD column, then detected by electrospray positive and negative ion multiple reaction monitoring mode. The target compounds were quantified by using the matrix-matched standard calibration. The result showed that the correlation coefficients greater than 0.991 in the range of 2~500 μg/L, the detection limit was 0.04~1.67 μg/kg, the quantitative limit was 0.1~5.6 μg/kg, the average recovery was 74.0%~113.5%, and the relative standard deviation was 0.6%~7.7% (n=6). The method is simple and sensitive, can be used for rapid screening of 30 plant growth regulators and quinolones in bean sprouts.

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Rapid Screening of 30 Plant Growth Regulators and Quinolone Antibiotics in Bean Sprout Samples