Fermentation Flavor Characteristics and Chemical Characterization of <i>Lactococcus lactis</i> in Commercial Starters

XIE Xiang; GONG Pimin; LIU Ao; ZHANG Hongwei; TONG Lingjun; YI Huaxi; WANG Yawei; LIU Tongjie; ZHANG Lanwei

doi:10.13386/j.issn1002-0306.2021030196

Volume 42 Issue 19

Sep. 2021

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Science and Technology of Food Industry > 2021 > 42(19): 152-162. > DOI: 10.13386/j.issn1002-0306.2021030196

XIE Xiang, GONG Pimin, LIU Ao, et al. Fermentation Flavor Characteristics and Chemical Characterization of Lactococcus lactis in Commercial Starters[J]. Science and Technology of Food Industry, 2021, 42(19): 152−162. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021030196.

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Fermentation Flavor Characteristics and Chemical Characterization of Lactococcus lactis in Commercial Starters

1.
College of Food Science and Engineering, Ocean University of China, Qingdao 266000, China
2.
Technology Center of Qingdao Customs District, Qingdao 266000, China
3.
Beijing Doit Biotechnology Co., Ltd., Beijing 100000, China

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Received Date: March 15, 2021
Available Online: August 09, 2021

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Abstract

Abstract

In order to deeply analyze the flavor contribution and characteristic aroma components of Lactococcus lactis in fermented milk, twenty-one strains of Lactococcus lactis were isolated from different commercial starters, and subjected to single-strain fermentation. The aroma type was defined according to the results of sensory evaluation, and then determination of volatile flavor compounds in fermented milk was performed using GC-IMS technology, and finally flavor substances were analyzed by chemometric method. The results showed that these single-strain fermented milk can be divided into three flavor types: Milk flavor, cream flavor, and butter flavor. Twenty-five compounds were identified by GC-IMS, including nine alcohols, eight ketones, three aldehydes, one carboxylic acid, and four esters. By drawing the GC-IMS fingerprint map, the characteristic compound composition of fermented milk from different strains was presented. The fermented milks of four strains of Lactococcus lactis subsp. lactis var. diacetylactis were well distinguished by principal component analysis, and the results were consistent with the sensory evaluation results. The correlation between sensory flavor classification and volatile components was analyzed by PLSR, and it was found that the volatile flavor components that had a greater contribution to the “butter flavor” were 1-hexanal and 1-hexanol, the compound which had a greater contribution to the “creamy flavor” was ethyl acetate, the compounds which contributed to the “milk flavor” were obscure, and further analysis was needed for revealing the key ingredients. This research provides a reference for exerting the flavor enhancement effect of Lactococcus lactis in fermented milk.
- Lactococcus lactis,
- fermented milk,
- flavor ingredient,
- fragrance,
- gas chromatograph-ion mobility spectrometry (GC-IMS)

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References

[1]	高雨婷, 秦安琪, 钱建瑛, 等. 奶味香精的制备技术与开发现状[J]. 中国乳品工业,2021,49(1):35−38, 45. [Gao Y T, Qin A Q, Qian J Y, et al. Production technology and development status of milk flavors[J]. China Dairy Industry,2021,49(1):35−38, 45.
[2]	Ott A, Hugi A, Baumgartner M, et al. Sensory investigation of yogurt flavor perception: Mutual influence of volatiles and acidity[J]. Journal of Agricultural and Food Chemistry,2000,48(2):441−450. doi: 10.1021/jf990432x
[3]	Chen C, Zhao S, Hao G, et al. Role of lactic acid bacteria on the yogurt flavour: A review[J]. International Journal of Food Properties,2017,20(sup1):S316−S330. doi: 10.1080/10942912.2017.1295988
[4]	De Bok F A M, Janssen P W M, Bayjanov J R, et al. Volatile compound fingerprinting of mixed-culture fermentations[J]. Applied Environmental Microbiology,2011,77(17):6233−6239. doi: 10.1128/AEM.00352-11
[5]	Pan D D, Wu Z, Peng T, et al. Volatile organic compounds profile during milk fermentation by Lactobacillus pentosus and correlations between volatiles flavor and carbohydrate metabolism[J]. Journal of Dairy Science,2014,97(2):624−631. doi: 10.3168/jds.2013-7131
[6]	Neves A R, Pool W A, Kok J, et al. Overview on sugar metabolism and its control in Lactococcus lactis-The input from in vivo NMR[J]. Fems Microbiology Reviews,2005,29(3):531−554.
[7]	Zuljan F A, Mortera P, Alarcón S H, et al. Lactic acid bacteria decarboxylation reactions in cheese[J]. International Dairy Journal,2016:53−62.
[8]	赵羽, 陆林, 刘小鸣, 等. 基于蔗糖利用能力对豆乳发酵菌株的筛选及其应用[J]. 食品与发酵工业,2020,46(9):43−49. [Zhao Y, Lu L, Liu X M, et al. Screening of lactic acid bacteria for soymilk fermentation based on sucrose utilization ability[J]. Food and Fermentation Industries,2020,46(9):43−49.
[9]	李柏良, 刘飞, 于上富, 等. 有氧呼吸对乳酸乳球菌代谢途径的影响及其潜在应用展望[J]. 食品工业科技,2016,37(19):372−376. [Li B L, Liu F, Yu S F, et al. Impact of aerobic respiration on metabolic pathway of Lactococcus lactis and the prospect of potential application[J]. Science and Technology of Food Industry,2016,37(19):372−376.
[10]	Backus L, Wels M, Boekhorst J, et al. Draft genome sequences of 24 Lactococcus lactis strains[J]. Microbiology Resource Announcements,2017,5(13):e01737−01716.
[11]	Ruggirello M, Giordano M, Bertolino M, et al. Study of Lactococcus lactis during advanced ripening stages of model cheeses characterized by GC-MS[J]. Food Microbiology,2018,74:132−142. doi: 10.1016/j.fm.2018.03.012
[12]	Kok J, Van Gijtenbeek L A, De Jong A, et al. The evolution of gene regulation research in Lactococcus lactis[J]. Fems Microbiology Reviews,2017,41:S220−S243. doi: 10.1093/femsre/fux028
[13]	Zeidan A A, Poulsen V K, Janzen T, et al. Polysaccharide production by lactic acid bacteria: from genes to industrial applications[J]. Fems Microbiology Reviews,2017,41:S168−S200. doi: 10.1093/femsre/fux017
[14]	Wang S, Chen H, Sun B. Recent progress in food flavor analysis using gas chromatography-ion mobility spectrometry (GC-IMS)[J]. Food Chem,2020,315:126158. doi: 10.1016/j.foodchem.2019.126158
[15]	李娟, 任芳, 甄大卫, 等. 气相色谱-离子迁移谱分析几种乳制品挥发性风味化合物[J]. 食品科学,2020:1−11. [Li J, Ren F, Zhen D W, et al. Analysis of volatile flavor compounds in several dairy products by gas chromatography-ion mobility spectrometry[J]. Food Science,2020:1−11. doi: 10.7506/spkx1002-6630-20190127-346
[16]	Feng D, Wang J, Ji X J, et al. HS-GC-IMS detection of volatile organic compounds in yak milk powder processed by different drying methods[J]. Lwt,2021:141.
[17]	布坎南, 吉本斯. 伯杰细菌鉴定手册[M]. 8版. 北京: 科学出版社, 1984.
[18]	Kazancigil E, Demirci T, Ozturk-Negis H I, et al. Isolation, technological characterization and in vitro probiotic evaluation of Lactococcus strains from traditional Turkish skin bag Tulum cheeses[J]. Annals of Microbiology,2019,69(12):1275−1287. doi: 10.1007/s13213-019-01512-4
[19]	黄蓉, 张学亮, 韩烁, 等. 瑞士乳杆菌MB2-1源胞外多糖对10种益生菌生长特性的影响[J]. 食品科学,2020,41(6):163−169. [Huang R, Zhang X L, Han S, et al. Effect of exopolysaccharides of Lactobacillus helveticus MB2-1 on growth characteristics of ten probiotics[J]. Food Science,2020,41(6):163−169. doi: 10.7506/spkx1002-6630-20190811-124
[20]	Li C, Song J, Kwok L-Y, et al. Influence of Lactobacillus plantarum on yogurt fermentation properties and subsequent changes during postfermentation storage[J]. Journal of Dairy Science,2017,100(4):2512−2525. doi: 10.3168/jds.2016-11864
[21]	Huang Z, Huang L, Xing G, et al. Effect of co-fermentation with lactic acid bacteria and K. marxianus on physicochemical and sensory properties of goat milk[J]. Foods,2020,9(3):299. doi: 10.3390/foods9030299
[22]	Liu W, Li H, Jiang D, et al. Effect of Saccharomyces cerevisiae, Torulaspora delbrueckii and malolactic fermentation on fermentation kinetics and sensory property of black raspberry wines[J]. Food Microbiol,2020,91:103551. doi: 10.1016/j.fm.2020.103551
[23]	Zeng X, Liu J, Dong H, et al. Variations of volatile flavour compounds in Cordyceps militaris chicken soup after enzymolysis pretreatment by SPME combined with GC-MS, GC×GC-TOF MS and GC-IMS[J]. International Journal of Food Science Technology,2020,55(2).
[24]	Gallegos J, Arce C, Jordano R, et al. Target identification of volatile metabolites to allow the differentiation of lactic acid bacteria by gas chromatography-ion mobility spectrometry[J]. Food Chemistry,2017,220:362−370. doi: 10.1016/j.foodchem.2016.10.022
[25]	袁志鹰, 黄惠勇, 谢梦洲, 等. 应用顶空-气相离子迁移谱分析百合发酵乳的风味物质[J]. 理化检验-化学分册,2020,56(1):78−83. [Yuan Z Y, Huang H Y, Xie M Z, et al. Analysis of flavoring substances in fermented lily milk by HS-GC-IMS[J]. Physical Testing and Chemical Analysis (Part B: Chemical Analysis),2020,56(1):78−83.
[26]	Tong L, Yi H, Wang J, et al. Effect of preheating treatment before defatting on the flavor quality of skim milk[J]. Molecules,2019,24(15):2824. doi: 10.3390/molecules24152824
[27]	姜陈波, 杭锋. 双歧杆菌和乳酸菌β-半乳糖苷酶转糖基作用的研究进展[J]. 食品科学,2019,40(7):335−341. [Jiang C B, Hang F. Advances in our understanding of the transgalactosylation activity of β-galactosidase from biﬁdobacteria and lactic acid bacteria[J]. Food Science,2019,40(7):335−341. doi: 10.7506/spkx1002-6630-20180302-024
[28]	Cheng Hefa. Volatile flavor compounds in yogurt: A review[J]. Critical Reviews in Food Science Nutrition,2010,50(10):938−950. doi: 10.1080/10408390903044081
[29]	Routray W, Mishra H N. Scientific and technical aspects of yogurt aroma and taste: A review[J]. Comprehensive Reviews in Food Science and Food Safety,2011,10(4):208−220. doi: 10.1111/j.1541-4337.2011.00151.x
[30]	张国农, 顾敏锋, 李彦荣, 等. SPME-GC/MS测定再制干酪中的风味物质[J]. 中国乳品工业,2006,34(9):52−56. [Zhang G N, Gu M F, Li Y R, et al. Determination of flavor substances in processed cheese by SPME-GC/MS[J]. China Dairy Industry,2006,34(9):52−56. doi: 10.3969/j.issn.1001-2230.2006.09.014
[31]	Güler Z. Changes in salted yoghurt during storage[J]. International Journal of Food Science Technology,2007,42(2):235−245. doi: 10.1111/j.1365-2621.2006.01505.x
[32]	Østlie H M, Helland M H, Narvhus J A. Growth and metabolism of selected strains of probiotic bacteria in milk[J]. International Journal of Food Microbiology,2003,14(1-2):957−965.
[33]	武士美, 靳汝霖, 任为一, 等. 德氏乳杆菌保加利亚亚种发酵乳中挥发性风味物质的比较分析[J]. 中国乳品工业,2017,45(9):4−10. [Wu S M, Jin R L, Ren W Y, et al. Comparision and analysis of Lactobacillus delbrueckii subsp. bulgaricus on producing volatile compounds in fermented milk[J]. China Dairy Industry,2017,45(9):4−10. doi: 10.3969/j.issn.1001-2230.2017.09.001
[34]	Dan T, Jin R, Ren W, et al. Characteristics of milk fermented by Streptococcus thermophilus MGA45-4 and the profiles of associated volatile compounds during fermentation and storage[J]. Molecules,2018,23(4):878. doi: 10.3390/molecules23040878
[35]	Li W, Ren M, Duo L, et al. Fermentation characteristics of Lactococcus lactis subsp. lactis isolated from naturally fermented dairy products and screening of potential starter isolates[J]. Frontiers in Microbiology,2020:11.
[36]	Zhang L, Mi S, Liu R, et al. Evaluation of volatile compounds in milks fermented using traditional starter cultures and probiotics based on odor activity value and chemometric techniques[J]. Molecules,2020,25(5).
[37]	耿明雪, 刘小鸣, 赵建新, 等. 基于组学及感官评价的酸奶风味研究[J]. 食品与发酵工业,2018,44(7):250−257. [Geng M X, Liu X M, Zhao J X, et al. Investigate the flavor characteristics of yogurt through metabonomics and sensory evaluation[J]. Food and Fermentation Industries,2018,44(7):250−257.
[38]	Lu Y, Chan L J, Li X, et al. Effects of different inoculation strategies of Saccharomyces cerevisiae and Williopsis saturnus on chemical components of mango wine[J]. Lwt-Food Science and Technology,2018,87:85−92. doi: 10.1016/j.lwt.2017.08.074

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