Rapid Determination of Five Alkaloids in Poppy Shell in Herbal Tea by HPLC-MS/MS

LIU Minmin; XIE Xiaoxia; CHEN Qiao; HU Bibo

doi:10.13386/j.issn1002-0306.2021020209

Volume 42 Issue 23

Nov. 2021

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Science and Technology of Food Industry > 2021 > 42(23): 266-272. > DOI: 10.13386/j.issn1002-0306.2021020209

LIU Minmin, XIE Xiaoxia, CHEN Qiao, et al. Rapid Determination of Five Alkaloids in Poppy Shell in Herbal Tea by HPLC-MS/MS[J]. Science and Technology of Food Industry, 2021, 42(23): 266−272. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021020209.

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Rapid Determination of Five Alkaloids in Poppy Shell in Herbal Tea by HPLC-MS/MS

1.
Guangdong Institute for Drug Control, Guangzhou 510663, China
2.
School of Traditional Chinese Materia Medica, Guangdong Food and drug Vocational College, Guangzhou 510520, China

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Received Date: February 25, 2021
Available Online: October 07, 2021

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Abstract

Abstract

Objective: A HPLC-MS/MS method was developed for rapid determination of five alkaloids in Poppy Shell in herbal tea, including morphine, codeine, thebaine, papaverine, and narcotine. Method: Agilent Poroshell 120 EC-C₁₈（2.1 mm×100 mm, 2.7 μm）was applied with the mobile phase consisting of 5 mmol/L ammonium acetate containing 0.1 % acetic acid-methanol by gradient elution. The electrospray ion source was operated in the positive ion mode using the multi-reaction monitoring (MRM) method, and the results were quantified by the external standard method. Result: The concentration range was 2.5～100 ng/mL in morphine and codeine, 0.5～20 ng/mL in the others. The correlation coefficients (R) were all above 0.995. The detection limit was 0.8 μg/g in morphine and codeine, 0.2 μg/g in the others. The average recoveries ranged from 84.5% to 105.1% in liquid samples, with relative stand deviations (RSDs) all below 6.3% (n=6), ranged from 72.5% to 112.3% in solid samples, with relative stand deviations (RSDs) all below 7.1% (n=6). 150 Batches of samples bought from markets were screened, and 6 batches showed positive for these analytes. Conclusion: This method was simple, accurate, could be used for the determination of five alkaloids in Poppy Shell added in herbal tea.
- herbal tea,
- alkaloids in Poppy Shell,
- high performance liquid chromatography-tandem mass spectrometry,
- morphine,
- codeine,
- thebaine,
- papaverine,
- narcotine

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