LAI Min, WU Tong, XIE Kui, et al. Comparison of Lipids in Four Kinds of Milk Powder of Cow and Goat[J]. Science and Technology of Food Industry, 2021, 42(22): 62−71. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021020193.
Citation: LAI Min, WU Tong, XIE Kui, et al. Comparison of Lipids in Four Kinds of Milk Powder of Cow and Goat[J]. Science and Technology of Food Industry, 2021, 42(22): 62−71. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021020193.

Comparison of Lipids in Four Kinds of Milk Powder of Cow and Goat

More Information
  • Received Date: February 23, 2021
  • Available Online: September 13, 2021
  • Objective: To compare and analyze the lipid characteristics of common and organic milk powder. Methods: Taking common milk powder, common goat milk powder, organic milk powder and organic goat milk powder as research objects, lipids in milk powder were extracted by chloroform methanol method, triglyceride and phospholipid were separated by aminopropyl silica gel solid phase extraction column, Sn-2 monoglyceride was obtained by hydrolyzing 1,3-fatty acid of triglyceride molecule with porcine pancreatic lipase, and Sn-2 fatty acid was obtained by hydrolyzing phospholipid with phospholipase A2. The composition and content of triglycerides and phospholipids were analyzed by gas chromatography, the configuration of triglycerides was analyzed by ultra-high performance liquid chromatography coupled with mass spectrometry, and the composition and content of phospholipids and cholesterol were determined by high performance liquid chromatography. Results: The contents of lipid, triglyceride and phospholipid in goat milk powder were higher than those in cow milk powder, and the content of common milk powder was higher than that of organic milk powder. Palmitic acid was the main saturated fatty acid and oleic acid was the main monounsaturated fatty acid in milk powder, and the content of common goat milk powder was significantly higher than the other three kinds of milk powder(P<0.05). The content of n-6 series polyunsaturated fatty acids in ordinary goat milk powder was the highest, and the content of n-3 series polyunsaturated fatty acids in organic goat milk powder was the highest. The four kinds of milk powder Sn-2 fatty acids were mainly saturated fatty acids, and lauric acid, myristic acid and palmitic acid were more likely to be distributed in the Sn-2 position of triglycerides, and palmitic acid was also more likely to be distributed in the Sn-2 position of phospholipids. The medium chain triglycerides(MCT) of four kinds of milk powder were mainly composed of Ca-Ca-Ca(Ca:C10:0) and Ca-Ca-La(La:C12:0), and the MCT of goat milk powder was higher than that of cow milk powder. The contents of medium and long chain triglycerides(MLCT) in goat milk powder were higher than those in cow milk powder(P: C16:0), Ca-Ca-O(O:C18:0) and la-m-p (M:C14:0) (P<0.05). The highest content of LCT was O-P-O(O:C18:0). Conclusions: The lipid of goat milk powder was more easily absorbed than that of cow milk powder. Compared with ordinary milk powder, organic milk powder had more security, higher n-3 PUFA content and higher nutritional value.
  • [1]
    FONTECHA J, RODRIGUEZ-ALCALA L M, CALVO M V, et al. Bioactive milk lipids[J]. Current Nutrition & Food Science,2011,7(3):155−159.
    [2]
    曾令平, 张继东. 结构脂质及其在乳制品中的应用[J]. 中国乳品工业,2010,38(10):39−42. [ZENG L P, ZHANG J D. Introduction and application of structured lipids in dairy products[J]. China Dairy Industry,2010,38(10):39−42. doi: 10.3969/j.issn.1001-2230.2010.10.011
    [3]
    CONTARINI G, POVOLO M. Phospholipids in milk fat: Composition, biological and technological significance, and analytical strategies[J]. International Journal of Molecular Sciences,2013,14(2):2808−2831. doi: 10.3390/ijms14022808
    [4]
    吕晓玲 王淼. 食品生物化学[M]. 北京: 中国轻工业出版社, 2009.

    LV X L, WANG M. Food Biochemistry[M]. Beijing: China Light Industry Press, 2009.
    [5]
    王萍, 张银波, 江木兰. 多不饱和脂肪酸的研究进展[J]. 中国油脂,2008,33(12):42−46. [WANG P, ZHANG Y B, JIANG M L. Research progress of polyunsaturated fatty acids[J]. China Oils and Fats,2008,33(12):42−46. doi: 10.3321/j.issn:1003-7969.2008.12.011
    [6]
    BENJAMIN S, SPENER F. Conjugated linoleic acids as functional food: An insight into their health benefits[J]. Nutrition & Metabolism,2009,6(1):36−36.
    [7]
    董世华. 羊奶的营养价值[J]. 食品与健康,2001(4):19. [DONG S H. Nutritional value of goat milk[J]. Food and Health,2001(4):19.
    [8]
    李静, 邓泽元, 范亚苇, 等. 几种乳制品中脂肪酸的特点[J]. 食品工业科技,2007,28(11):221−223. [LI J, DENG Z Y, FAN Y W, et al. Characteristics of fatty acids in several dairy products[J]. Food Industry Science and Technology,2007,28(11):221−223. doi: 10.3969/j.issn.1002-0306.2007.11.074
    [9]
    翁晨, 邓泽元, 李静. 乳制品脂质组成的比较[J]. 食品科学,2020,41(4):149−156. [WENG C, DENG Z Y, LI J. Comparison of lipid compositions of several dairy products[J]. Food Science,2020,41(4):149−156. doi: 10.7506/spkx1002-6630-20190403-046
    [10]
    WENG C, DENG Z Y, ZHANG N, et al. Lipid profiles of Chinese soft-shell turtle eggs(Pelodiscus sinensis)[J]. Journal of Food Composition and Analysis,2020,94:103627. doi: 10.1016/j.jfca.2020.103627
    [11]
    KALUZNY M A, DUNCAN L A, MERRITT M V, et al. Rapid separation of lipid classes in high yield and purity using bonded phase columns[J]. Journal of Lipid Research,1985,26(1):135−140. doi: 10.1016/S0022-2275(20)34412-6
    [12]
    LEI, LI J, LI G Y, et al. Stereospecific analysis of triacylglycerol and phospholipid fractions of five wild freshwater fish from poyang lake[J]. Journal of Agricultural and Food Chemistry,2012,60(7):1857−1857. doi: 10.1021/jf204584t
    [13]
    ICHIHARA K, SHIBAHARA A, YAMAMOTO K, et al. An improved method for rapid analysis of the fatty acids of glycerolipids[J]. Lipids,1996,31(5):535−539. doi: 10.1007/BF02522648
    [14]
    胡盛本, 卓成飞, 邹古月, 等. 氢化大豆油和反刍动物油脂中甘油三酯和磷脂反式脂肪酸的位置分布[J]. 食品科学,2017,38(14):276−283. [HU S B, ZHUO C F, ZOU G Y, et al. Positional distribution of trans fatty acids in triglycerides and phospholipids of partially hydrogenated soybean oil and ruminant animal fat[J]. Food Science,2017,38(14):276−283. doi: 10.7506/spkx1002-6630-201714043
    [15]
    CRISTINA C H, DENG Z, ZHOU J, et al. Methods for analysis of conjugated linoleic acids and trans-18: 1 isomers in dairy fats by using a combination of gas chromatography, silver-ion thin-layer chromatography/gas chromatography, and silver-ion liquid chromatography[J]. Journal of AOAC International,2004,87(2):545−562. doi: 10.1093/jaoac/87.2.545
    [16]
    吴琳, 刘四磊, 魏芳, 等. Florisil固相萃取法联用气相色谱测定油脂中Sn-2位脂肪酸[J]. 中国油料作物学报,2015,37(2):227−233. [WU L, LIU S L, WEI F, et al. Determination of Sn-2 position fatty acid in oils by SPE method with Florisil extraction cartridge coupled with gas chromatography[J]. Chinese Journal of Oil Crop Sciences,2015,37(2):227−233. doi: 10.7505/j.issn.1007-9084.2015.02.016
    [17]
    齐冬梅, 邹孝强, 黄健花, 等. DHA单细胞油脂的脂肪酸分布及甘油三酯组成分析[J]. 中国油脂,2018,43(9):161−167. [QI D M, ZOU X Q, HUANG J H, et al. Fatty acid distribution and triglyceride composition of DHA single cell oil[J]. China Oils and Fats,2018,43(9):161−167.
    [18]
    MARKO, TARVAINEN, HEIKKI, et al. Regiospecific analysis of triacylglycerols by ultrahigh-performance-liquid chromatography-electrospray ionization-tandem mass spectrometry[J]. Analytical Chemistry,2019,91(21):13695−13702. doi: 10.1021/acs.analchem.9b02968
    [19]
    SI W, LIANG Y, MA K Y, et al. Antioxidant activity of capsaicinoid in canola oil[J]. Journal of Agricultural and Food Chemistry,2012,60(24):6230−6234. doi: 10.1021/jf301744q
    [20]
    MURIEL M E, ANTEQUERA M T, PETRON M J, et al. Stereospecific analysis of fresh and dry-cured muscle phospholipids from Iberian pigs[J]. Food Chemistry,2005,90(3):437−443. doi: 10.1016/j.foodchem.2004.05.007
    [21]
    ROACH J, MOSSOBA M M, YURAWECZ M P, et al. Chromatographic separation and identification of conjugated linoleic acid isomers[J]. Analytica Chimica Acta,2002,465(1):207−226.
    [22]
    MARCEL B, BRIGITTE F, CLAUDLA C, et al. Quantification of phospholipids in infant formula and growing up milk by high-performance liquid chromatography with evaporative light scattering detector[J]. Journal of AOAC International,2010,93(3):948−955. doi: 10.1093/jaoac/93.3.948
    [23]
    FIDLER N, KOLETZKO B. The fatty acid composition of human colostrum[J]. European Journal of Nutrition,2000,39(1):31−37. doi: 10.1007/s003940050073
    [24]
    卓成飞, 胡盛本, 邓泽元, 等. 液态乳中胆固醇、7-脱氢胆固醇及25-羟基胆固醇的同步测定方法[J]. 中国食品学报,2019,19(4):232−240. [ZHUO C F, HU S B, DENG Z Y, et al. Synchronous determination method of cholesterol, 7-dehydrocholesterol and 25-hydroxycholesterol in liquid milk[J]. Journal of Chinese Institute of Food Science and Technology,2019,19(4):232−240.
    [25]
    BAUER L C, SANTANA D, MACEDO M, et al. Method validation for simultaneous determination of cholesterol and cholesterol oxides in milk by RP-HPLC-DAD[J]. Journal of the Brazilian Chemical Society,2014,25(1):161−168.
    [26]
    SHEILA M, INNIS. Dietary (n-3) fatty acids and brain development[J]. The Journal of Nutrition,2007,137(4):855−859. doi: 10.1093/jn/137.4.855
    [27]
    杨普煜, 张虹, 刘浚辰, 等. Sn- 2棕榈酸酯对婴幼儿健康的促进作用[J]. 食品科学技术学报,2018,36(4):41−45. [YANG P Y, ZHANG H, LIU J C, et al. Review of Sn-2 palmitate implications for infant health[J]. Journal of Food Science and Technology,2018,36(4):41−45. doi: 10.3969/j.issn.2095-6002.2018.04.006
    [28]
    ST-ONGE M P, ROSS R, PARSONS W D, et al. Medium-chain triglycerides increase energy expenditure and decrease adiposity in overweight men[J]. Obesity,2012,11(3):395−402.
    [29]
    张星弛, 韩培涛, 李晓莉, 等. 中链甘油三酯的研究进展[J]. 食品研究与开发,2017,38(23):220−224. [ZHANG X C, HAN P T, LI X L, et al. Research progress of medium chain triglycerides[J]. Food Research and Development,2017,38(23):220−224. doi: 10.3969/j.issn.1005-6521.2017.23.042
    [30]
    徐文迪. 富含中长碳链甘油三酯的人乳替代脂的酶法制备[D]. 无锡: 江南大学, 2019.

    XU W D. Enzymatic preparation of human milk substitute fat rich in medium and long chain triglycerides[D]. Wuxi: Jiangnan University, 2019.

Catalog

    Article Metrics

    Article views (289) PDF downloads (20) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return