Screening of Selenium-tolerant <i>Bacillus </i>and Exploration of Its Selenite Reduction Mechanism

YANG Rui; YU Yonghe; CHENG Shuiyuan; WANG Zhangqian

doi:10.13386/j.issn1002-0306.2021020149

Volume 42 Issue 22

Nov. 2021

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Science and Technology of Food Industry > 2021 > 42(22): 105-111. > DOI: 10.13386/j.issn1002-0306.2021020149

YANG Rui, YU Yonghe, CHENG Shuiyuan, et al. Screening of Selenium-tolerant Bacillus and Exploration of Its Selenite Reduction Mechanism[J]. Science and Technology of Food Industry, 2021, 42(22): 105−111. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021020149.

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Screening of Selenium-tolerant Bacillus and Exploration of Its Selenite Reduction Mechanism

YANG Rui^1,,
YU Yonghe¹,
CHENG Shuiyuan^{1, 2, 3, ,},
WANG Zhangqian^{1, 2, 3, ,}

1.
School of Life Science and Technology, Wuhan Polytechnic University, Wuhan 430023, China
2.
National R & D Center for Se-Rich Agricultural Products Processing, Wuhan Polytechnic University, Wuhan 430023, China
3.
School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China

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Received Date: February 22, 2021
Available Online: September 13, 2021

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Abstract

Abstract

Microbes can convert oxidized toxic selenium into non-toxic selenium nanoparticles(SeNPs), this "green synthesis" method has great application potential in functional product development, environmental governance, and medical treatment. In this study, strain F4 was isolated from soybean planting bases with long-term application of selenium fertilizer. It was tested for selenium tolerance, identification classification, growth reduction kinetic analysis. In order to understand the mechanism of selenite reduction and elemental selenium formation, as well as its intracellular and extracellular distribution, the location of the selenite reduction reaction in bacterial culture were studied by in vitro reduction localization experiments. The results showed that, the strain F4 belonged to Bacillus altitudinis. When the culture was supplemented of 50 mmoL/L sodium selenite, the bacterial colony survival rate was 37.08%, and the tolerance to sodium selenite was as high as 250 mmoL/L. In the selenium-containing medium, the strain grew then reduced sodium selenite in the middle and late logarithmic phase, and produces a red precipitate in the stable phase. After incubating the reaction system for the in vitro reduction localization test at 33 ℃ for 24 h, reactivity was observed in the culture supernatant and extracellular polysaccharide, and the cytoplasm changed slightly with the addition of NADH. This bacillus could be used as a strain for producing red nano-selenium. It could provide a reference for the biotransformation of selenium.
- Bacillus altitudinis,
- selenium nanoparticles(SeNPs),
- selenite reduction,
- intracellular localization

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