Composition Analysis of <i>Urechis unicinctus</i> Body Wall Polysaccharide and Its Enrichment and Adsorption Based on MCM-41

MA Yueyun; MA Jingying; TANG Shiying; LIU Zonghao; ZUO Yijin; WANG Qiukuan; WU Long; ZHOU Hui

doi:10.13386/j.issn1002-0306.2021010256

Volume 42 Issue 19

Sep. 2021

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2021 > 42(19): 84-91. > DOI: 10.13386/j.issn1002-0306.2021010256

MA Yueyun, MA Jingying, TANG Shiying, et al. Composition Analysis of Urechis unicinctus Body Wall Polysaccharide and Its Enrichment and Adsorption Based on MCM-41[J]. Science and Technology of Food Industry, 2021, 42(19): 84−91. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021010256.

Citation:

PDF (2727 KB)

Composition Analysis of Urechis unicinctus Body Wall Polysaccharide and Its Enrichment and Adsorption Based on MCM-41

MA Yueyun^1,,
MA Jingying¹,
TANG Shiying¹,
LIU Zonghao¹,
ZUO Yijin¹,
WANG Qiukuan^{1, 2, 3},
WU Long^{1, 2, 3},
ZHOU Hui^{1, 2, 3, ,}

1.
College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China
2.
National R&D Branch Center for Seaweed Processing, Dalian 116023, China
3.
Key Laboratory of Aquatic Product Processing and Utilization of Liaoning Province, Dalian 116023, China

More Information

Received Date: January 31, 2021
Available Online: August 10, 2021

Graphical Abstract

Abstract

Abstract

In this paper, the polysaccharides from the body wall of Urechis unicinctus was extracted by pepsin, and the composition of the extracted polysaccharides were analyzed. The adsorption of polysaccharides on MCM-41 was studied by the adsorption kinetics, adsorption thermodynamics. The results of composition analysis showed that the content of the total sugar was (78.82%±1.4%), the content of total protein was (10.5%±1.07%), and the content of sulfate radical was (0.52%±0.06%). The monosaccharide mainly contained glucose, mannose, galactose and xylose. The results of infrared spectrum analysis showed that the polysaccharide contained characteristic groups such as O-H, C-O-C, C=O, S=O, which indicated that there were uronic acid in the polysaccharide, and there was substitution of sulfate group. The mesoporous material MCM-41, with the average pore diameter 3.85 nm and the pore volume 1.25 cm³/g, was used to enrich the polysaccharide. It was found that the adsorption kinetics of the polysaccharide on MCM-41 conformed to the pseudo second order adsorption kinetics (PSO) model, in which the external diffusion and intra particle diffusion controlled the adsorption process. The adsorption thermodynamics of the polysaccharide on MCM-41 was consistent with both the Langmuir model and Freundlich model, indicating that the adsorption process of MCM-41 was mainly monolayer and accompanied by the adsorption of multi-layer. It was found that 10% SDS had stronger elution ability, which was caused by the destruction of hydrogen bond interaction between polysaccharide and water. The study on the adsorption mechanism of polysaccharides from the Urechis unicinctus on mesoporous material MCM-41 is expected to provide theoretical guidance for the enrichment and separation of polysaccharides, oligosaccharides, glycoproteins and glycopeptides in food industry.
- Urechis unicyclicus,
- polysaccharide,
- MCM-41,
- mesoporous material,
- adsorption enrichment

FullText(HTML)

References (39)

References

[1]	Ma X, Liu X, Zhou D, et al. The NF-κB pathway participates in the response to sulfide stress in Urechis unicinctus[J]. Fish & Shellfish Immunology,2016,58:229−238.
[2]	Liu Xiaolong, Qin Zhenkui, Li Xueyu, et al. NF1, Sp1 and HSF1 are synergistically involved in sulfide-induced sqr activation in echiuran worm Urechis unicinctus[J]. Aquatic Toxicology,2016,175:232−240. doi: 10.1016/j.aquatox.2016.04.002
[3]	Li Xueyu, Liu Xiaolong, Qin Zhenkui, et al. A novel transcription factor Rwdd1 and its SUMO ylation inhibit the expression of sqr, a key gene of mitochondrial sulfide metabolism in Urechis unicinctus[J]. Aquatic Toxicology,2018,204:180−189. doi: 10.1016/j.aquatox.2018.09.012
[4]	Zhang Yongjun, Zhang Xiaoshuan, Mai Thi Tuyet Nga, et al. Development and evaluation of key ambient factors online monitoring system in live Urechis unicinctus transportation strategies[J]. Computers and Electronics in Agriculture,2018,145:43−52. doi: 10.1016/j.compag.2017.12.017
[5]	刘韵, 吴浩浩, 曾名湧. 不同水煮时间对单环刺汤汁调味基料营养和呈味成分的影响[J]. 食品工业科技,2017,38(4):267−271. [Liu Yun, Wu Haohao, Zeng Mingyong. Effect of different boiling time on the nutrition and taste components of the seasoning base of single ring spiny soup[J]. Food Industry Technology,2017,38(4):267−271.
[6]	刘志娟, 张朝辉, 赵雪, 等. 单环刺螠体壁胶原蛋白的提取及其理化性质[J]. 食品科学,2012,33(7):37−40. [Liu Zhijuan, Zhang Zhaohun, Zhao Xue, et al. Extraction and physicochemical properties of collagen from the body wall of the monocyclic thorn beetle[J]. Food Science,2012,33(7):37−40.
[7]	张晓晓, 刘峰, 刘春娥, 等. 响应面法优化酶解单环刺螠内脏多肽的工艺研究[J]. 食品研究与开发,2018,39(19):52−57. [Zhang Xiaoxiao, Liu Feng, Liu Chune, et al. Optimization of enzymatic hydrolysis of visceral peptides from Echinochloa japonicus by response surface methodology[J]. Food Research and Development,2018,39(19):52−57. doi: 10.3969/j.issn.1005-6521.2018.19.010
[8]	孙雪燕, 宋鸿旭, 任宇豪, 等. 单环刺螠纤溶酶ufeII基因的原核表达及活性研究[J]. 中国科技论文,2016,11(24):2797−2800, 2815. [Sun Xueyan, Song Hongxu, Ren Yuhao, et al. Study on prokaryotic expression and activity of fibrinolytic enzyme ufeII gene[J]. China Science and Technology Papers,2016,11(24):2797−2800, 2815. doi: 10.3969/j.issn.2095-2783.2016.24.009
[9]	韩宝芹, 杜芳, 毕庆庆, 等. 单环刺螠纤溶酶Ⅲ基因克隆及原核表达[J]. 中国海洋大学学报(自然科学版),2014,44(3):44−49. [Han Baoqin, Du Fang, Bi Qingqing, et al. Cloning and prokaryotic expression of fibrinolytic enzyme Ⅲ gene of echinochloa Chinensis[J]. Journal of Ocean University of China (Natural Science Edition),2014,44(3):44−49.
[10]	Qingqing Bi, Baoqin Han, Yilin Feng, et al. Antithrombotic effects of a newly purified fibrinolytic protease from Urechis unicinctus[J]. Thrombosis Research,2013,132(2):e135−e144. doi: 10.1016/j.thromres.2013.07.001
[11]	尹哲, 龙莎, 张嘉颖, 等. 单环刺螠不同部位中纤溶酶的分离纯化及其活性差异[J]. 华侨大学学报(自然科学版),2020,41(1):84−89. [Yin Zhe, Long Sha, Zhang Jiaying, et al. Separation and purification of fibrinolytic enzyme from different parts of E. monocylindricus and its activity difference[J]. Journal of Huaqiao University (Natural Science Edition),2020,41(1):84−89.
[12]	李倩倩, 刘双双, 陈海华, 等. 单环刺螠内脏蛋白酶的分离纯化与酶学性质研究[J]. 中国食品学报,2016,16(6):88−95. [Li Qianqian, Liu Shangshuang, Chen Haihua, et al. Isolation, purification and enzymatic properties of visceral protease from Echinochloa japonicus[J]. Chinese Journal of Food Science,2016,16(6):88−95.
[13]	刘双双, 王雨生, 陈海华. 单环刺螠内脏粗蛋白酶的提取工艺及酶学性质[J]. 中国食品学报,2014,14(6):48−55. [Liu Shuangshuang, Wang Yusheng, Chen Haihua. Extraction technology and enzymatic properties of crude protease from the viscera of Echinochloa japonicus[J]. Chinese Journal of Food Science,2014,14(6):48−55.
[14]	Ozeki Y, Tazawa E, Matsui T. D-galactoside-specific lectins from the body wall of an echiuroid (Urechis unicinctus) and two annelids (Neanthes japonica and Marphysa sanguinea)[J]. Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology,1997,118(1):1−6.
[15]	N G Busarova, S V Isai, I Yu Revtsova. Lipids from the marine worm Urechis unicinctus[J]. Chemistry of Natural Compounds,2011,47(4):515−518. doi: 10.1007/s10600-011-9984-3
[16]	E Ya Kostetsky, P V Velansky. Phospholipids of sea worms, mollusks, and arthropods[J]. Russian Journal of Marine Biology,2009,35(3):187−199. doi: 10.1134/S1063074009030018
[17]	Yuan Chunying, Liu Ping, Han Xu, et al. Hypoglycemic effects of glycosaminoglycan from Urechis unicinctus in diabetic mice[J]. Journal of medicinal food,2015,18(2):190−194. doi: 10.1089/jmf.2013.3139
[18]	朱森君, 陈米娜, 牛庆凤, 等. 单环刺螠内脏多糖结构的分析及其对脂质过氧化物的清除作用[J]. 食品科学,2015,36(5):67−71. [Zhu Senjun, Chen Mina, Niu Qingfeng, et al. Analysis of the structure of polysaccharides from the viscera of E. monocylindricus and its scavenging effect on lipid peroxides[J]. Food Science,2015,36(5):67−71. doi: 10.7506/spkx1002-6630-201505013
[19]	朱佳利, 陈依莎, 牛庆凤, 等. 单环刺螠体壁多糖的分离纯化、理化性质及抗脂质过氧化活性[J]. 食品科学,2015,36(8):162−166. [Zhu jiali, Chen yisha, Niu qinfeng, et al. Isolation, purification, physicochemical properties and anti-lipid peroxidation activity of polysaccharides from the body wall of Echinochloa japonica[J]. Food Science,2015,36(8):162−166. doi: 10.7506/spkx1002-6630-201508029
[20]	Wang Yaping, Shi Tiantian, Huang Guoqiang, et al. Molecular detection of eukaryotic diets and gut mycobiomes in two marine sediment-dwelling worms, Sipunculus nudus and Urechis unicinctus[J]. Microbes and Environments,2018,33(3):290−300. doi: 10.1264/jsme2.ME18065
[21]	焦绪栋, 安传锋, 王福亮, 等. 单环刺螠废弃内脏中粗多糖的提取工艺优化[J]. 食品科学,2013,34(2):27−30. [Jiao Dongxu, An Chuanfeng, Wang Fuliang, et al. Optimization of the extraction process of crude polysaccharides from waste viscera of Echinochloa japonicus[J]. Food Science,2013,34(2):27−30.
[22]	袁春营, 刘萍, 韩旭, 等. 单环刺螠糖胺聚糖组成结构与功能活性研究[J]. 食品工业,2014,35(8):181−183. [Yuan Chunying, Liu Ping, Han Xu, et al. Study on the composition, structure and functional activity of glycosaminoglycan from monocylindrica[J]. Food Industry,2014,35(8):181−183.
[23]	徐如人, 庞文琴, 霍启升. 分子筛与多孔材料化学[M]. 第二版. 北京: 科学出版社, 2020. Xu Ruren, Pang Wenqin, Huo Qisheng. Molecular sieve and porous material chemistry[M]. Second Edition. Beijing: Science Press, 2020.
[24]	Roik Nadiia V, Belyakova Lyudmila A, Dziazko Marina O. Selective sorptive removal of Methyl Red from individual and binary component solutions by mesoporous organosilicas of MCM-41 type[J]. Journal of Environmental Sciences,2021,99:59−71. doi: 10.1016/j.jes.2020.04.027
[25]	Héctor Martínez Pérez-Cejuela, Isabel Ten-Doménech, Jamal Haskouri, et al. Solid-phase extraction of phospholipids using mesoporous silica nanoparticles: Application to human milk samples[J]. Analytical and Bioanalytical Chemistry,2018,410(20):4847−4854. doi: 10.1007/s00216-018-1121-8
[26]	Jiang Honghong, Zhang Wendan, Yang Jianxi, et al. Miniaturized solid-phase extraction using a mesoporous molecular sieve SBA-15 as sorbent for the determination of triterpenoid saponins from Pulsatilla chinensis by ultrahigh-performance liquid chromatography-charged aerosol detection[J]. Journal of Pharmaceutical and Biomedical Analysis,2020(prepublish):113810.
[27]	张临松, 高万萍, 宋悦凡, 等. 裙带菜孢子叶褐藻聚糖硫酸酯分离树脂的筛选及抗氧化活性研究[J]. 大连海洋大学学报,2019,34(5):697−703. [Zhang Linsong, Gao Wanping, Song Yuefan, et al. Screening of undaria pinnatifida spore leaf fucoidan sulfate separation resin and its antioxidant activity[J]. Journal of Dalian Ocean University,2019,34(5):697−703.
[28]	Liu Weifeng, Liu Xuguang, Yang Yongzhen, et al. Selective removal of benzothiophene and dibenzothiophene from gasoline using double-template molecularly imprinted polymers on the surface of carbon microspheres[J]. Fuel,2014,117:184−190. doi: 10.1016/j.fuel.2013.09.031
[29]	Dai Chaomeng, Zhang Juan, Zhang Yalei, et al. Selective removal of acidic pharmaceuticals from contaminated lake water using multi-templates molecularly imprinted polymer[J]. Chemical Engineering Journal,2012,211-212:302−309. doi: 10.1016/j.cej.2012.09.090
[30]	Wu Y, Yan M, Cui J, et al. A multiple-functional Ag/SiO₂/Organic based biomimetic nanocomposite membrane for high-stability protein recognition and cell adhesion/detachment[J]. Advanced Functional Materials 2015, 25(36): 5823−5832.
[31]	Liu Shucheng, Pan Jianming, Zhu Hengjia, et al. Graphene oxide based molecularly imprinted polymers with double recognition abilities: The combination of covalent boronic acid and traditional non-covalent monomers[J]. Chemical Engineering Journal,2016,290:220−231. doi: 10.1016/j.cej.2016.01.061
[32]	M Monier, Amr AIbrahim, M M Metwally, et al. Surface ion-imprinted amino-functionalized cellulosic cotton fibers for selective extraction of Cu(II) ions[J]. International Journal of Biological Macromolecules,2015,81:736−746. doi: 10.1016/j.ijbiomac.2015.08.004
[33]	杨玉品. 单环刺螠(Urechis unicinctus)多糖的分离纯化和结构研究[D]. 青岛: 中国海洋大学, 2011. Yang Yupin, Isolation, purification and structure study of polysaccharides from monocyclic echinochloa[D]. Qingdao: Ocean University of China, 2011.
[34]	Fernando I P Shanura, Sanjeewa K K Asanka, Samarakoon Kalpa W, et al. FTIR characterization and antioxidant activity of water soluble crude polysaccharides of Sri Lankan marine algae[J]. ALGAE,2017,32(1):75−86. doi: 10.4490/algae.2017.32.12.1
[35]	辛勤, 罗孟飞, 现代催化研究方法[M]. 北京: 科学出版社, 2016. Xin Qin, Luo Mengfei. Modern catalytic research methods[M]. Beijing: Science Press, 2016.
[36]	Jung Weon Choi, Hee Jin Kim, Hayeon Ryu, et al. Three-dimensional double-network hydrogels of graphene oxide, alginate, and polyacrylonitrile for copper removal from aqueous solution[J]. Environmental Engineering Research,2020,25(6):924−929.
[37]	Sharaf Ahmed, Liu Yang. Mechanisms and kinetics of greywater treatment using biologically active granular activated carbon[J]. Chemosphere,2021,263:128113. doi: 10.1016/j.chemosphere.2020.128113
[38]	陈瑞环, 刘云, 张澜, 等. 高吸附量有机聚合物的合成及其对亚甲基蓝吸附性能研究[J]. 环境科学学报,2020,40(12):4297−4305. [Chen Ruihuan, Liu Yun, Zhang Lan, et al. Synthesis of organic polymer with high adsorption capacity and its adsorption performance for methylene blue[J]. Journal of Environmental Science,2020,40(12):4297−4305.
[39]	张洪坤. 大孔树脂吸附纯化茯苓多糖工艺研究[J]. 食品研究与开发,2017,38(23):67−71. [Zhang Hongkun. Study on adsorption and purification of Poria polysaccharides by macroporous resin[J]. Food Research and Development,2017,38(23):67−71. doi: 10.3969/j.issn.1005-6521.2017.23.012