Study on the Nutritional Ingredients and the Effect on the Repair of Damaged Skin Barrier Function of Donkey Bone Collagen

FAN Yumei; SHI Chuanchao; TIE Hang; ZHANG Xiaohan; ZHAO Haiqing; LIAO Feng

doi:10.13386/j.issn1002-0306.2021010211

Volume 42 Issue 23

Nov. 2021

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Science and Technology of Food Industry > 2021 > 42(23): 341-349. > DOI: 10.13386/j.issn1002-0306.2021010211

FAN Yumei, SHI Chuanchao, TIE Hang, et al. Study on the Nutritional Ingredients and the Effect on the Repair of Damaged Skin Barrier Function of Donkey Bone Collagen[J]. Science and Technology of Food Industry, 2021, 42(23): 341−349. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021010211.

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Study on the Nutritional Ingredients and the Effect on the Repair of Damaged Skin Barrier Function of Donkey Bone Collagen

1.
National Engineering Research Center for Gelatin-based Traditional Chinese Medicine, Dong-E-E-Jiao Co., Ltd., Liaocheng 252201, China
2.
Chinese Academy of Inspection and Quarantine, Beijing 100176, China

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Received Date: January 26, 2021
Available Online: September 24, 2021

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Abstract

Abstract

In order to explore the functional components of donkey bone collagen and the repair effect of donkey bone collagen on the damaged skin barrier. The functional composition of donkey bone collagen was analyzed by UPLC-Orbitrap MS technique, the effects of donkey bone collagen on the proliferation of HaCaT cells were detected by Brdu method, and the effects on the migration of HaCaT cells were detected by scratch method. The results showed that the protein content of donkey bone collagen was 57.5%, which had a typical collagen amino acid composition. The donkey bone collagen contained abundant functional ingredients such as amino acids, vitamins, sugar and lipids. Donkey bone collagen could significantly promote the proliferation and migration of HaCaT cells, and the effect of 0.15 g/mL donkey bone collagen in promoting the proliferation and migration of HaCaT cells was similar to that of 5% fetal bovine serum, which was significantly better than the blank control group (P<0.05). It is inferred that donkey bone collagen could accelerate the repair of skin barrier, and its mechanism might be related to promoting keratinocytes proliferation and migration.
- donkey bone collagen,
- nutritional ingredients,
- repair,
- HaCaT cells,
- skin barrier function

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References

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