LI Ren, JIANG Peng, DAI Lingyan, et al. Effects of Microwave Treatment on Physicochemical Properties and Protein Structure Characteristics of Sorghum[J]. Science and Technology of Food Industry, 2021, 42(23): 27−33. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021010203.
Citation: LI Ren, JIANG Peng, DAI Lingyan, et al. Effects of Microwave Treatment on Physicochemical Properties and Protein Structure Characteristics of Sorghum[J]. Science and Technology of Food Industry, 2021, 42(23): 27−33. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021010203.

Effects of Microwave Treatment on Physicochemical Properties and Protein Structure Characteristics of Sorghum

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  • Received Date: January 24, 2021
  • Available Online: September 25, 2021
  • In order to improve the physicochemical properties of sorghum protein, three kinds of sorghum were treated with microwave technology, and the changes of physicochemical properties and protein structural properties of sorghums before and after microwave treatment were measured by scanning electron microscopy and Fourier transform infrared spectrometer. The results showed that microwave treatment had significant effects on the physicochemical and protein structural properties of sorghum. Microwave treatment reduced the grain size of sorghum protein and caused cracks on the protein surface. However, the infrared spectra of sorghum protein before and after treatment had the similar structural characteristics and belonged to physical denaturation. Microwave treatment significantly increased the total protein contents of the three sorghums species by 0.6% (P<0.05), decreased soluble protein contents by 0.97~1.87 mg/g, and significantly decreased in vitro digestibility by 15.55%~43.63% (P<0.05). In addition, after microwave treatment, the water holding capacity of sorghum powder was significantly increased by 0.32~0.40 g/g (P<0.05), the oil holding capacity was significantly decreased by 0.08~0.18 g/g (P<0.05), the total number of bacterial colonies and the number of molds on the surface were significantly decreased by 98.75% and 98.94% (P<0.05), respectively. And the color darkened. The present research can present reference for the development and application of sorghum food.
  • [1]
    KAUR K D, JHA A, SABIKHI L, et al. Significance of coarse cereals in health and nutrition: A review[J]. Journal of Food Science and Technology-Mysore,2014,51(8):1429−1441. doi: 10.1007/s13197-011-0612-9
    [2]
    BELTON P S, DELGADILLO I, HALFORD N G, et al. Kafirin structure and functionality[J]. Journal of Cereal Science,2006,44(3):272−286. doi: 10.1016/j.jcs.2006.05.004
    [3]
    TAYLOR J R N, BELTON P S, BETA T, et al. Increasing the utilisation of sorghum, millets and pseudocereals: Developments in the science of their phenolic phytochemicals, biofortification and protein functionality[J]. Journal of Cereal Science,2014,59(3):257−275. doi: 10.1016/j.jcs.2013.10.009
    [4]
    王章存, 康艳玲. 国内外谷物蛋白发展概况[J]. 中国食品添加剂,2006,5:110−113. [WANG Z C, KANG Y L. Status of development on cereal protein[J]. China Food Additives,2006,5:110−113. doi: 10.3969/j.issn.1006-2513.2006.01.025
    [5]
    STEPHEN G G, RANIL C, DEAN B, et al. Physicochemical and antimicrobial properties of citral and quercetin incorporated kafirin-based bioactive films[J]. Food Chemistry,2015,168:341−347. doi: 10.1016/j.foodchem.2014.07.077
    [6]
    戴凌燕, 蔡欣月, 陈卓, 等. 高粱醇溶蛋白的研究进展[J]. 食品工业,2018,39(3):253−257. [DAI L Y, CAI X Y, CHEN Z, et al. Advance research of sorghum kafirin[J]. Food Industry,2018,39(3):253−257.
    [7]
    霍金杰, 肖志刚, 王娜, 等. 青稞蛋白质的微波辅助提取工艺及性质研究[J]. 食品研究与开发,2020,41(21):145−153. [HUO J J, XIAO Z G, WANG N, et al. Study on the microwave-assisted extraction technology and properties of barley protein[J]. Food Research and Development,2020,41(21):145−153.
    [8]
    刘海波, 廖超, 郑万琴, 等. 微波处理小麦面粉对淀粉及蛋白性质的影响[J]. 食品与发酵工业,2020,46(14):91−97. [LIU H B, LIAO C, ZHENG W Q, et al. Effect of microwave-treated wheat flour on starch and protein properties[J]. Food and Fermentation Industries,2020,46(14):91−97.
    [9]
    胡方洋, 陈金玉, 王轻, 等. 不同干燥方式对苦荞蛋白功能性质的影响[J]. 食品科技,2020,45(1):103−108. [HU F Y, CHEN J Y, WANG Q, et al. Effects of different drying methods on functional properties of tartary buckwheat protein[J]. Food Science and Technology,2020,45(1):103−108.
    [10]
    王娜, 孟利军, 黄忠民, 等. 加工方式对非发酵面团小麦醇溶蛋白致敏性的影响[J]. 农业工程学报,2020,36(9):292−299. [WANG N, MENG L J, HUANG Z M, et al. Effects of different processing on allergenicity of wheat gliadin in non-fermented dough[J]. Transactions of the Chinese Society of Agricultural Engineering,2020,36(9):292−299. doi: 10.11975/j.issn.1002-6819.2020.09.033
    [11]
    姜鹏, 李忍, 戴凌燕, 等. 浸泡和微波处理对三种高粱熟化的影响[J]. 食品工业科技,2021,42(8):70−74. [JIANG P, LI R, DAI L Y, et al. Effects of soaking and microwave treatments on cultivability of three kinds of sorghums[J]. Science and Technology of Food Industry,2021,42(8):70−74.
    [12]
    周剑敏, 尹方平, 于晨, 等. 高粱碱溶蛋白ACE抑制肽的制备及其稳定性研究[J]. 中国粮油学报,2019,34(8):66−72. [ZHOU J M, YIN F P, YU C, et al. Preparation and stability of ACE inhibitory peptides derived from sorghum protein[J]. Journal of the Chinese Cereals and Oils Association,2019,34(8):66−72. doi: 10.3969/j.issn.1003-0174.2019.08.012
    [13]
    赵志浩, 刘磊, 张名位, 等. 预酶解-挤压膨化对全谷物糙米粉品质特性的影响[J]. 食品科学,2019,40(1):108−116. [ZHAO Z H, LIU L, ZHANG M W, et al. Combined effect of enzymatic pretreatment and extrusion on quality properties of brown rice flour[J]. Food Science,2019,40(1):108−116. doi: 10.7506/spkx1002-6630-20171210-121
    [14]
    MERTZ E T, HASSEN M M, CAIRNS Whittern C, et al. Pepsin digestibility of proteins in sorghum and other major cereals[J]. Proceedings of the National Academy of Sciences of the United States of America,1984,81(1):1−2. doi: 10.1073/pnas.81.1.1
    [15]
    LIU H, FAN H, CAO R, et al. Physicochemical properties and in vitro digestibility of sorghum starch altered by high hydrostatic pressure[J]. International Journal of Biological Macromolecules,2016,92:753−760. doi: 10.1016/j.ijbiomac.2016.07.088
    [16]
    刘佳男, 于雷, 王婷, 等. 微波处理对白高粱淀粉理化特性的影响[J]. 食品科学,2017,38(5):186−190. [LIU J N, YU L, WANG T, et al. Effect of microwave treatment on physicochemical properties of white sorghum starch[J]. Food Science,2017,38(5):186−190. doi: 10.7506/spkx1002-6630-201705030
    [17]
    赵颖, 申莉丽, 姜雯翔, 等. 3种膨化方式处理对萌芽糙米品质的影响[J]. 食品与发酵工业,2016,42(3):150−155. [ZHAO Y, SHEN L L, JIANG W X, et al. Effects of three different puffing methods on the quality of germinated brown rice[J]. Food and Fermentation Industries,2016,42(3):150−155.
    [18]
    姜雯翔. 萌芽糙米热风微波干燥与焙炒及膨化加工研究[D]. 南京: 南京农业大学, 2014.

    JIANG W X. Study on hot air microwave drying, baking and expansion processing of sprout brown rice[D]. Nanjing: Nanjing Agricultural University, 2014.
    [19]
    王振宇, 刘欢, 马俪珍, 等. 热处理下的猪肉蛋白质特性[J]. 食品科学,2008,5:73−77. [WANG Z Y, LIU H, MA L Z, et al. Characteristics of porcine proteins by heat treatment[J]. Food Science,2008,5:73−77. doi: 10.3321/j.issn:1002-6630.2008.07.011
    [20]
    LI Y, HU A, ZHENG J, et al. Comparative studies on structure and physiochemical changes of millet starch under microwave and ultrasound at the same power[J]. International Journal of Biological Macromolecules,2019,141:76−84. doi: 10.1016/j.ijbiomac.2019.08.218
    [21]
    XU B, YUAN J, WANG L, et al. Effect of multi-frequency power ultrasound (MFPU) treatment on enzyme hydrolysis of casein[J]. Ultrasonics Sonochemistry,2020,63:104930. doi: 10.1016/j.ultsonch.2019.104930
    [22]
    SIANO F, SORRENTINO G, RICCARDI M, et al. Chemical, nutritional, and spectroscopic characterization of typical ecotypes of Mediterranean area beans[J]. European Food Research and Technology,2018,244(5):795−804. doi: 10.1007/s00217-017-3004-1
    [23]
    徐丽. 富含γ-氨基丁酸的小米发芽条件优化及特性分析[D]. 哈尔滨: 东北农业大学, 2019.

    XU L. Optimization of germinating conditions and characteristic analysis of millet rich in γ-aminobutyric acid[D]. Harbin: Northeast Agricultural University, 2019.
    [24]
    曾剑华, 孟妍, 刘琳琳, 等. 基于多光谱技术表征汉麻分离蛋白构象并分析pH对其构象的影响[J]. 光谱学与光谱分析,2020,40(12):3748−3754. [ZENG J H, MENG Y, LIU L L, et al. Characterization and effects of pH on the conformation of hemp protein isolate based on multi-spectroscopic technique[J]. Spectroscopy and Spectral Analysis,2020,40(12):3748−3754.
    [25]
    AWIKA J M, ROONEY L W. Sorghum phytochemicals and their potential impact on human health[J]. Phytochemistry,2004,65(9):1199−1221. doi: 10.1016/j.phytochem.2004.04.001
    [26]
    DUODU K G, NUNES A, DELGADILLO I, et al. Effect of grain structure and cooking on sorghum and maize in vitro protein digestibility[J]. Journal of Cereal Science,2002,35(2):161−174. doi: 10.1006/jcrs.2001.0411
    [27]
    ADEBIYI J A, OBADINA A O, MULABA Bafubiandi A F, et al. Effect of fermentation and malting on the microstructure and selected physicochemical properties of pearl millet (Pennisetum glaucum) flour and biscuit[J]. Journal of Cereal Science,2016,70:132−139. doi: 10.1016/j.jcs.2016.05.026
    [28]
    OBASI N E, UNAMMA N C, NWOFIA G E. Effect of dry heat pre-treatment (toasting) on the cooking time of cowpea (Vigna unguiculata L. Walp)[J]. Nigerian Food Journal,2014,32(2):16−24. doi: 10.1016/S0189-7241(15)30113-2
    [29]
    HU Z, SHAO Y, LU L, et al. Effect of germination and parboiling treatment on distribution of water molecular, physicochemical profiles and microstructure of rice[J]. Journal of Food Measurement and Characterization,2019,13(3):1898−1906. doi: 10.1007/s11694-019-00108-5
    [30]
    AUKSORNSRI T, BORNHORST E R, TANG J, et al. Developing model food systems with rice based products for microwave assisted thermal sterilization[J]. LWT- Food Science and Technology,2018,96:551−559. doi: 10.1016/j.lwt.2018.05.054
    [31]
    SRISANG N, VARANYANOND W, SOPONRONNARIT S, et al. Effects of heating media and operating conditions on drying kinetics and quality of germinated brown rice[J]. Journal of Food Engineering,2011,107(3):385−392.
    [32]
    AUKSORNSRI T, TANG J, TANG Z W, et al. Dielectric properties of rice model food systems relevant to microwave sterilization process[J]. Innovative Food Science & Emerging Technologies,2018,45:98−105.
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