Enhanced Detection of Uric Acid through Fe<sub>3</sub>O<sub>4</sub>@Au Based Highly Sensitive Electrochemical Biosensor

GUAN Huanan; WU Qiaoyan; PENG Bo; XUE Yue; GONG Dezhuang; LIU Xiaofei; ZHANG Na

doi:10.13386/j.issn1002-0306.2021010130

Volume 42 Issue 22

Nov. 2021

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2021 > 42(22): 254-260. > DOI: 10.13386/j.issn1002-0306.2021010130

GUAN Huanan, WU Qiaoyan, PENG Bo, et al. Enhanced Detection of Uric Acid through Fe₃O₄@Au Based Highly Sensitive Electrochemical Biosensor[J]. Science and Technology of Food Industry, 2021, 42(22): 254−260. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021010130.

Citation:

PDF (2675 KB)

Enhanced Detection of Uric Acid through Fe₃O₄@Au Based Highly Sensitive Electrochemical Biosensor

College of Food Engineering, Harbin University of Commerce, Harbin 150076, China

More Information

Received Date: January 17, 2021
Available Online: September 12, 2021

Graphical Abstract

Abstract

Abstract

In this paper, we reported an electrochemical biosensor based on Fe₃O₄@Au nanocomposite for the detection of uric acid (UA). The nanocomposite was prepared by self-assembling technique and the characterisation was carried out using transmission electron microscopy (TEM). A highly sensitive and selective enzyme-free electrochemical based on peroxidemimetic enzyme activity of composite nanoparticles was prepared for the detection uric acid. Cyclic voltammetry (CV) was used to assess the electrocatalytic response of Fe₃O₄@Au towards uric acid, and then the Fe₃O₄@Au exhibited high electrocatalytic response which was attributed to the increased surface area and conductivity of the nanocomposite. The results showed that Fe₃O₄ functionalized with amino groups could be effectively loaded with gold nanoparticles. The optimum detection conditions were as follows: Amount of temperature 60 ℃, Fe₃O₄@Au suspension 1.20 mg/mL, scanning rate was 0.1 V/s, and the electrolyte pH was 5.5. The novel electrochemical detection method showed an extremely high sensitivity towards uric acid under the optimized conditions, with a good linear relationship between the current response and the concentration of uric acid in the range of 0.1~10 mmol/L, and then the equation was y=13.267x+6.044 (R²=0.9952), with the corresponding limit of detection of 0.087 μmol/L. The recoveries ranged from 97.9%~110.2%%, recycling was very well and the accuracy of method was very high. The sensor showed excellent sensitivity for uric acid with good stability and selectivity. Thus, these results implied that the electrochemical sensor provided a popular price and simple method for the reliable analysis of uric acid.
- gold magnetic particle,
- mimetic enzyme,
- uric acid,
- electrochemical detection,
- analytical chemistry

FullText(HTML)

References (30)

References

[1]	DONG W Y, HU X J, CHEN Z C, et al. An innovative bio-tissue network signal amplifier activated by high-N-doped carbon for uric acid detection[J]. Materials Chemistry and Physics,2020:254.
[2]	WANG Q W, WEN X, KONG J M, et al. Recent progress on uric acid detection: A review[J]. Critical Reviews in Analytical Chemistry,2020,50(4):1−17.
[3]	PATIL ANIRUDDHA B, ZHENG CHUANBAO, MA LIYUN, et al. Flexible and disposable gold nanoparticles-N-doped carbon-modified electrochemical sensor for simultaneous detection of dopamine and uric acid[J]. Nanotechnology,2021,32(6):065502. doi: 10.1088/1361-6528/abc388
[4]	依力哈木·扎依尔, 杨艳伟, 朱英, 等. 自动固相萃取-气相色谱-质谱法测定生活饮用水中60种半挥发性有机物[J]. 环境卫生学杂志,2020,10(1):81−88. [YILIHAMU Z, YANG Y W, ZHU Y, et al. Determination of 60 semi-volatile organic compounds in drinking water using automatic solid phase extraction-gas chromatography-mass spectrometry[J]. Journal of Environmental Hygiene,2020,10(1):81−88.
[5]	ZHANG Y M, HUANG H P, XU L. A novel electrochemical sensor based on Au-Dy 2 (WO 4 3 nanocomposites for simultaneous determination of uric acid and nitrite[J]. Chinese Journal of Analytical Chemistry,2019,48(3):e20032−e20037.
[6]	WANG H Y, HUI Q S, XU L X, et al. Fluorimetric determination of dopamine inpharmaceutica products and urine using ethylene diamine as the fluorigenic reagent[J]. Anal Chim Acta,2020,497(1−2):93−99.
[7]	WANG X, LU J J, TANG X M, et al. Colorimetric detection of uric acid with high sensitivity using Cu₂O@Ag nanocomposites[J]. Chemistry Afric,2020,3:749−758. doi: 10.1007/s42250-020-00122-x
[8]	LI N I, CHEN Y T, HU Y P, et al. Mobile healthcare system based on the combination of a lateral flow pad and smartphone for rapid detection of uric acid in whole blood[J]. Biosensors & bioelectronics,2020:164.
[9]	何云清. 紫外分光光度法与磷钨酸还原法测定尿酸浓度对比研究[J]. 广东化工,2017,44(9):250−251. [HE Y Q. A comparative study on the measurement of uric acid using ultraviolet spectrophotometry and phospho-tungstic acid deoxidizing method[J]. Guangdong Chemical Industry,2017,44(9):250−251. doi: 10.3969/j.issn.1007-1865.2017.09.111
[10]	KURBANOGLU S, OZKAN S A, MERKOÇI A. Nanomaterials-based enzyme electrochemical biosensors operating through inhibition for biosensing applications[J]. Biosensors and Bioelectronics,2017,89(2):886−898.
[11]	MALLIKARJUNA K, VEERA M R Y, BATHINAPATLA S, et al. Simple synthesis of biogenic PdAg bimetallic nanostructures for an ultra-sensitive electrochemical sensor for sensitive determination of uric acid[J]. Journal of Electroanalytical Chemistry,2018,822:163−170. doi: 10.1016/j.jelechem.2018.05.019
[12]	PARVANEH R, YVONNE J. Enzyme-based biosensors for choline analysis: A review[J]. Trends in Analytical Chemistry,2018,110:367−374.
[13]	钟青梅, 黄欣虹, 覃庆敏, 等. 以碳量子点为过氧化物模拟酶的葡萄糖测定方法[J]. 分析化学,2018,46(7):1062−1068. [ZHONG Q M, HUANG X H, QIN Q M, et al. Determination of glucose based on carbon quantum dots as peroxidase mimetic enzyme[J]. Chinese Journal of Analytical Chemistry,2018,46(7):1062−1068. doi: 10.11895/j.issn.0253-3820.171396
[14]	CHENG H, WANG X, WEI H. Ratiometric electrochemical sensor for effective and reliable detection of ascorbic acid in living brains[J]. Analytical Chemistry,2015,87(17):8889−8895. doi: 10.1021/acs.analchem.5b02014
[15]	LIU J, ZHAO Z, DING Z, et al. Degradation of 4-chlorophenol in fentonr-like system using Au-Fe omagnetic nanocompositesas the heterogeneous catalyst at near neutral condition[J]. RSC Adv,2016(6):53080−53088.
[16]	王鸿, 刘旭挺, 黄园, 等. 抗生素功能化金磁纳米探针的制备及其过氧化物模拟酶性能研究[J]. 中国科学:化学,2019,49(2):391−398. [WANG H, LIU X T, HUANG Y, et al. Preparation of antibiotic functionalized gold-magnetic nanoprobes and the study of their mimic peroxidase properties[J]. Scientia Sinica (Chimica),2019,49(2):391−398.
[17]	李铸衡, 高晶清, 简明红, 等. 构建金纳米粒子标记的凝集素芯片应用于抗生素与金黄色葡萄球菌相互作用研究[J]. 分析化学,2018(12):1904−1912. [LI Z H, GAO J Q, JIAN M H, et al. Fabrication of lectin microarray for studying interactions of antibiotics with staphylococcus aureus by gold nanoparticle probes[J]. Chinese Journal of Analytical Chemistry,2018(12):1904−1912. doi: 10.11895/j.issn.0253-3820.181570
[18]	LEONEL ALICE G, MANSUR ALEXANDRA A P, MANSUR HERMAN S. Advanced functional nanostructures based on magnetic iron oxide nanomaterials for water remediation: A review[J]. Water Research, 2020, 190: 116693−116697.
[19]	王文姣, 庄钊, 白瑞钦, 等. 四氧化三铁(Fe₃O₄)磁性复合材料在废水处理中的研究进展[J]. 胶体与聚合物,2020,38(4):186−190. [WANG W J, ZHUANG Z, BAI R Q, et al. Research progress of ferroferric oxide (Fe₃O₄) magnetic composite materials in wastewater treatment[J]. Chinese Journal of Colloid & Polymer,2020,38(4):186−190.
[20]	TAVALLAIE R, CARROLL J M, GRAND M L, et al. Gooding, nucleic acid hybridization on an electrically reconfigurable network of gold-coated magnetic nanoparticles enables microRNA detection in blood[J]. Nature Nanotechnol,2018,13(38):1066−1071.
[21]	WANG W W, HAO C L, SUN M ZH, et al. Spiky Fe₃O₄@Au supraparticles for multimodal in vivo imaging[J]. Advanced Functional Materials,2018,28(22):1800310. doi: 10.1002/adfm.201800310
[22]	LIU Y M, YANG J J, CAO J T, et al. An electrochemiluminescence aptasensor based on CdSe/ZnS functionalized MoS and enzymatic biocatalytic precipitation for sensitive detection of immunoglobulin E[J]. Sensors and Actuators B Chemical,2016,232:538−544. doi: 10.1016/j.snb.2016.03.165
[23]	SHEN G Y, ZHANG S B, SHENG G L, et al. Development of an electrochemical aptasensor for thrombin based on aptamer/Pd-AuNPs/HRP conjugates[J]. Anal. Methods,2016,8(10):2150−2155.
[24]	AFSHARANN H, KHALILZADEH B, TAJALLI H, et al. A sandwich type immunosensor for ultrasensitive electrochemical quantification of p53 protein based on gold nanoparticles/graphene oxide[J]. Electrochimica Acta,2016,188:153−164. doi: 10.1016/j.electacta.2015.11.133
[25]	韩博林, 关桦楠. 金磁微粒的制备及其催化性能[J]. 食品工业科技,2017,38(20):1−5, 10. [HAN B L, GUAN H N. Preparation and catalytic performance of gold magnetic particles[J]. Science and Technology of Food Industry,2017,38(20):1−5, 10.
[26]	SUN D F. Fabrication of one-dimensional nanomaterials and their electrochemistry properties based on electrospinning[D]. Hebei: Yanshan University, 2011.
[27]	Oliveira G C M D, Carvalho J, Brazaca L C, et al. Flexible platinum electrodes as electrochemical sensor and immunosensor of Parkinson’s disease biomarkers[J]. Biosensors & Bioelectronics,2020,152(3):112016.
[28]	HUANG Y, XUE Y W, ZENG J X, et al. Non-enzymatic electrochemical hydrogen peroxide biosensor based on reduction graphene oxide-persimmon tannin-platinum nanocomposite[J]. Materials Science and Engineering:C,2018,92:590−598. doi: 10.1016/j.msec.2018.07.021
[29]	牛博怀, 王文廉. 活化碳布电化学传感器对尿酸的检测研究[J]. 分析科学学报,2020,36(3):400−404. [NIU B H, WANG W L. Detection of uric acid by activated carbon cloth electrochemical sensor[J]. Journal of Analytical Science,2020,36(3):400−404.
[30]	张英, 张智彦, 马华, 等. 电化学方法快速测定尿酸的应用研究[J]. 四川理工学院学报(自然科学版),2009,22(4):85−87. [ZHANG Y, ZHANG Z Y, MA H, et al. Application of electrochemical method for rapid determination of uric acid[J]. Journal of Sichuan University of Science & Engineering(Natural Science Edition),2009,22(4):85−87.