MA Zhiqiang, ZHONG Yan, WEI Xuelin, et al. Comparison of Pork Quality in Different Frozen Storage Time and Its Change Mechanism[J]. Science and Technology of Food Industry, 2021, 42(18): 48−56. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021010063.
Citation: MA Zhiqiang, ZHONG Yan, WEI Xuelin, et al. Comparison of Pork Quality in Different Frozen Storage Time and Its Change Mechanism[J]. Science and Technology of Food Industry, 2021, 42(18): 48−56. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021010063.

Comparison of Pork Quality in Different Frozen Storage Time and Its Change Mechanism

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  • Received Date: January 12, 2021
  • Available Online: July 12, 2021
  • In order to understand the possible reasons and mechanisms of pork quality changes in frozen storage, the quality indicators such as water holding capacity, texture and color were tested to pork for 4 months and 8 months of frozen storage. The type and distribution of moisture in pork samples were detected by low-field nuclear magnetic resonance (LF-NMR), and the correlation between different water distribution and water holding capacity, texture was explored. The changes in pork flavor components and the types of characteristic substances were discussed by the electronic nose and lipidomics analysis based on the time-of-flight mass spectrometer. The results showed that the quality of frozen pork for 8 months significantly deteriorated, and the morphology of the muscle fibers was looser, the water relaxation time was delayed, the peak area was reduced, compared with pork frozen for 4 months. The quality indicators such as water holding capacity and texture of pork were positively correlated with frozen storage time, and negatively correlated with moisture relaxation time and peak area, which also indicated that the main reason for the deterioration of pork texture after a long period of freezing storage might be including the migration and loss of bound water and non-flowing water in pork. The partial least square regression analysis (PLS-DA) of pork electronic nose data found that the flavor components of pork also changed significantly after long-term frozen storage. The changed flavor components were mainly alkanes, aromatic components, weakly polar compounds, methane, and alcohol compounds. The identification of five different compounds related to oxidation by high-resolution mass spectrometry indicated that the significant changes in pork flavor might be caused by the oxidation of lipids in muscle tissue after a long period of freezing storage.
  • [1]
    李芳菲, 王博, 石硕, 等. 低温胁迫肌原纤维蛋白结构和热稳定性的变化[J]. 中国食品学报,2019,19(3):232−241. [Li F F, Wang B, Wang S, et al. Pan nan change of protein structure and thermal stability of myofibrillar protein under cold stress[J]. Journal of Chinese Institute of Food Science and Technology,2019,19(3):232−241.
    [2]
    黄鸿兵, 徐幸莲, 周光宏. 冷冻贮藏对冻猪肉冰晶形态、TVB-N及TBARS的影响[J]. 食品工业科技,2008,2(2):117−119, 122. [Huang H B, Xu X L, Zhou G H. Effect of frozen storage on ice crystal, TVB-N and TBARS of pork muscle[J]. Science and Technology of Food Industry,2008,2(2):117−119, 122.
    [3]
    Gil M, Duma-Kocan P, Stanislawczyk R, et al. The effect of freezing storage on physical and chemical properties of wild boar meat[J]. Czech Journal of Food Sciences,2018,36(6):487−493.
    [4]
    Pomponio L, Ruiz-Carrascal J. Oxidative deterioration of pork during superchilling storage[J]. Journal of the Science of Food and Agriculture,2017,97(15):5211−5215. doi: 10.1002/jsfa.8403
    [5]
    夏秀芳, 孔保华. 冻藏温度和时间对猪肉品质影响的研究[C]//北京: 中国机械工程学会包装与食品工程分会, 2010.

    Xia X F, Kong B H. The effects of freezing temperature and time on qualities of pork[C]//Beijing: Proceedings of the 2010 Annual Conference of Packaging and Food Engineering Branch, Chinese Society of Mechanical Engineering, 2010.
    [6]
    李靖, 袁乙平, 刘婷, 等. 基于低场核磁共振技术的冷冻猪背最长肌品质变化研究[J]. 食品与机械,2019,35(5):149−155. [Li J, Yuan Y P, Liu T, et al. Research on the change of quality of pork longissimus dorsi during the frozen period based on low field nuclear magnetic resonance technology[J]. Food & Machinery,2019,35(5):149−155.
    [7]
    Kim H W, Miller D K, Yan F F, et al. Probiotic supplementation and fast freezing to improve quality attributes and oxidation stability of frozen chicken breast muscle[J]. Lwt-Food Science and Technology,2017,75(7):34−41.
    [8]
    Shen Q, Wang M, Tian J, et al. Effects of Chinese pickled and dried mustard on nutritional quality, sensory quality, and shelf life of steamed pork belly[J]. Food Science & Nutrition,2018,6(4):747−756.
    [9]
    胡春辉, 徐青, 孙璇, 等. 几种典型扫描电镜生物样本制备[J]. 湖北农业科学,2016,55(20):5389−5392, 5402. [Hu C H, Xu Q, Sun X, et al. Several biological typical samples preparation methods of scanning electron microscope[J]. Hubei Agricultural Sciences,2016,55(20):5389−5392, 5402.
    [10]
    常海军, 唐翠, 唐春红. 不同解冻方式对猪肉品质特性的影响[J]. 食品科学,2014,35(10):1−5. [Chang H J, Tang C, Tang C H. Effects of different thawing methods on pork quality[J]. Food Science,2014,35(10):1−5. doi: 10.7506/spkx1002-6630-201410001
    [11]
    Lakshmanan R, Piggott J R, Paterson A. Potential applications of high pressure for improvement in salmon quality[J]. Trends in Food Science & Technology,2003,14(9):354−363.
    [12]
    戴志远, 崔雁娜, 王宏海. 不同冻藏条件下养殖大黄鱼鱼肉质构变化的研究[J]. 食品与发酵工业,2008,34(8):188−191. [Dai Z Y, Cui Y N, Wang H H. Changes of textural properties of cultured pseudosciaena crocea muscle under different frozen storage conditions[J]. Food & Fermentation Industries,2008,34(8):188−191.
    [13]
    Hattula T, Elfving K, Mroueh T, et al. Use of liquid smoke flavouring as an alternative to traditional flue gas smoking of rainbow trout fillets (Oncorhynchus mykiss)[J]. Lwt-Food Science & Technology,2001,34(8):521−525.
    [14]
    Daszkiewicz T, Lipowski T, Kubiak, et al. Effect of freezer storage on quality of M. longissimus lumborum from fallow deer (Dama dama L.)[J] South African Journal of Animal Science, 2017, 6(47): 834-841.
    [15]
    Stanislawczyk R, Rudy M, Gil M. The influence of frozen storage and selected substances on the quality of horse meat[J]. Meat Science,2019,155(10):74−78.
    [16]
    Lv W Q, Zhang M, Bhandari B, et al. Smart NMR method of measurement of moisture content of vegetables during microwave vacuum drying[J]. Food and Bioprocess Technology,2017,10(12):2251−2260. doi: 10.1007/s11947-017-1991-3
    [17]
    Fundo J F, Amaro A L, Madureira A R, et al. Fresh-cut melon quality during storage: An NMR study of water transverse relaxation time[J]. Journal of Food Engineering,2015,167(8):71−76.
    [18]
    张驰, 阮征. 低场核磁共振(LF-NMR)及其成像技术(MRI)在食品应用中的研究进展[C]//广州: “健康中国2030·健康食品的安全与创新”学术研讨会暨2018年广东省食品学会年会, 2018: 119-125.

    Zhang C, Ruan Z. Research progress of low-field nuclear magnetic resonance research progress of low-field nuclear magnetic resonance[C]//Guangzhou: “Healthy China 2030: Safety and Innovation of Healthy Food” Academic Seminar and 2018 Annual Meeting of Guangdong Food Society, 2018: 119−125.
    [19]
    姜晓文, 韩剑众. 生鲜肉品持水性的核磁共振研究[J]. 食品工业科技,2009,98(1):322−325. [Jiang X W, Han J Z. Research progress of water-holding capacity of fresh meat by LF-NMR[J]. Science and Technology of Food Industry,2009,98(1):322−325.
    [20]
    闵连吉. 肉类食品工艺学[M]. 北京: 中国商业出版社, 1992.

    Min L J. Meat Technology[M]. BeiJing: China Commerce and Trade Press, 1992.
    [21]
    Bertram H C, Andersen H J, Karlsson A H. Comparative study of low-field NMR relaxation measurements and two traditional methods in the determination of water holding capacity of pork[J]. Meat Sci,2001,57(2):125−132. doi: 10.1016/S0309-1740(00)00080-2
    [22]
    李春, 张录达, 任发政, 等. 利用低场核磁共振研究冷却条件对猪肉保水性的影响[J]. 农业工程学报,2012,28(23):243−249. [Li C, Zhang L D, Ren F Z, et al. Study on different chilling factors influencing water-holding capacity of pork based on low-field nuclear magnetic resonance(LF-NMR)[J]. Transactions of the Chinese Society of Agricultural Engineering,2012,28(23):243−249.
    [23]
    Sánchez-Valencia J, Sánchez-Alonso I, Martinez I, et al. Estimation of frozen storage time or temperature by kinetic modeling of the Kramer shear resistance and water holding capacity (WHC) of hake (Merluccius merluccius L.) muscle[J]. Journal of Food Engineering,2014,120(1):37−43.
    [24]
    Kominami Y, Watanabe M, Suzuki T. Effects of postmortem physiological changeson ice crystal morphology in the fish muscle tissue[J]. Trans Jsrae,2014,31(3):47−56.
    [25]
    Maureen D, Sánchez-Alonso I, Guillaume D, et al. LF1 HNMR T2 relaxation rate as affected by water addition, NaCl and pH in fresh, frozen and cooked minced hake[J]. Food Chemistry,2019,277:229−237. doi: 10.1016/j.foodchem.2018.10.106
    [26]
    沈春蕾, 张小利, 赵金丽, 等. 糖醇对冻藏南美白对虾的品质保障作用[J]. 包装工程,2019,40(1):15−23. [Shen C L, Zhang X L, Zhao J L, et al. Effect of sugar alcohols on the quality of shrimp (Litopenaeus vannamei) during Frozen Storage[J]. Packaging Engineering,2019,40(1):15−23.
    [27]
    曾俊杰, 熊光权, 付晓燕, 等. 冻藏过程中多糖对肌原纤维蛋白化学作用力的影响[J]. 食品科技,2018,43(6):274−281. [Zeng J J, Xiong G Q, Fu X Y, et al. Effects of different polysaccharides on chemical force of myofibrillar proteins during frozen storage[J]. Food Science and Technology,2018,43(6):274−281.
    [28]
    Cabral A R, Blackmon T L, Miller R K, et al. Relationships between fatty acid composition, trained panel descriptors, and volatile aroma compounds of ground beef patties of brisket, flank and plate with 10%, 20%, and 30% total fat[J]. Journal of animal science,2017,95(3):183−183.
    [29]
    Wang J, Xu L, Xu Z, et al. Liquid chromatography quadrupole time-of-flight mass spectrometry and rapid evaporative ionization mass spectrometry were used to develop a lamb authentication method: A preliminary study[J]. Foods,2020,9(12):125−129.
    [30]
    Gao J, Yang P, Cui Y, et al. Identification of metabonomics changes in longissimus dorsi muscle of finishing pigs following heat stress through LC-MS/MS-based metabonomics method[J]. Animals (Basel),2020,10(1):19−23.
    [31]
    丛艳君, 薛文通. 活性蛋白质和肽的制备及在功能食品中的应用[M]. 北京: 中国轻工业出版社, 2011.

    Cong Y J, Xue W T. Preparation of active proteins and peptides and their application in functional foods[M]. Beijing: China Light Industry Press, 2011.
    [32]
    Burgess C. Topical vitamins[J]. Journal of Drugs in Dermatology,2008,7(7):S2−S6.
    [33]
    Johnston J E, Sepe H A, Miano C L, et al. Honey inhibits lipid oxidation in ready-to-eat ground beef patties[J]. Meat Science,2005,70(4):627−631. doi: 10.1016/j.meatsci.2005.02.011
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